Restriction map for
pFN21AE1619
(Circular) (Six-base) MAPSORT of: pFN21AE1619.seq Check: 2652 from: 1 to: 5101
pFN21AE1619 5101 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2287 2287
Size: 5101
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2911 278
Size: 53 83 186 936 1375 2468
Fragments arranged by size:
2468 1375 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2444 1937
Size: 507 4594
Acc65I G'GTAC_C
Cuts at: 2422 2422
Size: 5101
AclI AA'CG_TT
Cuts at: 1563 3228 3601 1563
Size: 1665 373 3063
Fragments arranged by size:
3063 1665 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2177 3164 4548 4816 1632
Size: 545 987 1384 268 1917
Fragments arranged by size:
1917 1384 987 545 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4904
AflIII A'CryG_T
Cuts at: 1203 4000 4977 1203
Size: 2797 977 1327
Fragments arranged by size:
2797 1327 977
AgeI A'CCGG_T
Cuts at: 3932 3932
Size: 5101
AhdI GACnn_n'nnGTC
Cuts at: 3830 3830
Size: 5101
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5101
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5101
AlwNI CAG_nnn'CTG
Cuts at: 2116 2844 4416 2116
Size: 728 1572 2801
Fragments arranged by size:
2801 1572 728
ApaI G_GGCC'C
Cuts at: 2152 2152
Size: 5101
ApaLI G'TGCA_C
Cuts at: 3158 4314 3158
Size: 1156 3945
ApoI r'AATT_y
Cuts at: 1493 2410 2636 2788 4897 1493
Size: 917 226 152 2109 1697
Fragments arranged by size:
2109 1697 917 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3655 160
Size: 872 2623 1606
Fragments arranged by size:
2623 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5101
AvaI C'yCGr_G
Cuts at: 1870 1943 2426 1870
Size: 73 483 4545
Fragments arranged by size:
4545 483 73
BamHI G'GATC_C
Cuts at: 2431 2798 2431
Size: 367 4734
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2299 2422 3877 618
Size: 325 217 627 512 123 1455 1842
Fragments arranged by size:
1842 1455 627 512 325 217 123
BanII G_rGCy'C
Cuts at: 1391 2068 2130 2152 2247 2420 1391
Size: 677 62 22 95 173 4072
Fragments arranged by size:
4072 677 173 95 62 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2168 928
Size: 971 269 3861
Fragments arranged by size:
3861 971 269
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3292 3326 1903
Size: 34 50 34 1271 34 3678
Fragments arranged by size:
3678 1271 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2993 4203 2993
Size: 1210 3891
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5101
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3712 136
Size: 107 122 71 631 605 2040 1525
Fragments arranged by size:
2040 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5096 5096
Size: 5101
BlpI GC'TnA_GC
Cuts at: 2024 2518 2024
Size: 494 4607
Bme1580I G_kGCm'C
Cuts at: 1790 2035 2152 3162 4318 1790
Size: 245 117 1010 1156 2573
Fragments arranged by size:
2573 1156 1010 245 117
BmgBI CAC'GTC
Cuts at: 1318 2258 1318
Size: 940 4161
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2832 3790 442
Size: 416 53 83 399 24 1415 958 1753
Fragments arranged by size:
1753 1415 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5101
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2266 2266
Size: 5101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3761 1239
Size: 2522 2579
Bpu10I CC'TnA_GC
Cuts at: 2205 2205
Size: 5101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3226 4091 4389 3226
Size: 865 298 3938
Fragments arranged by size:
3938 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3764 882
Size: 617 2265 2219
Fragments arranged by size:
2265 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2325 2892 493
Size: 711 1121 567 2702
Fragments arranged by size:
2702 1121 711 567
BsaBI GATnn'nnATC
Cuts at: 5095 5095
Size: 5101
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2908 3290 3925 275
Size: 53 83 186 936 1375 382 635 1451
Fragments arranged by size:
1451 1375 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3534 3932 4206 4353 4764 3534
Size: 398 274 147 411 3871
Fragments arranged by size:
3871 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4876
Fragments arranged by size:
4876 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2076 2242 1186
Size: 890 166 4045
Fragments arranged by size:
4045 890 166
BseYI C'CCAG_C
Cuts at: 2368 4304 4800 2368
Size: 1936 496 2669
Fragments arranged by size:
2669 1936 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4528
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2551 3312 4340 1540
Size: 275 186 550 761 1028 2301
Fragments arranged by size:
2301 1028 761 550 275 186
BsiHKAI G_wGCw'C
Cuts at: 2130 3162 3247 4318 2130
Size: 1032 85 1071 2913
Fragments arranged by size:
2913 1071 1032 85
BsmI GAATG_Cn'
Cuts at: 2612 2705 2612
Size: 93 5008
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2068 2130 2152 2247 2420 3162
Size: 399 245 33 62 22 95 173 742
Cuts at: 3162 3247 4318 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 245 173 95 85
62 33 22
BspEI T'CCGG_A
Cuts at: 4764 4764
Size: 5101
BspHI T'CATG_A
Cuts at: 2990 4720 2990
Size: 1730 3371
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2238 2287 2456 844
Size: 1394 49 169 3489
Fragments arranged by size:
3489 1394 169 49
BsrBI CCG'CTC
Cuts at: 1394 1823 2250 2988 1394
Size: 429 427 738 3507
Fragments arranged by size:
3507 738 429 427
BsrDI GCAATG_nn'
Cuts at: 65 1248 3596 3770 65
Size: 1183 2348 174 1396
Fragments arranged by size:
2348 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3745 3932 1700
Size: 110 1935 187 2869
Fragments arranged by size:
2869 1935 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5101
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5101
BssSI C'ACGA_G
Cuts at: 3161 4173 3161
Size: 1012 4089
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2051 794
Size: 1257 3844
BstBI TT'CG_AA
Cuts at: 3905 3905
Size: 5101
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5101
BstYI r'GATC_y
Cuts at: 1972 2188 2431 2798 3183 3200 4652 4738 4750
Size: 216 243 367 385 17 1452 86 12
Cuts at: 4750 5096 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 243 216 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2085 513
Size: 552 1020 3529
Fragments arranged by size:
3529 1020 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4362
Fragments arranged by size:
4362 683 56
BtsI GCAGTG_nn'
Cuts at: 2045 2617 3411 3431 2045
Size: 572 794 20 3715
Fragments arranged by size:
3715 794 572 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5101
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5066
DraI TTT'AAA
Cuts at: 2405 2405
Size: 5101
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4108 809
Size: 507 2792 1802
Fragments arranged by size:
2792 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2548 3437 5066
Size: 54 1425 50 275 18 718 889 1629
Cuts at: 5066 8
Size: 43
Fragments arranged by size:
1629 1425 889 718 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2548 1537
Size: 275 736 4090
Fragments arranged by size:
4090 736 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2178 3031 1353
Size: 825 853 3423
Fragments arranged by size:
3423 853 825
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3688 4062 4208 387
Size: 1064 138 2099 374 146 1280
Fragments arranged by size:
2099 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2128 2128
Size: 5101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2177 3164 3761 4548 4816 1239
Size: 393 545 987 597 787 268 1524
Fragments arranged by size:
1524 987 787 597 545 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2113 2149 2113
Size: 36 5065
EcoRI G'AATT_C
Cuts at: 2410 2788 2410
Size: 378 4723
FspI TGC'GCA
Cuts at: 3607 3607
Size: 5101
HaeII r_GCGC'y
Cuts at: 1678 4248 1678
Size: 2570 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3742 3774 3816 3848 1373
Size: 32 135 32 2170 32 42 32 2626
Fragments arranged by size:
2626 2170 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2445 2691 669
Size: 988 281 507 246 3079
Fragments arranged by size:
3079 988 507 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5101
HpaI GTT'AAC
Cuts at: 2691 2691
Size: 5101
KpnI G_GTAC'C
Cuts at: 2426 2426
Size: 5101
MfeI C'AATT_G
Cuts at: 2700 2700
Size: 5101
MluI A'CGCG_T
Cuts at: 4977 4977
Size: 5101
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4215 4399 1305
Size: 2910 184 2007
Fragments arranged by size:
2910 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3279
Fragments arranged by size:
3279 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3059 3418 3577 518
Size: 648 1893 359 159 2042
Fragments arranged by size:
2042 1893 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2018 2517 3194 4342 4587 1606
Size: 412 499 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 499 412 245
NcoI C'CATG_G
Cuts at: 513 1065 2085 513
Size: 552 1020 3529
Fragments arranged by size:
3529 1020 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5101
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5101
NotI GC'GGCC_GC
Cuts at: 2548 2548
Size: 5101
NspI r_CATG'y
Cuts at: 1132 1229 2459 4004 1132
Size: 97 1230 1545 2229
Fragments arranged by size:
2229 1545 1230 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5101
PciI A'CATG_T
Cuts at: 4000 4000
Size: 5101
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5101
PmeI GTTT'AAAC
Cuts at: 2405 2405
Size: 5101
PmlI CAC'GTG
Cuts at: 2325 2325
Size: 5101
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3170 3202 4683 4715 3170
Size: 32 1481 32 3556
Fragments arranged by size:
3556 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5101
PsiI TTA'TAA
Cuts at: 2671 2671
Size: 5101
PspOMI G'GGCC_C
Cuts at: 2148 2148
Size: 5101
PstI C_TGCA'G
Cuts at: 830 2173 2235 2393 2453 830
Size: 1343 62 158 60 3478
Fragments arranged by size:
3478 1343 158 62 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5101
SacI G_AGCT'C
Cuts at: 2130 2130
Size: 5101
SalI G'TCGA_C
Cuts at: 1936 2443 1936
Size: 507 4594
SbfI CC_TGCA'GG
Cuts at: 2235 2453 2235
Size: 218 4883
ScaI AGT'ACT
Cuts at: 3349 5061 3349
Size: 1712 3389
SfcI C'TryA_G
Cuts at: 826 1045 2169 2231 2389 2449 3584 4265 4456
Size: 219 1124 62 158 60 1135 681 191
Cuts at: 4456 826
Size: 1471
Fragments arranged by size:
1471 1135 1124 681 219 191 158 62
60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5101
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5101
SmaI CCC'GGG
Cuts at: 1872 2428 1872
Size: 556 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3205 4106 4368 820
Size: 197 926 1262 901 262 1553
Fragments arranged by size:
1553 1262 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2892 493
Size: 2399 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5101
SphI G_CATG'C
Cuts at: 1132 2459 1132
Size: 1327 3774
SspI AAT'ATT
Cuts at: 5 52 3025 5
Size: 47 2973 2081
Fragments arranged by size:
2973 2081 47
StuI AGG'CCT
Cuts at: 1517 2104 2136 2210 1517
Size: 587 32 74 4408
Fragments arranged by size:
4408 587 74 32
StyI C'CwwG_G
Cuts at: 513 1065 2085 2211 2540 513
Size: 552 1020 126 329 3074
Fragments arranged by size:
3074 1020 552 329 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3128 3145 3298 3483 3928 325
Size: 1255 1548 17 153 185 445 1498
Fragments arranged by size:
1548 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3347 5059 96
Size: 274 80 33 51 1443 1370 1712 138
Fragments arranged by size:
1712 1443 1370 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2283 2315 5061 5093 2283
Size: 32 2746 32 2291
Fragments arranged by size:
2746 2291 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5101
XbaI T'CTAG_A
Cuts at: 2437 2437
Size: 5101
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5101
XmaI C'CCGG_G
Cuts at: 1870 2426 1870
Size: 556 4545
XmnI GAAnn'nnTTC
Cuts at: 3230 3230
Size: 5101
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2909 276
Size: 53 83 186 936 1375 2468
Fragments arranged by size:
2468 1375 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PpuMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI