Restriction map for
pFN21AE1617
(Circular) (Six-base) MAPSORT of: pFN21AE1617.seq Check: 4579 from: 1 to: 5086
pFN21AE1617 5086 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2896 278
Size: 53 83 186 936 1360 2468
Fragments arranged by size:
2468 1360 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2429 1937
Size: 492 4594
Acc65I G'GTAC_C
Cuts at: 2407 2407
Size: 5086
AclI AA'CG_TT
Cuts at: 1563 3213 3586 1563
Size: 1650 373 3063
Fragments arranged by size:
3063 1650 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3149 4533 4801 1632
Size: 1517 1384 268 1917
Fragments arranged by size:
1917 1517 1384 268
AfeI AGC'GCT
Cuts at: 2267 2267
Size: 5086
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4889
AflIII A'CryG_T
Cuts at: 1203 3985 4962 1203
Size: 2782 977 1327
Fragments arranged by size:
2782 1327 977
AgeI A'CCGG_T
Cuts at: 3917 3917
Size: 5086
AhdI GACnn_n'nnGTC
Cuts at: 3815 3815
Size: 5086
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5086
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5086
AlwNI CAG_nnn'CTG
Cuts at: 2829 4401 2829
Size: 1572 3514
ApaLI G'TGCA_C
Cuts at: 3143 4299 3143
Size: 1156 3930
ApoI r'AATT_y
Cuts at: 1493 2197 2395 2621 2773 4882 1493
Size: 704 198 226 152 2109 1697
Fragments arranged by size:
2109 1697 704 226 198 152
AseI AT'TA_AT
Cuts at: 160 1032 3640 160
Size: 872 2608 1606
Fragments arranged by size:
2608 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5086
AvaI C'yCGr_G
Cuts at: 1870 1943 2411 1870
Size: 73 468 4545
Fragments arranged by size:
4545 468 73
BamHI G'GATC_C
Cuts at: 2416 2783 2416
Size: 367 4719
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2351 2407 3862 618
Size: 325 217 627 564 56 1455 1842
Fragments arranged by size:
1842 1455 627 564 325 217 56
BanII G_rGCy'C
Cuts at: 1391 2405 1391
Size: 1014 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3277 3311 1903
Size: 34 50 34 1256 34 3678
Fragments arranged by size:
3678 1256 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2978 4188 2978
Size: 1210 3876
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5086
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3697 136
Size: 107 122 71 631 605 2025 1525
Fragments arranged by size:
2025 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5081 5081
Size: 5086
BlpI GC'TnA_GC
Cuts at: 2503 2503
Size: 5086
Bme1580I G_kGCm'C
Cuts at: 1790 2354 3147 4303 1790
Size: 564 793 1156 2573
Fragments arranged by size:
2573 1156 793 564
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5086
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2817 3775 442
Size: 416 53 83 399 24 1400 958 1753
Fragments arranged by size:
1753 1400 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5086
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3746 1239
Size: 2507 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3211 4076 4374 3211
Size: 865 298 3923
Fragments arranged by size:
3923 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3749 882
Size: 617 2250 2219
Fragments arranged by size:
2250 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2877 493
Size: 711 1673 2702
Fragments arranged by size:
2702 1673 711
BsaBI GATnn'nnATC
Cuts at: 5080 5080
Size: 5086
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2893 3275 3910 275
Size: 53 83 186 936 1360 382 635 1451
Fragments arranged by size:
1451 1360 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2158 3519 3917 4191 4338 4749 2158
Size: 1361 398 274 147 411 2495
Fragments arranged by size:
2495 1361 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2363 2393 1178
Size: 30 165 30 960 30 3871
Fragments arranged by size:
3871 960 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5086
BseYI C'CCAG_C
Cuts at: 4289 4785 4289
Size: 496 4590
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4513
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2536 3297 4325 1540
Size: 275 186 535 761 1028 2301
Fragments arranged by size:
2301 1028 761 535 275 186
BsiHKAI G_wGCw'C
Cuts at: 3147 3232 4303 3147
Size: 85 1071 3930
Fragments arranged by size:
3930 1071 85
BsmI GAATG_Cn'
Cuts at: 2597 2690 2597
Size: 93 4993
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2354 2405 3147 3232 4303 1391
Size: 399 564 51 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 564 399 85 51
BspEI T'CCGG_A
Cuts at: 2158 4749 2158
Size: 2591 2495
BspHI T'CATG_A
Cuts at: 2975 4705 2975
Size: 1730 3356
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2140 2441 844
Size: 1296 301 3489
Fragments arranged by size:
3489 1296 301
BsrBI CCG'CTC
Cuts at: 1394 1823 2973 1394
Size: 429 1150 3507
Fragments arranged by size:
3507 1150 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3581 3755 65
Size: 1183 2333 174 1396
Fragments arranged by size:
2333 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3730 3917 1700
Size: 110 1920 187 2869
Fragments arranged by size:
2869 1920 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5086
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5086
BssSI C'ACGA_G
Cuts at: 3146 4158 3146
Size: 1012 4074
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5086
BstBI TT'CG_AA
Cuts at: 2177 3890 2177
Size: 1713 3373
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5086
BstYI r'GATC_y
Cuts at: 1972 2416 2783 3168 3185 4637 4723 4735 5081
Size: 444 367 385 17 1452 86 12 346
Cuts at: 5081 1972
Size: 1977
Fragments arranged by size:
1977 1452 444 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4534
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4347
Fragments arranged by size:
4347 683 56
BtsI GCAGTG_nn'
Cuts at: 2307 2602 3396 3416 2307
Size: 295 794 20 3977
Fragments arranged by size:
3977 794 295 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5086
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5051
DraI TTT'AAA
Cuts at: 2390 2390
Size: 5086
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4093 809
Size: 507 2777 1802
Fragments arranged by size:
2777 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2533 3422 5051
Size: 54 1425 50 275 18 703 889 1629
Cuts at: 5051 8
Size: 43
Fragments arranged by size:
1629 1425 889 703 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2533 1537
Size: 275 721 4090
Fragments arranged by size:
4090 721 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2179 3016 1353
Size: 826 837 3423
Fragments arranged by size:
3423 837 826
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3673 4047 4193 387
Size: 1064 138 2084 374 146 1280
Fragments arranged by size:
2084 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3149 3746 4533 4801 1239
Size: 393 1517 597 787 268 1524
Fragments arranged by size:
1524 1517 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2103 2103
Size: 5086
EcoRI G'AATT_C
Cuts at: 2197 2395 2773 2197
Size: 198 378 4510
Fragments arranged by size:
4510 378 198
EcoRV GAT'ATC
Cuts at: 2215 2215
Size: 5086
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2065 2065
Size: 5086
FspI TGC'GCA
Cuts at: 3592 3592
Size: 5086
HaeII r_GCGC'y
Cuts at: 1678 2269 4233 1678
Size: 591 1964 2531
Fragments arranged by size:
2531 1964 591
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2035 3727 3759 3801
Size: 32 135 32 431 32 1692 32 42
Cuts at: 3801 3833 1373
Size: 32 2626
Fragments arranged by size:
2626 1692 431 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2430 2676 669
Size: 988 281 492 246 3079
Fragments arranged by size:
3079 988 492 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5086
HpaI GTT'AAC
Cuts at: 2676 2676
Size: 5086
KpnI G_GTAC'C
Cuts at: 2411 2411
Size: 5086
MfeI C'AATT_G
Cuts at: 2685 2685
Size: 5086
MluI A'CGCG_T
Cuts at: 4962 4962
Size: 5086
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4200 4384 1305
Size: 2895 184 2007
Fragments arranged by size:
2895 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3264
Fragments arranged by size:
3264 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3044 3403 3562 518
Size: 648 1878 359 159 2042
Fragments arranged by size:
2042 1878 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2502 3179 4327 4572 1606
Size: 896 677 1148 245 2120
Fragments arranged by size:
2120 1148 896 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4534
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5086
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5086
NotI GC'GGCC_GC
Cuts at: 2533 2533
Size: 5086
NspI r_CATG'y
Cuts at: 1132 1229 2339 2444 3989 1132
Size: 97 1110 105 1545 2229
Fragments arranged by size:
2229 1545 1110 105 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5086
PciI A'CATG_T
Cuts at: 3985 3985
Size: 5086
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5086
PmeI GTTT'AAAC
Cuts at: 2390 2390
Size: 5086
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3155 3187 4668 4700 3155
Size: 32 1481 32 3541
Fragments arranged by size:
3541 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2103 2103
Size: 5086
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5086
PsiI TTA'TAA
Cuts at: 2656 2656
Size: 5086
PstI C_TGCA'G
Cuts at: 830 2438 830
Size: 1608 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5086
SalI G'TCGA_C
Cuts at: 1936 2428 1936
Size: 492 4594
SbfI CC_TGCA'GG
Cuts at: 2438 2438
Size: 5086
ScaI AGT'ACT
Cuts at: 3334 5046 3334
Size: 1712 3374
SfcI C'TryA_G
Cuts at: 826 1045 2257 2434 3569 4250 4441 826
Size: 219 1212 177 1135 681 191 1471
Fragments arranged by size:
1471 1212 1135 681 219 191 177
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5086
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5086
SmaI CCC'GGG
Cuts at: 1872 2413 1872
Size: 541 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3190 4091 4353 820
Size: 197 926 1247 901 262 1553
Fragments arranged by size:
1553 1247 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2877 493
Size: 2384 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5086
SphI G_CATG'C
Cuts at: 1132 2444 1132
Size: 1312 3774
SspI AAT'ATT
Cuts at: 5 52 3010 5
Size: 47 2958 2081
Fragments arranged by size:
2958 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5086
StyI C'CwwG_G
Cuts at: 513 1065 2355 2382 2525 513
Size: 552 1290 27 143 3074
Fragments arranged by size:
3074 1290 552 143 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3113 3130 3283 3468 3913 325
Size: 1255 1533 17 153 185 445 1498
Fragments arranged by size:
1533 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2370 3332 5044
Size: 274 80 33 51 1443 393 962 1712
Cuts at: 5044 96
Size: 138
Fragments arranged by size:
1712 1443 962 393 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5046 5078 5046
Size: 32 5054
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5086
XbaI T'CTAG_A
Cuts at: 2422 2422
Size: 5086
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2162 2162
Size: 5086
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5086
XmaI C'CCGG_G
Cuts at: 1870 2411 1870
Size: 541 4545
XmnI GAAnn'nnTTC
Cuts at: 2201 3215 2201
Size: 1014 4072
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2894 276
Size: 53 83 186 936 1360 2468
Fragments arranged by size:
2468 1360 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI