Restriction map for
pFN21AE1632
(Circular) (Six-base) MAPSORT of: pFN21AE1632.seq Check: 1212 from: 1 to: 5146
pFN21AE1632 5146 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2956 278
Size: 53 83 186 936 1420 2468
Fragments arranged by size:
2468 1420 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2190 2489 1937
Size: 253 299 4594
Fragments arranged by size:
4594 299 253
Acc65I G'GTAC_C
Cuts at: 2467 2467
Size: 5146
AclI AA'CG_TT
Cuts at: 1563 3273 3646 1563
Size: 1710 373 3063
Fragments arranged by size:
3063 1710 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2037 3209 4593 4861 1632
Size: 405 1172 1384 268 1917
Fragments arranged by size:
1917 1384 1172 405 268
AfeI AGC'GCT
Cuts at: 2090 2090
Size: 5146
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4949
AflIII A'CryG_T
Cuts at: 1203 2431 4045 5022 1203
Size: 1228 1614 977 1327
Fragments arranged by size:
1614 1327 1228 977
AgeI A'CCGG_T
Cuts at: 3977 3977
Size: 5146
AhdI GACnn_n'nnGTC
Cuts at: 3875 3875
Size: 5146
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5146
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5146
AlwNI CAG_nnn'CTG
Cuts at: 2889 4461 2889
Size: 1572 3574
ApaI G_GGCC'C
Cuts at: 2186 2186
Size: 5146
ApaLI G'TGCA_C
Cuts at: 3203 4359 3203
Size: 1156 3990
ApoI r'AATT_y
Cuts at: 1493 2455 2681 2833 4942 1493
Size: 962 226 152 2109 1697
Fragments arranged by size:
2109 1697 962 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3700 160
Size: 872 2668 1606
Fragments arranged by size:
2668 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5146
AvaI C'yCGr_G
Cuts at: 1870 1943 2471 1870
Size: 73 528 4545
Fragments arranged by size:
4545 528 73
BamHI G'GATC_C
Cuts at: 2476 2843 2476
Size: 367 4779
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2398 2405 2467 3922 618
Size: 325 217 627 611 7 62 1455 1842
Fragments arranged by size:
1842 1455 627 611 325 217 62 7
BanII G_rGCy'C
Cuts at: 1391 2186 2465 1391
Size: 795 279 4072
Fragments arranged by size:
4072 795 279
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4175
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3337 3371 1903
Size: 34 1400 34 3678
Fragments arranged by size:
3678 1400 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3038 4248 3038
Size: 1210 3936
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5146
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3757 136
Size: 107 122 71 631 605 2085 1525
Fragments arranged by size:
2085 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5141 5141
Size: 5146
BlpI GC'TnA_GC
Cuts at: 2563 2563
Size: 5146
Bme1580I G_kGCm'C
Cuts at: 1790 2186 3207 4363 1790
Size: 396 1021 1156 2573
Fragments arranged by size:
2573 1156 1021 396
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5146
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2300 2877 3835
Size: 416 53 83 399 24 883 577 958
Cuts at: 3835 442
Size: 1753
Fragments arranged by size:
1753 958 883 577 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5146
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3806 1239
Size: 2567 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3271 4136 4434 3271
Size: 865 298 3983
Fragments arranged by size:
3983 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2411 3809 882
Size: 617 912 1398 2219
Fragments arranged by size:
2219 1398 912 617
BsaAI yAC'GTr
Cuts at: 493 1204 2937 493
Size: 711 1733 2702
Fragments arranged by size:
2702 1733 711
BsaBI GATnn'nnATC
Cuts at: 5140 5140
Size: 5146
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2953 3335 3970 275
Size: 53 83 186 936 1420 382 635 1451
Fragments arranged by size:
1451 1420 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3579 3977 4251 4398 4809 3579
Size: 398 274 147 411 3916
Fragments arranged by size:
3916 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4921
Fragments arranged by size:
4921 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5146
BseYI C'CCAG_C
Cuts at: 2245 4349 4845 2245
Size: 2104 496 2546
Fragments arranged by size:
2546 2104 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2258 1163
Size: 573 522 4051
Fragments arranged by size:
4051 573 522
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2596 3357 4385 1540
Size: 275 186 595 761 1028 2301
Fragments arranged by size:
2301 1028 761 595 275 186
BsiHKAI G_wGCw'C
Cuts at: 2342 3207 3292 4363 2342
Size: 865 85 1071 3125
Fragments arranged by size:
3125 1071 865 85
BsmI GAATG_Cn'
Cuts at: 2657 2750 2657
Size: 93 5053
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2186 2342 2465 3207 3292 4363 1391
Size: 399 396 156 123 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 396 156 123 85
BspEI T'CCGG_A
Cuts at: 4809 4809
Size: 5146
BspHI T'CATG_A
Cuts at: 3035 4765 3035
Size: 1730 3416
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2501 844
Size: 1657 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3033 1394
Size: 429 1210 3507
Fragments arranged by size:
3507 1210 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3641 3815 65
Size: 1183 2393 174 1396
Fragments arranged by size:
2393 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2217 3790 3977 1700
Size: 110 407 1573 187 2869
Fragments arranged by size:
2869 1573 407 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5146
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5146
BssSI C'ACGA_G
Cuts at: 3206 4218 3206
Size: 1012 4134
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5146
BstBI TT'CG_AA
Cuts at: 3950 3950
Size: 5146
BstEII G'GTnAC_C
Cuts at: 1184 2323 1184
Size: 1139 4007
BstYI r'GATC_y
Cuts at: 1972 2476 2843 3228 3245 4697 4783 4795 5141
Size: 504 367 385 17 1452 86 12 346
Cuts at: 5141 1972
Size: 1977
Fragments arranged by size:
1977 1452 504 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2230 2373 513
Size: 552 1165 143 3286
Fragments arranged by size:
3286 1165 552 143
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4407
Fragments arranged by size:
4407 683 56
BtsI GCAGTG_nn'
Cuts at: 2129 2662 3456 3476 2129
Size: 533 794 20 3799
Fragments arranged by size:
3799 794 533 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5146
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5111
DraI TTT'AAA
Cuts at: 2450 2450
Size: 5146
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4153 809
Size: 507 2837 1802
Fragments arranged by size:
2837 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2593 3482 5111
Size: 54 1425 50 275 18 763 889 1629
Cuts at: 5111 8
Size: 43
Fragments arranged by size:
1629 1425 889 763 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2593 1537
Size: 275 781 4090
Fragments arranged by size:
4090 781 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3076 1353
Size: 1723 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3733 4107 4253 387
Size: 1064 138 2144 374 146 1280
Fragments arranged by size:
2144 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2037 3209 3806 4593 4861 1239
Size: 393 405 1172 597 787 268 1524
Fragments arranged by size:
1524 1172 787 597 405 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2151 2182 2183 2151
Size: 31 1 5114
Fragments arranged by size:
5114 31 1
EcoRI G'AATT_C
Cuts at: 2455 2833 2455
Size: 378 4768
FspI TGC'GCA
Cuts at: 3652 3652
Size: 5146
HaeII r_GCGC'y
Cuts at: 1678 2092 4293 1678
Size: 414 2201 2531
Fragments arranged by size:
2531 2201 414
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2408 2421 2440 2453 3787
Size: 32 135 32 836 13 19 13 1334
Cuts at: 3787 3819 3861 3893 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1334 836 135 42 32 32 32
32 19 13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2490 2736 669
Size: 988 281 552 246 3079
Fragments arranged by size:
3079 988 552 281 246
HindIII A'AGCT_T
Cuts at: 748 2049 748
Size: 1301 3845
HpaI GTT'AAC
Cuts at: 2736 2736
Size: 5146
KpnI G_GTAC'C
Cuts at: 2471 2471
Size: 5146
MfeI C'AATT_G
Cuts at: 2745 2745
Size: 5146
MluI A'CGCG_T
Cuts at: 5022 5022
Size: 5146
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4260 4444 1305
Size: 2955 184 2007
Fragments arranged by size:
2955 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3324
Fragments arranged by size:
3324 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3104 3463 3622 518
Size: 648 1938 359 159 2042
Fragments arranged by size:
2042 1938 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2249 2562 3239 4387 4632 1606
Size: 643 313 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 643 313 245
NcoI C'CATG_G
Cuts at: 513 1065 2230 513
Size: 552 1165 3429
Fragments arranged by size:
3429 1165 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5146
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5146
NotI GC'GGCC_GC
Cuts at: 2593 2593
Size: 5146
NspI r_CATG'y
Cuts at: 1132 1229 2435 2504 4049 1132
Size: 97 1206 69 1545 2229
Fragments arranged by size:
2229 1545 1206 97 69
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5146
PciI A'CATG_T
Cuts at: 2431 4045 2431
Size: 1614 3532
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2252 1108
Size: 1144 4002
PmeI GTTT'AAAC
Cuts at: 2450 2450
Size: 5146
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3215 3247 4728 4760 3215
Size: 32 1481 32 3601
Fragments arranged by size:
3601 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2151 2151
Size: 5146
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5146
PsiI TTA'TAA
Cuts at: 2716 2716
Size: 5146
PspOMI G'GGCC_C
Cuts at: 2182 2182
Size: 5146
PstI C_TGCA'G
Cuts at: 830 2370 2498 830
Size: 1540 128 3478
Fragments arranged by size:
3478 1540 128
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5146
PvuII CAG'CTG
Cuts at: 2249 2249
Size: 5146
SalI G'TCGA_C
Cuts at: 1936 2488 1936
Size: 552 4594
SbfI CC_TGCA'GG
Cuts at: 2498 2498
Size: 5146
ScaI AGT'ACT
Cuts at: 3394 5106 3394
Size: 1712 3434
SexAI A'CCwGG_T
Cuts at: 2420 2420
Size: 5146
SfcI C'TryA_G
Cuts at: 826 1045 2366 2494 3629 4310 4501 826
Size: 219 1321 128 1135 681 191 1471
Fragments arranged by size:
1471 1321 1135 681 219 191 128
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5146
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5146
SmaI CCC'GGG
Cuts at: 1872 2473 1872
Size: 601 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3250 4151 4413 820
Size: 197 926 1307 901 262 1553
Fragments arranged by size:
1553 1307 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2937 493
Size: 2444 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5146
SphI G_CATG'C
Cuts at: 1132 2504 1132
Size: 1372 3774
SspI AAT'ATT
Cuts at: 5 52 3070 5
Size: 47 3018 2081
Fragments arranged by size:
3018 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5146
StyI C'CwwG_G
Cuts at: 513 1065 2223 2230 2585 513
Size: 552 1158 7 355 3074
Fragments arranged by size:
3074 1158 552 355 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2057 2365 3173 3190 3343 3528 3973
Size: 1255 477 308 808 17 153 185 445
Cuts at: 3973 325
Size: 1498
Fragments arranged by size:
1498 1255 808 477 445 308 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3392 5104 96
Size: 274 80 33 51 1443 1415 1712 138
Fragments arranged by size:
1712 1443 1415 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5106 5138 5106
Size: 32 5114
Tth111I GACn'n_nGTC
Cuts at: 1316 2321 2422 1316
Size: 1005 101 4040
Fragments arranged by size:
4040 1005 101
XbaI T'CTAG_A
Cuts at: 2482 2482
Size: 5146
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5146
XmaI C'CCGG_G
Cuts at: 1870 2471 1870
Size: 601 4545
XmnI GAAnn'nnTTC
Cuts at: 3275 3275
Size: 5146
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2954 276
Size: 53 83 186 936 1420 2468
Fragments arranged by size:
2468 1420 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI XcmI