Restriction map for
pFN21AE1547
(Circular) (Six-base) MAPSORT of: pFN21AE1547.seq Check: 4890 from: 1 to: 6478
pFN21AE1547 6478 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4288 278
Size: 53 83 186 936 2752 2468
Fragments arranged by size:
2752 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3821 1937
Size: 1884 4594
Acc65I G'GTAC_C
Cuts at: 3799 3799
Size: 6478
AclI AA'CG_TT
Cuts at: 1563 4605 4978 1563
Size: 3042 373 3063
Fragments arranged by size:
3063 3042 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2663 4541 5925 6193 1632
Size: 1031 1878 1384 268 1917
Fragments arranged by size:
1917 1878 1384 1031 268
AflII C'TTAA_G
Cuts at: 820 1017 3221 820
Size: 197 2204 4077
Fragments arranged by size:
4077 2204 197
AflIII A'CryG_T
Cuts at: 1203 3126 5377 6354 1203
Size: 1923 2251 977 1327
Fragments arranged by size:
2251 1923 1327 977
AgeI A'CCGG_T
Cuts at: 5309 5309
Size: 6478
AhdI GACnn_n'nnGTC
Cuts at: 5207 5207
Size: 6478
AleI CACnn'nnGTG
Cuts at: 1166 3589 3683 1166
Size: 2423 94 3961
Fragments arranged by size:
3961 2423 94
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6478
AlwNI CAG_nnn'CTG
Cuts at: 4221 5793 4221
Size: 1572 4906
ApaI G_GGCC'C
Cuts at: 3262 3262
Size: 6478
ApaLI G'TGCA_C
Cuts at: 3582 4535 5691 3582
Size: 953 1156 4369
Fragments arranged by size:
4369 1156 953
ApoI r'AATT_y
Cuts at: 1493 3787 4013 4165 6274 1493
Size: 2294 226 152 2109 1697
Fragments arranged by size:
2294 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5032 160
Size: 872 4000 1606
Fragments arranged by size:
4000 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6478
AvaI C'yCGr_G
Cuts at: 1870 1943 2115 2478 3200 3612 3803 1870
Size: 73 172 363 722 412 191 4545
Fragments arranged by size:
4545 722 412 363 191 172 73
BamHI G'GATC_C
Cuts at: 3808 4175 3808
Size: 367 6111
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 3083 3685 3799 5254
Size: 325 217 627 248 1048 602 114 1455
Cuts at: 5254 618
Size: 1842
Fragments arranged by size:
1842 1455 1048 627 602 325 248 217
114
BanII G_rGCy'C
Cuts at: 1391 2460 2774 3058 3262 3797 1391
Size: 1069 314 284 204 535 4072
Fragments arranged by size:
4072 1069 535 314 284 204
BbeI G_GCGC'C
Cuts at: 2039 2039
Size: 6478
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3756 928
Size: 971 1857 3650
Fragments arranged by size:
3650 1857 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3671 3705 4669 4703 1903
Size: 34 1734 34 964 34 3678
Fragments arranged by size:
3678 1734 964 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4370 5580 4370
Size: 1210 5268
BclI T'GATC_A
Cuts at: 1548 2622 1548
Size: 1074 5404
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5089 136
Size: 107 122 71 631 605 3417 1525
Fragments arranged by size:
3417 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6473 6473
Size: 6478
BlpI GC'TnA_GC
Cuts at: 2459 3895 2459
Size: 1436 5042
Bme1580I G_kGCm'C
Cuts at: 1790 3262 3586 4539 5695 1790
Size: 1472 324 953 1156 2573
Fragments arranged by size:
2573 1472 1156 953 324
BmgBI CAC'GTC
Cuts at: 1318 3129 1318
Size: 1811 4667
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3442 3532 4209
Size: 416 53 83 399 24 2025 90 677
Cuts at: 4209 5167 442
Size: 958 1753
Fragments arranged by size:
2025 1753 958 677 416 399 90 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6478
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2474 2474
Size: 6478
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2382 5138 1239
Size: 1143 2756 2579
Fragments arranged by size:
2756 2579 1143
Bpu10I CC'TnA_GC
Cuts at: 2398 2852 2398
Size: 454 6024
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4603 5468 5766 4603
Size: 865 298 5315
Fragments arranged by size:
5315 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5141 882
Size: 617 3642 2219
Fragments arranged by size:
3642 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4269 493
Size: 711 3065 2702
Fragments arranged by size:
3065 2702 711
BsaBI GATnn'nnATC
Cuts at: 2970 6472 2970
Size: 3502 2976
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2036 4285 4667 5302
Size: 53 83 186 936 503 2249 382 635
Cuts at: 5302 275
Size: 1451
Fragments arranged by size:
2249 1451 936 635 503 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4911 5309 5583 5730 6141 4911
Size: 398 274 147 411 5248
Fragments arranged by size:
5248 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2879 2909 1178
Size: 30 165 30 1476 30 4747
Fragments arranged by size:
4747 1476 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2735 1186
Size: 1549 4929
BseYI C'CCAG_C
Cuts at: 5681 6177 5681
Size: 496 5982
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5905
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3928 4689 5717 1540
Size: 275 186 1927 761 1028 2301
Fragments arranged by size:
2301 1927 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2460 2774 3586 4539 4624 5695 2460
Size: 314 812 953 85 1071 3243
Fragments arranged by size:
3243 1071 953 812 314 85
BsmI GAATG_Cn'
Cuts at: 3989 4082 3989
Size: 93 6385
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2460 2774 3058 3262 3586 3797 4539
Size: 399 670 314 284 204 324 211 742
Cuts at: 4539 4624 5695 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 670 399 324 314 284
211 204 85
BspEI T'CCGG_A
Cuts at: 6141 6141
Size: 6478
BspHI T'CATG_A
Cuts at: 3415 4367 6097 3415
Size: 952 1730 3796
Fragments arranged by size:
3796 1730 952
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3715 3833 844
Size: 2871 118 3489
Fragments arranged by size:
3489 2871 118
BsrBI CCG'CTC
Cuts at: 1394 1823 2122 4365 1394
Size: 429 299 2243 3507
Fragments arranged by size:
3507 2243 429 299
BsrDI GCAATG_nn'
Cuts at: 65 1248 2177 4973 5147 65
Size: 1183 929 2796 174 1396
Fragments arranged by size:
2796 1396 1183 929 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5122 5309 1700
Size: 110 3312 187 2869
Fragments arranged by size:
3312 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6478
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6478
BssSI C'ACGA_G
Cuts at: 4538 5550 4538
Size: 1012 5466
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2095 794
Size: 1301 5177
BstBI TT'CG_AA
Cuts at: 2524 5282 2524
Size: 2758 3720
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6478
BstXI CCAn_nnnn'nTGG
Cuts at: 2667 2667
Size: 6478
BstYI r'GATC_y
Cuts at: 1972 3808 4175 4560 4577 6029 6115 6127 6473
Size: 1836 367 385 17 1452 86 12 346
Cuts at: 6473 1972
Size: 1977
Fragments arranged by size:
1977 1836 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3369 513
Size: 552 939 1365 3622
Fragments arranged by size:
3622 1365 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5739
Fragments arranged by size:
5739 683 56
BtsI GCAGTG_nn'
Cuts at: 2037 3516 3994 4788 4808 2037
Size: 1479 478 794 20 3707
Fragments arranged by size:
3707 1479 794 478 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6478
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3741 3776 529
Size: 35 3177 35 3231
Fragments arranged by size:
3231 3177 35 35
DraI TTT'AAA
Cuts at: 3782 3782
Size: 6478
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5485 809
Size: 507 4169 1802
Fragments arranged by size:
4169 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2187 2253 3925
Size: 54 1425 50 275 18 357 66 1672
Cuts at: 3925 4814 6443 8
Size: 889 1629 43
Fragments arranged by size:
1672 1629 1425 889 357 275 66 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3925 1537
Size: 275 2113 4090
Fragments arranged by size:
4090 2113 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4408 1353
Size: 3055 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2168 2196 5065 5439 5585 387
Size: 1064 138 579 28 2869 374 146 1280
Fragments arranged by size:
2869 1280 1064 579 374 146 138 28
EcoICRI GAG'CTC
Cuts at: 2458 2772 2458
Size: 314 6164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2382 2663 4541 5138 5925 6193 1239
Size: 393 750 281 1878 597 787 268 1524
Fragments arranged by size:
1878 1524 787 750 597 393 281 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2255 2255
Size: 6478
EcoO109I rG'GnC_Cy
Cuts at: 2125 2402 2725 2125
Size: 277 323 5878
Fragments arranged by size:
5878 323 277
EcoRI G'AATT_C
Cuts at: 3787 4165 3787
Size: 378 6100
EcoRV GAT'ATC
Cuts at: 2354 3475 2354
Size: 1121 5357
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2281 2281
Size: 6478
FspI TGC'GCA
Cuts at: 4984 4984
Size: 6478
HaeII r_GCGC'y
Cuts at: 1678 2039 2438 5625 1678
Size: 361 399 3187 2531
Fragments arranged by size:
3187 2531 399 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2879 2911 5119 5151 5193
Size: 32 135 32 1307 32 2208 32 42
Cuts at: 5193 5225 1373
Size: 32 2626
Fragments arranged by size:
2626 2208 1307 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3169 3426 3822 4068 669
Size: 988 281 1231 257 396 246 3079
Fragments arranged by size:
3079 1231 988 396 281 257 246
HindIII A'AGCT_T
Cuts at: 748 3410 3570 748
Size: 2662 160 3656
Fragments arranged by size:
3656 2662 160
HpaI GTT'AAC
Cuts at: 4068 4068
Size: 6478
KasI G'GCGC_C
Cuts at: 2035 2035
Size: 6478
KpnI G_GTAC'C
Cuts at: 3803 3803
Size: 6478
MfeI C'AATT_G
Cuts at: 4077 4077
Size: 6478
MluI A'CGCG_T
Cuts at: 6354 6354
Size: 6478
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2922 5592 5776 1305
Size: 1617 2670 184 2007
Fragments arranged by size:
2670 2007 1617 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2255 10
Size: 54 1425 343 423 4233
Fragments arranged by size:
4233 1425 423 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2555 2669 2753 3116 3589 3683 4436
Size: 648 1389 114 84 363 473 94 753
Cuts at: 4436 4795 4954 518
Size: 359 159 2042
Fragments arranged by size:
2042 1389 753 648 473 363 359 159
114 94 84
MspA1I CmG'CkG
Cuts at: 1606 3894 4571 5719 5964 1606
Size: 2288 677 1148 245 2120
Fragments arranged by size:
2288 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 2036 2036
Size: 6478
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4987
Fragments arranged by size:
4987 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6478
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6478
NotI GC'GGCC_GC
Cuts at: 3925 3925
Size: 6478
NspI r_CATG'y
Cuts at: 1132 1229 3158 3292 3836 5381 1132
Size: 97 1929 134 544 1545 2229
Fragments arranged by size:
2229 1929 1545 544 134 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6478
PciI A'CATG_T
Cuts at: 5377 5377
Size: 6478
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2236 1108
Size: 1128 5350
PmeI GTTT'AAAC
Cuts at: 3782 3782
Size: 6478
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4547 4579 6060 6092 4547
Size: 32 1481 32 4933
Fragments arranged by size:
4933 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6478
PsiI TTA'TAA
Cuts at: 4048 4048
Size: 6478
PspOMI G'GGCC_C
Cuts at: 3258 3258
Size: 6478
PstI C_TGCA'G
Cuts at: 830 3830 830
Size: 3000 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6478
SacI G_AGCT'C
Cuts at: 2460 2774 2460
Size: 314 6164
SalI G'TCGA_C
Cuts at: 1936 3820 1936
Size: 1884 4594
SbfI CC_TGCA'GG
Cuts at: 3830 3830
Size: 6478
ScaI AGT'ACT
Cuts at: 3542 4726 6438 3542
Size: 1184 1712 3582
Fragments arranged by size:
3582 1712 1184
SfcI C'TryA_G
Cuts at: 826 1045 3181 3647 3826 4961 5642 5833 826
Size: 219 2136 466 179 1135 681 191 1471
Fragments arranged by size:
2136 1471 1135 681 466 219 191 179
SfoI GGC'GCC
Cuts at: 2037 2037
Size: 6478
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6478
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6478
SmaI CCC'GGG
Cuts at: 1872 2117 3614 3805 1872
Size: 245 1497 191 4545
Fragments arranged by size:
4545 1497 245 191
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2478 3221 4582 5483 5745 820
Size: 197 926 535 743 1361 901 262 1553
Fragments arranged by size:
1553 1361 926 901 743 535 262 197
SnaBI TAC'GTA
Cuts at: 493 4269 493
Size: 3776 2702
SpeI A'CTAG_T
Cuts at: 152 3331 152
Size: 3179 3299
SphI G_CATG'C
Cuts at: 1132 3292 3836 1132
Size: 2160 544 3774
Fragments arranged by size:
3774 2160 544
SspI AAT'ATT
Cuts at: 5 52 4402 5
Size: 47 4350 2081
Fragments arranged by size:
4350 2081 47
StuI AGG'CCT
Cuts at: 1517 2261 3629 1517
Size: 744 1368 4366
Fragments arranged by size:
4366 1368 744
StyI C'CwwG_G
Cuts at: 513 1065 2004 2256 3661 3917 513
Size: 552 939 252 1405 256 3074
Fragments arranged by size:
3074 1405 939 552 256 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4505 4522 4675 4860 5305 325
Size: 1255 2925 17 153 185 445 1498
Fragments arranged by size:
2925 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3108 3540 4724
Size: 274 80 33 51 1443 1131 432 1184
Cuts at: 4724 6436 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1184 1131 432 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6438 6470 6438
Size: 32 6446
Tth111I GACn'n_nGTC
Cuts at: 1316 3430 1316
Size: 2114 4364
XbaI T'CTAG_A
Cuts at: 3814 3814
Size: 6478
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6478
XhoI C'TCGA_G
Cuts at: 1943 2478 1943
Size: 535 5943
XmaI C'CCGG_G
Cuts at: 1870 2115 3612 3803 1870
Size: 245 1497 191 4545
Fragments arranged by size:
4545 1497 245 191
XmnI GAAnn'nnTTC
Cuts at: 4607 4607
Size: 6478
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4286 276
Size: 53 83 186 936 2752 2468
Fragments arranged by size:
2752 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SrfI SwaI