Restriction map for
pFN21AE1642
(Circular) (Six-base) MAPSORT of: pFN21AE1642.seq Check: 6874 from: 1 to: 5173
pFN21AE1642 5173 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2983 278
Size: 53 83 186 936 1447 2468
Fragments arranged by size:
2468 1447 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2516 1937
Size: 579 4594
Acc65I G'GTAC_C
Cuts at: 2494 2494
Size: 5173
AclI AA'CG_TT
Cuts at: 1563 3300 3673 1563
Size: 1737 373 3063
Fragments arranged by size:
3063 1737 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2328 3236 4620 4888 1632
Size: 696 908 1384 268 1917
Fragments arranged by size:
1917 1384 908 696 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4976
AflIII A'CryG_T
Cuts at: 1203 4072 5049 1203
Size: 2869 977 1327
Fragments arranged by size:
2869 1327 977
AgeI A'CCGG_T
Cuts at: 4004 4004
Size: 5173
AhdI GACnn_n'nnGTC
Cuts at: 3902 3902
Size: 5173
AleI CACnn'nnGTG
Cuts at: 1166 2375 1166
Size: 1209 3964
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5173
AlwNI CAG_nnn'CTG
Cuts at: 2299 2916 4488 2299
Size: 617 1572 2984
Fragments arranged by size:
2984 1572 617
ApaLI G'TGCA_C
Cuts at: 3230 4386 3230
Size: 1156 4017
ApoI r'AATT_y
Cuts at: 1493 2482 2708 2860 4969 1493
Size: 989 226 152 2109 1697
Fragments arranged by size:
2109 1697 989 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3727 160
Size: 872 2695 1606
Fragments arranged by size:
2695 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5173
AvaI C'yCGr_G
Cuts at: 1870 1943 2077 2208 2390 2498 1870
Size: 73 134 131 182 108 4545
Fragments arranged by size:
4545 182 134 131 108 73
BamHI G'GATC_C
Cuts at: 2503 2870 2503
Size: 367 4806
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2154 2175 2220 2323 2365
Size: 325 217 627 367 21 45 103 42
Cuts at: 2365 2494 3949 618
Size: 129 1455 1842
Fragments arranged by size:
1842 1455 627 367 325 217 129 103
45 42 21
BanII G_rGCy'C
Cuts at: 1391 2096 2136 2397 2418 2492 1391
Size: 705 40 261 21 74 4072
Fragments arranged by size:
4072 705 261 74 40 21
BbeI G_GCGC'C
Cuts at: 2158 2224 2327 2369 2158
Size: 66 103 42 4962
Fragments arranged by size:
4962 103 66 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2278 928
Size: 971 379 3823
Fragments arranged by size:
3823 971 379
BbvCI CC'TCA_GC
Cuts at: 2438 2438
Size: 5173
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3364 3398 1903
Size: 34 1427 34 3678
Fragments arranged by size:
3678 1427 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3065 4275 3065
Size: 1210 3963
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5173
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3784 136
Size: 107 122 71 631 605 2112 1525
Fragments arranged by size:
2112 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2106 5168 2106
Size: 3062 2111
BlpI GC'TnA_GC
Cuts at: 2590 2590
Size: 5173
Bme1580I G_kGCm'C
Cuts at: 1790 2178 3234 4390 1790
Size: 388 1056 1156 2573
Fragments arranged by size:
2573 1156 1056 388
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5173
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2904 3862 442
Size: 416 53 83 399 24 1487 958 1753
Fragments arranged by size:
1753 1487 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5173
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2319 2435 3833 1239
Size: 1080 116 1398 2579
Fragments arranged by size:
2579 1398 1080 116
Bpu10I CC'TnA_GC
Cuts at: 2438 2438
Size: 5173
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3298 4163 4461 3298
Size: 865 298 4010
Fragments arranged by size:
4010 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3836 882
Size: 617 2337 2219
Fragments arranged by size:
2337 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2964 493
Size: 711 1760 2702
Fragments arranged by size:
2702 1760 711
BsaBI GATnn'nnATC
Cuts at: 5167 5167
Size: 5173
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2155 2221 2324 2343
Size: 53 83 186 936 622 66 103 19
Cuts at: 2343 2366 2980 3362 3997 275
Size: 23 614 382 635 1451
Fragments arranged by size:
1451 936 635 622 614 382 186 103
83 66 53 23 19
BsaWI w'CCGG_w
Cuts at: 2096 3606 4004 4278 4425 4836 2096
Size: 1510 398 274 147 411 2433
Fragments arranged by size:
2433 1510 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4948
Fragments arranged by size:
4948 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5173
BseYI C'CCAG_C
Cuts at: 2159 2248 4376 4872 2159
Size: 89 2128 496 2460
Fragments arranged by size:
2460 2128 496 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4600
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2466 2623 3384 4412 1540
Size: 275 186 465 157 761 1028 2301
Fragments arranged by size:
2301 1028 761 465 275 186 157
BsiHKAI G_wGCw'C
Cuts at: 3234 3319 4390 3234
Size: 85 1071 4017
Fragments arranged by size:
4017 1071 85
BsmI GAATG_Cn'
Cuts at: 2684 2777 2684
Size: 93 5080
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2136 2178 2397 2418 2492 3234
Size: 399 306 40 42 219 21 74 742
Cuts at: 3234 3319 4390 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 306 219 85 74
42 40 21
BspEI T'CCGG_A
Cuts at: 4836 4836
Size: 5173
BspHI T'CATG_A
Cuts at: 3062 4792 3062
Size: 1730 3443
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2528 844
Size: 1684 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3060 1394
Size: 429 1237 3507
Fragments arranged by size:
3507 1237 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3668 3842 65
Size: 1183 2420 174 1396
Fragments arranged by size:
2420 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2320 3817 4004 1700
Size: 110 510 1497 187 2869
Fragments arranged by size:
2869 1497 510 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5173
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5173
BssSI C'ACGA_G
Cuts at: 3233 4245 3233
Size: 1012 4161
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5173
BstBI TT'CG_AA
Cuts at: 3977 3977
Size: 5173
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5173
BstYI r'GATC_y
Cuts at: 1972 2106 2295 2503 2870 3255 3272 4724 4810
Size: 134 189 208 367 385 17 1452 86
Cuts at: 4810 4822 5168 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 208 189 134
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4621
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4434
Fragments arranged by size:
4434 683 56
BtsI GCAGTG_nn'
Cuts at: 2689 3483 3503 2689
Size: 794 20 4359
Fragments arranged by size:
4359 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5173
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5138
DraI TTT'AAA
Cuts at: 2477 2477
Size: 5173
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4180 809
Size: 507 2864 1802
Fragments arranged by size:
2864 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2463 2620 3509
Size: 54 1425 50 275 18 633 157 889
Cuts at: 3509 5138 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 633 275 157 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2463 2620 1537
Size: 275 651 157 4090
Fragments arranged by size:
4090 651 275 157
EarI CTCTTCn'nnn_
Cuts at: 1353 2304 3103 1353
Size: 951 799 3423
Fragments arranged by size:
3423 951 799
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3760 4134 4280 387
Size: 1064 138 2171 374 146 1280
Fragments arranged by size:
2171 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2319 2328 2435 3236 3833 4620 4888
Size: 393 687 9 107 801 597 787 268
Cuts at: 4888 1239
Size: 1524
Fragments arranged by size:
1524 801 787 687 597 393 268 107
9
EcoO109I rG'GnC_Cy
Cuts at: 2050 2081 2050
Size: 31 5142
EcoRI G'AATT_C
Cuts at: 2482 2860 2482
Size: 378 4795
FspI TGC'GCA
Cuts at: 3679 3679
Size: 5173
HaeII r_GCGC'y
Cuts at: 1678 2158 2166 2224 2327 2369 4320 1678
Size: 480 8 58 103 42 1951 2531
Fragments arranged by size:
2531 1951 480 103 58 42 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3814 3846 3888 3920 1373
Size: 32 135 32 2242 32 42 32 2626
Fragments arranged by size:
2626 2242 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2517 2763 669
Size: 988 281 579 246 3079
Fragments arranged by size:
3079 988 579 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5173
HpaI GTT'AAC
Cuts at: 2763 2763
Size: 5173
KasI G'GCGC_C
Cuts at: 2154 2220 2323 2365 2154
Size: 66 103 42 4962
Fragments arranged by size:
4962 103 66 42
KpnI G_GTAC'C
Cuts at: 2498 2498
Size: 5173
MfeI C'AATT_G
Cuts at: 2772 2772
Size: 5173
MluI A'CGCG_T
Cuts at: 5049 5049
Size: 5173
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4287 4471 1305
Size: 2982 184 2007
Fragments arranged by size:
2982 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3351
Fragments arranged by size:
3351 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2375 3131 3490 3649 518
Size: 648 1209 756 359 159 2042
Fragments arranged by size:
2042 1209 756 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2022 2141 2227 2589 3266 4414 4659 1606
Size: 416 119 86 362 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 416 362 245 119 86
NaeI GCC'GGC
Cuts at: 2322 2322
Size: 5173
NarI GG'CG_CC
Cuts at: 2155 2221 2324 2366 2155
Size: 66 103 42 4962
Fragments arranged by size:
4962 103 66 42
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4621
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5173
NgoMIV G'CCGG_C
Cuts at: 2320 2320
Size: 5173
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5173
NotI GC'GGCC_GC
Cuts at: 2620 2620
Size: 5173
NspI r_CATG'y
Cuts at: 1132 1229 2531 4076 1132
Size: 97 1302 1545 2229
Fragments arranged by size:
2229 1545 1302 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5173
PciI A'CATG_T
Cuts at: 4072 4072
Size: 5173
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5173
PfoI T'CCnGG_A
Cuts at: 2127 2127
Size: 5173
PmeI GTTT'AAAC
Cuts at: 2477 2477
Size: 5173
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3242 3274 4755 4787 3242
Size: 32 1481 32 3628
Fragments arranged by size:
3628 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2081 2081
Size: 5173
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5173
PsiI TTA'TAA
Cuts at: 2743 2743
Size: 5173
PspXI vC'TCGA_Gb
Cuts at: 2208 2390 2208
Size: 182 4991
PstI C_TGCA'G
Cuts at: 830 2049 2525 830
Size: 1219 476 3478
Fragments arranged by size:
3478 1219 476
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5173
PvuII CAG'CTG
Cuts at: 2022 2227 2022
Size: 205 4968
SalI G'TCGA_C
Cuts at: 1936 2515 1936
Size: 579 4594
SanDI GG'GwC_CC
Cuts at: 2081 2081
Size: 5173
SbfI CC_TGCA'GG
Cuts at: 2049 2525 2049
Size: 476 4697
ScaI AGT'ACT
Cuts at: 3421 5133 3421
Size: 1712 3461
SfcI C'TryA_G
Cuts at: 826 1045 2045 2521 3656 4337 4528 826
Size: 219 1000 476 1135 681 191 1471
Fragments arranged by size:
1471 1135 1000 681 476 219 191
SfoI GGC'GCC
Cuts at: 2156 2222 2325 2367 2156
Size: 66 103 42 4962
Fragments arranged by size:
4962 103 66 42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5173
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5173
SmaI CCC'GGG
Cuts at: 1872 2079 2500 1872
Size: 207 421 4545
Fragments arranged by size:
4545 421 207
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2208 2390 3277 4178 4440 820
Size: 197 926 265 182 887 901 262 1553
Fragments arranged by size:
1553 926 901 887 265 262 197 182
SnaBI TAC'GTA
Cuts at: 493 2964 493
Size: 2471 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5173
SphI G_CATG'C
Cuts at: 1132 2531 1132
Size: 1399 3774
SspI AAT'ATT
Cuts at: 5 52 3097 5
Size: 47 3045 2081
Fragments arranged by size:
3045 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5173
StyI C'CwwG_G
Cuts at: 513 1065 2612 513
Size: 552 1547 3074
Fragments arranged by size:
3074 1547 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3200 3217 3370 3555 4000 325
Size: 1255 1620 17 153 185 445 1498
Fragments arranged by size:
1620 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3419 5131 96
Size: 274 80 33 51 1443 1442 1712 138
Fragments arranged by size:
1712 1443 1442 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5133 5165 5133
Size: 32 5141
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5173
XbaI T'CTAG_A
Cuts at: 2509 2509
Size: 5173
XhoI C'TCGA_G
Cuts at: 1943 2208 2390 1943
Size: 265 182 4726
Fragments arranged by size:
4726 265 182
XmaI C'CCGG_G
Cuts at: 1870 2077 2498 1870
Size: 207 421 4545
Fragments arranged by size:
4545 421 207
XmnI GAAnn'nnTTC
Cuts at: 3302 3302
Size: 5173
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2981 276
Size: 53 83 186 936 1447 2468
Fragments arranged by size:
2468 1447 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI
PmlI PspOMI PsrI RsrII SacI SacII SapI SexAI
SfiI SrfI SwaI XcmI