Restriction map for
pFN21AE1590
(Circular) (Six-base) MAPSORT of: pFN21AE1590.seq Check: 6443 from: 1 to: 8209
pFN21AE1590 8209 bp
With 182 enzymes: SgfI *
February 16, 2010 11:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5299 5299
Size: 8209
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6019 278
Size: 53 83 186 936 4483 2468
Fragments arranged by size:
4483 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5552 1937
Size: 3615 4594
Acc65I G'GTAC_C
Cuts at: 5530 5530
Size: 8209
AclI AA'CG_TT
Cuts at: 1563 6336 6709 1563
Size: 4773 373 3063
Fragments arranged by size:
4773 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2770 2919 3757 4292 4964 5399 6272 7656
Size: 1138 149 838 535 672 435 873 1384
Cuts at: 7656 7924 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1138 873 838 672 535 435
268 149
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8012
AflIII A'CryG_T
Cuts at: 1203 2505 2986 3054 7108 8085 1203
Size: 1302 481 68 4054 977 1327
Fragments arranged by size:
4054 1327 1302 977 481 68
AgeI A'CCGG_T
Cuts at: 7040 7040
Size: 8209
AhdI GACnn_n'nnGTC
Cuts at: 6938 6938
Size: 8209
AleI CACnn'nnGTG
Cuts at: 1166 4422 1166
Size: 3256 4953
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2714 1184
Size: 1530 6679
AlwNI CAG_nnn'CTG
Cuts at: 4201 5952 7524 4201
Size: 1751 1572 4886
Fragments arranged by size:
4886 1751 1572
ApaI G_GGCC'C
Cuts at: 4119 4119
Size: 8209
ApaLI G'TGCA_C
Cuts at: 3064 5482 6266 7422 3064
Size: 2418 784 1156 3851
Fragments arranged by size:
3851 2418 1156 784
ApoI r'AATT_y
Cuts at: 1493 2947 3220 4436 5518 5744 5896 8005 1493
Size: 1454 273 1216 1082 226 152 2109 1697
Fragments arranged by size:
2109 1697 1454 1216 1082 273 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6763 160
Size: 872 5731 1606
Fragments arranged by size:
5731 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8209
AvaI C'yCGr_G
Cuts at: 1870 1943 3543 3837 3847 4825 5492 5534 1870
Size: 73 1600 294 10 978 667 42 4545
Fragments arranged by size:
4545 1600 978 667 294 73 42 10
BamHI G'GATC_C
Cuts at: 2260 5325 5539 5906 2260
Size: 3065 214 367 4563
Fragments arranged by size:
4563 3065 367 214
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3148 5530 6985 618
Size: 325 217 627 1361 2382 1455 1842
Fragments arranged by size:
2382 1842 1455 1361 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2087 2639 3680 4119 4164 5463 5499 5528
Size: 696 552 1041 439 45 1299 36 29
Cuts at: 5528 1391
Size: 4072
Fragments arranged by size:
4072 1299 1041 696 552 439 45 36
29
BbeI G_GCGC'C
Cuts at: 3152 3152
Size: 8209
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2155 2370 3012 3974 928
Size: 971 256 215 642 962 5163
Fragments arranged by size:
5163 971 962 642 256 215
BbvCI CC'TCA_GC
Cuts at: 2139 2298 2139
Size: 159 8050
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6400 6434 1903
Size: 34 4463 34 3678
Fragments arranged by size:
4463 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6101 7311 6101
Size: 1210 6999
BclI T'GATC_A
Cuts at: 1548 2410 1548
Size: 862 7347
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6820 136
Size: 107 122 71 631 605 5148 1525
Fragments arranged by size:
5148 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3509 8204 3509
Size: 4695 3514
BlpI GC'TnA_GC
Cuts at: 4337 4446 5626 4337
Size: 109 1180 6920
Fragments arranged by size:
6920 1180 109
Bme1580I G_kGCm'C
Cuts at: 1790 3068 4119 5486 6270 7426 1790
Size: 1278 1051 1367 784 1156 2573
Fragments arranged by size:
2573 1367 1278 1156 1051 784
BmgBI CAC'GTC
Cuts at: 1318 2611 4412 5191 1318
Size: 1293 1801 779 4336
Fragments arranged by size:
4336 1801 1293 779
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3456 5940 6898
Size: 416 53 83 399 24 2039 2484 958
Cuts at: 6898 442
Size: 1753
Fragments arranged by size:
2484 2039 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8209
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4006 4329 4889 5426 6869 1239
Size: 2767 323 560 537 1443 2579
Fragments arranged by size:
2767 2579 1443 560 537 323
Bpu10I CC'TnA_GC
Cuts at: 2139 2235 2298 3144 5360 2139
Size: 96 63 846 2216 4988
Fragments arranged by size:
4988 2216 846 96 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4727 5357 6334 7199 7497 4727
Size: 630 977 865 298 5439
Fragments arranged by size:
5439 977 865 630 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2059 3307 4685 4845 6872 882
Size: 617 560 1248 1378 160 2027 2219
Fragments arranged by size:
2219 2027 1378 1248 617 560 160
BsaAI yAC'GTr
Cuts at: 493 1204 2989 3055 6000 493
Size: 711 1785 66 2945 2702
Fragments arranged by size:
2945 2702 1785 711 66
BsaBI GATnn'nnATC
Cuts at: 8203 8203
Size: 8209
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3149 3899 6016 6398
Size: 53 83 186 936 1616 750 2117 382
Cuts at: 6398 7033 275
Size: 635 1451
Fragments arranged by size:
2117 1616 1451 936 750 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3023 6642 7040 7314 7461 7872 3023
Size: 3619 398 274 147 411 3360
Fragments arranged by size:
3619 3360 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3695 3725 5465 5495 1178
Size: 30 165 30 2292 30 1740 30 3892
Fragments arranged by size:
3892 2292 1740 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2489 2813 3680 3688 4886 5211 5450 1186
Size: 1303 324 867 8 1198 325 239 3945
Fragments arranged by size:
3945 1303 1198 867 325 324 239 8
BseYI C'CCAG_C
Cuts at: 2715 3156 3762 4066 4475 4533 4596 4889 4942
Size: 441 606 304 409 58 63 293 53
Cuts at: 4942 5462 7412 7908 2715
Size: 520 1950 496 3016
Fragments arranged by size:
3016 1950 606 520 496 441 409 304
293 63 58 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2465 2864 3152 3173 1163
Size: 573 729 399 288 21 6199
Fragments arranged by size:
6199 729 573 399 288 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2542 5414 5470 5659 6420 7448
Size: 275 186 541 2872 56 189 761 1028
Cuts at: 7448 1540
Size: 2301
Fragments arranged by size:
2872 2301 1028 761 541 275 189 186
56
BsiHKAI G_wGCw'C
Cuts at: 2532 3068 3680 3893 4164 4878 5486 6270 6355
Size: 536 612 213 271 714 608 784 85
Cuts at: 6355 7426 2532
Size: 1071 3315
Fragments arranged by size:
3315 1071 784 714 612 608 536 271
213 85
BsmI GAATG_Cn'
Cuts at: 2098 3818 3979 5720 5813 2098
Size: 1720 161 1741 93 4494
Fragments arranged by size:
4494 1741 1720 161 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3890 3890
Size: 8209
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2532 2639 3068 3680 3893 4119
Size: 399 297 445 107 429 612 213 226
Cuts at: 4119 4164 4878 5463 5486 5499 5528 6270 6355
Size: 45 714 585 23 13 29 742 85
Cuts at: 6355 7426 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 714 612 585 445 429
399 297 226 213 107 85 45 29
23 13
BspEI T'CCGG_A
Cuts at: 3023 7872 3023
Size: 4849 3360
BspHI T'CATG_A
Cuts at: 2329 3904 4837 6098 7828 2329
Size: 1575 933 1261 1730 2710
Fragments arranged by size:
2710 1730 1575 1261 933
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3841 5299 5564 844
Size: 2997 1458 265 3489
Fragments arranged by size:
3489 2997 1458 265
BsrBI CCG'CTC
Cuts at: 1394 1823 5410 6096 1394
Size: 429 3587 686 3507
Fragments arranged by size:
3587 3507 686 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6704 6878 65
Size: 1183 5456 174 1396
Fragments arranged by size:
5456 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2376 2572 6853 7040 1700
Size: 110 566 196 4281 187 2869
Fragments arranged by size:
4281 2869 566 196 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8209
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8209
BssSI C'ACGA_G
Cuts at: 4155 4877 6269 7281 4155
Size: 722 1392 1012 5083
Fragments arranged by size:
5083 1392 1012 722
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3187 4405 5389 794
Size: 2393 1218 984 3614
Fragments arranged by size:
3614 2393 1218 984
BstBI TT'CG_AA
Cuts at: 7013 7013
Size: 8209
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8209
BstXI CCAn_nnnn'nTGG
Cuts at: 2684 3586 2684
Size: 902 7307
BstYI r'GATC_y
Cuts at: 1972 2260 3509 3589 4806 5325 5539 5906 6291
Size: 288 1249 80 1217 519 214 367 385
Cuts at: 6291 6308 7760 7846 7858 8204 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1249 1217 519 385 367 346
288 214 86 80 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 5499 513
Size: 552 939 3495 3223
Fragments arranged by size:
3495 3223 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3405 5137 487
Size: 683 56 2179 1732 3559
Fragments arranged by size:
3559 2179 1732 683 56
BtsI GCAGTG_nn'
Cuts at: 2482 3135 5371 5725 6519 6539 2482
Size: 653 2236 354 794 20 4152
Fragments arranged by size:
4152 2236 794 653 354 20
ClaI AT'CG_AT
Cuts at: 1554 5211 1554
Size: 3657 4552
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8174
DraI TTT'AAA
Cuts at: 4735 5002 5513 4735
Size: 267 511 7431
Fragments arranged by size:
7431 511 267
DraIII CAC_nnn'GTG
Cuts at: 3464 3886 3464
Size: 422 7787
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7216 809
Size: 507 5900 1802
Fragments arranged by size:
5900 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2539 2890 3123
Size: 54 1425 50 275 18 709 351 233
Cuts at: 3123 4784 5656 6545 8174 8
Size: 1661 872 889 1629 43
Fragments arranged by size:
1661 1629 1425 889 872 709 351 275
233 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2539 5656 1537
Size: 275 727 3117 4090
Fragments arranged by size:
4090 3117 727 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2537 2885 3291 3369 3735 4719 6139 1353
Size: 1184 348 406 78 366 984 1420 3423
Fragments arranged by size:
3423 1420 1184 984 406 366 348 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2559 5464 6796 7170 7316 387
Size: 1064 138 970 2905 1332 374 146 1280
Fragments arranged by size:
2905 1332 1280 1064 970 374 146 138
EcoICRI GAG'CTC
Cuts at: 3678 4162 3678
Size: 484 7725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2770 2919 3757 4006 4292 4329 4889
Size: 393 1138 149 838 249 286 37 560
Cuts at: 4889 4964 5399 5426 6272 6869 7656 7924 1239
Size: 75 435 27 846 597 787 268 1524
Fragments arranged by size:
1524 1138 846 838 787 597 560 435
393 286 268 249 149 75 37 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2238 3107 3775 2238
Size: 869 668 6672
Fragments arranged by size:
6672 869 668
EcoO109I rG'GnC_Cy
Cuts at: 2764 3706 3843 4116 2764
Size: 942 137 273 6857
Fragments arranged by size:
6857 942 273 137
EcoRI G'AATT_C
Cuts at: 3220 5518 5896 3220
Size: 2298 378 5533
Fragments arranged by size:
5533 2298 378
EcoRV GAT'ATC
Cuts at: 2857 2961 2857
Size: 104 8105
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2216 3676 5222 2216
Size: 1460 1546 5203
Fragments arranged by size:
5203 1546 1460
FspI TGC'GCA
Cuts at: 2702 6715 2702
Size: 4013 4196
HaeII r_GCGC'y
Cuts at: 1678 3152 4270 7356 1678
Size: 1474 1118 3086 2531
Fragments arranged by size:
3086 2531 1474 1118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3695 3727 3908 3940 4413
Size: 32 135 32 2123 32 181 32 473
Cuts at: 4413 4445 5116 5148 6850 6882 6924 6956 1373
Size: 32 671 32 1702 32 42 32 2626
Fragments arranged by size:
2626 2123 1702 671 473 181 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2252 5074 5553 5799 669
Size: 988 281 314 2822 479 246 3079
Fragments arranged by size:
3079 2822 988 479 314 281 246
HindIII A'AGCT_T
Cuts at: 748 5169 748
Size: 4421 3788
HpaI GTT'AAC
Cuts at: 5799 5799
Size: 8209
KasI G'GCGC_C
Cuts at: 3148 3148
Size: 8209
KpnI G_GTAC'C
Cuts at: 5534 5534
Size: 8209
MfeI C'AATT_G
Cuts at: 5808 5808
Size: 8209
MluI A'CGCG_T
Cuts at: 8085 8085
Size: 8209
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7323 7507 1305
Size: 6018 184 2007
Fragments arranged by size:
6018 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2892 10
Size: 54 1425 343 1060 5327
Fragments arranged by size:
5327 1425 1060 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2117 3584 3643 3942 4422 4716 6167
Size: 648 951 1467 59 299 480 294 1451
Cuts at: 6167 6526 6685 518
Size: 359 159 2042
Fragments arranged by size:
2042 1467 1451 951 648 480 359 299
294 159 59
MspA1I CmG'CkG
Cuts at: 1606 2077 2616 2778 3160 3754 4525 4942 5625
Size: 471 539 162 382 594 771 417 683
Cuts at: 5625 6302 7450 7695 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 771 683 677 594 539 471
417 382 245 162
NaeI GCC'GGC
Cuts at: 2574 2574
Size: 8209
NarI GG'CG_CC
Cuts at: 3149 3149
Size: 8209
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6718
Fragments arranged by size:
6718 939 552
NdeI CA'TA_TG
Cuts at: 387 4713 387
Size: 4326 3883
NgoMIV G'CCGG_C
Cuts at: 2572 2572
Size: 8209
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8209
NotI GC'GGCC_GC
Cuts at: 2539 5656 2539
Size: 3117 5092
NspI r_CATG'y
Cuts at: 1132 1229 4544 5567 7112 1132
Size: 97 3315 1023 1545 2229
Fragments arranged by size:
3315 2229 1545 1023 97
PasI CC'CwG_GG
Cuts at: 1348 2127 1348
Size: 779 7430
PciI A'CATG_T
Cuts at: 7108 7108
Size: 8209
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4663 5242 1108
Size: 3555 579 4075
Fragments arranged by size:
4075 3555 579
PfoI T'CCnGG_A
Cuts at: 3983 4345 3983
Size: 362 7847
PmeI GTTT'AAAC
Cuts at: 5513 5513
Size: 8209
PmlI CAC'GTG
Cuts at: 2989 2989
Size: 8209
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6278 6310 7791 7823 6278
Size: 32 1481 32 6664
Fragments arranged by size:
6664 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3706 3843 3706
Size: 137 8072
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8209
PsiI TTA'TAA
Cuts at: 5081 5779 5081
Size: 698 7511
PspOMI G'GGCC_C
Cuts at: 4115 4115
Size: 8209
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4558 4590 4558
Size: 32 8177
PstI C_TGCA'G
Cuts at: 830 2076 2757 3570 5049 5427 5561 830
Size: 1246 681 813 1479 378 134 3478
Fragments arranged by size:
3478 1479 1246 813 681 378 134
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8209
PvuII CAG'CTG
Cuts at: 2077 3160 4525 2077
Size: 1083 1365 5761
Fragments arranged by size:
5761 1365 1083
SacI G_AGCT'C
Cuts at: 3680 4164 3680
Size: 484 7725
SalI G'TCGA_C
Cuts at: 1936 5551 1936
Size: 3615 4594
SanDI GG'GwC_CC
Cuts at: 3843 3843
Size: 8209
SapI GCTCTTCn'nnn_
Cuts at: 2537 2885 3735 2537
Size: 348 850 7011
Fragments arranged by size:
7011 850 348
SbfI CC_TGCA'GG
Cuts at: 3570 5561 3570
Size: 1991 6218
ScaI AGT'ACT
Cuts at: 6457 8169 6457
Size: 1712 6497
SfcI C'TryA_G
Cuts at: 826 1045 2072 2753 3566 4317 5045 5423 5557
Size: 219 1027 681 813 751 728 378 134
Cuts at: 5557 6692 7373 7564 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1027 813 751 728 681 681
378 219 191 134
SfoI GGC'GCC
Cuts at: 3150 3150
Size: 8209
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8209
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8209
SmaI CCC'GGG
Cuts at: 1872 3545 3839 3849 5536 1872
Size: 1673 294 10 1687 4545
Fragments arranged by size:
4545 1687 1673 294 10
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4742 4825 5336 6313 7214 7476
Size: 197 926 2799 83 511 977 901 262
Cuts at: 7476 820
Size: 1553
Fragments arranged by size:
2799 1553 977 926 901 511 262 197
83
SnaBI TAC'GTA
Cuts at: 493 6000 493
Size: 5507 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8209
SphI G_CATG'C
Cuts at: 1132 4544 5567 1132
Size: 3412 1023 3774
Fragments arranged by size:
3774 3412 1023
SspI AAT'ATT
Cuts at: 5 52 2839 3442 6133 5
Size: 47 2787 603 2691 2081
Fragments arranged by size:
2787 2691 2081 603 47
StuI AGG'CCT
Cuts at: 1517 3113 3564 3659 3770 4640 1517
Size: 1596 451 95 111 870 5086
Fragments arranged by size:
5086 1596 870 451 111 95
StyI C'CwwG_G
Cuts at: 513 1065 2004 4558 4675 5101 5648 513
Size: 552 939 2554 117 426 547 3074
Fragments arranged by size:
3074 2554 939 552 547 426 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3331 3475 4596 6236 6253 6406 6591
Size: 1255 1751 144 1121 1640 17 153 185
Cuts at: 6591 7036 325
Size: 445 1498
Fragments arranged by size:
1751 1640 1498 1255 1121 445 185 153
144 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3611 6455 8167
Size: 274 80 33 51 1443 1634 2844 1712
Cuts at: 8167 96
Size: 138
Fragments arranged by size:
2844 1712 1634 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2474 2506 4141 4173 5465 5497 8169 8201 2474
Size: 32 1635 32 1292 32 2672 32 2482
Fragments arranged by size:
2672 2482 1635 1292 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2369 1316
Size: 1053 7156
XbaI T'CTAG_A
Cuts at: 4651 5545 4651
Size: 894 7315
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5104 5104
Size: 8209
XhoI C'TCGA_G
Cuts at: 1943 4825 1943
Size: 2882 5327
XmaI C'CCGG_G
Cuts at: 1870 3543 3837 3847 5534 1870
Size: 1673 294 10 1687 4545
Fragments arranged by size:
4545 1687 1673 294 10
XmnI GAAnn'nnTTC
Cuts at: 3220 3300 6338 3220
Size: 80 3038 5091
Fragments arranged by size:
5091 3038 80
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6017 276
Size: 53 83 186 936 4483 2468
Fragments arranged by size:
4483 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I Bsu36I FseI FspAI NruI NsiI PacI PspXI
RsrII SacII SexAI SfiI SrfI SwaI