Restriction map for
pFN21AE1640
(Circular) (Six-base) MAPSORT of: pFN21AE1640.seq Check: 1259 from: 1 to: 5170
pFN21AE1640 5170 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2980 278
Size: 53 83 186 936 1444 2468
Fragments arranged by size:
2468 1444 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2513 1937
Size: 576 4594
Acc65I G'GTAC_C
Cuts at: 2491 2491
Size: 5170
AclI AA'CG_TT
Cuts at: 1563 3297 3670 1563
Size: 1734 373 3063
Fragments arranged by size:
3063 1734 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3233 4617 4885 1632
Size: 1601 1384 268 1917
Fragments arranged by size:
1917 1601 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4973
AflIII A'CryG_T
Cuts at: 1203 4069 5046 1203
Size: 2866 977 1327
Fragments arranged by size:
2866 1327 977
AgeI A'CCGG_T
Cuts at: 4001 4001
Size: 5170
AhdI GACnn_n'nnGTC
Cuts at: 3899 3899
Size: 5170
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5170
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5170
AlwNI CAG_nnn'CTG
Cuts at: 2913 4485 2913
Size: 1572 3598
ApaLI G'TGCA_C
Cuts at: 3227 4383 3227
Size: 1156 4014
ApoI r'AATT_y
Cuts at: 1493 2107 2479 2705 2857 4966 1493
Size: 614 372 226 152 2109 1697
Fragments arranged by size:
2109 1697 614 372 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3724 160
Size: 872 2692 1606
Fragments arranged by size:
2692 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5170
AvaI C'yCGr_G
Cuts at: 1870 1943 2495 1870
Size: 73 552 4545
Fragments arranged by size:
4545 552 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2408 2441 2408
Size: 33 5137
BamHI G'GATC_C
Cuts at: 2500 2867 2500
Size: 367 4803
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2491 3946 618
Size: 325 217 627 704 1455 1842
Fragments arranged by size:
1842 1455 704 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2489 1391
Size: 1098 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2024 928
Size: 971 125 4074
Fragments arranged by size:
4074 971 125
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3361 3395 1903
Size: 34 1424 34 3678
Fragments arranged by size:
3678 1424 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3062 4272 3062
Size: 1210 3960
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5170
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2295 3781 136
Size: 107 122 71 631 605 623 1486 1525
Fragments arranged by size:
1525 1486 631 623 605 122 107 71
BglII A'GATC_T
Cuts at: 5165 5165
Size: 5170
BlpI GC'TnA_GC
Cuts at: 2048 2587 2048
Size: 539 4631
Bme1580I G_kGCm'C
Cuts at: 1790 3231 4387 1790
Size: 1441 1156 2573
Fragments arranged by size:
2573 1441 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5170
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2901 3859 442
Size: 416 53 83 399 24 1484 958 1753
Fragments arranged by size:
1753 1484 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5170
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2429 3830 1239
Size: 836 354 1401 2579
Fragments arranged by size:
2579 1401 836 354
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3295 4160 4458 3295
Size: 865 298 4007
Fragments arranged by size:
4007 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3833 882
Size: 617 2334 2219
Fragments arranged by size:
2334 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2961 493
Size: 711 1757 2702
Fragments arranged by size:
2702 1757 711
BsaBI GATnn'nnATC
Cuts at: 5164 5164
Size: 5170
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2977 3359 3994 275
Size: 53 83 186 936 1444 382 635 1451
Fragments arranged by size:
1451 1444 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2163 3603 4001 4275 4422 4833 2163
Size: 1440 398 274 147 411 2500
Fragments arranged by size:
2500 1440 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2267 2297 1178
Size: 30 165 30 864 30 4051
Fragments arranged by size:
4051 864 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5170
BseYI C'CCAG_C
Cuts at: 4373 4869 4373
Size: 496 4674
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2419 1163
Size: 573 683 3914
Fragments arranged by size:
3914 683 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2620 3381 4409 1540
Size: 275 186 619 761 1028 2301
Fragments arranged by size:
2301 1028 761 619 275 186
BsiHKAI G_wGCw'C
Cuts at: 3231 3316 4387 3231
Size: 85 1071 4014
Fragments arranged by size:
4014 1071 85
BsmI GAATG_Cn'
Cuts at: 2681 2774 2681
Size: 93 5077
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2489 3231 3316 4387 1391
Size: 399 699 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 699 399 85
BspEI T'CCGG_A
Cuts at: 4833 4833
Size: 5170
BspHI T'CATG_A
Cuts at: 3059 4789 3059
Size: 1730 3440
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2525 844
Size: 1681 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3057 1394
Size: 429 1234 3507
Fragments arranged by size:
3507 1234 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2245 3665 3839 65
Size: 1183 997 1420 174 1396
Fragments arranged by size:
1420 1396 1183 997 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3814 4001 1700
Size: 110 2004 187 2869
Fragments arranged by size:
2869 2004 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5170
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5170
BssSI C'ACGA_G
Cuts at: 3230 4242 3230
Size: 1012 4158
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5170
BstBI TT'CG_AA
Cuts at: 3974 3974
Size: 5170
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5170
BstYI r'GATC_y
Cuts at: 1972 2358 2500 2867 3252 3269 4721 4807 4819
Size: 386 142 367 385 17 1452 86 12
Cuts at: 4819 5165 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 386 385 367 346 142 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2427 513
Size: 552 1362 3256
Fragments arranged by size:
3256 1362 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4431
Fragments arranged by size:
4431 683 56
BtsI GCAGTG_nn'
Cuts at: 2033 2386 2686 3480 3500 2033
Size: 353 300 794 20 3703
Fragments arranged by size:
3703 794 353 300 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5170
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5135
DraI TTT'AAA
Cuts at: 2474 2474
Size: 5170
DraIII CAC_nnn'GTG
Cuts at: 2464 2464
Size: 5170
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4177 809
Size: 507 2861 1802
Fragments arranged by size:
2861 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2466 2617 3506
Size: 54 1425 50 275 18 636 151 889
Cuts at: 3506 5135 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 636 275 151 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2617 1537
Size: 275 805 4090
Fragments arranged by size:
4090 805 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3100 1353
Size: 1747 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3757 4131 4277 387
Size: 1064 138 2168 374 146 1280
Fragments arranged by size:
2168 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2429 3233 3830 4617 4885 1239
Size: 393 443 354 804 597 787 268 1524
Fragments arranged by size:
1524 804 787 597 443 393 354 268
EcoO109I rG'GnC_Cy
Cuts at: 2190 2245 2190
Size: 55 5115
EcoRI G'AATT_C
Cuts at: 2479 2857 2479
Size: 378 4792
EcoRV GAT'ATC
Cuts at: 2258 2258
Size: 5170
FspI TGC'GCA
Cuts at: 2372 3676 2372
Size: 1304 3866
HaeII r_GCGC'y
Cuts at: 1678 4317 1678
Size: 2639 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3811 3843 3885 3917 1373
Size: 32 135 32 2239 32 42 32 2626
Fragments arranged by size:
2626 2239 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2146 2514 2760 669
Size: 988 281 208 368 246 3079
Fragments arranged by size:
3079 988 368 281 246 208
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5170
HpaI GTT'AAC
Cuts at: 2760 2760
Size: 5170
KpnI G_GTAC'C
Cuts at: 2495 2495
Size: 5170
MfeI C'AATT_G
Cuts at: 2769 2769
Size: 5170
MluI A'CGCG_T
Cuts at: 5046 5046
Size: 5170
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4284 4468 1305
Size: 2979 184 2007
Fragments arranged by size:
2979 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3348
Fragments arranged by size:
3348 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2238 3128 3487 3646 518
Size: 648 1072 890 359 159 2042
Fragments arranged by size:
2042 1072 890 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2586 3263 4411 4656 1606
Size: 980 677 1148 245 2120
Fragments arranged by size:
2120 1148 980 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4618
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5170
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5170
NotI GC'GGCC_GC
Cuts at: 2617 2617
Size: 5170
NspI r_CATG'y
Cuts at: 1132 1229 2528 4073 1132
Size: 97 1299 1545 2229
Fragments arranged by size:
2229 1545 1299 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5170
PciI A'CATG_T
Cuts at: 4069 4069
Size: 5170
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2464 1108
Size: 1356 3814
PfoI T'CCnGG_A
Cuts at: 2095 2095
Size: 5170
PmeI GTTT'AAAC
Cuts at: 2474 2474
Size: 5170
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3239 3271 4752 4784 3239
Size: 32 1481 32 3625
Fragments arranged by size:
3625 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2190 2245 2190
Size: 55 5115
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5170
PsiI TTA'TAA
Cuts at: 2740 2740
Size: 5170
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2309 2341 2309
Size: 32 5138
PstI C_TGCA'G
Cuts at: 830 2522 830
Size: 1692 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5170
SalI G'TCGA_C
Cuts at: 1936 2512 1936
Size: 576 4594
SbfI CC_TGCA'GG
Cuts at: 2522 2522
Size: 5170
ScaI AGT'ACT
Cuts at: 3418 5130 3418
Size: 1712 3458
SfcI C'TryA_G
Cuts at: 826 1045 2518 3653 4334 4525 826
Size: 219 1473 1135 681 191 1471
Fragments arranged by size:
1473 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5170
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5170
SmaI CCC'GGG
Cuts at: 1872 2497 1872
Size: 625 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3274 4175 4437 820
Size: 197 926 1331 901 262 1553
Fragments arranged by size:
1553 1331 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2961 493
Size: 2468 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5170
SphI G_CATG'C
Cuts at: 1132 2528 1132
Size: 1396 3774
SspI AAT'ATT
Cuts at: 5 52 3094 5
Size: 47 3042 2081
Fragments arranged by size:
3042 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5170
StyI C'CwwG_G
Cuts at: 513 1065 2222 2609 513
Size: 552 1157 387 3074
Fragments arranged by size:
3074 1157 552 387
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3197 3214 3367 3552 3997 325
Size: 1255 1617 17 153 185 445 1498
Fragments arranged by size:
1617 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3416 5128 96
Size: 274 80 33 51 1443 1439 1712 138
Fragments arranged by size:
1712 1443 1439 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2267 2299 5130 5162 2267
Size: 32 2831 32 2275
Fragments arranged by size:
2831 2275 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5170
XbaI T'CTAG_A
Cuts at: 2506 2506
Size: 5170
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5170
XmaI C'CCGG_G
Cuts at: 1870 2495 1870
Size: 625 4545
XmnI GAAnn'nnTTC
Cuts at: 3299 3299
Size: 5170
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2978 276
Size: 53 83 186 936 1444 2468
Fragments arranged by size:
2468 1444 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI