Restriction map for
pFN21AE1600
(Circular) (Six-base) MAPSORT of: pFN21AE1600.seq Check: 1959 from: 1 to: 11398
pFN21AE1600 11398 bp
With 182 enzymes: SgfI *
July 13, 2010 13:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7590 9208 278
Size: 53 83 186 936 6054 1618 2468
Fragments arranged by size:
6054 2468 1618 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5169 8526 8741 1937
Size: 3232 3357 215 4594
Fragments arranged by size:
4594 3357 3232 215
Acc65I G'GTAC_C
Cuts at: 2697 3702 6451 8719 2697
Size: 1005 2749 2268 5376
Fragments arranged by size:
5376 2749 2268 1005
AclI AA'CG_TT
Cuts at: 1563 5340 9525 9898 1563
Size: 3777 4185 373 3063
Fragments arranged by size:
4185 3777 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5434 5600 7566 7638 9461 10845 11113 1632
Size: 3802 166 1966 72 1823 1384 268 1917
Fragments arranged by size:
3802 1966 1917 1823 1384 268 166 72
AfeI AGC'GCT
Cuts at: 2539 3041 6632 2539
Size: 502 3591 7305
Fragments arranged by size:
7305 3591 502
AflII C'TTAA_G
Cuts at: 820 1017 7498 820
Size: 197 6481 4720
Fragments arranged by size:
6481 4720 197
AflIII A'CryG_T
Cuts at: 1203 3720 5874 6120 8019 10297 11274 1203
Size: 2517 2154 246 1899 2278 977 1327
Fragments arranged by size:
2517 2278 2154 1899 1327 977 246
AgeI A'CCGG_T
Cuts at: 10229 10229
Size: 11398
AhdI GACnn_n'nnGTC
Cuts at: 4311 5261 7114 10127 4311
Size: 950 1853 3013 5582
Fragments arranged by size:
5582 3013 1853 950
AleI CACnn'nnGTG
Cuts at: 1166 5126 1166
Size: 3960 7438
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4665 5762 6377 8102 1184
Size: 3481 1097 615 1725 4480
Fragments arranged by size:
4480 3481 1725 1097 615
AlwNI CAG_nnn'CTG
Cuts at: 3679 4820 5395 9141 10713 3679
Size: 1141 575 3746 1572 4364
Fragments arranged by size:
4364 3746 1572 1141 575
ApaI G_GGCC'C
Cuts at: 2357 2659 2975 3995 4407 6191 6254 6392 7638
Size: 302 316 1020 412 1784 63 138 1246
Cuts at: 7638 2357
Size: 6117
Fragments arranged by size:
6117 1784 1246 1020 412 316 302 138
63
ApaLI G'TGCA_C
Cuts at: 5657 9455 10611 5657
Size: 3798 1156 6444
Fragments arranged by size:
6444 3798 1156
ApoI r'AATT_y
Cuts at: 1493 7038 7467 8279 8591 8707 8933 9085 11194
Size: 5545 429 812 312 116 226 152 2109
Cuts at: 11194 1493
Size: 1697
Fragments arranged by size:
5545 2109 1697 812 429 312 226 152
116
AscI GG'CGCG_CC
Cuts at: 2268 2268
Size: 11398
AseI AT'TA_AT
Cuts at: 160 1032 6924 9952 160
Size: 872 5892 3028 1606
Fragments arranged by size:
5892 3028 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11398
AvaI C'yCGr_G
Cuts at: 1870 1943 2350 2437 2483 2612 2975 3143 3530
Size: 73 407 87 46 129 363 168 387
Cuts at: 3530 3985 4097 4910 5506 6007 6184 6253 8000
Size: 455 112 813 596 501 177 69 1747
Cuts at: 8000 8723 1870
Size: 723 4545
Fragments arranged by size:
4545 1747 813 723 596 501 455 407
387 363 177 168 129 112 87 73
69 46
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3485 3518 3485
Size: 33 11365
BamHI G'GATC_C
Cuts at: 3385 5510 8728 9095 3385
Size: 2125 3218 367 5688
Fragments arranged by size:
5688 3218 2125 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2312 2447 2697 3269 3341
Size: 325 217 627 525 135 250 572 72
Cuts at: 3341 3702 4375 4771 4789 5345 6030 6409 6451
Size: 361 673 396 18 556 685 379 42
Cuts at: 6451 6607 7482 8719 10174 618
Size: 156 875 1237 1455 1842
Fragments arranged by size:
1842 1455 1237 875 685 673 627 572
556 525 396 379 361 325 250 217
156 135 72 42 18
BanII G_rGCy'C
Cuts at: 1391 2357 2659 2912 2939 2975 3338 3884 3995
Size: 966 302 253 27 36 363 546 111
Cuts at: 3995 4407 5369 5436 5781 5832 6191 6254 6392
Size: 412 962 67 345 51 359 63 138
Cuts at: 6392 7638 7719 8717 1391
Size: 1246 81 998 4072
Fragments arranged by size:
4072 1246 998 966 962 546 412 363
359 345 302 253 138 111 81 67
63 51 36 27
BbeI G_GCGC'C
Cuts at: 2316 2451 3273 4775 4793 6413 6611 7486 2316
Size: 135 822 1502 18 1620 198 875 6228
Fragments arranged by size:
6228 1620 1502 875 822 198 135 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3792 5440 7185 7206 7217 7626 928
Size: 971 1893 1648 1745 21 11 409 4700
Fragments arranged by size:
4700 1893 1745 1648 971 409 21 11
BbvCI CC'TCA_GC
Cuts at: 2274 2999 3447 3558 6330 6526 2274
Size: 725 448 111 2772 196 7146
Fragments arranged by size:
7146 2772 725 448 196 111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 9589 9623 1903
Size: 34 7652 34 3678
Fragments arranged by size:
7652 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3513 4076 5997 6234 7679 8231 9290 10500 3513
Size: 563 1921 237 1445 552 1059 1210 4411
Fragments arranged by size:
4411 1921 1445 1210 1059 563 552 237
BclI T'GATC_A
Cuts at: 1548 2587 7084 1548
Size: 1039 4497 5862
Fragments arranged by size:
5862 4497 1039
BfrBI ATG'CAT
Cuts at: 3962 4264 4438 7785 3962
Size: 302 174 3347 7575
Fragments arranged by size:
7575 3347 302 174
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2565 2654 2742
Size: 107 122 71 631 605 893 89 88
Cuts at: 2742 2864 2979 3020 3293 3302 3489 3615 6114
Size: 122 115 41 273 9 187 126 2499
Cuts at: 6114 6396 10009 136
Size: 282 3613 1525
Fragments arranged by size:
3613 2499 1525 893 631 605 282 273
187 126 122 122 115 107 89 88
71 41 9
BglII A'GATC_T
Cuts at: 3746 3829 11393 3746
Size: 83 7564 3751
Fragments arranged by size:
7564 3751 83
BlpI GC'TnA_GC
Cuts at: 2366 2933 3883 7776 8815 2366
Size: 567 950 3893 1039 4949
Fragments arranged by size:
4949 3893 1039 950 567
Bme1580I G_kGCm'C
Cuts at: 1790 2357 2459 2484 2659 2975 3995 4380 4407
Size: 567 102 25 175 316 1020 385 27
Cuts at: 4407 4476 5661 6033 6191 6254 6392 7638 8126
Size: 69 1185 372 158 63 138 1246 488
Cuts at: 8126 9459 10615 1790
Size: 1333 1156 2573
Fragments arranged by size:
2573 1333 1246 1185 1156 1020 567 488
385 372 316 175 158 138 102 69
63 27 25
BmgBI CAC'GTC
Cuts at: 1318 6496 1318
Size: 5178 6220
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3184 3268 3682
Size: 416 53 83 399 24 1767 84 414
Cuts at: 3682 4374 4539 5118 5530 6967 8318 8455 9129
Size: 692 165 579 412 1437 1351 137 674
Cuts at: 9129 10087 442
Size: 958 1753
Fragments arranged by size:
1767 1753 1437 1351 958 692 674 579
416 414 412 399 165 137 84 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 5013 8561 1056
Size: 3957 3548 3893
Fragments arranged by size:
3957 3893 3548
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5218 5220 5218
Size: 2 11396
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3471 3961 4336 5468 6081 6301 6586 6659
Size: 2232 490 375 1132 613 220 285 73
Cuts at: 6659 7727 8219 10058 1239
Size: 1068 492 1839 2579
Fragments arranged by size:
2579 2232 1839 1132 1068 613 492 490
375 285 220 73
Bpu10I CC'TnA_GC
Cuts at: 2274 2999 3447 3558 6330 6526 2274
Size: 725 448 111 2772 196 7146
Fragments arranged by size:
7146 2772 725 448 196 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4153 4282 5197 5335 8029 9523 10388 10686 4153
Size: 129 915 138 2694 1494 865 298 4865
Fragments arranged by size:
4865 2694 1494 915 865 298 138 129
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3397 7056 8195 10061 882
Size: 617 1898 3659 1139 1866 2219
Fragments arranged by size:
3659 2219 1898 1866 1139 617
BsaAI yAC'GTr
Cuts at: 493 1204 6906 9189 493
Size: 711 5702 2283 2702
Fragments arranged by size:
5702 2702 2283 711
BsaBI GATnn'nnATC
Cuts at: 11392 11392
Size: 11398
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2313 2448 3270 4772
Size: 53 83 186 936 780 135 822 1502
Cuts at: 4772 4790 6410 6575 6608 7257 7483 7587 9205
Size: 18 1620 165 33 649 226 104 1618
Cuts at: 9205 9587 10222 275
Size: 382 635 1451
Fragments arranged by size:
1620 1618 1502 1451 936 822 780 649
635 382 226 186 165 135 104 83
53 33 18
BsaWI w'CCGG_w
Cuts at: 5894 7689 9831 10229 10503 10650 11061 5894
Size: 1795 2142 398 274 147 411 6231
Fragments arranged by size:
6231 2142 1795 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2621 2651 6760 6790 1178
Size: 30 165 30 1218 30 4109 30 5786
Fragments arranged by size:
5786 4109 1218 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2790 2793 2796 2808 2811 2814 2817 2820
Size: 1604 3 3 12 3 3 3 3
Cuts at: 2820 2823 2826 2841 3747 5008 5086 5444 7157
Size: 3 3 15 906 1261 78 358 1713
Cuts at: 7157 7199 7208 7464 7728 7958 8247 1186
Size: 42 9 256 264 230 289 4337
Fragments arranged by size:
4337 1713 1604 1261 906 358 289 264
256 230 78 42 15 12 9 3
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2573 3107 3158 3693 4360 4496 5000 5137 5593
Size: 534 51 535 667 136 504 137 456
Cuts at: 5593 6611 6616 7067 10601 11097 2573
Size: 1018 5 451 3534 496 2874
Fragments arranged by size:
3534 2874 1018 667 535 534 504 496
456 451 137 136 51 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5379 7385 7397 8093 8423 8557 1163
Size: 573 3643 2006 12 696 330 134 4004
Fragments arranged by size:
4004 3643 2006 696 573 330 134 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2425 2475 2508 2569 2859 3012
Size: 275 186 424 50 33 61 290 153
Cuts at: 3012 3240 3306 5173 5959 8848 9609 10637 1540
Size: 228 66 1867 786 2889 761 1028 2301
Fragments arranged by size:
2889 2301 1867 1028 786 761 424 290
275 228 186 153 66 61 50 33
BsiHKAI G_wGCw'C
Cuts at: 2436 2939 3884 5369 5661 7437 7924 9459 9544
Size: 503 945 1485 292 1776 487 1535 85
Cuts at: 9544 10615 2436
Size: 1071 3219
Fragments arranged by size:
3219 1776 1535 1485 1071 945 503 487
292 85
BsmI GAATG_Cn'
Cuts at: 3552 4491 8909 9002 3552
Size: 939 4418 93 5948
Fragments arranged by size:
5948 4418 939 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2003 7722 2003
Size: 5719 5679
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2357 2436 2459 2484 2659 2912 2939
Size: 399 567 79 23 25 175 253 27
Cuts at: 2939 2975 3338 3884 3995 4380 4407 4476 5369
Size: 36 363 546 111 385 27 69 893
Cuts at: 5369 5436 5661 5781 5832 6033 6191 6254 6392
Size: 67 225 120 51 201 158 63 138
Cuts at: 6392 7437 7638 7719 7924 8126 8717 9459 9544
Size: 1045 201 81 205 202 591 742 85
Cuts at: 9544 10615 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1045 893 742 591 567 546
399 385 363 253 225 205 202 201
201 175 158 138 120 111 85 81
79 69 67 63 51 36 27 27
25 23
BspEI T'CCGG_A
Cuts at: 5894 11061 5894
Size: 5167 6231
BspHI T'CATG_A
Cuts at: 6472 9287 11017 6472
Size: 2815 1730 6853
Fragments arranged by size:
6853 2815 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4983 7499 8753 844
Size: 4139 2516 1254 3489
Fragments arranged by size:
4139 3489 2516 1254
BsrBI CCG'CTC
Cuts at: 1394 1823 2553 2837 8586 9285 1394
Size: 429 730 284 5749 699 3507
Fragments arranged by size:
5749 3507 730 699 429 284
BsrDI GCAATG_nn'
Cuts at: 65 1248 5907 6361 7610 7837 9893 10067 65
Size: 1183 4659 454 1249 227 2056 174 1396
Fragments arranged by size:
4659 2056 1396 1249 1183 454 227 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2413 2470 2736 3014 3047 8610 10042
Size: 110 603 57 266 278 33 5563 1432
Cuts at: 10042 10229 1700
Size: 187 2869
Fragments arranged by size:
5563 2869 1432 603 278 266 187 110
57 33
BsrGI T'GTAC_A
Cuts at: 96 5877 6123 6159 96
Size: 5781 246 36 5335
Fragments arranged by size:
5781 5335 246 36
BssHII G'CGCG_C
Cuts at: 1925 2268 3162 1925
Size: 343 894 10161
Fragments arranged by size:
10161 894 343
BssSI C'ACGA_G
Cuts at: 3907 6110 7898 8603 9458 10470 3907
Size: 2203 1788 705 855 1012 4835
Fragments arranged by size:
4835 2203 1788 1012 855 705
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3568 3679 3893 794
Size: 2774 111 214 8299
Fragments arranged by size:
8299 2774 214 111
BstBI TT'CG_AA
Cuts at: 10202 10202
Size: 11398
BstEII G'GTnAC_C
Cuts at: 1184 5573 6724 1184
Size: 4389 1151 5858
Fragments arranged by size:
5858 4389 1151
BstXI CCAn_nnnn'nTGG
Cuts at: 4439 4463 5543 6980 4439
Size: 24 1080 1437 8857
Fragments arranged by size:
8857 1437 1080 24
BstYI r'GATC_y
Cuts at: 1972 3365 3385 3746 3829 5510 6261 8143 8728
Size: 1393 20 361 83 1681 751 1882 585
Cuts at: 8728 9095 9480 9497 10949 11035 11047 11393 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1882 1681 1452 1393 751 585 385
367 361 346 86 83 20 17 12
Bsu36I CC'TnA_GG
Cuts at: 6852 6852
Size: 11398
BtgI C'CryG_G
Cuts at: 513 1065 2004 2859 2916 3240 3357 3537 3858
Size: 552 939 855 57 324 117 180 321
Cuts at: 3858 4797 5226 5556 6705 8343 8562 513
Size: 939 429 330 1149 1638 219 3349
Fragments arranged by size:
3349 1638 1149 939 939 855 552 429
330 324 321 219 180 117 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3344 7222 7236 7735 7795 487
Size: 683 56 2118 3878 14 499 60 4090
Fragments arranged by size:
4090 3878 2118 683 499 60 56 14
BtsI GCAGTG_nn'
Cuts at: 4641 5362 6584 8914 9708 9728 4641
Size: 721 1222 2330 794 20 6311
Fragments arranged by size:
6311 2330 1222 794 721 20
ClaI AT'CG_AT
Cuts at: 1554 7581 1554
Size: 6027 5371
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11363
DraI TTT'AAA
Cuts at: 8702 8702
Size: 11398
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5536 6580 10405 809
Size: 507 4220 1044 3825 1802
Fragments arranged by size:
4220 3825 1802 1044 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2335 2422 2505
Size: 54 1425 50 275 18 505 87 83
Cuts at: 2505 2566 2856 3009 3049 3226 3237 3303 3540
Size: 61 290 153 40 177 11 66 237
Cuts at: 3540 5063 5611 7601 8034 8250 8544 8565 8845
Size: 1523 548 1990 433 216 294 21 280
Cuts at: 8845 9734 11363 8
Size: 889 1629 43
Fragments arranged by size:
1990 1629 1523 1425 889 548 505 433
294 290 280 275 237 216 177 153
87 83 66 61 54 50 43 40
21 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 2422 2505 2566 2856 3009 3237 3303
Size: 275 610 83 61 290 153 228 66
Cuts at: 3303 8845 1537
Size: 5542 4090
Fragments arranged by size:
5542 4090 610 290 275 228 153 83
66 61
EarI CTCTTCn'nnn_
Cuts at: 1353 3395 5187 7542 9328 1353
Size: 2042 1792 2355 1786 3423
Fragments arranged by size:
3423 2355 2042 1792 1786
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2613 2623 2629 2787 9985 10359
Size: 1064 138 1024 10 6 158 7198 374
Cuts at: 10359 10505 387
Size: 146 1280
Fragments arranged by size:
7198 1280 1064 1024 374 158 146 138
10 6
EcoICRI GAG'CTC
Cuts at: 2937 3882 5367 2937
Size: 945 1485 8968
Fragments arranged by size:
8968 1485 945
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3471 3961 4336 5434 5468 5600 6081
Size: 393 1839 490 375 1098 34 132 481
Cuts at: 6081 6301 6586 6659 7566 7638 7727 8219 9461
Size: 220 285 73 907 72 89 492 1242
Cuts at: 9461 10058 10845 11113 1239
Size: 597 787 268 1524
Fragments arranged by size:
1839 1524 1242 1098 907 787 597 492
490 481 393 375 285 268 220 132
89 73 72 34
EcoNI CCTnn'n_nnAGG
Cuts at: 3002 6232 6420 3002
Size: 3230 188 7980
Fragments arranged by size:
7980 3230 188
EcoO109I rG'GnC_Cy
Cuts at: 2655 2656 2972 3991 4403 5788 5999 6250 6326
Size: 1 316 1019 412 1385 211 251 76
Cuts at: 6326 6388 6389 7635 8162 2655
Size: 62 1 1246 527 5891
Fragments arranged by size:
5891 1385 1246 1019 527 412 316 251
211 76 62 1 1
EcoRI G'AATT_C
Cuts at: 7038 8591 8707 9085 7038
Size: 1553 116 378 9351
Fragments arranged by size:
9351 1553 378 116
EcoRV GAT'ATC
Cuts at: 6841 8623 8631 6841
Size: 1782 8 9608
Fragments arranged by size:
9608 1782 8
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4323 5809 7678 4323
Size: 1486 1869 8043
Fragments arranged by size:
8043 1869 1486
FspI TGC'GCA
Cuts at: 9904 9904
Size: 11398
HaeII r_GCGC'y
Cuts at: 1678 2316 2451 2541 3043 3273 4775 4793 5929
Size: 638 135 90 502 230 1502 18 1136
Cuts at: 5929 6109 6413 6611 6634 7486 8351 10545 1678
Size: 180 304 198 23 852 865 2194 2531
Fragments arranged by size:
2531 2194 1502 1136 865 852 638 502
304 230 198 180 135 90 23 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3073 3105 3559 3591 4297
Size: 32 135 32 1501 32 454 32 706
Cuts at: 4297 4329 4826 4858 4904 4936 5247 5279 5654
Size: 32 497 32 46 32 311 32 375
Cuts at: 5654 5686 6454 6486 7100 7132 7577 7609 10039
Size: 32 768 32 614 32 445 32 2430
Cuts at: 10039 10071 10113 10145 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2430 1501 768 706 614 497 454
445 375 311 135 46 42 32 32
32 32 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5170 5541 7351 8742 8988 669
Size: 988 281 3232 371 1810 1391 246 3079
Fragments arranged by size:
3232 3079 1810 1391 988 371 281 246
HindIII A'AGCT_T
Cuts at: 748 8431 748
Size: 7683 3715
HpaI GTT'AAC
Cuts at: 8988 8988
Size: 11398
KasI G'GCGC_C
Cuts at: 2312 2447 3269 4771 4789 6409 6607 7482 2312
Size: 135 822 1502 18 1620 198 875 6228
Fragments arranged by size:
6228 1620 1502 875 822 198 135 18
KpnI G_GTAC'C
Cuts at: 2701 3706 6455 8723 2701
Size: 1005 2749 2268 5376
Fragments arranged by size:
5376 2749 2268 1005
MfeI C'AATT_G
Cuts at: 3507 8997 3507
Size: 5490 5908
MluI A'CGCG_T
Cuts at: 11274 11274
Size: 11398
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2259 3503 5278 8177 10512 10696 1305
Size: 954 1244 1775 2899 2335 184 2007
Fragments arranged by size:
2899 2335 2007 1775 1244 954 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5065 8036 8252 8567 10
Size: 54 1425 343 3233 2971 216 315 2841
Fragments arranged by size:
3233 2971 2841 1425 343 315 216 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2094 2115 2966 5031 5126 5186 5744
Size: 648 928 21 851 2065 95 60 558
Cuts at: 5744 7790 8399 8492 9356 9715 9874 518
Size: 2046 609 93 864 359 159 2042
Fragments arranged by size:
2065 2046 2042 928 864 851 648 609
558 359 159 95 93 60 21
MspA1I CmG'CkG
Cuts at: 1606 2362 2790 2861 2918 3094 3242 3284 3499
Size: 756 428 71 57 176 148 42 215
Cuts at: 3499 4395 4496 6304 7262 8636 8814 9491 10639
Size: 896 101 1808 958 1374 178 677 1148
Cuts at: 10639 10884 1606
Size: 245 2120
Fragments arranged by size:
2120 1808 1374 1148 958 896 756 677
428 245 215 178 176 148 101 71
57 42
NaeI GCC'GGC
Cuts at: 2415 2738 3016 3049 2415
Size: 323 278 33 10764
Fragments arranged by size:
10764 323 278 33
NarI GG'CG_CC
Cuts at: 2313 2448 3270 4772 4790 6410 6608 7483 2313
Size: 135 822 1502 18 1620 198 875 6228
Fragments arranged by size:
6228 1620 1502 875 822 198 135 18
NcoI C'CATG_G
Cuts at: 513 1065 2004 3357 3537 4797 5556 8343 8562
Size: 552 939 1353 180 1260 759 2787 219
Cuts at: 8562 513
Size: 3349
Fragments arranged by size:
3349 2787 1353 1260 939 759 552 219
180
NdeI CA'TA_TG
Cuts at: 387 6075 387
Size: 5688 5710
NgoMIV G'CCGG_C
Cuts at: 2413 2736 3014 3047 2413
Size: 323 278 33 10764
Fragments arranged by size:
10764 323 278 33
NheI G'CTAG_C
Cuts at: 1052 5009 8557 1052
Size: 3957 3548 3893
Fragments arranged by size:
3957 3893 3548
NotI GC'GGCC_GC
Cuts at: 2422 2566 2856 3009 3237 3303 8845 2422
Size: 144 290 153 228 66 5542 4975
Fragments arranged by size:
5542 4975 290 228 153 144 66
NsiI A_TGCA'T
Cuts at: 3964 4266 4440 7787 3964
Size: 302 174 3347 7575
Fragments arranged by size:
7575 3347 302 174
NspI r_CATG'y
Cuts at: 1132 1229 3724 3962 4268 4561 5749 5869 5878
Size: 97 2495 238 306 293 1188 120 9
Cuts at: 5878 6088 6124 6133 6652 8497 8756 10301 1132
Size: 210 36 9 519 1845 259 1545 2229
Fragments arranged by size:
2495 2229 1845 1545 1188 519 306 293
259 238 210 120 97 36 9 9
PasI CC'CwG_GG
Cuts at: 1348 2452 3370 4400 4477 7631 1348
Size: 1104 918 1030 77 3154 5115
Fragments arranged by size:
5115 3154 1104 1030 918 77
PciI A'CATG_T
Cuts at: 3720 5874 6120 10297 3720
Size: 2154 246 4177 4821
Fragments arranged by size:
4821 4177 2154 246
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4496 4520 7120 1108
Size: 3388 24 2600 5386
Fragments arranged by size:
5386 3388 2600 24
PfoI T'CCnGG_A
Cuts at: 2511 4519 8223 2511
Size: 2008 3704 5686
Fragments arranged by size:
5686 3704 2008
PmeI GTTT'AAAC
Cuts at: 8702 8702
Size: 11398
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3733 3765 4733 4765 9467 9499 10980 11012 3733
Size: 32 968 32 4702 32 1481 32 4119
Fragments arranged by size:
4702 4119 1481 968 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5788 8162 5788
Size: 2374 9024
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 11398
PsiI TTA'TAA
Cuts at: 8968 8968
Size: 11398
PspOMI G'GGCC_C
Cuts at: 2353 2655 2971 3991 4403 6187 6250 6388 7634
Size: 302 316 1020 412 1784 63 138 1246
Cuts at: 7634 2353
Size: 6117
Fragments arranged by size:
6117 1784 1246 1020 412 316 302 138
63
PspXI vC'TCGA_Gb
Cuts at: 8000 8000
Size: 11398
PstI C_TGCA'G
Cuts at: 830 2682 6309 7267 7973 8134 8750 830
Size: 1852 3627 958 706 161 616 3478
Fragments arranged by size:
3627 3478 1852 958 706 616 161
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11398
PvuII CAG'CTG
Cuts at: 3094 4496 8636 3094
Size: 1402 4140 5856
Fragments arranged by size:
5856 4140 1402
RsrII CG'GwC_CG
Cuts at: 3350 6193 3350
Size: 2843 8555
SacI G_AGCT'C
Cuts at: 2939 3884 5369 2939
Size: 945 1485 8968
Fragments arranged by size:
8968 1485 945
SacII CC_GC'GG
Cuts at: 2862 2919 3243 2862
Size: 57 324 11017
Fragments arranged by size:
11017 324 57
SalI G'TCGA_C
Cuts at: 1936 5168 8740 1936
Size: 3232 3572 4594
Fragments arranged by size:
4594 3572 3232
SanDI GG'GwC_CC
Cuts at: 8162 8162
Size: 11398
SapI GCTCTTCn'nnn_
Cuts at: 7542 7542
Size: 11398
SbfI CC_TGCA'GG
Cuts at: 8750 8750
Size: 11398
ScaI AGT'ACT
Cuts at: 7007 9646 11358 7007
Size: 2639 1712 7047
Fragments arranged by size:
7047 2639 1712
SfcI C'TryA_G
Cuts at: 826 1045 2147 2678 2731 4020 4390 4726 4780
Size: 219 1102 531 53 1289 370 336 54
Cuts at: 4780 4933 6229 6305 7263 7969 8130 8746 9881
Size: 153 1296 76 958 706 161 616 1135
Cuts at: 9881 10562 10753 826
Size: 681 191 1471
Fragments arranged by size:
1471 1296 1289 1135 1102 958 706 681
616 531 370 336 219 191 161 153
76 54 53
SfiI GGCCn_nnn'nGGCC
Cuts at: 2565 3302 3489 6396 2565
Size: 737 187 2907 7567
Fragments arranged by size:
7567 2907 737 187
SfoI GGC'GCC
Cuts at: 2314 2449 3271 4773 4791 6411 6609 7484 2314
Size: 135 822 1502 18 1620 198 875 6228
Fragments arranged by size:
6228 1620 1502 875 822 198 135 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11398
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11398
SmaI CCC'GGG
Cuts at: 1872 2352 2439 2485 2614 2977 3532 3987 4099
Size: 480 87 46 129 363 555 455 112
Cuts at: 4099 6255 8725 1872
Size: 2156 2470 4545
Fragments arranged by size:
4545 2470 2156 555 480 455 363 129
112 87 46
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4132 4297 5212 5350 7498 8000
Size: 197 926 2189 165 915 138 2148 502
Cuts at: 8000 8008 9502 10403 10665 820
Size: 8 1494 901 262 1553
Fragments arranged by size:
2189 2148 1553 1494 926 915 901 502
262 197 165 138 8
SnaBI TAC'GTA
Cuts at: 493 9189 493
Size: 8696 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11398
SphI G_CATG'C
Cuts at: 1132 4268 5749 5869 8756 1132
Size: 3136 1481 120 2887 3774
Fragments arranged by size:
3774 3136 2887 1481 120
SspI AAT'ATT
Cuts at: 5 52 6928 7801 9322 5
Size: 47 6876 873 1521 2081
Fragments arranged by size:
6876 2081 1521 873 47
StuI AGG'CCT
Cuts at: 1517 7860 1517
Size: 6343 5055
StyI C'CwwG_G
Cuts at: 513 1065 2004 2316 3357 3537 3916 4797 5556
Size: 552 939 312 1041 180 379 881 759
Cuts at: 5556 5995 7119 8343 8368 8547 8562 8837 513
Size: 439 1124 1224 25 179 15 275 3074
Fragments arranged by size:
3074 1224 1124 1041 939 881 759 552
439 379 312 275 180 179 25 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3367 5187 5973 7368 7658 8161 9425
Size: 1255 1787 1820 786 1395 290 503 1264
Cuts at: 9425 9442 9595 9780 10225 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1820 1787 1498 1395 1264 1255 786 503
445 290 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3147 5645 5877
Size: 274 80 33 51 1443 1170 2498 232
Cuts at: 5877 6123 6135 6159 7005 8674 9644 11356 96
Size: 246 12 24 846 1669 970 1712 138
Fragments arranged by size:
2498 1712 1669 1443 1170 970 846 274
246 232 138 80 51 33 24 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2621 2653 6261 6293 6760 6792 11358 11390 2621
Size: 32 3608 32 467 32 4566 32 2629
Fragments arranged by size:
4566 3608 2629 467 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11398
XbaI T'CTAG_A
Cuts at: 8734 8734
Size: 11398
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4040 4265 8569 4040
Size: 225 4304 6869
Fragments arranged by size:
6869 4304 225
XhoI C'TCGA_G
Cuts at: 1943 8000 1943
Size: 6057 5341
XmaI C'CCGG_G
Cuts at: 1870 2350 2437 2483 2612 2975 3530 3985 4097
Size: 480 87 46 129 363 555 455 112
Cuts at: 4097 6253 8723 1870
Size: 2156 2470 4545
Fragments arranged by size:
4545 2470 2156 555 480 455 363 129
112 87 46
XmnI GAAnn'nnTTC
Cuts at: 9527 9527
Size: 11398
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7588 9206 276
Size: 53 83 186 936 6054 1618 2468
Fragments arranged by size:
6054 2468 1618 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AvrII BsiWI BstZ17I DraIII FseI FspAI
NruI PacI PmlI PsrI SexAI SrfI SwaI