Restriction map for
pFN21AE1648
(Circular) (Six-base) MAPSORT of: pFN21AE1648.seq Check: 6997 from: 1 to: 5197
pFN21AE1648 5197 bp
With 182 enzymes: SgfI *
January 30, 2014 17:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3007 278
Size: 53 83 186 936 1471 2468
Fragments arranged by size:
2468 1471 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2540 1937
Size: 603 4594
Acc65I G'GTAC_C
Cuts at: 2518 2518
Size: 5197
AclI AA'CG_TT
Cuts at: 1563 3324 3697 1563
Size: 1761 373 3063
Fragments arranged by size:
3063 1761 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3260 4644 4912 1632
Size: 1628 1384 268 1917
Fragments arranged by size:
1917 1628 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5000
AflIII A'CryG_T
Cuts at: 1203 4096 5073 1203
Size: 2893 977 1327
Fragments arranged by size:
2893 1327 977
AgeI A'CCGG_T
Cuts at: 4028 4028
Size: 5197
AhdI GACnn_n'nnGTC
Cuts at: 3926 3926
Size: 5197
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5197
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5197
AlwNI CAG_nnn'CTG
Cuts at: 2175 2940 4512 2175
Size: 765 1572 2860
Fragments arranged by size:
2860 1572 765
ApaI G_GGCC'C
Cuts at: 2169 2169
Size: 5197
ApaLI G'TGCA_C
Cuts at: 3254 4410 3254
Size: 1156 4041
ApoI r'AATT_y
Cuts at: 1493 2506 2732 2884 4993 1493
Size: 1013 226 152 2109 1697
Fragments arranged by size:
2109 1697 1013 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3751 160
Size: 872 2719 1606
Fragments arranged by size:
2719 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5197
AvaI C'yCGr_G
Cuts at: 1870 1943 2080 2141 2345 2462 2522 1870
Size: 73 137 61 204 117 60 4545
Fragments arranged by size:
4545 204 137 117 73 61 60
BamHI G'GATC_C
Cuts at: 2527 2894 2527
Size: 367 4830
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2458 2518 3973 618
Size: 325 217 627 671 60 1455 1842
Fragments arranged by size:
1842 1455 671 627 325 217 60
BanII G_rGCy'C
Cuts at: 1391 2169 2346 2469 2478 2516 1391
Size: 778 177 123 9 38 4072
Fragments arranged by size:
4072 778 177 123 38 9
BbeI G_GCGC'C
Cuts at: 2462 2462
Size: 5197
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4226
BbvCI CC'TCA_GC
Cuts at: 2214 2214
Size: 5197
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3388 3422 1903
Size: 34 50 34 1367 34 3678
Fragments arranged by size:
3678 1367 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3089 4299 3089
Size: 1210 3987
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5197
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2085 2145 2265
Size: 107 122 71 631 605 413 60 120
Cuts at: 2265 2358 3808 136
Size: 93 1450 1525
Fragments arranged by size:
1525 1450 631 605 413 122 120 107
93 71 60
BglII A'GATC_T
Cuts at: 5192 5192
Size: 5197
BlpI GC'TnA_GC
Cuts at: 2614 2614
Size: 5197
Bme1580I G_kGCm'C
Cuts at: 1790 2169 2210 3258 4414 1790
Size: 379 41 1048 1156 2573
Fragments arranged by size:
2573 1156 1048 379 41
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5197
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2928 3886 442
Size: 416 53 83 399 24 1511 958 1753
Fragments arranged by size:
1753 1511 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5197
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 3857 1239
Size: 893 1725 2579
Fragments arranged by size:
2579 1725 893
Bpu10I CC'TnA_GC
Cuts at: 2214 2214
Size: 5197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3322 4187 4485 3322
Size: 865 298 4034
Fragments arranged by size:
4034 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3860 882
Size: 617 2361 2219
Fragments arranged by size:
2361 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2988 493
Size: 711 1784 2702
Fragments arranged by size:
2702 1784 711
BsaBI GATnn'nnATC
Cuts at: 5191 5191
Size: 5197
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2459 2490 3004 3386
Size: 53 83 186 936 926 31 514 382
Cuts at: 3386 4021 275
Size: 635 1451
Fragments arranged by size:
1451 936 926 635 514 382 186 83
53 31
BsaWI w'CCGG_w
Cuts at: 2388 3630 4028 4302 4449 4860 2388
Size: 1242 398 274 147 411 2725
Fragments arranged by size:
2725 1242 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2112 2142 1178
Size: 30 165 30 709 30 4233
Fragments arranged by size:
4233 709 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2044 2249 1186
Size: 858 205 4134
Fragments arranged by size:
4134 858 205
BseYI C'CCAG_C
Cuts at: 2168 2191 2481 4400 4896 2168
Size: 23 290 1919 496 2469
Fragments arranged by size:
2469 1919 496 290 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2465 1163
Size: 573 729 3895
Fragments arranged by size:
3895 729 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2389 2421 2647 3408 4436 1540
Size: 275 186 388 32 226 761 1028 2301
Fragments arranged by size:
2301 1028 761 388 275 226 186 32
BsiHKAI G_wGCw'C
Cuts at: 3258 3343 4414 3258
Size: 85 1071 4041
Fragments arranged by size:
4041 1071 85
BsmI GAATG_Cn'
Cuts at: 2708 2801 2708
Size: 93 5104
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2210 2346 2469 2478 2516 3258
Size: 399 379 41 136 123 9 38 742
Cuts at: 3258 3343 4414 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 379 136 123 85
41 38 9
BspEI T'CCGG_A
Cuts at: 4860 4860
Size: 5197
BspHI T'CATG_A
Cuts at: 3086 4816 3086
Size: 1730 3467
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2253 2552 844
Size: 1409 299 3489
Fragments arranged by size:
3489 1409 299
BsrBI CCG'CTC
Cuts at: 1394 1823 2286 3084 1394
Size: 429 463 798 3507
Fragments arranged by size:
3507 798 463 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3692 3866 65
Size: 1183 2444 174 1396
Fragments arranged by size:
2444 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2416 3841 4028 1700
Size: 110 606 1425 187 2869
Fragments arranged by size:
2869 1425 606 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5197
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5197
BssSI C'ACGA_G
Cuts at: 3257 4269 3257
Size: 1012 4185
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2178 794
Size: 1384 3813
BstBI TT'CG_AA
Cuts at: 4001 4001
Size: 5197
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5197
BstXI CCAn_nnnn'nTGG
Cuts at: 2407 2407
Size: 5197
BstYI r'GATC_y
Cuts at: 1972 2527 2894 3279 3296 4748 4834 4846 5192
Size: 555 367 385 17 1452 86 12 346
Cuts at: 5192 1972
Size: 1977
Fragments arranged by size:
1977 1452 555 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4645
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4458
Fragments arranged by size:
4458 683 56
BtsI GCAGTG_nn'
Cuts at: 2713 3507 3527 2713
Size: 794 20 4383
Fragments arranged by size:
4383 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5197
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5162
DraI TTT'AAA
Cuts at: 2501 2501
Size: 5197
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4204 809
Size: 507 2888 1802
Fragments arranged by size:
2888 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2266 2418 2451
Size: 54 1425 50 275 18 436 152 33
Cuts at: 2451 2644 3533 5162 8
Size: 193 889 1629 43
Fragments arranged by size:
1629 1425 889 436 275 193 152 54
50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2418 2644 1537
Size: 275 606 226 4090
Fragments arranged by size:
4090 606 275 226
EarI CTCTTCn'nnn_
Cuts at: 1353 3127 1353
Size: 1774 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3784 4158 4304 387
Size: 1064 138 2195 374 146 1280
Fragments arranged by size:
2195 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 3260 3857 4644 4912 1239
Size: 393 500 1128 597 787 268 1524
Fragments arranged by size:
1524 1128 787 597 500 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2424 2424
Size: 5197
EcoRI G'AATT_C
Cuts at: 2506 2884 2506
Size: 378 4819
FseI GG_CCGG'CC
Cuts at: 2420 2420
Size: 5197
FspI TGC'GCA
Cuts at: 3703 3703
Size: 5197
HaeII r_GCGC'y
Cuts at: 1678 2222 2462 4344 1678
Size: 544 240 1882 2531
Fragments arranged by size:
2531 1882 544 240
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2112 2144 3838 3870 3912
Size: 32 135 32 540 32 1694 32 42
Cuts at: 3912 3944 1373
Size: 32 2626
Fragments arranged by size:
2626 1694 540 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2541 2787 669
Size: 988 281 603 246 3079
Fragments arranged by size:
3079 988 603 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5197
HpaI GTT'AAC
Cuts at: 2787 2787
Size: 5197
KasI G'GCGC_C
Cuts at: 2458 2458
Size: 5197
KpnI G_GTAC'C
Cuts at: 2522 2522
Size: 5197
MfeI C'AATT_G
Cuts at: 2796 2796
Size: 5197
MluI A'CGCG_T
Cuts at: 5073 5073
Size: 5197
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2130 4311 4495 1305
Size: 825 2181 184 2007
Fragments arranged by size:
2181 2007 825 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3375
Fragments arranged by size:
3375 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3155 3514 3673 518
Size: 648 1989 359 159 2042
Fragments arranged by size:
2042 1989 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2157 2295 2407 2449 2613 3290 4438 4683
Size: 551 138 112 42 164 677 1148 245
Cuts at: 4683 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 551 245 164 138 112
42
NaeI GCC'GGC
Cuts at: 2418 2418
Size: 5197
NarI GG'CG_CC
Cuts at: 2459 2459
Size: 5197
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4645
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5197
NgoMIV G'CCGG_C
Cuts at: 2416 2416
Size: 5197
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5197
NotI GC'GGCC_GC
Cuts at: 2644 2644
Size: 5197
NspI r_CATG'y
Cuts at: 1132 1229 2555 4100 1132
Size: 97 1326 1545 2229
Fragments arranged by size:
2229 1545 1326 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5197
PciI A'CATG_T
Cuts at: 4096 4096
Size: 5197
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5197
PmeI GTTT'AAAC
Cuts at: 2501 2501
Size: 5197
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3266 3298 4779 4811 3266
Size: 32 1481 32 3652
Fragments arranged by size:
3652 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5197
PsiI TTA'TAA
Cuts at: 2767 2767
Size: 5197
PspOMI G'GGCC_C
Cuts at: 2165 2165
Size: 5197
PstI C_TGCA'G
Cuts at: 830 2549 830
Size: 1719 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5197
PvuII CAG'CTG
Cuts at: 2407 2449 2407
Size: 42 5155
SalI G'TCGA_C
Cuts at: 1936 2539 1936
Size: 603 4594
SbfI CC_TGCA'GG
Cuts at: 2549 2549
Size: 5197
ScaI AGT'ACT
Cuts at: 3445 5157 3445
Size: 1712 3485
SfcI C'TryA_G
Cuts at: 826 1045 2545 3680 4361 4552 826
Size: 219 1500 1135 681 191 1471
Fragments arranged by size:
1500 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2460 2460
Size: 5197
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5197
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5197
SmaI CCC'GGG
Cuts at: 1872 2143 2524 1872
Size: 271 381 4545
Fragments arranged by size:
4545 381 271
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3301 4202 4464 820
Size: 197 926 1358 901 262 1553
Fragments arranged by size:
1553 1358 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2988 493
Size: 2495 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5197
SphI G_CATG'C
Cuts at: 1132 2555 1132
Size: 1423 3774
SspI AAT'ATT
Cuts at: 5 52 3121 5
Size: 47 3069 2081
Fragments arranged by size:
3069 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5197
StyI C'CwwG_G
Cuts at: 513 1065 2636 513
Size: 552 1571 3074
Fragments arranged by size:
3074 1571 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2354 3224 3241 3394 3579 4024 325
Size: 1255 774 870 17 153 185 445 1498
Fragments arranged by size:
1498 1255 870 774 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3443 5155 96
Size: 274 80 33 51 1443 1466 1712 138
Fragments arranged by size:
1712 1466 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5157 5189 5157
Size: 32 5165
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5197
XbaI T'CTAG_A
Cuts at: 2533 2533
Size: 5197
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2107 2107
Size: 5197
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5197
XmaI C'CCGG_G
Cuts at: 1870 2141 2522 1870
Size: 271 381 4545
Fragments arranged by size:
4545 381 271
XmnI GAAnn'nnTTC
Cuts at: 3326 3326
Size: 5197
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3005 276
Size: 53 83 186 936 1471 2468
Fragments arranged by size:
2468 1471 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FspAI NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI