Restriction map for
pFN21AE1681
(Circular) (Six-base) MAPSORT of: pFN21AE1681.seq Check: 572 from: 1 to: 5287
pFN21AE1681 5287 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3097 278
Size: 53 83 186 936 1561 2468
Fragments arranged by size:
2468 1561 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2630 1937
Size: 693 4594
Acc65I G'GTAC_C
Cuts at: 2608 2608
Size: 5287
AclI AA'CG_TT
Cuts at: 1563 3414 3787 1563
Size: 1851 373 3063
Fragments arranged by size:
3063 1851 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2272 3350 4734 5002 1632
Size: 640 1078 1384 268 1917
Fragments arranged by size:
1917 1384 1078 640 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5090
AflIII A'CryG_T
Cuts at: 1203 4186 5163 1203
Size: 2983 977 1327
Fragments arranged by size:
2983 1327 977
AgeI A'CCGG_T
Cuts at: 4118 4118
Size: 5287
AhdI GACnn_n'nnGTC
Cuts at: 4016 4016
Size: 5287
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5287
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5287
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2463 2495 2463
Size: 32 5255
AlwNI CAG_nnn'CTG
Cuts at: 3030 4602 3030
Size: 1572 3715
ApaLI G'TGCA_C
Cuts at: 3344 4500 3344
Size: 1156 4131
ApoI r'AATT_y
Cuts at: 1493 2596 2822 2974 5083 1493
Size: 1103 226 152 2109 1697
Fragments arranged by size:
2109 1697 1103 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3841 160
Size: 872 2809 1606
Fragments arranged by size:
2809 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5287
AvaI C'yCGr_G
Cuts at: 1870 1943 2612 1870
Size: 73 669 4545
Fragments arranged by size:
4545 669 73
BamHI G'GATC_C
Cuts at: 2617 2984 2617
Size: 367 4920
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2608 4063 618
Size: 325 217 627 821 1455 1842
Fragments arranged by size:
1842 1455 821 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2606 1391
Size: 1215 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4316
BbvCI CC'TCA_GC
Cuts at: 2036 2519 2036
Size: 483 4804
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3478 3512 1903
Size: 34 1541 34 3678
Fragments arranged by size:
3678 1541 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3179 4389 3179
Size: 1210 4077
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5287
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3898 136
Size: 107 122 71 631 605 2226 1525
Fragments arranged by size:
2226 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5282 5282
Size: 5287
BlpI GC'TnA_GC
Cuts at: 2187 2249 2704 2187
Size: 62 455 4770
Fragments arranged by size:
4770 455 62
Bme1580I G_kGCm'C
Cuts at: 1790 2068 3348 4504 1790
Size: 278 1280 1156 2573
Fragments arranged by size:
2573 1280 1156 278
BmgBI CAC'GTC
Cuts at: 1318 2071 2491 1318
Size: 753 420 4114
Fragments arranged by size:
4114 753 420
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2165 3018 3976
Size: 416 53 83 399 24 748 853 958
Cuts at: 3976 442
Size: 1753
Fragments arranged by size:
1753 958 853 748 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5287
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2432 2542 3947 1239
Size: 1193 110 1405 2579
Fragments arranged by size:
2579 1405 1193 110
Bpu10I CC'TnA_GC
Cuts at: 2036 2519 2036
Size: 483 4804
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3412 4277 4575 3412
Size: 865 298 4124
Fragments arranged by size:
4124 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3950 882
Size: 617 2451 2219
Fragments arranged by size:
2451 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3078 493
Size: 711 1874 2702
Fragments arranged by size:
2702 1874 711
BsaBI GATnn'nnATC
Cuts at: 5281 5281
Size: 5287
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3094 3476 4111 275
Size: 53 83 186 936 1561 382 635 1451
Fragments arranged by size:
1561 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3720 4118 4392 4539 4950 3720
Size: 398 274 147 411 4057
Fragments arranged by size:
4057 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2368 2398 1178
Size: 30 165 30 965 30 4067
Fragments arranged by size:
4067 965 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5287
BseYI C'CCAG_C
Cuts at: 2163 2179 2305 2502 2513 4490 4986 2163
Size: 16 126 197 11 1977 496 2464
Fragments arranged by size:
2464 1977 496 197 126 16 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2417 1163
Size: 573 681 4033
Fragments arranged by size:
4033 681 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2737 3498 4526 1540
Size: 275 186 736 761 1028 2301
Fragments arranged by size:
2301 1028 761 736 275 186
BsiHKAI G_wGCw'C
Cuts at: 2331 2360 2381 2460 3348 3433 4504 2331
Size: 29 21 79 888 85 1071 3114
Fragments arranged by size:
3114 1071 888 85 79 29 21
BsmI GAATG_Cn'
Cuts at: 2798 2891 2798
Size: 93 5194
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2068 2331 2360 2381 2460 2606 3348
Size: 399 278 263 29 21 79 146 742
Cuts at: 3348 3433 4504 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 278 263 146 85
79 29 21
BspEI T'CCGG_A
Cuts at: 4950 4950
Size: 5287
BspHI T'CATG_A
Cuts at: 3176 4906 3176
Size: 1730 3557
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2642 844
Size: 1798 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3174 1394
Size: 429 1351 3507
Fragments arranged by size:
3507 1351 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2317 3782 3956 65
Size: 1183 1069 1465 174 1396
Fragments arranged by size:
1465 1396 1183 1069 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3931 4118 1700
Size: 110 2121 187 2869
Fragments arranged by size:
2869 2121 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5287
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5287
BssSI C'ACGA_G
Cuts at: 2459 3347 4359 2459
Size: 888 1012 3387
Fragments arranged by size:
3387 1012 888
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5287
BstBI TT'CG_AA
Cuts at: 4091 4091
Size: 5287
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5287
BstYI r'GATC_y
Cuts at: 1972 2617 2984 3369 3386 4838 4924 4936 5282
Size: 645 367 385 17 1452 86 12 346
Cuts at: 5282 1972
Size: 1977
Fragments arranged by size:
1977 1452 645 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4735
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4548
Fragments arranged by size:
4548 683 56
BtsI GCAGTG_nn'
Cuts at: 2124 2198 2803 3597 3617 2124
Size: 74 605 794 20 3794
Fragments arranged by size:
3794 794 605 74 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5287
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5252
DraI TTT'AAA
Cuts at: 2591 2591
Size: 5287
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4294 809
Size: 507 2978 1802
Fragments arranged by size:
2978 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2216 2734 3623
Size: 54 1425 50 275 18 386 518 889
Cuts at: 3623 5252 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 518 386 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2734 1537
Size: 275 922 4090
Fragments arranged by size:
4090 922 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3217 1353
Size: 1864 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3874 4248 4394 387
Size: 1064 138 2285 374 146 1280
Fragments arranged by size:
2285 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2272 2432 2542 3350 3947 4734 5002
Size: 393 640 160 110 808 597 787 268
Cuts at: 5002 1239
Size: 1524
Fragments arranged by size:
1524 808 787 640 597 393 268 160
110
EcoO109I rG'GnC_Cy
Cuts at: 2050 2149 2050
Size: 99 5188
EcoRI G'AATT_C
Cuts at: 2596 2974 2596
Size: 378 4909
FspI TGC'GCA
Cuts at: 3793 3793
Size: 5287
HaeII r_GCGC'y
Cuts at: 1678 4434 1678
Size: 2756 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3928 3960 4002 4034 1373
Size: 32 135 32 2356 32 42 32 2626
Fragments arranged by size:
2626 2356 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2631 2877 669
Size: 988 281 693 246 3079
Fragments arranged by size:
3079 988 693 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5287
HpaI GTT'AAC
Cuts at: 2877 2877
Size: 5287
KpnI G_GTAC'C
Cuts at: 2612 2612
Size: 5287
MfeI C'AATT_G
Cuts at: 2886 2886
Size: 5287
MluI A'CGCG_T
Cuts at: 5163 5163
Size: 5287
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4401 4585 1305
Size: 3096 184 2007
Fragments arranged by size:
3096 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2218 10
Size: 54 1425 343 386 3079
Fragments arranged by size:
3079 1425 386 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3245 3604 3763 518
Size: 648 843 1236 359 159 2042
Fragments arranged by size:
2042 1236 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2120 2392 2419 2703 3380 4528 4773 1606
Size: 514 272 27 284 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 514 284 272 245 27
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4735
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5287
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5287
NotI GC'GGCC_GC
Cuts at: 2734 2734
Size: 5287
NspI r_CATG'y
Cuts at: 1132 1229 2645 4190 1132
Size: 97 1416 1545 2229
Fragments arranged by size:
2229 1545 1416 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5287
PciI A'CATG_T
Cuts at: 4186 4186
Size: 5287
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2240 1108
Size: 1132 4155
PmeI GTTT'AAAC
Cuts at: 2591 2591
Size: 5287
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3356 3388 4869 4901 3356
Size: 32 1481 32 3742
Fragments arranged by size:
3742 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2050 2149 2050
Size: 99 5188
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5287
PsiI TTA'TAA
Cuts at: 2857 2857
Size: 5287
PstI C_TGCA'G
Cuts at: 830 2273 2639 830
Size: 1443 366 3478
Fragments arranged by size:
3478 1443 366
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5287
PvuII CAG'CTG
Cuts at: 2120 2392 2419 2120
Size: 272 27 4988
Fragments arranged by size:
4988 272 27
SalI G'TCGA_C
Cuts at: 1936 2629 1936
Size: 693 4594
SbfI CC_TGCA'GG
Cuts at: 2639 2639
Size: 5287
ScaI AGT'ACT
Cuts at: 3535 5247 3535
Size: 1712 3575
SfcI C'TryA_G
Cuts at: 826 1045 2269 2635 3770 4451 4642 826
Size: 219 1224 366 1135 681 191 1471
Fragments arranged by size:
1471 1224 1135 681 366 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5287
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5287
SmaI CCC'GGG
Cuts at: 1872 2614 1872
Size: 742 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3391 4292 4554 820
Size: 197 926 1448 901 262 1553
Fragments arranged by size:
1553 1448 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3078 493
Size: 2585 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5287
SphI G_CATG'C
Cuts at: 1132 2645 1132
Size: 1513 3774
SspI AAT'ATT
Cuts at: 5 52 3211 5
Size: 47 3159 2081
Fragments arranged by size:
3159 2081 47
StuI AGG'CCT
Cuts at: 1517 2402 1517
Size: 885 4402
StyI C'CwwG_G
Cuts at: 513 1065 2403 2726 513
Size: 552 1338 323 3074
Fragments arranged by size:
3074 1338 552 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2555 3314 3331 3484 3669 4114 325
Size: 1255 975 759 17 153 185 445 1498
Fragments arranged by size:
1498 1255 975 759 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3533 5245 96
Size: 274 80 33 51 1443 1556 1712 138
Fragments arranged by size:
1712 1556 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5247 5279 5247
Size: 32 5255
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5287
XbaI T'CTAG_A
Cuts at: 2623 2623
Size: 5287
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2343 2510 2343
Size: 167 5120
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5287
XmaI C'CCGG_G
Cuts at: 1870 2612 1870
Size: 742 4545
XmnI GAAnn'nnTTC
Cuts at: 3416 3416
Size: 5287
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3095 276
Size: 53 83 186 936 1561 2468
Fragments arranged by size:
2468 1561 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI