Restriction map for
pFN21AE1591
(Circular) (Six-base) MAPSORT of: pFN21AE1591.seq Check: 3817 from: 1 to: 6091
pFN21AE1591 6091 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3901 278
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3087 3434 1937
Size: 1150 347 4594
Fragments arranged by size:
4594 1150 347
Acc65I G'GTAC_C
Cuts at: 2058 3412 2058
Size: 1354 4737
AclI AA'CG_TT
Cuts at: 1563 4218 4591 1563
Size: 2655 373 3063
Fragments arranged by size:
3063 2655 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2229 3218 4154 5538 5806 1632
Size: 597 989 936 1384 268 1917
Fragments arranged by size:
1917 1384 989 936 597 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 2370 2372 2653 3120 4990 5967 1203
Size: 1167 2 281 467 1870 977 1327
Fragments arranged by size:
1870 1327 1167 977 467 281 2
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 4820 4820
Size: 6091
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6091
AlwNI CAG_nnn'CTG
Cuts at: 2025 2316 2953 3028 3834 5406 2025
Size: 291 637 75 806 1572 2710
Fragments arranged by size:
2710 1572 806 637 291 75
ApaLI G'TGCA_C
Cuts at: 4148 5304 4148
Size: 1156 4935
ApoI r'AATT_y
Cuts at: 1493 3400 3626 3778 5887 1493
Size: 1907 226 152 2109 1697
Fragments arranged by size:
2109 1907 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4645 160
Size: 872 3613 1606
Fragments arranged by size:
3613 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 2519 2840 3211 3416 1870
Size: 73 576 321 371 205 4545
Fragments arranged by size:
4545 576 371 321 205 73
BamHI G'GATC_C
Cuts at: 3421 3788 3421
Size: 367 5724
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2046 2058 2065 2509 3412
Size: 325 217 627 259 12 7 444 903
Cuts at: 3412 4867 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 903 627 444 325 259 217
12 7
BanII G_rGCy'C
Cuts at: 1391 2565 3218 3410 1391
Size: 1174 653 192 4072
Fragments arranged by size:
4072 1174 653 192
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5120
BbvCI CC'TCA_GC
Cuts at: 3347 3347
Size: 6091
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4282 4316 1903
Size: 34 2345 34 3678
Fragments arranged by size:
3678 2345 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3983 5193 3983
Size: 1210 4881
BclI T'GATC_A
Cuts at: 1548 3189 1548
Size: 1641 4450
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2619 3176 4702
Size: 107 122 71 631 605 947 557 1526
Cuts at: 4702 136
Size: 1525
Fragments arranged by size:
1526 1525 947 631 605 557 122 107
71
BglII A'GATC_T
Cuts at: 6086 6086
Size: 6091
BlpI GC'TnA_GC
Cuts at: 2990 3180 3508 2990
Size: 190 328 5573
Fragments arranged by size:
5573 328 190
Bme1580I G_kGCm'C
Cuts at: 1790 2049 2223 2514 4152 5308 1790
Size: 259 174 291 1638 1156 2573
Fragments arranged by size:
2573 1638 1156 291 259 174
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6091
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3822 4780 442
Size: 416 53 83 399 24 2405 958 1753
Fragments arranged by size:
2405 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2120 2981 3050 3092 4751 1239
Size: 881 861 69 42 1659 2579
Fragments arranged by size:
2579 1659 881 861 69 42
Bpu10I CC'TnA_GC
Cuts at: 3347 3347
Size: 6091
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2771 4216 5081 5379 2771
Size: 1445 865 298 3483
Fragments arranged by size:
3483 1445 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2183 3009 4754 882
Size: 617 684 826 1745 2219
Fragments arranged by size:
2219 1745 826 684 617
BsaAI yAC'GTr
Cuts at: 493 1204 2373 3091 3882 493
Size: 711 1169 718 791 2702
Fragments arranged by size:
2702 1169 791 718 711
BsaBI GATnn'nnATC
Cuts at: 3206 6085 3206
Size: 2879 3212
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2546 3898 4280 4915
Size: 53 83 186 936 1013 1352 382 635
Cuts at: 4915 275
Size: 1451
Fragments arranged by size:
1451 1352 1013 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2883 4524 4922 5196 5343 5754 2883
Size: 1641 398 274 147 411 3220
Fragments arranged by size:
3220 1641 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2006 2036 1178
Size: 30 165 30 603 30 5233
Fragments arranged by size:
5233 603 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2774 1186
Size: 1588 4503
BseYI C'CCAG_C
Cuts at: 2098 2176 2222 2617 3107 3341 3353 5294 5790
Size: 78 46 395 490 234 12 1941 496
Cuts at: 5790 2098
Size: 2399
Fragments arranged by size:
2399 1941 496 490 395 234 78 46
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2809 1163
Size: 573 1073 4445
Fragments arranged by size:
4445 1073 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2488 3541 4302 5330 1540
Size: 275 186 487 1053 761 1028 2301
Fragments arranged by size:
2301 1053 1028 761 487 275 186
BsiHKAI G_wGCw'C
Cuts at: 2528 2565 4152 4237 5308 2528
Size: 37 1587 85 1071 3311
Fragments arranged by size:
3311 1587 1071 85 37
BsmI GAATG_Cn'
Cuts at: 3602 3695 3602
Size: 93 5998
BsmBI CGTCTCn'nnnn_
Cuts at: 2972 2972
Size: 6091
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2223 2514 2528 2565 3218 3410
Size: 399 259 174 291 14 37 653 192
Cuts at: 3410 4152 4237 5308 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 653 399 291 259 192
174 85 37 14
BspEI T'CCGG_A
Cuts at: 5754 5754
Size: 6091
BspHI T'CATG_A
Cuts at: 3980 5710 3980
Size: 1730 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3162 3446 844
Size: 2318 284 3489
Fragments arranged by size:
3489 2318 284
BsrBI CCG'CTC
Cuts at: 1394 1823 3184 3276 3978 1394
Size: 429 1361 92 702 3507
Fragments arranged by size:
3507 1361 702 429 92
BsrDI GCAATG_nn'
Cuts at: 65 1248 4586 4760 65
Size: 1183 3338 174 1396
Fragments arranged by size:
3338 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4735 4922 1700
Size: 110 2925 187 2869
Fragments arranged by size:
2925 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2367 96
Size: 2271 3820
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6091
BssSI C'ACGA_G
Cuts at: 4151 5163 4151
Size: 1012 5079
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2025 794
Size: 1231 4860
BstBI TT'CG_AA
Cuts at: 4895 4895
Size: 6091
BstEII G'GTnAC_C
Cuts at: 1184 2407 1184
Size: 1223 4868
BstYI r'GATC_y
Cuts at: 1972 2820 3207 3421 3788 4173 4190 5642 5728
Size: 848 387 214 367 385 17 1452 86
Cuts at: 5728 5740 6086 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 848 387 385 367 346 214
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2290 2290
Size: 6091
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4600
Fragments arranged by size:
4600 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2749 487
Size: 683 56 1523 3829
Fragments arranged by size:
3829 1523 683 56
BtsI GCAGTG_nn'
Cuts at: 3607 4401 4421 3607
Size: 794 20 5277
Fragments arranged by size:
5277 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6056
DraI TTT'AAA
Cuts at: 3395 3395
Size: 6091
DraIII CAC_nnn'GTG
Cuts at: 2706 2706
Size: 6091
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5098 809
Size: 507 3782 1802
Fragments arranged by size:
3782 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3375 3538
Size: 54 1425 50 275 18 177 1368 163
Cuts at: 3538 4427 6056 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1368 889 275 177 163 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3538 1537
Size: 275 1726 4090
Fragments arranged by size:
4090 1726 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2205 4021 1353
Size: 852 1816 3423
Fragments arranged by size:
3423 1816 852
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2330 4678 5052 5198 387
Size: 1064 138 741 2348 374 146 1280
Fragments arranged by size:
2348 1280 1064 741 374 146 138
EcoICRI GAG'CTC
Cuts at: 2563 2563
Size: 6091
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 2229 2981 3050 3092 3218 4154
Size: 393 488 109 752 69 42 126 936
Cuts at: 4154 4751 5538 5806 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 936 787 752 597 488 393 268
126 109 69 42
EcoNI CCTnn'n_nnAGG
Cuts at: 2786 2786
Size: 6091
EcoO109I rG'GnC_Cy
Cuts at: 3025 3259 3025
Size: 234 5857
EcoRI G'AATT_C
Cuts at: 3400 3778 3400
Size: 378 5713
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2284 2353 2284
Size: 69 6022
FspI TGC'GCA
Cuts at: 2579 4597 2579
Size: 2018 4073
HaeII r_GCGC'y
Cuts at: 1678 2124 2316 2997 3080 3171 5238 1678
Size: 446 192 681 83 91 2067 2531
Fragments arranged by size:
2531 2067 681 446 192 91 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2273 2305 4732 4764 4806
Size: 32 135 32 701 32 2427 32 42
Cuts at: 4806 4838 1373
Size: 32 2626
Fragments arranged by size:
2626 2427 701 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3435 3681 669
Size: 988 281 1497 246 3079
Fragments arranged by size:
3079 1497 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6091
HpaI GTT'AAC
Cuts at: 3681 3681
Size: 6091
KpnI G_GTAC'C
Cuts at: 2062 3416 2062
Size: 1354 4737
MfeI C'AATT_G
Cuts at: 3690 3690
Size: 6091
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3225 3384 5205 5389 1305
Size: 1920 159 1821 184 2007
Fragments arranged by size:
2007 1920 1821 184 159
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 4092
Fragments arranged by size:
4092 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2052 4049 4408 4567 518
Size: 648 886 1997 359 159 2042
Fragments arranged by size:
2042 1997 886 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2421 2617 2740 2950 3067 3333 3507 4184
Size: 815 196 123 210 117 266 174 677
Cuts at: 4184 5332 5577 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 815 677 266 245 210 196
174 123 117
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4600
Fragments arranged by size:
4600 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6091
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6091
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 2464 3055 3124 3449 4994 1132
Size: 97 1235 591 69 325 1545 2229
Fragments arranged by size:
2229 1545 1235 591 325 97 69
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6091
PciI A'CATG_T
Cuts at: 3120 4990 3120
Size: 1870 4221
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2156 3028 1108
Size: 1048 872 4171
Fragments arranged by size:
4171 1048 872
PfoI T'CCnGG_A
Cuts at: 3234 3234
Size: 6091
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PmlI CAC'GTG
Cuts at: 2373 2373
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2188 2220 2627 2659 4160 4192 5673 5705 2188
Size: 32 407 32 1501 32 1481 32 2574
Fragments arranged by size:
2574 1501 1481 407 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3025 3025
Size: 6091
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6091
PsiI TTA'TAA
Cuts at: 3661 3661
Size: 6091
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2351 2383 2351
Size: 32 6059
PstI C_TGCA'G
Cuts at: 830 2442 3060 3443 830
Size: 1612 618 383 3478
Fragments arranged by size:
3478 1612 618 383
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
PvuII CAG'CTG
Cuts at: 2617 2740 2950 3333 2617
Size: 123 210 383 5375
Fragments arranged by size:
5375 383 210 123
RsrII CG'GwC_CG
Cuts at: 3040 3040
Size: 6091
SacI G_AGCT'C
Cuts at: 2565 2565
Size: 6091
SalI G'TCGA_C
Cuts at: 1936 3433 1936
Size: 1497 4594
SbfI CC_TGCA'GG
Cuts at: 3443 3443
Size: 6091
ScaI AGT'ACT
Cuts at: 2429 4339 6051 2429
Size: 1910 1712 2469
Fragments arranged by size:
2469 1910 1712
SexAI A'CCwGG_T
Cuts at: 2925 2925
Size: 6091
SfcI C'TryA_G
Cuts at: 826 1045 2438 3056 3334 3439 4574 5255 5446
Size: 219 1393 618 278 105 1135 681 191
Cuts at: 5446 826
Size: 1471
Fragments arranged by size:
1471 1393 1135 681 618 278 219 191
105
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 3213 3418 1872
Size: 1341 205 4545
Fragments arranged by size:
4545 1341 205
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2786 4195 5096 5358 820
Size: 197 926 843 1409 901 262 1553
Fragments arranged by size:
1553 1409 926 901 843 262 197
SnaBI TAC'GTA
Cuts at: 493 3882 493
Size: 3389 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6091
SphI G_CATG'C
Cuts at: 1132 3449 1132
Size: 2317 3774
SspI AAT'ATT
Cuts at: 5 52 4015 5
Size: 47 3963 2081
Fragments arranged by size:
3963 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6091
StyI C'CwwG_G
Cuts at: 513 1065 2004 2466 3171 3530 513
Size: 552 939 462 705 359 3074
Fragments arranged by size:
3074 939 705 552 462 359
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2090 2502 3395 4118 4135 4288 4473
Size: 1255 510 412 893 723 17 153 185
Cuts at: 4473 4918 325
Size: 445 1498
Fragments arranged by size:
1498 1255 893 723 510 445 412 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2367 2427 4337
Size: 274 80 33 51 1443 390 60 1910
Cuts at: 4337 6049 96
Size: 1712 138
Fragments arranged by size:
1910 1712 1443 390 274 138 80 60
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6051 6083 6051
Size: 32 6059
Tth111I GACn'n_nGTC
Cuts at: 1316 2927 1316
Size: 1611 4480
XbaI T'CTAG_A
Cuts at: 3427 3427
Size: 6091
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 3211 3416 1870
Size: 1341 205 4545
Fragments arranged by size:
4545 1341 205
XmnI GAAnn'nnTTC
Cuts at: 4220 4220
Size: 6091
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3899 276
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
PvuII RsrII SacI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstXI BstZ17I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI
PspXI SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI