Restriction map for
pFN21AE1666
(Circular) (Six-base) MAPSORT of: pFN21AE1666.seq Check: 3554 from: 1 to: 5260
pFN21AE1666 5260 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2282 3070 278
Size: 53 83 186 936 746 788 2468
Fragments arranged by size:
2468 936 788 746 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2603 1937
Size: 666 4594
Acc65I G'GTAC_C
Cuts at: 2581 2581
Size: 5260
AclI AA'CG_TT
Cuts at: 1563 3387 3760 1563
Size: 1824 373 3063
Fragments arranged by size:
3063 1824 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3323 4707 4975 1632
Size: 1691 1384 268 1917
Fragments arranged by size:
1917 1691 1384 268
AfeI AGC'GCT
Cuts at: 2162 2162
Size: 5260
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5063
AflIII A'CryG_T
Cuts at: 1203 4159 5136 1203
Size: 2956 977 1327
Fragments arranged by size:
2956 1327 977
AgeI A'CCGG_T
Cuts at: 4091 4091
Size: 5260
AhdI GACnn_n'nnGTC
Cuts at: 3989 3989
Size: 5260
AleI CACnn'nnGTG
Cuts at: 1166 2147 2483 1166
Size: 981 336 3943
Fragments arranged by size:
3943 981 336
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2049 2081 2415 1184
Size: 865 32 334 4029
Fragments arranged by size:
4029 865 334 32
AlwNI CAG_nnn'CTG
Cuts at: 3003 4575 3003
Size: 1572 3688
ApaLI G'TGCA_C
Cuts at: 3317 4473 3317
Size: 1156 4104
ApoI r'AATT_y
Cuts at: 1493 2569 2795 2947 5056 1493
Size: 1076 226 152 2109 1697
Fragments arranged by size:
2109 1697 1076 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3814 160
Size: 872 2782 1606
Fragments arranged by size:
2782 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5260
AvaI C'yCGr_G
Cuts at: 1870 1943 2585 1870
Size: 73 642 4545
Fragments arranged by size:
4545 642 73
BamHI G'GATC_C
Cuts at: 2590 2957 2590
Size: 367 4893
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2115 2318 2581 4036 618
Size: 325 217 627 328 203 263 1455 1842
Fragments arranged by size:
1842 1455 627 328 325 263 217 203
BanII G_rGCy'C
Cuts at: 1391 2387 2453 2579 1391
Size: 996 66 126 4072
Fragments arranged by size:
4072 996 126 66
BbeI G_GCGC'C
Cuts at: 2119 2322 2119
Size: 203 5057
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2452 928
Size: 971 553 3736
Fragments arranged by size:
3736 971 553
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2050 2084 3451 3485 1903
Size: 34 113 34 1367 34 3678
Fragments arranged by size:
3678 1367 113 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3152 4362 3152
Size: 1210 4050
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5260
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3871 136
Size: 107 122 71 631 605 2199 1525
Fragments arranged by size:
2199 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5255 5255
Size: 5260
BlpI GC'TnA_GC
Cuts at: 2051 2677 2051
Size: 626 4634
Bme1580I G_kGCm'C
Cuts at: 1790 3321 4477 1790
Size: 1531 1156 2573
Fragments arranged by size:
2573 1531 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5260
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2991 3949 442
Size: 416 53 83 399 24 1574 958 1753
Fragments arranged by size:
1753 1574 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5260
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 2158 2430 3920 1239
Size: 890 29 272 1490 2579
Fragments arranged by size:
2579 1490 890 272 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3385 4250 4548 3385
Size: 865 298 4097
Fragments arranged by size:
4097 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3923 882
Size: 617 2424 2219
Fragments arranged by size:
2424 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3051 493
Size: 711 1847 2702
Fragments arranged by size:
2702 1847 711
BsaBI GATnn'nnATC
Cuts at: 5254 5254
Size: 5260
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2116 2279 2319 3067
Size: 53 83 186 936 583 163 40 748
Cuts at: 3067 3449 4084 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 748 635 583 382 186 163
83 53 40
BsaWI w'CCGG_w
Cuts at: 2087 3693 4091 4365 4512 4923 2087
Size: 1606 398 274 147 411 2424
Fragments arranged by size:
2424 1606 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5035
Fragments arranged by size:
5035 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2552 1186
Size: 1366 3894
BseYI C'CCAG_C
Cuts at: 2386 2470 4463 4959 2386
Size: 84 1993 496 2687
Fragments arranged by size:
2687 1993 496 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2112 1163
Size: 573 376 4311
Fragments arranged by size:
4311 573 376
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2457 2710 3471 4499 1540
Size: 275 186 456 253 761 1028 2301
Fragments arranged by size:
2301 1028 761 456 275 253 186
BsiHKAI G_wGCw'C
Cuts at: 2144 2367 3321 3406 4477 2144
Size: 223 954 85 1071 2927
Fragments arranged by size:
2927 1071 954 223 85
BsiWI C'GTAC_G
Cuts at: 2206 2206
Size: 5260
BsmI GAATG_Cn'
Cuts at: 2771 2864 2771
Size: 93 5167
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2144 2367 2387 2453 2579 3321 3406
Size: 399 354 223 20 66 126 742 85
Cuts at: 3406 4477 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 354 223 126 85
66 20
BspEI T'CCGG_A
Cuts at: 4923 4923
Size: 5260
BspHI T'CATG_A
Cuts at: 3149 4879 3149
Size: 1730 3530
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2615 844
Size: 1771 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2055 2113 3147 1394
Size: 429 232 58 1034 3507
Fragments arranged by size:
3507 1034 429 232 58
BsrDI GCAATG_nn'
Cuts at: 65 1248 3755 3929 65
Size: 1183 2507 174 1396
Fragments arranged by size:
2507 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3904 4091 1700
Size: 110 2094 187 2869
Fragments arranged by size:
2869 2094 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5260
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5260
BssSI C'ACGA_G
Cuts at: 3320 4332 3320
Size: 1012 4248
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5260
BstBI TT'CG_AA
Cuts at: 4064 4064
Size: 5260
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5260
BstXI CCAn_nnnn'nTGG
Cuts at: 2485 2485
Size: 5260
BstYI r'GATC_y
Cuts at: 1972 2590 2957 3342 3359 4811 4897 4909 5255
Size: 618 367 385 17 1452 86 12 346
Cuts at: 5255 1972
Size: 1977
Fragments arranged by size:
1977 1452 618 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2556 513
Size: 552 939 552 3217
Fragments arranged by size:
3217 939 552 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4521
Fragments arranged by size:
4521 683 56
BtsI GCAGTG_nn'
Cuts at: 2776 3570 3590 2776
Size: 794 20 4446
Fragments arranged by size:
4446 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5260
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5225
DraI TTT'AAA
Cuts at: 2564 2564
Size: 5260
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4267 809
Size: 507 2951 1802
Fragments arranged by size:
2951 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2371 2454 2707
Size: 54 1425 50 275 18 541 83 253
Cuts at: 2707 3596 5225 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 541 275 253 83 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2454 2707 1537
Size: 275 642 253 4090
Fragments arranged by size:
4090 642 275 253
EarI CTCTTCn'nnn_
Cuts at: 1353 3190 1353
Size: 1837 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2057 3847 4221 4367 387
Size: 1064 138 468 1790 374 146 1280
Fragments arranged by size:
1790 1280 1064 468 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2158 2430 3323 3920 4707 4975
Size: 393 497 29 272 893 597 787 268
Cuts at: 4975 1239
Size: 1524
Fragments arranged by size:
1524 893 787 597 497 393 272 268
29
EcoRI G'AATT_C
Cuts at: 2569 2947 2569
Size: 378 4882
FspI TGC'GCA
Cuts at: 2342 3766 2342
Size: 1424 3836
HaeII r_GCGC'y
Cuts at: 1678 2119 2164 2203 2322 4407 1678
Size: 441 45 39 119 2085 2531
Fragments arranged by size:
2531 2085 441 119 45 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3901 3933 3975 4007 1373
Size: 32 135 32 2329 32 42 32 2626
Fragments arranged by size:
2626 2329 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2604 2850 669
Size: 988 281 666 246 3079
Fragments arranged by size:
3079 988 666 281 246
HindIII A'AGCT_T
Cuts at: 748 2357 748
Size: 1609 3651
HpaI GTT'AAC
Cuts at: 2850 2850
Size: 5260
KasI G'GCGC_C
Cuts at: 2115 2318 2115
Size: 203 5057
KpnI G_GTAC'C
Cuts at: 2585 2585
Size: 5260
MfeI C'AATT_G
Cuts at: 2859 2859
Size: 5260
MluI A'CGCG_T
Cuts at: 5136 5136
Size: 5260
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4374 4558 1305
Size: 3069 184 2007
Fragments arranged by size:
3069 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3438
Fragments arranged by size:
3438 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2147 2397 2483 3218 3577 3736 518
Size: 648 981 250 86 735 359 159 2042
Fragments arranged by size:
2042 981 735 648 359 250 159 86
MspA1I CmG'CkG
Cuts at: 1606 2676 3353 4501 4746 1606
Size: 1070 677 1148 245 2120
Fragments arranged by size:
2120 1148 1070 677 245
NarI GG'CG_CC
Cuts at: 2116 2319 2116
Size: 203 5057
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3769
Fragments arranged by size:
3769 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5260
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5260
NotI GC'GGCC_GC
Cuts at: 2707 2707
Size: 5260
NspI r_CATG'y
Cuts at: 1132 1229 2618 4163 1132
Size: 97 1389 1545 2229
Fragments arranged by size:
2229 1545 1389 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5260
PciI A'CATG_T
Cuts at: 4159 4159
Size: 5260
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5260
PmeI GTTT'AAAC
Cuts at: 2564 2564
Size: 5260
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3329 3361 4842 4874 3329
Size: 32 1481 32 3715
Fragments arranged by size:
3715 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5260
PsiI TTA'TAA
Cuts at: 2830 2830
Size: 5260
PstI C_TGCA'G
Cuts at: 830 2547 2612 830
Size: 1717 65 3478
Fragments arranged by size:
3478 1717 65
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5260
SalI G'TCGA_C
Cuts at: 1936 2602 1936
Size: 666 4594
SbfI CC_TGCA'GG
Cuts at: 2612 2612
Size: 5260
ScaI AGT'ACT
Cuts at: 2222 3508 5220 2222
Size: 1286 1712 2262
Fragments arranged by size:
2262 1712 1286
SfcI C'TryA_G
Cuts at: 826 1045 2543 2608 3743 4424 4615 826
Size: 219 1498 65 1135 681 191 1471
Fragments arranged by size:
1498 1471 1135 681 219 191 65
SfoI GGC'GCC
Cuts at: 2117 2320 2117
Size: 203 5057
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5260
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5260
SmaI CCC'GGG
Cuts at: 1872 2587 1872
Size: 715 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3364 4265 4527 820
Size: 197 926 1421 901 262 1553
Fragments arranged by size:
1553 1421 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3051 493
Size: 2558 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5260
SphI G_CATG'C
Cuts at: 1132 2618 1132
Size: 1486 3774
SspI AAT'ATT
Cuts at: 5 52 3184 5
Size: 47 3132 2081
Fragments arranged by size:
3132 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5260
StyI C'CwwG_G
Cuts at: 513 1065 2004 2346 2699 513
Size: 552 939 342 353 3074
Fragments arranged by size:
3074 939 552 353 342
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2455 3287 3304 3457 3642 4087 325
Size: 1255 875 832 17 153 185 445 1498
Fragments arranged by size:
1498 1255 875 832 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2220 2265 3506
Size: 274 80 33 51 1443 243 45 1241
Cuts at: 3506 5218 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1241 274 243 138 80 51
45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5220 5252 5220
Size: 32 5228
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5260
XbaI T'CTAG_A
Cuts at: 2596 2596
Size: 5260
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5260
XmaI C'CCGG_G
Cuts at: 1870 2585 1870
Size: 715 4545
XmnI GAAnn'nnTTC
Cuts at: 3389 3389
Size: 5260
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2280 3068 276
Size: 53 83 186 936 746 788 2468
Fragments arranged by size:
2468 936 788 746 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI XcmI