Restriction map for
pFN21AE1592
(Circular) (Six-base) MAPSORT of: pFN21AE1592.seq Check: 4679 from: 1 to: 5917
pFN21AE1592 5917 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3727 278
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3260 1937
Size: 1323 4594
Acc65I G'GTAC_C
Cuts at: 3238 3238
Size: 5917
AclI AA'CG_TT
Cuts at: 1563 4044 4417 1563
Size: 2481 373 3063
Fragments arranged by size:
3063 2481 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2042 3980 5364 5632 1632
Size: 410 1938 1384 268 1917
Fragments arranged by size:
1938 1917 1384 410 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5720
AflIII A'CryG_T
Cuts at: 1203 2489 2793 3214 4816 5793 1203
Size: 1286 304 421 1602 977 1327
Fragments arranged by size:
1602 1327 1286 977 421 304
AgeI A'CCGG_T
Cuts at: 4748 4748
Size: 5917
AhdI GACnn_n'nnGTC
Cuts at: 4646 4646
Size: 5917
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5917
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2224 2591 3099 1184
Size: 1040 367 508 4002
Fragments arranged by size:
4002 1040 508 367
AlwNI CAG_nnn'CTG
Cuts at: 3660 5232 3660
Size: 1572 4345
ApaLI G'TGCA_C
Cuts at: 3974 5130 3974
Size: 1156 4761
ApoI r'AATT_y
Cuts at: 1493 2214 2519 3108 3226 3452 3604 5713 1493
Size: 721 305 589 118 226 152 2109 1697
Fragments arranged by size:
2109 1697 721 589 305 226 152 118
AseI AT'TA_AT
Cuts at: 160 1032 4471 160
Size: 872 3439 1606
Fragments arranged by size:
3439 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
AvaI C'yCGr_G
Cuts at: 1870 1943 2166 3242 1870
Size: 73 223 1076 4545
Fragments arranged by size:
4545 1076 223 73
BamHI G'GATC_C
Cuts at: 3247 3614 3247
Size: 367 5550
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3238 4693 618
Size: 325 217 627 1451 1455 1842
Fragments arranged by size:
1842 1455 1451 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3044 3236 1391
Size: 1653 192 4072
Fragments arranged by size:
4072 1653 192
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2247 3064 928
Size: 971 348 817 3781
Fragments arranged by size:
3781 971 817 348
BbvCI CC'TCA_GC
Cuts at: 2550 2550
Size: 5917
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4108 4142 1903
Size: 34 2171 34 3678
Fragments arranged by size:
3678 2171 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3809 5019 3809
Size: 1210 4707
BclI T'GATC_A
Cuts at: 1548 2841 1548
Size: 1293 4624
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4528 136
Size: 107 122 71 631 605 2856 1525
Fragments arranged by size:
2856 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5912 5912
Size: 5917
BlpI GC'TnA_GC
Cuts at: 3334 3334
Size: 5917
Bme1580I G_kGCm'C
Cuts at: 1790 2203 3978 5134 1790
Size: 413 1775 1156 2573
Fragments arranged by size:
2573 1775 1156 413
BmgBI CAC'GTC
Cuts at: 1318 2492 1318
Size: 1174 4743
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3648 4606 442
Size: 416 53 83 399 24 2231 958 1753
Fragments arranged by size:
2231 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2565 2669 4577 1239
Size: 1326 104 1908 2579
Fragments arranged by size:
2579 1908 1326 104
Bpu10I CC'TnA_GC
Cuts at: 2550 2550
Size: 5917
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2301 4042 4907 5205 2301
Size: 1741 865 298 3013
Fragments arranged by size:
3013 1741 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4580 882
Size: 617 3081 2219
Fragments arranged by size:
3081 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3708 493
Size: 711 2504 2702
Fragments arranged by size:
2702 2504 711
BsaBI GATnn'nnATC
Cuts at: 2846 5911 2846
Size: 3065 2852
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3724 4106 4741 275
Size: 53 83 186 936 2191 382 635 1451
Fragments arranged by size:
2191 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4350 4748 5022 5169 5580 4350
Size: 398 274 147 411 4687
Fragments arranged by size:
4687 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2538 2568 1178
Size: 30 165 30 1135 30 4527
Fragments arranged by size:
4527 1135 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2334 1186
Size: 1148 4769
BseYI C'CCAG_C
Cuts at: 2263 2365 5120 5616 2263
Size: 102 2755 496 2564
Fragments arranged by size:
2755 2564 496 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2375 1163
Size: 573 639 4705
Fragments arranged by size:
4705 639 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3367 4128 5156 1540
Size: 275 186 1366 761 1028 2301
Fragments arranged by size:
2301 1366 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2676 3978 4063 5134 2676
Size: 1302 85 1071 3459
Fragments arranged by size:
3459 1302 1071 85
BsmI GAATG_Cn'
Cuts at: 3428 3521 3428
Size: 93 5824
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2203 2676 3044 3236 3978 4063 5134
Size: 399 413 473 368 192 742 85 1071
Cuts at: 5134 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 473 413 399 368 192
85
BspEI T'CCGG_A
Cuts at: 5580 5580
Size: 5917
BspHI T'CATG_A
Cuts at: 3806 5536 3806
Size: 1730 4187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3272 844
Size: 2428 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3804 1394
Size: 429 1981 3507
Fragments arranged by size:
3507 1981 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2518 4412 4586 65
Size: 1183 1270 1894 174 1396
Fragments arranged by size:
1894 1396 1270 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4561 4748 1700
Size: 110 2751 187 2869
Fragments arranged by size:
2869 2751 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5917
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5917
BssSI C'ACGA_G
Cuts at: 3977 4989 3977
Size: 1012 4905
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5917
BstBI TT'CG_AA
Cuts at: 4721 4721
Size: 5917
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5917
BstYI r'GATC_y
Cuts at: 1972 3247 3614 3999 4016 5468 5554 5566 5912
Size: 1275 367 385 17 1452 86 12 346
Cuts at: 5912 1972
Size: 1977
Fragments arranged by size:
1977 1452 1275 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2346 2383 2346
Size: 37 5880
BtgI C'CryG_G
Cuts at: 513 1065 2004 2052 2860 513
Size: 552 939 48 808 3570
Fragments arranged by size:
3570 939 808 552 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5178
Fragments arranged by size:
5178 683 56
BtsI GCAGTG_nn'
Cuts at: 2358 2622 3433 4227 4247 2358
Size: 264 811 794 20 4028
Fragments arranged by size:
4028 811 794 264 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5917
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5882
DraI TTT'AAA
Cuts at: 2890 3221 2890
Size: 331 5586
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4924 809
Size: 507 3608 1802
Fragments arranged by size:
3608 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3364 4253
Size: 54 1425 50 275 18 177 1357 889
Cuts at: 4253 5882 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1357 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3364 1537
Size: 275 1552 4090
Fragments arranged by size:
4090 1552 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2024 2112 2931 3004 3209 3847 1353
Size: 671 88 819 73 205 638 3423
Fragments arranged by size:
3423 819 671 638 205 88 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4504 4878 5024 387
Size: 1064 138 2915 374 146 1280
Fragments arranged by size:
2915 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2565 2669 3980 4577 5364 5632
Size: 393 410 523 104 1311 597 787 268
Cuts at: 5632 1239
Size: 1524
Fragments arranged by size:
1524 1311 787 597 523 410 393 268
104
EcoO109I rG'GnC_Cy
Cuts at: 2017 2017
Size: 5917
EcoRI G'AATT_C
Cuts at: 3226 3604 3226
Size: 378 5539
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2342 3198 2342
Size: 856 5061
FspI TGC'GCA
Cuts at: 4423 4423
Size: 5917
HaeII r_GCGC'y
Cuts at: 1678 5064 1678
Size: 3386 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2270 2302 4558 4590 4632
Size: 32 135 32 698 32 2256 32 42
Cuts at: 4632 4664 1373
Size: 32 2626
Fragments arranged by size:
2626 2256 698 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2557 3261 3507 669
Size: 988 281 619 704 246 3079
Fragments arranged by size:
3079 988 704 619 281 246
HindIII A'AGCT_T
Cuts at: 748 2405 748
Size: 1657 4260
HpaI GTT'AAC
Cuts at: 3507 3507
Size: 5917
KpnI G_GTAC'C
Cuts at: 3242 3242
Size: 5917
MfeI C'AATT_G
Cuts at: 3516 3516
Size: 5917
MluI A'CGCG_T
Cuts at: 5793 5793
Size: 5917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5031 5215 1305
Size: 3726 184 2007
Fragments arranged by size:
3726 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4095
Fragments arranged by size:
4095 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2206 2567 2573 3875 4234 4393 518
Size: 648 1040 361 6 1302 359 159 2042
Fragments arranged by size:
2042 1302 1040 648 361 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 3333 4010 5158 5403 1606
Size: 1727 677 1148 245 2120
Fragments arranged by size:
2120 1727 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2052 513
Size: 552 939 48 4378
Fragments arranged by size:
4378 939 552 48
NdeI CA'TA_TG
Cuts at: 387 3072 387
Size: 2685 3232
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5917
NotI GC'GGCC_GC
Cuts at: 3364 3364
Size: 5917
NspI r_CATG'y
Cuts at: 1132 1229 2211 2797 3218 3275 4820 1132
Size: 97 982 586 421 57 1545 2229
Fragments arranged by size:
2229 1545 982 586 421 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5917
PciI A'CATG_T
Cuts at: 2793 3214 4816 2793
Size: 421 1602 3894
Fragments arranged by size:
3894 1602 421
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5917
PmeI GTTT'AAAC
Cuts at: 3221 3221
Size: 5917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3986 4018 5499 5531 3986
Size: 32 1481 32 4372
Fragments arranged by size:
4372 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2017 2017
Size: 5917
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5917
PsiI TTA'TAA
Cuts at: 3487 3487
Size: 5917
PstI C_TGCA'G
Cuts at: 830 3269 830
Size: 2439 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5917
SalI G'TCGA_C
Cuts at: 1936 3259 1936
Size: 1323 4594
SapI GCTCTTCn'nnn_
Cuts at: 3004 3004
Size: 5917
SbfI CC_TGCA'GG
Cuts at: 3269 3269
Size: 5917
ScaI AGT'ACT
Cuts at: 2325 4165 5877 2325
Size: 1840 1712 2365
Fragments arranged by size:
2365 1840 1712
SfcI C'TryA_G
Cuts at: 826 1045 2148 2640 2931 3265 4400 5081 5272
Size: 219 1103 492 291 334 1135 681 191
Cuts at: 5272 826
Size: 1471
Fragments arranged by size:
1471 1135 1103 681 492 334 291 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5917
SmaI CCC'GGG
Cuts at: 1872 3244 1872
Size: 1372 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2316 4021 4922 5184 820
Size: 197 926 373 1705 901 262 1553
Fragments arranged by size:
1705 1553 926 901 373 262 197
SnaBI TAC'GTA
Cuts at: 493 3708 493
Size: 3215 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5917
SphI G_CATG'C
Cuts at: 1132 2211 3275 1132
Size: 1079 1064 3774
Fragments arranged by size:
3774 1079 1064
SspI AAT'ATT
Cuts at: 5 52 3841 5
Size: 47 3789 2081
Fragments arranged by size:
3789 2081 47
StuI AGG'CCT
Cuts at: 1517 2855 1517
Size: 1338 4579
StyI C'CwwG_G
Cuts at: 513 1065 2004 2052 3356 513
Size: 552 939 48 1304 3074
Fragments arranged by size:
3074 1304 939 552 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2045 3944 3961 4114 4299 4744 325
Size: 1255 465 1899 17 153 185 445 1498
Fragments arranged by size:
1899 1498 1255 465 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2323 4163 5875
Size: 274 80 33 51 1443 346 1840 1712
Cuts at: 5875 96
Size: 138
Fragments arranged by size:
1840 1712 1443 346 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5877 5909 5877
Size: 32 5885
Tth111I GACn'n_nGTC
Cuts at: 1316 2015 1316
Size: 699 5218
XbaI T'CTAG_A
Cuts at: 3253 3253
Size: 5917
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2858 2858
Size: 5917
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5917
XmaI C'CCGG_G
Cuts at: 1870 3242 1870
Size: 1372 4545
XmnI GAAnn'nnTTC
Cuts at: 4046 4046
Size: 5917
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3725 276
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI