Restriction map for
pFN21AE1597
(Circular) (Six-base) MAPSORT of: pFN21AE1597.seq Check: 7930 from: 1 to: 13945
pFN21AE1597 13945 bp
With 182 enzymes: SgfI *
September 9, 2010 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3379 3565 6733 3379
Size: 186 3168 10591
Fragments arranged by size:
10591 3168 186
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2640 11755 278
Size: 53 83 186 936 1104 9115 2468
Fragments arranged by size:
9115 2468 1104 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3242 3638 4304 5501 6074 10202 11288 1937
Size: 1305 396 666 1197 573 4128 1086 4594
Fragments arranged by size:
4594 4128 1305 1197 1086 666 573 396
Acc65I G'GTAC_C
Cuts at: 4142 5194 11266 4142
Size: 1052 6072 6821
Fragments arranged by size:
6821 6072 1052
AclI AA'CG_TT
Cuts at: 1563 9561 12072 12445 1563
Size: 7998 2511 373 3063
Fragments arranged by size:
7998 3063 2511 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3030 3153 3756 4815 5253 5664 5934 6336
Size: 1398 123 603 1059 438 411 270 402
Cuts at: 6336 6768 7554 7689 7977 8365 11172 12008 13392
Size: 432 786 135 288 388 2807 836 1384
Cuts at: 13392 13660 1632
Size: 268 1917
Fragments arranged by size:
2807 1917 1398 1384 1059 836 786 603
438 432 411 402 388 288 270 268
135 123
AfeI AGC'GCT
Cuts at: 10236 10236
Size: 13945
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 13748
AflIII A'CryG_T
Cuts at: 1203 3743 9127 9723 10503 12844 13821 1203
Size: 2540 5384 596 780 2341 977 1327
Fragments arranged by size:
5384 2540 2341 1327 977 780 596
AgeI A'CCGG_T
Cuts at: 12776 12776
Size: 13945
AhdI GACnn_n'nnGTC
Cuts at: 12674 12674
Size: 13945
AleI CACnn'nnGTG
Cuts at: 1166 4031 5360 5483 5729 6209 6839 8600 1166
Size: 2865 1329 123 246 480 630 1761 6511
Fragments arranged by size:
6511 2865 1761 1329 630 480 246 123
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 13945
AlwNI CAG_nnn'CTG
Cuts at: 3788 3994 4580 4646 8000 8624 9776 11688 13260
Size: 206 586 66 3354 624 1152 1912 1572
Cuts at: 13260 3788
Size: 4473
Fragments arranged by size:
4473 3354 1912 1572 1152 624 586 206
66
ApaLI G'TGCA_C
Cuts at: 10354 12002 13158 10354
Size: 1648 1156 11141
Fragments arranged by size:
11141 1648 1156
ApoI r'AATT_y
Cuts at: 1493 3148 3595 4045 4262 4396 4848 5848 5922
Size: 1655 447 450 217 134 452 1000 74
Cuts at: 5922 5983 7589 7836 8478 8810 8821 8830 8852
Size: 61 1606 247 642 332 11 9 22
Cuts at: 8852 9433 9618 9852 10102 10338 10559 10765 11254
Size: 581 185 234 250 236 221 206 489
Cuts at: 11254 11480 11632 13741 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1655 1606 1000 642 581 489
452 450 447 332 250 247 236 234
226 221 217 206 185 152 134 74
61 22 11 9
AseI AT'TA_AT
Cuts at: 160 1032 7701 12499 160
Size: 872 6669 4798 1606
Fragments arranged by size:
6669 4798 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13945
AvaI C'yCGr_G
Cuts at: 1870 1943 5868 11270 1870
Size: 73 3925 5402 4545
Fragments arranged by size:
5402 4545 3925 73
AvrII C'CTAG_G
Cuts at: 2482 4763 9787 2482
Size: 2281 5024 6640
Fragments arranged by size:
6640 5024 2281
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4170 4203 4464 4497 7191 7224 4170
Size: 33 261 33 2694 33 10891
Fragments arranged by size:
10891 2694 261 33 33 33
BamHI G'GATC_C
Cuts at: 8784 11275 11642 8784
Size: 2491 367 11087
Fragments arranged by size:
11087 2491 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3901 4142 5194 5612 6910
Size: 325 217 627 2114 241 1052 418 1298
Cuts at: 6910 7022 7985 8432 11266 12721 618
Size: 112 963 447 2834 1455 1842
Fragments arranged by size:
2834 2114 1842 1455 1298 1052 963 627
447 418 325 241 217 112
BanII G_rGCy'C
Cuts at: 1391 10646 11264 1391
Size: 9255 618 4072
Fragments arranged by size:
9255 4072 618
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2268 2640 3206 5303 6774 8622 10519
Size: 971 369 372 566 2097 1471 1848 1897
Cuts at: 10519 10796 928
Size: 277 4077
Fragments arranged by size:
4077 2097 1897 1848 1471 971 566 372
369 277
BbvCI CC'TCA_GC
Cuts at: 4581 5017 5082 9408 4581
Size: 436 65 4326 9118
Fragments arranged by size:
9118 4326 436 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8688 8722 12136 12170 1903
Size: 34 6751 34 3414 34 3678
Fragments arranged by size:
6751 3678 3414 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2421 4198 6232 11837 13047 2421
Size: 1777 2034 5605 1210 3319
Fragments arranged by size:
5605 3319 2034 1777 1210
BclI T'GATC_A
Cuts at: 1548 10344 10389 1548
Size: 8796 45 5104
Fragments arranged by size:
8796 5104 45
BfrBI ATG'CAT
Cuts at: 2178 6634 9246 2178
Size: 4456 2612 6877
Fragments arranged by size:
6877 4456 2612
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3998 5603 9695
Size: 107 122 71 631 605 2326 1605 4092
Cuts at: 9695 12556 136
Size: 2861 1525
Fragments arranged by size:
4092 2861 2326 1605 1525 631 605 122
107 71
BglII A'GATC_T
Cuts at: 7542 10624 13940 7542
Size: 3082 3316 7547
Fragments arranged by size:
7547 3316 3082
BlpI GC'TnA_GC
Cuts at: 11362 11362
Size: 13945
Bme1580I G_kGCm'C
Cuts at: 1790 2490 3286 6160 10358 12006 13162 1790
Size: 700 796 2874 4198 1648 1156 2573
Fragments arranged by size:
4198 2874 2573 1648 1156 796 700
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 13945
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3288 3711 8243
Size: 416 53 83 399 24 1871 423 4532
Cuts at: 8243 8607 9995 11676 12634 442
Size: 364 1388 1681 958 1753
Fragments arranged by size:
4532 1871 1753 1681 1388 958 423 416
399 364 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 13945
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3679 6709 8344 9873 3679
Size: 3030 1635 1529 7751
Fragments arranged by size:
7751 3030 1635 1529
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2793 3423 3937 4113 7080 8314 8592 10511
Size: 1554 630 514 176 2967 1234 278 1919
Cuts at: 10511 12605 1239
Size: 2094 2579
Fragments arranged by size:
2967 2579 2094 1919 1554 1234 630 514
278 176
Bpu10I CC'TnA_GC
Cuts at: 3411 4581 5017 5082 5370 8517 9022 9408 3411
Size: 1170 436 65 288 3147 505 386 7948
Fragments arranged by size:
7948 3147 1170 505 436 386 288 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2191 2480 5514 6022 11114 12070 12935 13233 2191
Size: 289 3034 508 5092 956 865 298 2903
Fragments arranged by size:
5092 3034 2903 956 865 508 298 289
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2280 3542 3650 3956 4418 5372 5615
Size: 617 781 1262 108 306 462 954 243
Cuts at: 5615 5633 5906 12608 882
Size: 18 273 6702 2219
Fragments arranged by size:
6702 2219 1262 954 781 617 462 306
273 243 108 18
BsaAI yAC'GTr
Cuts at: 493 1204 3746 11736 493
Size: 711 2542 7990 2702
Fragments arranged by size:
7990 2702 2542 711
BsaBI GATnn'nnATC
Cuts at: 13939 13939
Size: 13945
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2637 11120 11752 12134
Size: 53 83 186 936 1104 8483 632 382
Cuts at: 12134 12769 275
Size: 635 1451
Fragments arranged by size:
8483 1451 1104 936 635 632 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 10250 12378 12776 13050 13197 13608 10250
Size: 2128 398 274 147 411 10587
Fragments arranged by size:
10587 2128 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2860 2890 3022 3052 3421
Size: 30 165 30 1457 30 132 30 369
Cuts at: 3421 3451 6511 6541 7549 7579 10272 10302 1178
Size: 30 3060 30 1008 30 2693 30 4821
Fragments arranged by size:
4821 3060 2693 1457 1008 369 165 132
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3652 5099 5552 7554 7557 10255 1186
Size: 2466 1447 453 2002 3 2698 4876
Fragments arranged by size:
4876 2698 2466 2002 1447 453 3
BseYI C'CCAG_C
Cuts at: 6851 6998 9451 10090 13148 13644 6851
Size: 147 2453 639 3058 496 7152
Fragments arranged by size:
7152 3058 2453 639 496 147
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2778 2883 10729 11157 1163
Size: 573 1042 105 7846 428 3951
Fragments arranged by size:
7846 3951 1042 573 428 105
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 11395 12156 13184 1540
Size: 275 186 9394 761 1028 2301
Fragments arranged by size:
9394 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3423 4880 5148 7058 8201 10358 11109 12006 12091
Size: 1457 268 1910 1143 2157 751 897 85
Cuts at: 12091 13162 3423
Size: 1071 4206
Fragments arranged by size:
4206 2157 1910 1457 1143 1071 897 751
268 85
BsmI GAATG_Cn'
Cuts at: 4386 6322 9409 9747 11456 11549 4386
Size: 1936 3087 338 1709 93 6782
Fragments arranged by size:
6782 3087 1936 1709 338 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2490 3286 3423 4880 5148 6160 7058
Size: 399 700 796 137 1457 268 1012 898
Cuts at: 7058 8201 10358 10646 11109 11264 12006 12091 13162
Size: 1143 2157 288 463 155 742 85 1071
Cuts at: 13162 1391
Size: 2174
Fragments arranged by size:
2174 2157 1457 1143 1071 1012 898 796
742 700 463 399 288 268 155 137
85
BspEI T'CCGG_A
Cuts at: 13608 13608
Size: 13945
BspHI T'CATG_A
Cuts at: 4063 10725 11834 13564 4063
Size: 6662 1109 1730 4444
Fragments arranged by size:
6662 4444 1730 1109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3379 3565 4321 6733 11300 844
Size: 2535 186 756 2412 4567 3489
Fragments arranged by size:
4567 3489 2535 2412 756 186
BsrBI CCG'CTC
Cuts at: 1394 1823 11832 1394
Size: 429 10009 3507
Fragments arranged by size:
10009 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3964 5051 6548 7818 9619 9799 12440
Size: 1183 2716 1087 1497 1270 1801 180 2641
Cuts at: 12440 12614 65
Size: 174 1396
Fragments arranged by size:
2716 2641 1801 1497 1396 1270 1183 1087
180 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 11052 12589 12776 1700
Size: 110 9242 1537 187 2869
Fragments arranged by size:
9242 2869 1537 187 110
BsrGI T'GTAC_A
Cuts at: 96 3715 5719 6659 8301 96
Size: 3619 2004 940 1642 5740
Fragments arranged by size:
5740 3619 2004 1642 940
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 13945
BssSI C'ACGA_G
Cuts at: 6151 12005 13017 6151
Size: 5854 1012 7079
Fragments arranged by size:
7079 5854 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 10877 794
Size: 10083 3862
BstBI TT'CG_AA
Cuts at: 10318 12749 10318
Size: 2431 11514
BstEII G'GTnAC_C
Cuts at: 1184 5095 1184
Size: 3911 10034
BstXI CCAn_nnnn'nTGG
Cuts at: 2771 5191 5743 5797 6184 6994 7723 10043 10276
Size: 2420 552 54 387 810 729 2320 233
Cuts at: 10276 2771
Size: 6440
Fragments arranged by size:
6440 2420 2320 810 729 552 387 233
54
BstYI r'GATC_y
Cuts at: 1972 4859 7542 7997 8784 9373 10624 11224 11275
Size: 2887 2683 455 787 589 1251 600 51
Cuts at: 11275 11642 12027 12044 13496 13582 13594 13940 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2887 2683 1977 1452 1251 787 600 589
455 385 367 346 86 51 17 12
Bsu36I CC'TnA_GG
Cuts at: 6342 8319 6342
Size: 1977 11968
BtgI C'CryG_G
Cuts at: 513 1065 3081 4205 5245 5277 5304 7469 7849
Size: 552 2016 1124 1040 32 27 2165 380
Cuts at: 7849 513
Size: 6609
Fragments arranged by size:
6609 2165 2016 1124 1040 552 380 32
27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 13206
Fragments arranged by size:
13206 683 56
BtsI GCAGTG_nn'
Cuts at: 2678 2952 4146 4581 7026 9141 10293 10449 10997
Size: 274 1194 435 2445 2115 1152 156 548
Cuts at: 10997 11461 12255 12275 2678
Size: 464 794 20 4348
Fragments arranged by size:
4348 2445 2115 1194 1152 794 548 464
435 274 156 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 13945
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5209 5244 6739 6774 9931 9966 529
Size: 35 4645 35 1495 35 3157 35 4508
Fragments arranged by size:
4645 4508 3157 1495 35 35 35 35
DraI TTT'AAA
Cuts at: 4267 9432 11249 4267
Size: 5165 1817 6963
Fragments arranged by size:
6963 5165 1817
DraIII CAC_nnn'GTG
Cuts at: 8237 9512 10966 8237
Size: 1275 1454 11216
Fragments arranged by size:
11216 1454 1275
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 5833 12952 809
Size: 507 3650 867 7119 1802
Fragments arranged by size:
7119 3650 1802 867 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3304 5121 6232
Size: 54 1425 50 275 18 1474 1817 1111
Cuts at: 6232 9687 9895 11392 12281 13910 8
Size: 3455 208 1497 889 1629 43
Fragments arranged by size:
3455 1817 1629 1497 1474 1425 1111 889
275 208 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 11392 1537
Size: 275 9580 4090
Fragments arranged by size:
9580 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2110 2414 2583 6742 8769 8901 9022 9462
Size: 757 304 169 4159 2027 132 121 440
Cuts at: 9462 11041 11875 1353
Size: 1579 834 3423
Fragments arranged by size:
4159 3423 2027 1579 834 757 440 304
169 132 121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 9577 10813 12532 12906 13052 387
Size: 1064 138 7988 1236 1719 374 146 1280
Fragments arranged by size:
7988 1719 1280 1236 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2793 3030 3153 3423 3756 3937 4113
Size: 393 1161 237 123 270 333 181 176
Cuts at: 4113 4815 5253 5664 5934 6336 6768 7080 7554
Size: 702 438 411 270 402 432 312 474
Cuts at: 7554 7689 7977 8314 8365 8592 10511 11172 12008
Size: 135 288 337 51 227 1919 661 836
Cuts at: 12008 12605 13392 13660 1239
Size: 597 787 268 1524
Fragments arranged by size:
1919 1524 1161 836 787 702 661 597
474 438 432 411 402 393 337 333
312 288 270 270 268 237 227 181
176 135 123 51
EcoNI CCTnn'n_nnAGG
Cuts at: 3909 7830 9804 3909
Size: 3921 1974 8050
Fragments arranged by size:
8050 3921 1974
EcoO109I rG'GnC_Cy
Cuts at: 3914 3991 7176 9195 10886 3914
Size: 77 3185 2019 1691 6973
Fragments arranged by size:
6973 3185 2019 1691 77
EcoRI G'AATT_C
Cuts at: 3148 5848 7836 8478 8830 9852 10102 10765 11254
Size: 2700 1988 642 352 1022 250 663 489
Cuts at: 11254 11632 3148
Size: 378 5461
Fragments arranged by size:
5461 2700 1988 1022 663 642 489 378
352 250
EcoRV GAT'ATC
Cuts at: 4923 6282 8148 4923
Size: 1359 1866 10720
Fragments arranged by size:
10720 1866 1359
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 7438 11116 7438
Size: 3678 10267
FspI TGC'GCA
Cuts at: 12451 12451
Size: 13945
HaeII r_GCGC'y
Cuts at: 1678 10238 13092 1678
Size: 8560 2854 2531
Fragments arranged by size:
8560 2854 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2031 2063 2189 2221 2942
Size: 32 135 32 459 32 126 32 721
Cuts at: 2942 2974 3218 3250 3620 3652 4355 4387 6271
Size: 32 244 32 370 32 703 32 1884
Cuts at: 6271 6303 6386 6418 6994 7026 7534 7549 7566
Size: 32 83 32 576 32 508 15 17
Cuts at: 7566 7581 8132 8164 8350 8382 9358 9390 9661
Size: 15 551 32 186 32 976 32 271
Cuts at: 9661 9693 10245 10277 10416 10448 11110 11142 12586
Size: 32 552 32 139 32 662 32 1444
Cuts at: 12586 12618 12660 12692 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1884 1444 976 721 703 662 576
552 551 508 459 370 271 244 186
139 135 126 83 42 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 17 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2650 2671 3231 5673 7764 11118
Size: 988 281 712 21 560 2442 2091 3354
Cuts at: 11118 11136 11289 11535 669
Size: 18 153 246 3079
Fragments arranged by size:
3354 3079 2442 2091 988 712 560 281
246 153 21 18
HindIII A'AGCT_T
Cuts at: 748 2030 11197 748
Size: 1282 9167 3496
Fragments arranged by size:
9167 3496 1282
HpaI GTT'AAC
Cuts at: 11535 11535
Size: 13945
KpnI G_GTAC'C
Cuts at: 4146 5198 11270 4146
Size: 1052 6072 6821
Fragments arranged by size:
6821 6072 1052
MfeI C'AATT_G
Cuts at: 4452 7369 9101 11544 4452
Size: 2917 1732 2443 6853
Fragments arranged by size:
6853 2917 2443 1732
MluI A'CGCG_T
Cuts at: 13821 13821
Size: 13945
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5796 6474 6971 8507 10643 11050 13059 13243
Size: 4491 678 497 1536 2136 407 2009 184
Cuts at: 13243 1305
Size: 2007
Fragments arranged by size:
4491 2136 2009 2007 1536 678 497 407
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3306 5123 6234 9897 10
Size: 54 1425 343 1474 1817 1111 3663 4058
Fragments arranged by size:
4058 3663 1817 1474 1425 1111 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2356 4031 4204 4812 5360 5483 5729
Size: 648 1190 1675 173 608 548 123 246
Cuts at: 5729 6209 6839 6992 7811 8467 8600 9756 10694
Size: 480 630 153 819 656 133 1156 938
Cuts at: 10694 10952 11903 12262 12421 518
Size: 258 951 359 159 2042
Fragments arranged by size:
2042 1675 1190 1156 951 938 819 656
648 630 608 548 480 359 258 246
173 159 153 133 123
MspA1I CmG'CkG
Cuts at: 1606 3302 3329 3434 3572 3848 4184 4856 5006
Size: 1696 27 105 138 276 336 672 150
Cuts at: 5006 6416 7667 10439 11361 12038 13186 13431 1606
Size: 1410 1251 2772 922 677 1148 245 2120
Fragments arranged by size:
2772 2120 1696 1410 1251 1148 922 677
672 336 276 245 150 138 105 27
NcoI C'CATG_G
Cuts at: 513 1065 3081 4205 5245 5304 7469 7849 513
Size: 552 2016 1124 1040 59 2165 380 6609
Fragments arranged by size:
6609 2165 2016 1124 1040 552 380 59
NdeI CA'TA_TG
Cuts at: 387 2128 5043 6229 387
Size: 1741 2915 1186 8103
Fragments arranged by size:
8103 2915 1741 1186
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 13945
NotI GC'GGCC_GC
Cuts at: 11392 11392
Size: 13945
NsiI A_TGCA'T
Cuts at: 2180 6636 9248 2180
Size: 4456 2612 6877
Fragments arranged by size:
6877 4456 2612
NspI r_CATG'y
Cuts at: 1132 1229 2178 10507 11303 12848 1132
Size: 97 949 8329 796 1545 2229
Fragments arranged by size:
8329 2229 1545 949 796 97
PasI CC'CwG_GG
Cuts at: 1348 4900 5797 9457 1348
Size: 3552 897 3660 5836
Fragments arranged by size:
5836 3660 3552 897
PciI A'CATG_T
Cuts at: 10503 12844 10503
Size: 2341 11604
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2578 5063 1108
Size: 1470 2485 9990
Fragments arranged by size:
9990 2485 1470
PfoI T'CCnGG_A
Cuts at: 2577 6917 7649 8569 2577
Size: 4340 732 920 7953
Fragments arranged by size:
7953 4340 920 732
PmeI GTTT'AAAC
Cuts at: 11249 11249
Size: 13945
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 12014 12046 13527 13559 12014
Size: 32 1481 32 12400
Fragments arranged by size:
12400 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3914 7176 9195 10886 3914
Size: 3262 2019 1691 6973
Fragments arranged by size:
6973 3262 2019 1691
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 13945
PsiI TTA'TAA
Cuts at: 8804 10241 11515 8804
Size: 1437 1274 11234
Fragments arranged by size:
11234 1437 1274
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6963 6995 8206 8238 6963
Size: 32 1211 32 12670
Fragments arranged by size:
12670 1211 32 32
PstI C_TGCA'G
Cuts at: 830 10020 10444 11297 830
Size: 9190 424 853 3478
Fragments arranged by size:
9190 3478 853 424
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 13945
PvuII CAG'CTG
Cuts at: 3302 3329 3434 3572 3848 4184 4856 5006 6416
Size: 27 105 138 276 336 672 150 1410
Cuts at: 6416 7667 10439 3302
Size: 1251 2772 6808
Fragments arranged by size:
6808 2772 1410 1251 672 336 276 150
138 105 27
SalI G'TCGA_C
Cuts at: 1936 11287 1936
Size: 9351 4594
SanDI GG'GwC_CC
Cuts at: 10886 10886
Size: 13945
SapI GCTCTTCn'nnn_
Cuts at: 8769 9022 8769
Size: 253 13692
SbfI CC_TGCA'GG
Cuts at: 11297 11297
Size: 13945
ScaI AGT'ACT
Cuts at: 7531 12193 13905 7531
Size: 4662 1712 7571
Fragments arranged by size:
7571 4662 1712
SexAI A'CCwGG_T
Cuts at: 10377 10377
Size: 13945
SfcI C'TryA_G
Cuts at: 826 1045 3324 3780 3843 4164 4566 4950 5001
Size: 219 2279 456 63 321 402 384 51
Cuts at: 5001 6462 6894 8103 10016 10203 10440 11293 12428
Size: 1461 432 1209 1913 187 237 853 1135
Cuts at: 12428 13109 13300 826
Size: 681 191 1471
Fragments arranged by size:
2279 1913 1471 1461 1209 1135 853 681
456 432 402 384 321 237 219 191
187 63 51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13945
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 13945
SmaI CCC'GGG
Cuts at: 1872 5870 11272 1872
Size: 3998 5402 4545
Fragments arranged by size:
5402 4545 3998
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2206 2495 5529 6037 11129 12049
Size: 197 926 263 289 3034 508 5092 920
Cuts at: 12049 12950 13212 820
Size: 901 262 1553
Fragments arranged by size:
5092 3034 1553 926 920 901 508 289
263 262 197
SnaBI TAC'GTA
Cuts at: 493 11736 493
Size: 11243 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 13945
SphI G_CATG'C
Cuts at: 1132 2178 11303 1132
Size: 1046 9125 3774
Fragments arranged by size:
9125 3774 1046
SspI AAT'ATT
Cuts at: 5 52 4088 7297 9385 11869 5
Size: 47 4036 3209 2088 2484 2081
Fragments arranged by size:
4036 3209 2484 2088 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 13945
StyI C'CwwG_G
Cuts at: 513 1065 2272 2482 3081 3344 3563 3897 4205
Size: 552 1207 210 599 263 219 334 308
Cuts at: 4205 4337 4763 5245 5304 7469 7849 8780 9787
Size: 132 426 482 59 2165 380 931 1007
Cuts at: 9787 11384 513
Size: 1597 3074
Fragments arranged by size:
3074 2165 1597 1207 1007 931 599 552
482 426 380 334 308 263 219 210
132 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3845 4892 8032 8172 8225 10082 10184
Size: 1255 2265 1047 3140 140 53 1857 102
Cuts at: 10184 11004 11972 11989 12142 12327 12772 325
Size: 820 968 17 153 185 445 1498
Fragments arranged by size:
3140 2265 1857 1498 1255 1047 968 820
445 185 153 140 102 53 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2550 2723 3715
Size: 274 80 33 51 1443 573 173 992
Cuts at: 3715 5719 6659 7529 8301 12191 13903 96
Size: 2004 940 870 772 3890 1712 138
Fragments arranged by size:
3890 2004 1712 1443 992 940 870 772
573 274 173 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3421 3453 3697 3729 6372 6404 10272 10304 13905
Size: 32 244 32 2643 32 3868 32 3601
Cuts at: 13905 13937 3421
Size: 32 3429
Fragments arranged by size:
3868 3601 3429 2643 244 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 8362 8395 1316
Size: 7046 33 6866
Fragments arranged by size:
7046 6866 33
XbaI T'CTAG_A
Cuts at: 11281 11281
Size: 13945
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6991 7579 9728 10204 6991
Size: 588 2149 476 10732
Fragments arranged by size:
10732 2149 588 476
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 13945
XmaI C'CCGG_G
Cuts at: 1870 5868 11270 1870
Size: 3998 5402 4545
Fragments arranged by size:
5402 4545 3998
XmnI GAAnn'nnTTC
Cuts at: 3096 10901 12074 3096
Size: 7805 1173 4967
Fragments arranged by size:
7805 4967 1173
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2638 11753 276
Size: 53 83 186 936 1104 9115 2468
Fragments arranged by size:
9115 2468 1104 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BbeI BsiWI BsmBI BstZ17I EcoICRI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI RsrII SacI SacII SfiI SfoI
SrfI SwaI