Restriction map for
pFN21AE1548
(Circular) (Six-base) MAPSORT of: pFN21AE1548.seq Check: 3981 from: 1 to: 5488
pFN21AE1548 5488 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2624 2624
Size: 5488
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2610 3298 278
Size: 53 83 186 936 1074 688 2468
Fragments arranged by size:
2468 1074 936 688 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2610 2831 1937
Size: 673 221 4594
Fragments arranged by size:
4594 673 221
Acc65I G'GTAC_C
Cuts at: 2809 2809
Size: 5488
AclI AA'CG_TT
Cuts at: 1563 3615 3988 1563
Size: 2052 373 3063
Fragments arranged by size:
3063 2052 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3551 4935 5203 1632
Size: 1919 1384 268 1917
Fragments arranged by size:
1919 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5291
AflIII A'CryG_T
Cuts at: 1203 4387 5364 1203
Size: 3184 977 1327
Fragments arranged by size:
3184 1327 977
AgeI A'CCGG_T
Cuts at: 4319 4319
Size: 5488
AhdI GACnn_n'nnGTC
Cuts at: 4217 4217
Size: 5488
AleI CACnn'nnGTG
Cuts at: 1166 2087 1166
Size: 921 4567
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2387 1184
Size: 1203 4285
AlwNI CAG_nnn'CTG
Cuts at: 2572 3231 4803 2572
Size: 659 1572 3257
Fragments arranged by size:
3257 1572 659
ApaLI G'TGCA_C
Cuts at: 3545 4701 3545
Size: 1156 4332
ApoI r'AATT_y
Cuts at: 1493 2797 3023 3175 5284 1493
Size: 1304 226 152 2109 1697
Fragments arranged by size:
2109 1697 1304 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4042 160
Size: 872 3010 1606
Fragments arranged by size:
3010 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
AvaI C'yCGr_G
Cuts at: 1870 1943 2111 2313 2738 2813 1870
Size: 73 168 202 425 75 4545
Fragments arranged by size:
4545 425 202 168 75 73
BamHI G'GATC_C
Cuts at: 2028 2818 3185 2028
Size: 790 367 4331
Fragments arranged by size:
4331 790 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2809 4264 618
Size: 325 217 627 1022 1455 1842
Fragments arranged by size:
1842 1455 1022 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2361 2391 2671 2781 2807 1391
Size: 970 30 280 110 26 4072
Fragments arranged by size:
4072 970 280 110 30 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4517
BbvCI CC'TCA_GC
Cuts at: 2094 2094
Size: 5488
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3679 3713 1903
Size: 34 1742 34 3678
Fragments arranged by size:
3678 1742 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3380 4590 3380
Size: 1210 4278
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5488
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2260 4099 136
Size: 107 122 71 631 605 588 1839 1525
Fragments arranged by size:
1839 1525 631 605 588 122 107 71
BglII A'GATC_T
Cuts at: 5483 5483
Size: 5488
BlpI GC'TnA_GC
Cuts at: 2078 2905 2078
Size: 827 4661
Bme1580I G_kGCm'C
Cuts at: 1790 3549 4705 1790
Size: 1759 1156 2573
Fragments arranged by size:
2573 1759 1156
BmgBI CAC'GTC
Cuts at: 1318 2230 1318
Size: 912 4576
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3219 4177 442
Size: 416 53 83 399 24 1802 958 1753
Fragments arranged by size:
1802 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2055 2324 2624 4148 1239
Size: 816 269 300 1524 2579
Fragments arranged by size:
2579 1524 816 300 269
Bpu10I CC'TnA_GC
Cuts at: 2094 2094
Size: 5488
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3613 4478 4776 3613
Size: 865 298 4325
Fragments arranged by size:
4325 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4151 882
Size: 617 2652 2219
Fragments arranged by size:
2652 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2623 3279 493
Size: 711 1419 656 2702
Fragments arranged by size:
2702 1419 711 656
BsaBI GATnn'nnATC
Cuts at: 2466 5482 2466
Size: 3016 2472
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2252 2607 3295 3677
Size: 53 83 186 936 719 355 688 382
Cuts at: 3677 4312 275
Size: 635 1451
Fragments arranged by size:
1451 936 719 688 635 382 355 186
83 53
BsaWI w'CCGG_w
Cuts at: 2487 3921 4319 4593 4740 5151 2487
Size: 1434 398 274 147 411 2824
Fragments arranged by size:
2824 1434 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2703 2733 1178
Size: 30 165 30 1300 30 3933
Fragments arranged by size:
3933 1300 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2219 2525 1186
Size: 1033 306 4149
Fragments arranged by size:
4149 1033 306
BseYI C'CCAG_C
Cuts at: 2257 2595 2633 2722 2743 4691 5187 2257
Size: 338 38 89 21 1948 496 2558
Fragments arranged by size:
2558 1948 496 338 89 38 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2356 2768 1163
Size: 573 620 412 3883
Fragments arranged by size:
3883 620 573 412
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2938 3699 4727 1540
Size: 275 186 937 761 1028 2301
Fragments arranged by size:
2301 1028 937 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2084 2361 2631 3549 3634 4705 2084
Size: 277 270 918 85 1071 2867
Fragments arranged by size:
2867 1071 918 277 270 85
BsmI GAATG_Cn'
Cuts at: 2999 3092 2999
Size: 93 5395
BsmBI CGTCTCn'nnnn_
Cuts at: 2598 2598
Size: 5488
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2084 2361 2391 2631 2671 2781 2807
Size: 399 294 277 30 240 40 110 26
Cuts at: 2807 3549 3634 4705 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 294 277 240 110
85 40 30 26
BspEI T'CCGG_A
Cuts at: 5151 5151
Size: 5488
BspHI T'CATG_A
Cuts at: 3377 5107 3377
Size: 1730 3758
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2448 2624 2843 844
Size: 1604 176 219 3489
Fragments arranged by size:
3489 1604 219 176
BsrBI CCG'CTC
Cuts at: 1394 1823 3375 1394
Size: 429 1552 3507
Fragments arranged by size:
3507 1552 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3983 4157 65
Size: 1183 2735 174 1396
Fragments arranged by size:
2735 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4132 4319 1700
Size: 110 2322 187 2869
Fragments arranged by size:
2869 2322 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5488
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5488
BssSI C'ACGA_G
Cuts at: 3548 4560 3548
Size: 1012 4476
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5488
BstBI TT'CG_AA
Cuts at: 4292 4292
Size: 5488
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5488
BstXI CCAn_nnnn'nTGG
Cuts at: 2656 2656
Size: 5488
BstYI r'GATC_y
Cuts at: 1972 2028 2568 2818 3185 3570 3587 5039 5125
Size: 56 540 250 367 385 17 1452 86
Cuts at: 5125 5137 5483 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 540 385 367 346 250 86
56 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4936
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2087 487
Size: 683 56 861 3888
Fragments arranged by size:
3888 861 683 56
BtsI GCAGTG_nn'
Cuts at: 3004 3798 3818 3004
Size: 794 20 4674
Fragments arranged by size:
4674 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5488
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5453
DraI TTT'AAA
Cuts at: 2792 2792
Size: 5488
DraIII CAC_nnn'GTG
Cuts at: 2334 2334
Size: 5488
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4495 809
Size: 507 3179 1802
Fragments arranged by size:
3179 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2935 3824 5453
Size: 54 1425 50 275 18 1105 889 1629
Cuts at: 5453 8
Size: 43
Fragments arranged by size:
1629 1425 1105 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2935 1537
Size: 275 1123 4090
Fragments arranged by size:
4090 1123 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3418 1353
Size: 2065 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2333 4075 4449 4595 387
Size: 1064 138 744 1742 374 146 1280
Fragments arranged by size:
1742 1280 1064 744 374 146 138
EcoICRI GAG'CTC
Cuts at: 2359 2359
Size: 5488
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2055 2324 2624 3551 4148 4935 5203
Size: 393 423 269 300 927 597 787 268
Cuts at: 5203 1239
Size: 1524
Fragments arranged by size:
1524 927 787 597 423 393 300 269
268
EcoO109I rG'GnC_Cy
Cuts at: 2310 2422 2310
Size: 112 5376
EcoRI G'AATT_C
Cuts at: 2797 3175 2797
Size: 378 5110
FspI TGC'GCA
Cuts at: 3994 3994
Size: 5488
HaeII r_GCGC'y
Cuts at: 1678 4635 1678
Size: 2957 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4129 4161 4203 4235 1373
Size: 32 135 32 2557 32 42 32 2626
Fragments arranged by size:
2626 2557 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2832 3078 669
Size: 988 281 894 246 3079
Fragments arranged by size:
3079 988 894 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5488
HpaI GTT'AAC
Cuts at: 3078 3078
Size: 5488
KpnI G_GTAC'C
Cuts at: 2813 2813
Size: 5488
MfeI C'AATT_G
Cuts at: 3087 3087
Size: 5488
MluI A'CGCG_T
Cuts at: 5364 5364
Size: 5488
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2304 2334 4602 4786 1305
Size: 999 30 2268 184 2007
Fragments arranged by size:
2268 2007 999 184 30
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3666
Fragments arranged by size:
3666 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2087 2654 3446 3805 3964 518
Size: 648 921 567 792 359 159 2042
Fragments arranged by size:
2042 921 792 648 567 359 159
MspA1I CmG'CkG
Cuts at: 1606 2257 2377 2904 3581 4729 4974 1606
Size: 651 120 527 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 651 527 245 120
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4936
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5488
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5488
NotI GC'GGCC_GC
Cuts at: 2935 2935
Size: 5488
NspI r_CATG'y
Cuts at: 1132 1229 2143 2846 4391 1132
Size: 97 914 703 1545 2229
Fragments arranged by size:
2229 1545 914 703 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5488
PciI A'CATG_T
Cuts at: 4387 4387
Size: 5488
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2572 1108
Size: 902 562 4024
Fragments arranged by size:
4024 902 562
PfoI T'CCnGG_A
Cuts at: 2301 2411 2301
Size: 110 5378
PmeI GTTT'AAAC
Cuts at: 2792 2792
Size: 5488
PmlI CAC'GTG
Cuts at: 2623 2623
Size: 5488
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3557 3589 5070 5102 3557
Size: 32 1481 32 3943
Fragments arranged by size:
3943 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2310 2310
Size: 5488
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5488
PsiI TTA'TAA
Cuts at: 3058 3058
Size: 5488
PstI C_TGCA'G
Cuts at: 830 2840 830
Size: 2010 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5488
SacI G_AGCT'C
Cuts at: 2361 2361
Size: 5488
SalI G'TCGA_C
Cuts at: 1936 2830 1936
Size: 894 4594
SbfI CC_TGCA'GG
Cuts at: 2840 2840
Size: 5488
ScaI AGT'ACT
Cuts at: 2279 3736 5448 2279
Size: 1457 1712 2319
Fragments arranged by size:
2319 1712 1457
SfcI C'TryA_G
Cuts at: 826 1045 2836 3971 4652 4843 826
Size: 219 1791 1135 681 191 1471
Fragments arranged by size:
1791 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5488
SmaI CCC'GGG
Cuts at: 1872 2315 2815 1872
Size: 443 500 4545
Fragments arranged by size:
4545 500 443
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3592 4493 4755 820
Size: 197 926 1649 901 262 1553
Fragments arranged by size:
1649 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3279 493
Size: 2786 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5488
SphI G_CATG'C
Cuts at: 1132 2846 1132
Size: 1714 3774
SspI AAT'ATT
Cuts at: 5 52 3412 5
Size: 47 3360 2081
Fragments arranged by size:
3360 2081 47
StuI AGG'CCT
Cuts at: 1517 2040 1517
Size: 523 4965
StyI C'CwwG_G
Cuts at: 513 1065 2927 513
Size: 552 1862 3074
Fragments arranged by size:
3074 1862 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3515 3532 3685 3870 4315 325
Size: 1255 1935 17 153 185 445 1498
Fragments arranged by size:
1935 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2277 3734 5446
Size: 274 80 33 51 1443 300 1457 1712
Cuts at: 5446 96
Size: 138
Fragments arranged by size:
1712 1457 1443 300 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2318 2350 2703 2735 5448 5480 2318
Size: 32 353 32 2713 32 2326
Fragments arranged by size:
2713 2326 353 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5488
XbaI T'CTAG_A
Cuts at: 2824 2824
Size: 5488
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5488
XmaI C'CCGG_G
Cuts at: 1870 2313 2813 1870
Size: 443 500 4545
Fragments arranged by size:
4545 500 443
XmnI GAAnn'nnTTC
Cuts at: 3617 3617
Size: 5488
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2608 3296 276
Size: 53 83 186 936 1074 688 2468
Fragments arranged by size:
2468 1074 936 688 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI