Restriction map for
pFN21AE1689
(Circular) (Six-base) MAPSORT of: pFN21AE1689.seq Check: 2967 from: 1 to: 5302
pFN21AE1689 5302 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2336 2336
Size: 5302
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3112 278
Size: 53 83 186 936 1576 2468
Fragments arranged by size:
2468 1576 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2645 1937
Size: 708 4594
Acc65I G'GTAC_C
Cuts at: 2623 2623
Size: 5302
AclI AA'CG_TT
Cuts at: 1563 3429 3802 1563
Size: 1866 373 3063
Fragments arranged by size:
3063 1866 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2381 2609 3365 4749 5017 1632
Size: 749 228 756 1384 268 1917
Fragments arranged by size:
1917 1384 756 749 268 228
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5105
AflIII A'CryG_T
Cuts at: 1203 4201 5178 1203
Size: 2998 977 1327
Fragments arranged by size:
2998 1327 977
AgeI A'CCGG_T
Cuts at: 4133 4133
Size: 5302
AhdI GACnn_n'nnGTC
Cuts at: 2308 4031 2308
Size: 1723 3579
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5302
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2190 1184
Size: 1006 4296
AlwNI CAG_nnn'CTG
Cuts at: 2335 2431 3045 4617 2335
Size: 96 614 1572 3020
Fragments arranged by size:
3020 1572 614 96
ApaLI G'TGCA_C
Cuts at: 3359 4515 3359
Size: 1156 4146
ApoI r'AATT_y
Cuts at: 1493 2192 2611 2837 2989 5098 1493
Size: 699 419 226 152 2109 1697
Fragments arranged by size:
2109 1697 699 419 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3856 160
Size: 872 2824 1606
Fragments arranged by size:
2824 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5302
AvaI C'yCGr_G
Cuts at: 1870 1943 2091 2109 2375 2627 1870
Size: 73 148 18 266 252 4545
Fragments arranged by size:
4545 266 252 148 73 18
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2046 2079 2046
Size: 33 5269
BamHI G'GATC_C
Cuts at: 2632 2999 2632
Size: 367 4935
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2384 2476 2488 2623 4078
Size: 325 217 627 597 92 12 135 1455
Cuts at: 4078 618
Size: 1842
Fragments arranged by size:
1842 1455 627 597 325 217 135 92
12
BanII G_rGCy'C
Cuts at: 1391 2266 2376 2621 1391
Size: 875 110 245 4072
Fragments arranged by size:
4072 875 245 110
BbeI G_GCGC'C
Cuts at: 2480 2492 2480
Size: 12 5290
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2369 2597 928
Size: 971 470 228 3633
Fragments arranged by size:
3633 971 470 228
BbvCI CC'TCA_GC
Cuts at: 2183 2500 2183
Size: 317 4985
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3493 3527 1903
Size: 34 1556 34 3678
Fragments arranged by size:
3678 1556 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3194 4404 3194
Size: 1210 4092
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5302
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3913 136
Size: 107 122 71 631 605 2241 1525
Fragments arranged by size:
2241 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5297 5297
Size: 5302
BlpI GC'TnA_GC
Cuts at: 2039 2719 2039
Size: 680 4622
Bme1580I G_kGCm'C
Cuts at: 1790 2257 2387 3363 4519 1790
Size: 467 130 976 1156 2573
Fragments arranged by size:
2573 1156 976 467 130
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5302
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2267 2572 3033
Size: 416 53 83 399 24 850 305 461
Cuts at: 3033 3991 442
Size: 958 1753
Fragments arranged by size:
1753 958 850 461 416 399 305 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5302
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2390 3962 1239
Size: 1151 1572 2579
Fragments arranged by size:
2579 1572 1151
Bpu10I CC'TnA_GC
Cuts at: 2183 2500 2183
Size: 317 4985
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3427 4292 4590 3427
Size: 865 298 4139
Fragments arranged by size:
4139 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3965 882
Size: 617 2466 2219
Fragments arranged by size:
2466 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2326 3093 493
Size: 711 1122 767 2702
Fragments arranged by size:
2702 1122 767 711
BsaBI GATnn'nnATC
Cuts at: 5296 5296
Size: 5302
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2477 2489 3109 3491
Size: 53 83 186 936 944 12 620 382
Cuts at: 3491 4126 275
Size: 635 1451
Fragments arranged by size:
1451 944 936 635 620 382 186 83
53 12
BsaWI w'CCGG_w
Cuts at: 3735 4133 4407 4554 4965 3735
Size: 398 274 147 411 4072
Fragments arranged by size:
4072 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5077
Fragments arranged by size:
5077 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2488 1186
Size: 1302 4000
BseYI C'CCAG_C
Cuts at: 2197 2380 2454 4505 5001 2197
Size: 183 74 2051 496 2498
Fragments arranged by size:
2498 2051 496 183 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2160 2268 2348 2531 1163
Size: 573 424 108 80 183 3934
Fragments arranged by size:
3934 573 424 183 108 80
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2752 3513 4541 1540
Size: 275 186 751 761 1028 2301
Fragments arranged by size:
2301 1028 761 751 275 186
BsiHKAI G_wGCw'C
Cuts at: 2136 2266 3363 3448 4519 2136
Size: 130 1097 85 1071 2919
Fragments arranged by size:
2919 1097 1071 130 85
BsmI GAATG_Cn'
Cuts at: 2813 2906 2813
Size: 93 5209
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2257 2266 2376 2387 2621 3363
Size: 399 346 121 9 110 11 234 742
Cuts at: 3363 3448 4519 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 346 234 121 110
85 11 9
BspEI T'CCGG_A
Cuts at: 4965 4965
Size: 5302
BspHI T'CATG_A
Cuts at: 3191 4921 3191
Size: 1730 3572
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2325 2336 2657 844
Size: 1481 11 321 3489
Fragments arranged by size:
3489 1481 321 11
BsrBI CCG'CTC
Cuts at: 1394 1823 2145 2300 3189 1394
Size: 429 322 155 889 3507
Fragments arranged by size:
3507 889 429 322 155
BsrDI GCAATG_nn'
Cuts at: 65 1248 3797 3971 65
Size: 1183 2549 174 1396
Fragments arranged by size:
2549 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2406 2418 2473 3946 4133 1700
Size: 110 596 12 55 1473 187 2869
Fragments arranged by size:
2869 1473 596 187 110 55 12
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5302
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5302
BssSI C'ACGA_G
Cuts at: 3362 4374 3362
Size: 1012 4290
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2335 794
Size: 1541 3761
BstBI TT'CG_AA
Cuts at: 4106 4106
Size: 5302
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5302
BstYI r'GATC_y
Cuts at: 1972 2632 2999 3384 3401 4853 4939 4951 5297
Size: 660 367 385 17 1452 86 12 346
Cuts at: 5297 1972
Size: 1977
Fragments arranged by size:
1977 1452 660 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4750
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4563
Fragments arranged by size:
4563 683 56
BtsI GCAGTG_nn'
Cuts at: 2336 2818 3612 3632 2336
Size: 482 794 20 4006
Fragments arranged by size:
4006 794 482 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5302
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5267
DraI TTT'AAA
Cuts at: 2606 2606
Size: 5302
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4309 809
Size: 507 2993 1802
Fragments arranged by size:
2993 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2048 2238 2749
Size: 54 1425 50 275 18 218 190 511
Cuts at: 2749 3638 5267 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 511 275 218 190 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2749 1537
Size: 275 937 4090
Fragments arranged by size:
4090 937 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2359 3232 1353
Size: 1006 873 3423
Fragments arranged by size:
3423 1006 873
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2171 3889 4263 4409 387
Size: 1064 138 582 1718 374 146 1280
Fragments arranged by size:
1718 1280 1064 582 374 146 138
EcoICRI GAG'CTC
Cuts at: 2264 2264
Size: 5302
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2381 2390 2609 3365 3962 4749 5017
Size: 393 749 9 219 756 597 787 268
Cuts at: 5017 1239
Size: 1524
Fragments arranged by size:
1524 787 756 749 597 393 268 219
9
EcoRI G'AATT_C
Cuts at: 2611 2989 2611
Size: 378 4924
FspI TGC'GCA
Cuts at: 3808 3808
Size: 5302
HaeII r_GCGC'y
Cuts at: 1678 2480 2492 4449 1678
Size: 802 12 1957 2531
Fragments arranged by size:
2531 1957 802 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2131 2163 2294 2326 3943
Size: 32 135 32 559 32 131 32 1617
Cuts at: 3943 3975 4017 4049 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1617 559 135 131 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2646 2892 669
Size: 988 281 708 246 3079
Fragments arranged by size:
3079 988 708 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5302
HpaI GTT'AAC
Cuts at: 2892 2892
Size: 5302
KasI G'GCGC_C
Cuts at: 2476 2488 2476
Size: 12 5290
KpnI G_GTAC'C
Cuts at: 2627 2627
Size: 5302
MfeI C'AATT_G
Cuts at: 2901 2901
Size: 5302
MluI A'CGCG_T
Cuts at: 5178 5178
Size: 5302
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4416 4600 1305
Size: 3111 184 2007
Fragments arranged by size:
3111 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2050 2240 10
Size: 54 1425 343 218 190 3072
Fragments arranged by size:
3072 1425 343 218 190 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3260 3619 3778 518
Size: 648 2094 359 159 2042
Fragments arranged by size:
2094 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2060 2170 2182 2201 2485 2718 3395 4543
Size: 454 110 12 19 284 233 677 1148
Cuts at: 4543 4788 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 454 284 245 233 110
19 12
NaeI GCC'GGC
Cuts at: 2420 2475 2420
Size: 55 5247
NarI GG'CG_CC
Cuts at: 2477 2489 2477
Size: 12 5290
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4750
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5302
NgoMIV G'CCGG_C
Cuts at: 2418 2473 2418
Size: 55 5247
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5302
NotI GC'GGCC_GC
Cuts at: 2749 2749
Size: 5302
NspI r_CATG'y
Cuts at: 1132 1229 2660 4205 1132
Size: 97 1431 1545 2229
Fragments arranged by size:
2229 1545 1431 97
PasI CC'CwG_GG
Cuts at: 1348 2069 1348
Size: 721 4581
PciI A'CATG_T
Cuts at: 4201 4201
Size: 5302
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2081 2218 1108
Size: 973 137 4192
Fragments arranged by size:
4192 973 137
PmeI GTTT'AAAC
Cuts at: 2606 2606
Size: 5302
PmlI CAC'GTG
Cuts at: 2326 2326
Size: 5302
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3371 3403 4884 4916 3371
Size: 32 1481 32 3757
Fragments arranged by size:
3757 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5302
PsiI TTA'TAA
Cuts at: 2872 2872
Size: 5302
PspXI vC'TCGA_Gb
Cuts at: 2091 2091
Size: 5302
PstI C_TGCA'G
Cuts at: 830 2394 2427 2654 830
Size: 1564 33 227 3478
Fragments arranged by size:
3478 1564 227 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5302
PvuII CAG'CTG
Cuts at: 2060 2170 2182 2201 2060
Size: 110 12 19 5161
Fragments arranged by size:
5161 110 19 12
SacI G_AGCT'C
Cuts at: 2266 2266
Size: 5302
SalI G'TCGA_C
Cuts at: 1936 2644 1936
Size: 708 4594
SapI GCTCTTCn'nnn_
Cuts at: 2359 2359
Size: 5302
SbfI CC_TGCA'GG
Cuts at: 2394 2654 2394
Size: 260 5042
ScaI AGT'ACT
Cuts at: 3550 5262 3550
Size: 1712 3590
SfcI C'TryA_G
Cuts at: 826 1045 2267 2390 2423 2650 3785 4466 4657
Size: 219 1222 123 33 227 1135 681 191
Cuts at: 4657 826
Size: 1471
Fragments arranged by size:
1471 1222 1135 681 227 219 191 123
33
SfoI GGC'GCC
Cuts at: 2478 2490 2478
Size: 12 5290
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5302
SgrAI Cr'CCGG_yG
Cuts at: 1700 2473 1700
Size: 773 4529
SmaI CCC'GGG
Cuts at: 1872 2629 1872
Size: 757 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2091 3406 4307 4569 820
Size: 197 926 148 1315 901 262 1553
Fragments arranged by size:
1553 1315 926 901 262 197 148
SnaBI TAC'GTA
Cuts at: 493 3093 493
Size: 2600 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5302
SphI G_CATG'C
Cuts at: 1132 2660 1132
Size: 1528 3774
SspI AAT'ATT
Cuts at: 5 52 3226 5
Size: 47 3174 2081
Fragments arranged by size:
3174 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5302
StyI C'CwwG_G
Cuts at: 513 1065 2741 513
Size: 552 1676 3074
Fragments arranged by size:
3074 1676 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3329 3346 3499 3684 4129 325
Size: 1255 1749 17 153 185 445 1498
Fragments arranged by size:
1749 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3548 5260 96
Size: 274 80 33 51 1443 1571 1712 138
Fragments arranged by size:
1712 1571 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5262 5294 5262
Size: 32 5270
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5302
XbaI T'CTAG_A
Cuts at: 2638 2638
Size: 5302
XhoI C'TCGA_G
Cuts at: 1943 2091 1943
Size: 148 5154
XmaI C'CCGG_G
Cuts at: 1870 2627 1870
Size: 757 4545
XmnI GAAnn'nnTTC
Cuts at: 3431 3431
Size: 5302
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3110 276
Size: 53 83 186 936 1576 2468
Fragments arranged by size:
2468 1576 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PpuMI
PspOMI PsrI RsrII SacII SanDI SexAI SfiI SrfI
SwaI XcmI