Restriction map for
pFN21AE1684
(Circular) (Six-base) MAPSORT of: pFN21AE1684.seq Check: 9982 from: 1 to: 5293
pFN21AE1684 5293 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2251 2251
Size: 5293
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3103 278
Size: 53 83 186 936 1567 2468
Fragments arranged by size:
2468 1567 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2636 1937
Size: 699 4594
Acc65I G'GTAC_C
Cuts at: 2614 2614
Size: 5293
AclI AA'CG_TT
Cuts at: 1563 3420 3793 1563
Size: 1857 373 3063
Fragments arranged by size:
3063 1857 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2222 2330 3356 4740 5008 1632
Size: 590 108 1026 1384 268 1917
Fragments arranged by size:
1917 1384 1026 590 268 108
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5096
AflIII A'CryG_T
Cuts at: 1203 4192 5169 1203
Size: 2989 977 1327
Fragments arranged by size:
2989 1327 977
AgeI A'CCGG_T
Cuts at: 4124 4124
Size: 5293
AhdI GACnn_n'nnGTC
Cuts at: 4022 4022
Size: 5293
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5293
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2042 2128 1184
Size: 858 86 4349
Fragments arranged by size:
4349 858 86
AlwNI CAG_nnn'CTG
Cuts at: 2072 2152 2241 3036 4608 2072
Size: 80 89 795 1572 2757
Fragments arranged by size:
2757 1572 795 89 80
ApaI G_GGCC'C
Cuts at: 2439 2439
Size: 5293
ApaLI G'TGCA_C
Cuts at: 3350 4506 3350
Size: 1156 4137
ApoI r'AATT_y
Cuts at: 1493 2602 2828 2980 5089 1493
Size: 1109 226 152 2109 1697
Fragments arranged by size:
2109 1697 1109 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3847 160
Size: 872 2815 1606
Fragments arranged by size:
2815 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5293
AvaI C'yCGr_G
Cuts at: 1870 1943 2445 2513 2618 1870
Size: 73 502 68 105 4545
Fragments arranged by size:
4545 502 105 73 68
BamHI G'GATC_C
Cuts at: 2623 2990 2623
Size: 367 4926
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2614 4069 618
Size: 325 217 627 827 1455 1842
Fragments arranged by size:
1842 1455 827 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2095 2439 2612 1391
Size: 704 344 173 4072
Fragments arranged by size:
4072 704 344 173
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4322
BbvCI CC'TCA_GC
Cuts at: 2421 2421
Size: 5293
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3484 3518 1903
Size: 34 1547 34 3678
Fragments arranged by size:
3678 1547 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2423 3185 4395 2423
Size: 762 1210 3321
Fragments arranged by size:
3321 1210 762
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5293
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3904 136
Size: 107 122 71 631 605 2232 1525
Fragments arranged by size:
2232 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5288 5288
Size: 5293
BlpI GC'TnA_GC
Cuts at: 2292 2710 2292
Size: 418 4875
Bme1580I G_kGCm'C
Cuts at: 1790 2439 3354 4510 1790
Size: 649 915 1156 2573
Fragments arranged by size:
2573 1156 915 649
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5293
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2119 3024 3982
Size: 416 53 83 399 24 702 905 958
Cuts at: 3982 442
Size: 1753
Fragments arranged by size:
1753 958 905 702 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5293
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2082 2339 2462 3953 1239
Size: 843 257 123 1491 2579
Fragments arranged by size:
2579 1491 843 257 123
Bpu10I CC'TnA_GC
Cuts at: 2421 2421
Size: 5293
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3418 4283 4581 3418
Size: 865 298 4130
Fragments arranged by size:
4130 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2550 3956 882
Size: 617 1051 1406 2219
Fragments arranged by size:
2219 1406 1051 617
BsaAI yAC'GTr
Cuts at: 493 1204 3084 493
Size: 711 1880 2702
Fragments arranged by size:
2702 1880 711
BsaBI GATnn'nnATC
Cuts at: 5287 5287
Size: 5293
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3100 3482 4117 275
Size: 53 83 186 936 1567 382 635 1451
Fragments arranged by size:
1567 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3726 4124 4398 4545 4956 3726
Size: 398 274 147 411 4063
Fragments arranged by size:
4063 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5068
Fragments arranged by size:
5068 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2537 1186
Size: 1351 3942
BseYI C'CCAG_C
Cuts at: 2341 2397 4496 4992 2341
Size: 56 2099 496 2642
Fragments arranged by size:
2642 2099 496 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2366 2453 1163
Size: 573 630 87 4003
Fragments arranged by size:
4003 630 573 87
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2743 3504 4532 1540
Size: 275 186 742 761 1028 2301
Fragments arranged by size:
2301 1028 761 742 275 186
BsiHKAI G_wGCw'C
Cuts at: 3354 3439 4510 3354
Size: 85 1071 4137
Fragments arranged by size:
4137 1071 85
BsmI GAATG_Cn'
Cuts at: 2804 2897 2804
Size: 93 5200
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2095 2439 2612 3354 3439 4510 1391
Size: 399 305 344 173 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 344 305 173 85
BspEI T'CCGG_A
Cuts at: 4956 4956
Size: 5293
BspHI T'CATG_A
Cuts at: 3182 4912 3182
Size: 1730 3563
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2251 2648 844
Size: 1407 397 3489
Fragments arranged by size:
3489 1407 397
BsrBI CCG'CTC
Cuts at: 1394 1823 2220 3180 1394
Size: 429 397 960 3507
Fragments arranged by size:
3507 960 429 397
BsrDI GCAATG_nn'
Cuts at: 65 1248 3788 3962 65
Size: 1183 2540 174 1396
Fragments arranged by size:
2540 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2472 3937 4124 1700
Size: 110 662 1465 187 2869
Fragments arranged by size:
2869 1465 662 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5293
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5293
BssSI C'ACGA_G
Cuts at: 3353 4365 3353
Size: 1012 4281
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5293
BstBI TT'CG_AA
Cuts at: 4097 4097
Size: 5293
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5293
BstYI r'GATC_y
Cuts at: 1972 2623 2990 3375 3392 4844 4930 4942 5288
Size: 651 367 385 17 1452 86 12 346
Cuts at: 5288 1972
Size: 1977
Fragments arranged by size:
1977 1452 651 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2249 2249
Size: 5293
BtgI C'CryG_G
Cuts at: 513 1065 2086 2277 2427 513
Size: 552 1021 191 150 3379
Fragments arranged by size:
3379 1021 552 191 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4554
Fragments arranged by size:
4554 683 56
BtsI GCAGTG_nn'
Cuts at: 2052 2809 3603 3623 2052
Size: 757 794 20 3722
Fragments arranged by size:
3722 794 757 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5293
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5258
DraI TTT'AAA
Cuts at: 2597 2597
Size: 5293
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2588 4300 809
Size: 507 1272 1712 1802
Fragments arranged by size:
1802 1712 1272 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2740 3629
Size: 54 1425 50 275 18 210 700 889
Cuts at: 3629 5258 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 700 275 210 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2740 1537
Size: 275 928 4090
Fragments arranged by size:
4090 928 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2181 2234 2543 3223 1353
Size: 828 53 309 680 3423
Fragments arranged by size:
3423 828 680 309 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2531 3880 4254 4400 387
Size: 1064 138 942 1349 374 146 1280
Fragments arranged by size:
1349 1280 1064 942 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2082 2222 2330 2339 2462 3356 3953
Size: 393 450 140 108 9 123 894 597
Cuts at: 3953 4740 5008 1239
Size: 787 268 1524
Fragments arranged by size:
1524 894 787 597 450 393 268 140
123 108 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2164 2164
Size: 5293
EcoO109I rG'GnC_Cy
Cuts at: 2238 2245 2435 2442 2485 2581 2238
Size: 7 190 7 43 96 4950
Fragments arranged by size:
4950 190 96 43 7 7
EcoRI G'AATT_C
Cuts at: 2602 2980 2602
Size: 378 4915
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2210 2210
Size: 5293
FspI TGC'GCA
Cuts at: 3799 3799
Size: 5293
HaeII r_GCGC'y
Cuts at: 1678 2275 4440 1678
Size: 597 2165 2531
Fragments arranged by size:
2531 2165 597
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3934 3966 4008 4040 1373
Size: 32 135 32 2362 32 42 32 2626
Fragments arranged by size:
2626 2362 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2637 2883 669
Size: 988 281 699 246 3079
Fragments arranged by size:
3079 988 699 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5293
HpaI GTT'AAC
Cuts at: 2883 2883
Size: 5293
KpnI G_GTAC'C
Cuts at: 2618 2618
Size: 5293
MfeI C'AATT_G
Cuts at: 2892 2892
Size: 5293
MluI A'CGCG_T
Cuts at: 5169 5169
Size: 5293
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4407 4591 1305
Size: 3102 184 2007
Fragments arranged by size:
3102 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3471
Fragments arranged by size:
3471 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2024 3251 3610 3769 518
Size: 648 858 1227 359 159 2042
Fragments arranged by size:
2042 1227 858 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2088 2257 2330 2577 2709 3386 4534 4779
Size: 482 169 73 247 132 677 1148 245
Cuts at: 4779 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 482 247 245 169 132
73
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4741
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5293
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5293
NotI GC'GGCC_GC
Cuts at: 2740 2740
Size: 5293
NspI r_CATG'y
Cuts at: 1132 1229 2023 2651 4196 1132
Size: 97 794 628 1545 2229
Fragments arranged by size:
2229 1545 794 628 97
PasI CC'CwG_GG
Cuts at: 1348 2347 1348
Size: 999 4294
PciI A'CATG_T
Cuts at: 4192 4192
Size: 5293
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2241 1108
Size: 1133 4160
PfoI T'CCnGG_A
Cuts at: 2097 2478 2097
Size: 381 4912
PmeI GTTT'AAAC
Cuts at: 2597 2597
Size: 5293
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3362 3394 4875 4907 3362
Size: 32 1481 32 3748
Fragments arranged by size:
3748 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2238 2245 2442 2581 2238
Size: 7 197 139 4950
Fragments arranged by size:
4950 197 139 7
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5293
PsiI TTA'TAA
Cuts at: 2863 2863
Size: 5293
PspOMI G'GGCC_C
Cuts at: 2435 2435
Size: 5293
PspXI vC'TCGA_Gb
Cuts at: 2513 2513
Size: 5293
PstI C_TGCA'G
Cuts at: 830 2157 2262 2645 830
Size: 1327 105 383 3478
Fragments arranged by size:
3478 1327 383 105
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5293
PvuII CAG'CTG
Cuts at: 2257 2257
Size: 5293
SacII CC_GC'GG
Cuts at: 2089 2089
Size: 5293
SalI G'TCGA_C
Cuts at: 1936 2635 1936
Size: 699 4594
SbfI CC_TGCA'GG
Cuts at: 2645 2645
Size: 5293
ScaI AGT'ACT
Cuts at: 3541 5253 3541
Size: 1712 3581
SfcI C'TryA_G
Cuts at: 826 1045 2153 2258 2641 3776 4457 4648 826
Size: 219 1108 105 383 1135 681 191 1471
Fragments arranged by size:
1471 1135 1108 681 383 219 191 105
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5293
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5293
SmaI CCC'GGG
Cuts at: 1872 2447 2620 1872
Size: 575 173 4545
Fragments arranged by size:
4545 575 173
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2513 3397 4298 4560 820
Size: 197 926 570 884 901 262 1553
Fragments arranged by size:
1553 926 901 884 570 262 197
SnaBI TAC'GTA
Cuts at: 493 3084 493
Size: 2591 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5293
SphI G_CATG'C
Cuts at: 1132 2023 2651 1132
Size: 891 628 3774
Fragments arranged by size:
3774 891 628
SspI AAT'ATT
Cuts at: 5 52 3217 5
Size: 47 3165 2081
Fragments arranged by size:
3165 2081 47
StuI AGG'CCT
Cuts at: 1517 2159 2519 1517
Size: 642 360 4291
Fragments arranged by size:
4291 642 360
StyI C'CwwG_G
Cuts at: 513 1065 2732 513
Size: 552 1667 3074
Fragments arranged by size:
3074 1667 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3320 3337 3490 3675 4120 325
Size: 1255 1740 17 153 185 445 1498
Fragments arranged by size:
1740 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3539 5251 96
Size: 274 80 33 51 1443 1562 1712 138
Fragments arranged by size:
1712 1562 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2198 2230 5253 5285 2198
Size: 32 3023 32 2206
Fragments arranged by size:
3023 2206 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5293
XbaI T'CTAG_A
Cuts at: 2629 2629
Size: 5293
XhoI C'TCGA_G
Cuts at: 1943 2513 1943
Size: 570 4723
XmaI C'CCGG_G
Cuts at: 1870 2445 2618 1870
Size: 575 173 4545
Fragments arranged by size:
4545 575 173
XmnI GAAnn'nnTTC
Cuts at: 3422 3422
Size: 5293
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3101 276
Size: 53 83 186 936 1567 2468
Fragments arranged by size:
2468 1567 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PsrI RsrII SacI SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI