Restriction map for
pFN21AE1557
(Circular) (Six-base) MAPSORT of: pFN21AE1557.seq Check: 9363 from: 1 to: 5689
pFN21AE1557 5689 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3032 1937
Size: 1095 4594
Acc65I G'GTAC_C
Cuts at: 2826 3010 2826
Size: 184 5505
AclI AA'CG_TT
Cuts at: 1563 3816 4189 1563
Size: 2253 373 3063
Fragments arranged by size:
3063 2253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2839 2946 3752 5136 5404 1632
Size: 1207 107 806 1384 268 1917
Fragments arranged by size:
1917 1384 1207 806 268 107
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 2112 4588 5565 1203
Size: 909 2476 977 1327
Fragments arranged by size:
2476 1327 977 909
AgeI A'CCGG_T
Cuts at: 2009 4520 2009
Size: 2511 3178
AhdI GACnn_n'nnGTC
Cuts at: 4418 4418
Size: 5689
AleI CACnn'nnGTG
Cuts at: 1166 2117 1166
Size: 951 4738
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5689
AlwNI CAG_nnn'CTG
Cuts at: 2092 2441 3432 5004 2092
Size: 349 991 1572 2777
Fragments arranged by size:
2777 1572 991 349
ApaLI G'TGCA_C
Cuts at: 3746 4902 3746
Size: 1156 4533
ApoI r'AATT_y
Cuts at: 1493 2998 3224 3376 5485 1493
Size: 1505 226 152 2109 1697
Fragments arranged by size:
2109 1697 1505 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4243 160
Size: 872 3211 1606
Fragments arranged by size:
3211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 2042 3014 1870
Size: 73 99 972 4545
Fragments arranged by size:
4545 972 99 73
BamHI G'GATC_C
Cuts at: 2374 2788 3019 3386 2374
Size: 414 231 367 4677
Fragments arranged by size:
4677 414 367 231
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2174 2826 3010 4465 618
Size: 325 217 627 387 652 184 1455 1842
Fragments arranged by size:
1842 1455 652 627 387 325 217 184
BanII G_rGCy'C
Cuts at: 1391 3008 1391
Size: 1617 4072
BbeI G_GCGC'C
Cuts at: 2178 2178
Size: 5689
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4718
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3880 3914 1903
Size: 34 1943 34 3678
Fragments arranged by size:
3678 1943 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3581 4791 3581
Size: 1210 4479
BclI T'GATC_A
Cuts at: 1548 2263 1548
Size: 715 4974
BfrBI ATG'CAT
Cuts at: 2248 2248
Size: 5689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4300 136
Size: 107 122 71 631 605 2628 1525
Fragments arranged by size:
2628 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5684 5684
Size: 5689
BlpI GC'TnA_GC
Cuts at: 3106 3106
Size: 5689
Bme1580I G_kGCm'C
Cuts at: 1790 3750 4906 1790
Size: 1960 1156 2573
Fragments arranged by size:
2573 1960 1156
BmgBI CAC'GTC
Cuts at: 1318 2115 1318
Size: 797 4892
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2080 2705 3420
Size: 416 53 83 399 24 663 625 715
Cuts at: 3420 4378 442
Size: 958 1753
Fragments arranged by size:
1753 958 715 663 625 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2236 2463 4349 1239
Size: 997 227 1886 2579
Fragments arranged by size:
2579 1886 997 227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2141 3814 4679 4977 2141
Size: 1673 865 298 2853
Fragments arranged by size:
2853 1673 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2439 4352 882
Size: 617 940 1913 2219
Fragments arranged by size:
2219 1913 940 617
BsaAI yAC'GTr
Cuts at: 493 1204 2512 3480 493
Size: 711 1308 968 2702
Fragments arranged by size:
2702 1308 968 711
BsaBI GATnn'nnATC
Cuts at: 5683 5683
Size: 5689
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2175 3496 3878 4513
Size: 53 83 186 936 642 1321 382 635
Cuts at: 4513 275
Size: 1451
Fragments arranged by size:
1451 1321 936 642 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2009 4122 4520 4794 4941 5352 2009
Size: 2113 398 274 147 411 2346
Fragments arranged by size:
2346 2113 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5464
Fragments arranged by size:
5464 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2047 2092 1186
Size: 861 45 4783
Fragments arranged by size:
4783 861 45
BseYI C'CCAG_C
Cuts at: 2396 4892 5388 2396
Size: 2496 496 2697
Fragments arranged by size:
2697 2496 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2534 1163
Size: 573 798 4318
Fragments arranged by size:
4318 798 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3139 3900 4928 1540
Size: 275 186 1138 761 1028 2301
Fragments arranged by size:
2301 1138 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3750 3835 4906 3750
Size: 85 1071 4533
Fragments arranged by size:
4533 1071 85
BsmI GAATG_Cn'
Cuts at: 3200 3293 3200
Size: 93 5596
BsmBI CGTCTCn'nnnn_
Cuts at: 2129 2129
Size: 5689
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3008 3750 3835 4906 1391
Size: 399 1218 742 85 1071 2174
Fragments arranged by size:
2174 1218 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5352 5352
Size: 5689
BspHI T'CATG_A
Cuts at: 3578 5308 3578
Size: 1730 3959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3044 844
Size: 2200 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3576 1394
Size: 429 1753 3507
Fragments arranged by size:
3507 1753 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4184 4358 65
Size: 1183 2936 174 1396
Fragments arranged by size:
2936 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2009 2137 2171 2448 4333 4520 1700
Size: 110 199 128 34 277 1885 187 2869
Fragments arranged by size:
2869 1885 277 199 187 128 110 34
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5689
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 2833 3749 4761 2833
Size: 916 1012 3761
Fragments arranged by size:
3761 1012 916
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2849 794
Size: 2055 3634
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5689
BstYI r'GATC_y
Cuts at: 1972 2374 2788 3019 3386 3771 3788 5240 5326
Size: 402 414 231 367 385 17 1452 86
Cuts at: 5326 5338 5684 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 414 402 385 367 346 231
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2640 2640
Size: 5689
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2824 487
Size: 683 56 1598 3352
Fragments arranged by size:
3352 1598 683 56
BtsI GCAGTG_nn'
Cuts at: 3205 3999 4019 3205
Size: 794 20 4875
Fragments arranged by size:
4875 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5654
DraI TTT'AAA
Cuts at: 2993 2993
Size: 5689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4696 809
Size: 507 3380 1802
Fragments arranged by size:
3380 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2139 3136 4025
Size: 54 1425 50 275 18 309 997 889
Cuts at: 4025 5654 8
Size: 1629 43
Fragments arranged by size:
1629 1425 997 889 309 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3136 1537
Size: 275 1324 4090
Fragments arranged by size:
4090 1324 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3619 1353
Size: 2266 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4276 4650 4796 387
Size: 1064 138 2687 374 146 1280
Fragments arranged by size:
2687 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2236 2463 2839 2946 3752 4349 5136
Size: 393 604 227 376 107 806 597 787
Cuts at: 5136 5404 1239
Size: 268 1524
Fragments arranged by size:
1524 806 787 604 597 393 376 268
227 107
EcoO109I rG'GnC_Cy
Cuts at: 2283 2400 2283
Size: 117 5572
EcoRI G'AATT_C
Cuts at: 2998 3376 2998
Size: 378 5311
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2291 2680 2291
Size: 389 5300
FspI TGC'GCA
Cuts at: 4195 4195
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 2178 4836 1678
Size: 500 2658 2531
Fragments arranged by size:
2658 2531 500
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2071 2392 2424 4330
Size: 32 135 32 467 32 321 32 1906
Cuts at: 4330 4362 4404 4436 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1906 467 321 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3033 3279 669
Size: 988 281 1095 246 3079
Fragments arranged by size:
3079 1095 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5689
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KasI G'GCGC_C
Cuts at: 2174 2174
Size: 5689
KpnI G_GTAC'C
Cuts at: 2830 3014 2830
Size: 184 5505
MfeI C'AATT_G
Cuts at: 2237 3288 2237
Size: 1051 4638
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2228 4803 4987 1305
Size: 923 2575 184 2007
Fragments arranged by size:
2575 2007 923 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3867
Fragments arranged by size:
3867 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2117 3647 4006 4165 518
Size: 648 951 1530 359 159 2042
Fragments arranged by size:
2042 1530 951 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3105 3782 4930 5175 1606
Size: 1499 677 1148 245 2120
Fragments arranged by size:
2120 1499 1148 677 245
NaeI GCC'GGC
Cuts at: 2139 2139
Size: 5689
NarI GG'CG_CC
Cuts at: 2175 2175
Size: 5689
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5137
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NgoMIV G'CCGG_C
Cuts at: 2137 2137
Size: 5689
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NsiI A_TGCA'T
Cuts at: 2250 2250
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 2213 3047 4592 1132
Size: 97 984 834 1545 2229
Fragments arranged by size:
2229 1545 984 834 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5689
PciI A'CATG_T
Cuts at: 4588 4588
Size: 5689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5689
PfoI T'CCnGG_A
Cuts at: 2181 2181
Size: 5689
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 5271 5303 3758
Size: 32 1481 32 4144
Fragments arranged by size:
4144 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2283 2400 2283
Size: 117 5572
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5689
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2492 2524 2492
Size: 32 5657
PstI C_TGCA'G
Cuts at: 830 3041 830
Size: 2211 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
SalI G'TCGA_C
Cuts at: 1936 3031 1936
Size: 1095 4594
SanDI GG'GwC_CC
Cuts at: 2283 2283
Size: 5689
SbfI CC_TGCA'GG
Cuts at: 3041 3041
Size: 5689
ScaI AGT'ACT
Cuts at: 3937 5649 3937
Size: 1712 3977
SfcI C'TryA_G
Cuts at: 826 1045 3037 4172 4853 5044 826
Size: 219 1992 1135 681 191 1471
Fragments arranged by size:
1992 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2176 2176
Size: 5689
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 2171 1700
Size: 471 5218
SmaI CCC'GGG
Cuts at: 1872 3016 1872
Size: 1144 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2156 3793 4694 4956 820
Size: 197 926 213 1637 901 262 1553
Fragments arranged by size:
1637 1553 926 901 262 213 197
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 2213 3047 1132
Size: 1081 834 3774
Fragments arranged by size:
3774 1081 834
SspI AAT'ATT
Cuts at: 5 52 2695 2782 3613 5
Size: 47 2643 87 831 2081
Fragments arranged by size:
2643 2081 831 87 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5689
StyI C'CwwG_G
Cuts at: 513 1065 3128 513
Size: 552 2063 3074
Fragments arranged by size:
3074 2063 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3716 3733 3886 4071 4516 325
Size: 1255 2136 17 153 185 445 1498
Fragments arranged by size:
2136 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2984 3935 5647
Size: 274 80 33 51 1443 1007 951 1712
Cuts at: 5647 96
Size: 138
Fragments arranged by size:
1712 1443 1007 951 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2905 2937 5649 5681 2905
Size: 32 2712 32 2913
Fragments arranged by size:
2913 2712 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5689
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2211 2211
Size: 5689
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5689
XmaI C'CCGG_G
Cuts at: 1870 3014 1870
Size: 1144 4545
XmnI GAAnn'nnTTC
Cuts at: 3818 3818
Size: 5689
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FseI FspAI NruI PacI PmlI PspOMI PspXI
PvuII RsrII SacI SacII SapI SexAI SfiI SrfI
SwaI