Restriction map for
pFN21AE1574
(Circular) (Six-base) MAPSORT of: pFN21AE1574.seq Check: 7610 from: 1 to: 6754
pFN21AE1574 6754 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4564 278
Size: 53 83 186 936 3028 2468
Fragments arranged by size:
3028 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3612 4097 1937
Size: 1675 485 4594
Fragments arranged by size:
4594 1675 485
Acc65I G'GTAC_C
Cuts at: 2208 4075 2208
Size: 1867 4887
AclI AA'CG_TT
Cuts at: 1563 4881 5254 1563
Size: 3318 373 3063
Fragments arranged by size:
3318 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2484 2979 3551 4817 6201 6469 1632
Size: 852 495 572 1266 1384 268 1917
Fragments arranged by size:
1917 1384 1266 852 572 495 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6557
AflIII A'CryG_T
Cuts at: 1203 2145 5653 6630 1203
Size: 942 3508 977 1327
Fragments arranged by size:
3508 1327 977 942
AgeI A'CCGG_T
Cuts at: 5585 5585
Size: 6754
AhdI GACnn_n'nnGTC
Cuts at: 5483 5483
Size: 6754
AleI CACnn'nnGTG
Cuts at: 1166 3723 1166
Size: 2557 4197
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6754
AlwNI CAG_nnn'CTG
Cuts at: 3161 3367 3950 4497 6069 3161
Size: 206 583 547 1572 3846
Fragments arranged by size:
3846 1572 583 547 206
ApaI G_GGCC'C
Cuts at: 2057 2409 3760 2057
Size: 352 1351 5051
Fragments arranged by size:
5051 1351 352
ApaLI G'TGCA_C
Cuts at: 2690 4811 5967 2690
Size: 2121 1156 3477
Fragments arranged by size:
3477 2121 1156
ApoI r'AATT_y
Cuts at: 1493 2593 3253 3301 4063 4289 4441 6550 1493
Size: 1100 660 48 762 226 152 2109 1697
Fragments arranged by size:
2109 1697 1100 762 660 226 152 48
AseI AT'TA_AT
Cuts at: 160 1032 5308 160
Size: 872 4276 1606
Fragments arranged by size:
4276 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6754
AvaI C'yCGr_G
Cuts at: 1870 1943 4079 1870
Size: 73 2136 4545
Fragments arranged by size:
4545 2136 73
BamHI G'GATC_C
Cuts at: 2345 4084 4451 2345
Size: 1739 367 4648
Fragments arranged by size:
4648 1739 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2208 4075 5530 618
Size: 325 217 627 421 1867 1455 1842
Fragments arranged by size:
1867 1842 1455 627 421 325 217
BanII G_rGCy'C
Cuts at: 1391 2057 2409 2799 3652 3760 4073 1391
Size: 666 352 390 853 108 313 4072
Fragments arranged by size:
4072 853 666 390 352 313 108
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3613 928
Size: 971 1714 4069
Fragments arranged by size:
4069 1714 971
BbvCI CC'TCA_GC
Cuts at: 2387 2387
Size: 6754
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2654 2688 4945 4979 1903
Size: 34 717 34 2257 34 3678
Fragments arranged by size:
3678 2257 717 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2900 4646 5856 2900
Size: 1746 1210 3798
Fragments arranged by size:
3798 1746 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6754
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2136 5365 136
Size: 107 122 71 631 605 464 3229 1525
Fragments arranged by size:
3229 1525 631 605 464 122 107 71
BglII A'GATC_T
Cuts at: 3960 6749 3960
Size: 2789 3965
BlpI GC'TnA_GC
Cuts at: 4171 4171
Size: 6754
Bme1580I G_kGCm'C
Cuts at: 1790 2057 2409 2650 2694 3002 3760 4815 5971
Size: 267 352 241 44 308 758 1055 1156
Cuts at: 5971 1790
Size: 2573
Fragments arranged by size:
2573 1156 1055 758 352 308 267 241
44
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6754
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4485 5443 442
Size: 416 53 83 399 24 3068 958 1753
Fragments arranged by size:
3068 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6754
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3014 3029 3891 5414 1239
Size: 1775 15 862 1523 2579
Fragments arranged by size:
2579 1775 1523 862 15
Bpu10I CC'TnA_GC
Cuts at: 2387 2387
Size: 6754
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3977 4879 5744 6042 3977
Size: 902 865 298 4689
Fragments arranged by size:
4689 902 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2321 2759 2910 2981 3584 5417 882
Size: 617 822 438 151 71 603 1833 2219
Fragments arranged by size:
2219 1833 822 617 603 438 151 71
BsaAI yAC'GTr
Cuts at: 493 1204 4545 493
Size: 711 3341 2702
Fragments arranged by size:
3341 2702 711
BsaBI GATnn'nnATC
Cuts at: 6748 6748
Size: 6754
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4561 4943 5578 275
Size: 53 83 186 936 3028 382 635 1451
Fragments arranged by size:
3028 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5187 5585 5859 6006 6417 5187
Size: 398 274 147 411 5524
Fragments arranged by size:
5524 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6529
Fragments arranged by size:
6529 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6754
BseYI C'CCAG_C
Cuts at: 5957 6453 5957
Size: 496 6258
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2177 1163
Size: 573 441 5740
Fragments arranged by size:
5740 573 441
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4204 4965 5993 1540
Size: 275 186 2203 761 1028 2301
Fragments arranged by size:
2301 2203 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2694 4815 4900 5971 2694
Size: 2121 85 1071 3477
Fragments arranged by size:
3477 2121 1071 85
BsmI GAATG_Cn'
Cuts at: 4265 4358 4265
Size: 93 6661
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2409 2650 2694 2799 3002 3652
Size: 399 267 352 241 44 105 203 650
Cuts at: 3652 3760 4073 4815 4900 5971 1391
Size: 108 313 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 650 399 352 313 267
241 203 108 105 85 44
BspEI T'CCGG_A
Cuts at: 6417 6417
Size: 6754
BspHI T'CATG_A
Cuts at: 4643 6373 4643
Size: 1730 5024
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2928 4109 844
Size: 2084 1181 3489
Fragments arranged by size:
3489 2084 1181
BsrBI CCG'CTC
Cuts at: 1394 1823 3229 4641 1394
Size: 429 1406 1412 3507
Fragments arranged by size:
3507 1412 1406 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2494 5249 5423 65
Size: 1183 1246 2755 174 1396
Fragments arranged by size:
2755 1396 1246 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3197 5398 5585 1700
Size: 110 1387 2201 187 2869
Fragments arranged by size:
2869 2201 1387 187 110
BsrGI T'GTAC_A
Cuts at: 96 2364 96
Size: 2268 4486
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6754
BssSI C'ACGA_G
Cuts at: 4814 5826 4814
Size: 1012 5742
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3950 794
Size: 3156 3598
BstBI TT'CG_AA
Cuts at: 5558 5558
Size: 6754
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6754
BstXI CCAn_nnnn'nTGG
Cuts at: 2686 2686
Size: 6754
BstYI r'GATC_y
Cuts at: 1972 2345 2607 2865 3960 4084 4451 4836 4853
Size: 373 262 258 1095 124 367 385 17
Cuts at: 4853 6305 6391 6403 6749 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1095 385 373 367 346 262
258 124 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3921 3921
Size: 6754
BtgI C'CryG_G
Cuts at: 513 1065 2004 3355 513
Size: 552 939 1351 3912
Fragments arranged by size:
3912 1351 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3424 487
Size: 683 56 2198 3817
Fragments arranged by size:
3817 2198 683 56
BtsI GCAGTG_nn'
Cuts at: 2210 4270 5064 5084 2210
Size: 2060 794 20 3880
Fragments arranged by size:
3880 2060 794 20
ClaI AT'CG_AT
Cuts at: 1554 2700 1554
Size: 1146 5608
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3160 3195 3855 3890 529
Size: 35 2596 35 660 35 3393
Fragments arranged by size:
3393 2596 660 35 35 35
DraI TTT'AAA
Cuts at: 4058 4058
Size: 6754
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2480 5761 809
Size: 507 1164 3281 1802
Fragments arranged by size:
3281 1802 1164 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2821 3861 4201
Size: 54 1425 50 275 18 991 1040 340
Cuts at: 4201 5090 6719 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1040 991 889 340 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4201 1537
Size: 275 2389 4090
Fragments arranged by size:
4090 2389 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2018 4684 1353
Size: 665 2666 3423
Fragments arranged by size:
3423 2666 665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5341 5715 5861 387
Size: 1064 138 3752 374 146 1280
Fragments arranged by size:
3752 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2484 2979 3014 3029 3551 3891 4817
Size: 393 852 495 35 15 522 340 926
Cuts at: 4817 5414 6201 6469 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 926 852 787 597 522 495 393
340 268 35 15
EcoO109I rG'GnC_Cy
Cuts at: 2054 2075 2275 2405 3471 3756 3757 2054
Size: 21 200 130 1066 285 1 5051
Fragments arranged by size:
5051 1066 285 200 130 21 1
EcoRI G'AATT_C
Cuts at: 2593 3253 4063 4441 2593
Size: 660 810 378 4906
Fragments arranged by size:
4906 810 660 378
FspI TGC'GCA
Cuts at: 5260 5260
Size: 6754
HaeII r_GCGC'y
Cuts at: 1678 2012 5901 1678
Size: 334 3889 2531
Fragments arranged by size:
3889 2531 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5395 5427 5469 5501 1373
Size: 32 135 32 3823 32 42 32 2626
Fragments arranged by size:
3823 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2494 3234 3829 4098 4344 669
Size: 988 281 556 740 595 269 246 3079
Fragments arranged by size:
3079 988 740 595 556 281 269 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6754
HpaI GTT'AAC
Cuts at: 4344 4344
Size: 6754
KpnI G_GTAC'C
Cuts at: 2212 4079 2212
Size: 1867 4887
MfeI C'AATT_G
Cuts at: 3282 4353 3282
Size: 1071 5683
MluI A'CGCG_T
Cuts at: 6630 6630
Size: 6754
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2323 2622 5868 6052 1305
Size: 1018 299 3246 184 2007
Fragments arranged by size:
3246 2007 1018 299 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3863 10
Size: 54 1425 343 2031 2901
Fragments arranged by size:
2901 2031 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2582 3723 4712 5071 5230 518
Size: 648 1416 1141 989 359 159 2042
Fragments arranged by size:
2042 1416 1141 989 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2098 2362 2462 3024 3334 3435 4170 4847
Size: 492 264 100 562 310 101 735 677
Cuts at: 4847 5995 6240 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 735 677 562 492 310 264
245 101 100
NaeI GCC'GGC
Cuts at: 3199 3199
Size: 6754
NcoI C'CATG_G
Cuts at: 513 1065 2004 3355 513
Size: 552 939 1351 3912
Fragments arranged by size:
3912 1351 939 552
NdeI CA'TA_TG
Cuts at: 387 2526 387
Size: 2139 4615
NgoMIV G'CCGG_C
Cuts at: 3197 3197
Size: 6754
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6754
NotI GC'GGCC_GC
Cuts at: 4201 4201
Size: 6754
NspI r_CATG'y
Cuts at: 1132 1229 3034 3556 4055 4112 5657 1132
Size: 97 1805 522 499 57 1545 2229
Fragments arranged by size:
2229 1805 1545 522 499 97 57
PasI CC'CwG_GG
Cuts at: 1348 2050 2279 2332 1348
Size: 702 229 53 5770
Fragments arranged by size:
5770 702 229 53
PciI A'CATG_T
Cuts at: 5653 5653
Size: 6754
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2530 1108
Size: 1422 5332
PfoI T'CCnGG_A
Cuts at: 2260 2260
Size: 6754
PmeI GTTT'AAAC
Cuts at: 4058 4058
Size: 6754
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4823 4855 6336 6368 4823
Size: 32 1481 32 5209
Fragments arranged by size:
5209 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2075 2275 3471 2075
Size: 200 1196 5358
Fragments arranged by size:
5358 1196 200
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6754
PsiI TTA'TAA
Cuts at: 4324 4324
Size: 6754
PspOMI G'GGCC_C
Cuts at: 2053 2405 3756 2053
Size: 352 1351 5051
Fragments arranged by size:
5051 1351 352
PstI C_TGCA'G
Cuts at: 830 2109 3023 3157 3580 3903 4106 830
Size: 1279 914 134 423 323 203 3478
Fragments arranged by size:
3478 1279 914 423 323 203 134
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6754
PvuII CAG'CTG
Cuts at: 2098 2362 2462 3334 3435 2098
Size: 264 100 872 101 5417
Fragments arranged by size:
5417 872 264 101 100
RsrII CG'GwC_CG
Cuts at: 2371 2371
Size: 6754
SalI G'TCGA_C
Cuts at: 1936 4096 1936
Size: 2160 4594
SanDI GG'GwC_CC
Cuts at: 2075 3471 2075
Size: 1396 5358
SapI GCTCTTCn'nnn_
Cuts at: 2018 2018
Size: 6754
SbfI CC_TGCA'GG
Cuts at: 4106 4106
Size: 6754
ScaI AGT'ACT
Cuts at: 2186 5002 6714 2186
Size: 2816 1712 2226
Fragments arranged by size:
2816 2226 1712
SfcI C'TryA_G
Cuts at: 826 1045 2105 3019 3153 3576 3899 4102 5237
Size: 219 1060 914 134 423 323 203 1135
Cuts at: 5237 5918 6109 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1060 914 681 423 323 219
203 191 134
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6754
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6754
SmaI CCC'GGG
Cuts at: 1872 4081 1872
Size: 2209 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3956 4858 5759 6021 820
Size: 197 926 2013 902 901 262 1553
Fragments arranged by size:
2013 1553 926 902 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4545 493
Size: 4052 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6754
SphI G_CATG'C
Cuts at: 1132 3556 4112 1132
Size: 2424 556 3774
Fragments arranged by size:
3774 2424 556
SspI AAT'ATT
Cuts at: 5 52 4678 5
Size: 47 4626 2081
Fragments arranged by size:
4626 2081 47
StuI AGG'CCT
Cuts at: 1517 2392 2444 2855 1517
Size: 875 52 411 5416
Fragments arranged by size:
5416 875 411 52
StyI C'CwwG_G
Cuts at: 513 1065 2004 3355 3690 3781 4193 513
Size: 552 939 1351 335 91 412 3074
Fragments arranged by size:
3074 1351 939 552 412 335 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4781 4798 4951 5136 5581 325
Size: 1255 3201 17 153 185 445 1498
Fragments arranged by size:
3201 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 2364 2826
Size: 274 80 33 51 1443 207 180 462
Cuts at: 2826 5000 6712 96
Size: 2174 1712 138
Fragments arranged by size:
2174 1712 1443 462 274 207 180 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2209 2241 6714 6746 2209
Size: 32 4473 32 2217
Fragments arranged by size:
4473 2217 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6754
XbaI T'CTAG_A
Cuts at: 4090 4090
Size: 6754
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2621 3871 2621
Size: 1250 5504
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6754
XmaI C'CCGG_G
Cuts at: 1870 4079 1870
Size: 2209 4545
XmnI GAAnn'nnTTC
Cuts at: 2186 4883 2186
Size: 2697 4057
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4562 276
Size: 53 83 186 936 3028 2468
Fragments arranged by size:
3028 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspXI PsrI SacI SacII SexAI SfiI SfoI
SrfI SwaI