Restriction map for
pFN21AE1558
(Circular) (Six-base) MAPSORT of: pFN21AE1558.seq Check: 4807 from: 1 to: 5785
pFN21AE1558 5785 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2032 2032
Size: 5785
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3595 278
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3128 1937
Size: 1191 4594
Acc65I G'GTAC_C
Cuts at: 3106 3106
Size: 5785
AclI AA'CG_TT
Cuts at: 1563 3912 4285 1563
Size: 2349 373 3063
Fragments arranged by size:
3063 2349 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2789 3848 5232 5500 1632
Size: 1157 1059 1384 268 1917
Fragments arranged by size:
1917 1384 1157 1059 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5588
AflIII A'CryG_T
Cuts at: 1203 4684 5661 1203
Size: 3481 977 1327
Fragments arranged by size:
3481 1327 977
AgeI A'CCGG_T
Cuts at: 4616 4616
Size: 5785
AhdI GACnn_n'nnGTC
Cuts at: 4514 4514
Size: 5785
AleI CACnn'nnGTG
Cuts at: 1166 2899 1166
Size: 1733 4052
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5785
AlwNI CAG_nnn'CTG
Cuts at: 2469 3528 5100 2469
Size: 1059 1572 3154
Fragments arranged by size:
3154 1572 1059
ApaLI G'TGCA_C
Cuts at: 3842 4998 3842
Size: 1156 4629
ApoI r'AATT_y
Cuts at: 1493 2775 3094 3320 3472 5581 1493
Size: 1282 319 226 152 2109 1697
Fragments arranged by size:
2109 1697 1282 319 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3024 4339 160
Size: 872 1992 1315 1606
Fragments arranged by size:
1992 1606 1315 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
AvaI C'yCGr_G
Cuts at: 1870 1943 2103 3110 1870
Size: 73 160 1007 4545
Fragments arranged by size:
4545 1007 160 73
BamHI G'GATC_C
Cuts at: 3009 3115 3482 3009
Size: 106 367 5312
Fragments arranged by size:
5312 367 106
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2363 3106 4561 618
Size: 325 217 627 576 743 1455 1842
Fragments arranged by size:
1842 1455 743 627 576 325 217
BanII G_rGCy'C
Cuts at: 1391 2435 2724 3063 3104 1391
Size: 1044 289 339 41 4072
Fragments arranged by size:
4072 1044 339 289 41
BbeI G_GCGC'C
Cuts at: 2367 2367
Size: 5785
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2173 2215 928
Size: 971 274 42 4498
Fragments arranged by size:
4498 971 274 42
BbvCI CC'TCA_GC
Cuts at: 2991 2991
Size: 5785
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3976 4010 1903
Size: 34 2039 34 3678
Fragments arranged by size:
3678 2039 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3677 4887 3677
Size: 1210 4575
BclI T'GATC_A
Cuts at: 1548 3034 1548
Size: 1486 4299
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2294 4396 136
Size: 107 122 71 631 605 622 2102 1525
Fragments arranged by size:
2102 1525 631 622 605 122 107 71
BglII A'GATC_T
Cuts at: 5780 5780
Size: 5785
BlpI GC'TnA_GC
Cuts at: 2394 3202 2394
Size: 808 4977
Bme1580I G_kGCm'C
Cuts at: 1790 3846 5002 1790
Size: 2056 1156 2573
Fragments arranged by size:
2573 2056 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5785
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3516 4474 442
Size: 416 53 83 399 24 2099 958 1753
Fragments arranged by size:
2099 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5785
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2656 2656
Size: 5785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2182 4445 1239
Size: 943 2263 2579
Fragments arranged by size:
2579 2263 943
Bpu10I CC'TnA_GC
Cuts at: 2991 2991
Size: 5785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3910 4775 5073 3910
Size: 865 298 4622
Fragments arranged by size:
4622 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2123 4448 882
Size: 617 624 2325 2219
Fragments arranged by size:
2325 2219 624 617
BsaAI yAC'GTr
Cuts at: 493 1204 3576 493
Size: 711 2372 2702
Fragments arranged by size:
2702 2372 711
BsaBI GATnn'nnATC
Cuts at: 5779 5779
Size: 5785
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2364 3592 3974 4609
Size: 53 83 186 936 831 1228 382 635
Cuts at: 4609 275
Size: 1451
Fragments arranged by size:
1451 1228 936 831 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4218 4616 4890 5037 5448 4218
Size: 398 274 147 411 4555
Fragments arranged by size:
4555 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2075 2105 1178
Size: 30 165 30 672 30 4858
Fragments arranged by size:
4858 672 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 2153 2645 1186
Size: 910 57 492 4326
Fragments arranged by size:
4326 910 492 57
BseYI C'CCAG_C
Cuts at: 2681 4988 5484 2681
Size: 2307 496 2982
Fragments arranged by size:
2982 2307 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2004 1163
Size: 573 268 4944
Fragments arranged by size:
4944 573 268
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3235 3996 5024 1540
Size: 275 186 1234 761 1028 2301
Fragments arranged by size:
2301 1234 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2724 3063 3846 3931 5002 2724
Size: 339 783 85 1071 3507
Fragments arranged by size:
3507 1071 783 339 85
BsmI GAATG_Cn'
Cuts at: 3296 3389 3296
Size: 93 5692
BsmBI CGTCTCn'nnnn_
Cuts at: 2250 2250
Size: 5785
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2435 2724 3063 3104 3846 3931 5002
Size: 399 645 289 339 41 742 85 1071
Cuts at: 5002 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 645 399 339 289 85
41
BspEI T'CCGG_A
Cuts at: 5448 5448
Size: 5785
BspHI T'CATG_A
Cuts at: 2656 3674 5404 2656
Size: 1018 1730 3037
Fragments arranged by size:
3037 1730 1018
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2032 3140 844
Size: 1188 1108 3489
Fragments arranged by size:
3489 1188 1108
BsrBI CCG'CTC
Cuts at: 1394 1823 3672 1394
Size: 429 1849 3507
Fragments arranged by size:
3507 1849 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3037 4280 4454 65
Size: 1183 1789 1243 174 1396
Fragments arranged by size:
1789 1396 1243 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4429 4616 1700
Size: 110 2619 187 2869
Fragments arranged by size:
2869 2619 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5785
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5785
BssSI C'ACGA_G
Cuts at: 3845 4857 3845
Size: 1012 4773
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2303 794
Size: 1509 4276
BstBI TT'CG_AA
Cuts at: 4589 4589
Size: 5785
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5785
BstXI CCAn_nnnn'nTGG
Cuts at: 2545 2545
Size: 5785
BstYI r'GATC_y
Cuts at: 1972 3009 3115 3482 3867 3884 5336 5422 5434
Size: 1037 106 367 385 17 1452 86 12
Cuts at: 5434 5780 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1037 385 367 346 106 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4294
Fragments arranged by size:
4294 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5046
Fragments arranged by size:
5046 683 56
BtsI GCAGTG_nn'
Cuts at: 2237 2313 3301 4095 4115 2237
Size: 76 988 794 20 3907
Fragments arranged by size:
3907 988 794 76 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5785
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5750
DraI TTT'AAA
Cuts at: 2949 3089 2949
Size: 140 5645
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4792 809
Size: 507 3476 1802
Fragments arranged by size:
3476 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3232 4121 5750
Size: 54 1425 50 275 18 1402 889 1629
Cuts at: 5750 8
Size: 43
Fragments arranged by size:
1629 1425 1402 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3232 1537
Size: 275 1420 4090
Fragments arranged by size:
4090 1420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3715 1353
Size: 2362 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2093 4372 4746 4892 387
Size: 1064 138 504 2279 374 146 1280
Fragments arranged by size:
2279 1280 1064 504 374 146 138
EcoICRI GAG'CTC
Cuts at: 2722 3061 2722
Size: 339 5446
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2182 2789 3848 4445 5232 5500 1239
Size: 393 550 607 1059 597 787 268 1524
Fragments arranged by size:
1524 1059 787 607 597 550 393 268
EcoRI G'AATT_C
Cuts at: 3094 3472 3094
Size: 378 5407
FspI TGC'GCA
Cuts at: 4291 4291
Size: 5785
HaeII r_GCGC'y
Cuts at: 1678 2367 4932 1678
Size: 689 2565 2531
Fragments arranged by size:
2565 2531 689
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2097 2129 2591 2623 2875
Size: 32 135 32 525 32 462 32 252
Cuts at: 2875 2907 4426 4458 4500 4532 1373
Size: 32 1519 32 42 32 2626
Fragments arranged by size:
2626 1519 525 462 252 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3129 3375 669
Size: 988 281 1191 246 3079
Fragments arranged by size:
3079 1191 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5785
HpaI GTT'AAC
Cuts at: 3375 3375
Size: 5785
KasI G'GCGC_C
Cuts at: 2363 2363
Size: 5785
KpnI G_GTAC'C
Cuts at: 3110 3110
Size: 5785
MfeI C'AATT_G
Cuts at: 3384 3384
Size: 5785
MluI A'CGCG_T
Cuts at: 5661 5661
Size: 5785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2447 2987 4899 5083 1305
Size: 1142 540 1912 184 2007
Fragments arranged by size:
2007 1912 1142 540 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3963
Fragments arranged by size:
3963 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2655 2661 2899 3743 4102 4261 518
Size: 648 1489 6 238 844 359 159 2042
Fragments arranged by size:
2042 1489 844 648 359 238 159 6
MspA1I CmG'CkG
Cuts at: 1606 2183 2824 3201 3878 5026 5271 1606
Size: 577 641 377 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 641 577 377 245
NarI GG'CG_CC
Cuts at: 2364 2364
Size: 5785
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4294
Fragments arranged by size:
4294 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5785
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5785
NotI GC'GGCC_GC
Cuts at: 3232 3232
Size: 5785
NspI r_CATG'y
Cuts at: 1132 1229 3143 4688 1132
Size: 97 1914 1545 2229
Fragments arranged by size:
2229 1914 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5785
PciI A'CATG_T
Cuts at: 4684 4684
Size: 5785
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5785
PmeI GTTT'AAAC
Cuts at: 3089 3089
Size: 5785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3854 3886 5367 5399 3854
Size: 32 1481 32 4240
Fragments arranged by size:
4240 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2219 1613
Size: 606 5179
PsiI TTA'TAA
Cuts at: 3355 3355
Size: 5785
PspXI vC'TCGA_Gb
Cuts at: 2103 2103
Size: 5785
PstI C_TGCA'G
Cuts at: 830 2029 2521 3137 830
Size: 1199 492 616 3478
Fragments arranged by size:
3478 1199 616 492
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5785
PvuII CAG'CTG
Cuts at: 2183 2824 2183
Size: 641 5144
SacI G_AGCT'C
Cuts at: 2724 3063 2724
Size: 339 5446
SalI G'TCGA_C
Cuts at: 1936 3127 1936
Size: 1191 4594
SbfI CC_TGCA'GG
Cuts at: 3137 3137
Size: 5785
ScaI AGT'ACT
Cuts at: 2409 4033 5745 2409
Size: 1624 1712 2449
Fragments arranged by size:
2449 1712 1624
SfcI C'TryA_G
Cuts at: 826 1045 2025 2517 3133 4268 4949 5140 826
Size: 219 980 492 616 1135 681 191 1471
Fragments arranged by size:
1471 1135 980 681 616 492 219 191
SfoI GGC'GCC
Cuts at: 2365 2365
Size: 5785
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5785
SmaI CCC'GGG
Cuts at: 1872 3112 1872
Size: 1240 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2103 3889 4790 5052 820
Size: 197 926 160 1786 901 262 1553
Fragments arranged by size:
1786 1553 926 901 262 197 160
SnaBI TAC'GTA
Cuts at: 493 3576 493
Size: 3083 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5785
SphI G_CATG'C
Cuts at: 1132 3143 1132
Size: 2011 3774
SspI AAT'ATT
Cuts at: 5 52 3709 5
Size: 47 3657 2081
Fragments arranged by size:
3657 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5785
StyI C'CwwG_G
Cuts at: 513 1065 2004 3224 513
Size: 552 939 1220 3074
Fragments arranged by size:
3074 1220 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3812 3829 3982 4167 4612 325
Size: 1255 2232 17 153 185 445 1498
Fragments arranged by size:
2232 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2310 2407 4031
Size: 274 80 33 51 1443 333 97 1624
Cuts at: 4031 5743 96
Size: 1712 138
Fragments arranged by size:
1712 1624 1443 333 274 138 97 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5745 5777 5745
Size: 32 5753
Tth111I GACn'n_nGTC
Cuts at: 1316 2191 1316
Size: 875 4910
XbaI T'CTAG_A
Cuts at: 3121 3121
Size: 5785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3003 3003
Size: 5785
XhoI C'TCGA_G
Cuts at: 1943 2103 1943
Size: 160 5625
XmaI C'CCGG_G
Cuts at: 1870 3110 1870
Size: 1240 4545
XmnI GAAnn'nnTTC
Cuts at: 2923 3914 2923
Size: 991 4794
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3593 276
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI
BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PsrI RsrII SacII SanDI SapI SexAI
SfiI SrfI SwaI