Restriction map for
pFN21AE1545
(Circular) (Six-base) MAPSORT of: pFN21AE1545.seq Check: 8190 from: 1 to: 5926
pFN21AE1545 5926 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3736 278
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3269 1937
Size: 1332 4594
Acc65I G'GTAC_C
Cuts at: 3247 3247
Size: 5926
AclI AA'CG_TT
Cuts at: 1563 4053 4426 1563
Size: 2490 373 3063
Fragments arranged by size:
3063 2490 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2475 3159 3989 5373 5641 1632
Size: 843 684 830 1384 268 1917
Fragments arranged by size:
1917 1384 843 830 684 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5729
AflIII A'CryG_T
Cuts at: 1203 4825 5802 1203
Size: 3622 977 1327
Fragments arranged by size:
3622 1327 977
AgeI A'CCGG_T
Cuts at: 4757 4757
Size: 5926
AhdI GACnn_n'nnGTC
Cuts at: 4655 4655
Size: 5926
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5926
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5926
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2282 2314 2409 2441 2282
Size: 32 95 32 5767
Fragments arranged by size:
5767 95 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2246 2906 3669 5241 2246
Size: 660 763 1572 2931
Fragments arranged by size:
2931 1572 763 660
ApaLI G'TGCA_C
Cuts at: 3983 5139 3983
Size: 1156 4770
ApoI r'AATT_y
Cuts at: 1493 2119 2684 2706 2957 3235 3461 3613 5722
Size: 626 565 22 251 278 226 152 2109
Cuts at: 5722 1493
Size: 1697
Fragments arranged by size:
2109 1697 626 565 278 251 226 152
22
AseI AT'TA_AT
Cuts at: 160 1032 2760 4480 160
Size: 872 1728 1720 1606
Fragments arranged by size:
1728 1720 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
AvaI C'yCGr_G
Cuts at: 1870 1943 2483 3251 1870
Size: 73 540 768 4545
Fragments arranged by size:
4545 768 540 73
BamHI G'GATC_C
Cuts at: 3256 3623 3256
Size: 367 5559
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3247 4702 618
Size: 325 217 627 1460 1455 1842
Fragments arranged by size:
1842 1460 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3245 1391
Size: 1854 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3150 928
Size: 971 1251 3704
Fragments arranged by size:
3704 1251 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4117 4151 1903
Size: 34 2180 34 3678
Fragments arranged by size:
3678 2180 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2782 3818 5028 2782
Size: 1036 1210 3680
Fragments arranged by size:
3680 1210 1036
BclI T'GATC_A
Cuts at: 1548 2985 1548
Size: 1437 4489
BfrBI ATG'CAT
Cuts at: 2884 2884
Size: 5926
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4537 136
Size: 107 122 71 631 605 2865 1525
Fragments arranged by size:
2865 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5921 5921
Size: 5926
BlpI GC'TnA_GC
Cuts at: 3343 3343
Size: 5926
Bme1580I G_kGCm'C
Cuts at: 1790 3987 5143 1790
Size: 2197 1156 2573
Fragments arranged by size:
2573 2197 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5926
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3657 4615 442
Size: 416 53 83 399 24 2240 958 1753
Fragments arranged by size:
2240 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4586 1239
Size: 3347 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2214 4051 4916 5214 2214
Size: 1837 865 298 2926
Fragments arranged by size:
2926 1837 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2300 4589 882
Size: 617 801 2289 2219
Fragments arranged by size:
2289 2219 801 617
BsaAI yAC'GTr
Cuts at: 493 1204 3717 493
Size: 711 2513 2702
Fragments arranged by size:
2702 2513 711
BsaBI GATnn'nnATC
Cuts at: 5920 5920
Size: 5926
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3733 4115 4750 275
Size: 53 83 186 936 2200 382 635 1451
Fragments arranged by size:
2200 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2961 4359 4757 5031 5178 5589 2961
Size: 1398 398 274 147 411 3298
Fragments arranged by size:
3298 1398 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2282 2312 3100 3130 1178
Size: 30 165 30 879 30 788 30 3974
Fragments arranged by size:
3974 879 788 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3209 1186
Size: 2023 3903
BseYI C'CCAG_C
Cuts at: 5129 5625 5129
Size: 496 5430
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5353
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3376 4137 5165 1540
Size: 275 186 1375 761 1028 2301
Fragments arranged by size:
2301 1375 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3987 4072 5143 3987
Size: 85 1071 4770
Fragments arranged by size:
4770 1071 85
BsmI GAATG_Cn'
Cuts at: 3437 3530 3437
Size: 93 5833
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3245 3987 4072 5143 1391
Size: 399 1455 742 85 1071 2174
Fragments arranged by size:
2174 1455 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5589 5589
Size: 5926
BspHI T'CATG_A
Cuts at: 2688 2949 3815 5545 2688
Size: 261 866 1730 3069
Fragments arranged by size:
3069 1730 866 261
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3281 844
Size: 2437 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3813 1394
Size: 429 1990 3507
Fragments arranged by size:
3507 1990 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4421 4595 65
Size: 1183 3173 174 1396
Fragments arranged by size:
3173 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4570 4757 1700
Size: 110 2760 187 2869
Fragments arranged by size:
2869 2760 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5926
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5926
BssSI C'ACGA_G
Cuts at: 3986 4998 3986
Size: 1012 4914
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2446 794
Size: 1652 4274
BstBI TT'CG_AA
Cuts at: 2428 2704 4730 2428
Size: 276 2026 3624
Fragments arranged by size:
3624 2026 276
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5926
BstXI CCAn_nnnn'nTGG
Cuts at: 2341 3211 2341
Size: 870 5056
BstYI r'GATC_y
Cuts at: 1972 2352 2695 3256 3623 4008 4025 5477 5563
Size: 380 343 561 367 385 17 1452 86
Cuts at: 5563 5575 5921 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 561 385 380 367 346 343
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2019 2575 513
Size: 552 954 556 3864
Fragments arranged by size:
3864 954 556 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5187
Fragments arranged by size:
5187 683 56
BtsI GCAGTG_nn'
Cuts at: 3442 4236 4256 3442
Size: 794 20 5112
Fragments arranged by size:
5112 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5926
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5891
DraI TTT'AAA
Cuts at: 2124 3230 2124
Size: 1106 4820
DraIII CAC_nnn'GTG
Cuts at: 2798 2798
Size: 5926
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4933 809
Size: 507 3617 1802
Fragments arranged by size:
3617 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3373 4262 5891
Size: 54 1425 50 275 18 1543 889 1629
Cuts at: 5891 8
Size: 43
Fragments arranged by size:
1629 1543 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3373 1537
Size: 275 1561 4090
Fragments arranged by size:
4090 1561 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3856 1353
Size: 2503 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4513 4887 5033 387
Size: 1064 138 2924 374 146 1280
Fragments arranged by size:
2924 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2475 3159 3989 4586 5373 5641 1239
Size: 393 843 684 830 597 787 268 1524
Fragments arranged by size:
1524 843 830 787 684 597 393 268
EcoRI G'AATT_C
Cuts at: 2706 2957 3235 3613 2706
Size: 251 278 378 5019
Fragments arranged by size:
5019 378 278 251
FspI TGC'GCA
Cuts at: 4432 4432
Size: 5926
HaeII r_GCGC'y
Cuts at: 1678 5073 1678
Size: 3395 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2931 2963 4567 4599 4641
Size: 32 135 32 1359 32 1604 32 42
Cuts at: 4641 4673 1373
Size: 32 2626
Fragments arranged by size:
2626 1604 1359 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3270 3516 669
Size: 988 281 1332 246 3079
Fragments arranged by size:
3079 1332 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5926
HpaI GTT'AAC
Cuts at: 3516 3516
Size: 5926
KpnI G_GTAC'C
Cuts at: 3251 3251
Size: 5926
MfeI C'AATT_G
Cuts at: 3525 3525
Size: 5926
MluI A'CGCG_T
Cuts at: 5802 5802
Size: 5926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2268 2649 2766 5040 5224 1305
Size: 963 381 117 2274 184 2007
Fragments arranged by size:
2274 2007 963 381 184 117
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4104
Fragments arranged by size:
4104 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3209 3884 4243 4402 518
Size: 648 2043 675 359 159 2042
Fragments arranged by size:
2043 2042 675 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2021 3342 4019 5167 5412 1606
Size: 415 1321 677 1148 245 2120
Fragments arranged by size:
2120 1321 1148 677 415 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5374
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5926
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5926
NotI GC'GGCC_GC
Cuts at: 3373 3373
Size: 5926
NsiI A_TGCA'T
Cuts at: 2886 2886
Size: 5926
NspI r_CATG'y
Cuts at: 1132 1229 2888 3284 4829 1132
Size: 97 1659 396 1545 2229
Fragments arranged by size:
2229 1659 1545 396 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5926
PciI A'CATG_T
Cuts at: 4825 4825
Size: 5926
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5926
PmeI GTTT'AAAC
Cuts at: 3230 3230
Size: 5926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2282 2314 3995 4027 5508 5540 2282
Size: 32 1681 32 1481 32 2668
Fragments arranged by size:
2668 1681 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5926
PsiI TTA'TAA
Cuts at: 2028 3041 3496 2028
Size: 1013 455 4458
Fragments arranged by size:
4458 1013 455
PstI C_TGCA'G
Cuts at: 830 2451 3278 830
Size: 1621 827 3478
Fragments arranged by size:
3478 1621 827
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5926
SacII CC_GC'GG
Cuts at: 2022 2022
Size: 5926
SalI G'TCGA_C
Cuts at: 1936 3268 1936
Size: 1332 4594
SbfI CC_TGCA'GG
Cuts at: 3278 3278
Size: 5926
ScaI AGT'ACT
Cuts at: 4174 5886 4174
Size: 1712 4214
SfcI C'TryA_G
Cuts at: 826 1045 2447 3186 3274 4409 5090 5281 826
Size: 219 1402 739 88 1135 681 191 1471
Fragments arranged by size:
1471 1402 1135 739 681 219 191 88
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5926
SmaI CCC'GGG
Cuts at: 1872 2485 3253 1872
Size: 613 768 4545
Fragments arranged by size:
4545 768 613
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2229 4030 4931 5193 820
Size: 197 926 286 1801 901 262 1553
Fragments arranged by size:
1801 1553 926 901 286 262 197
SnaBI TAC'GTA
Cuts at: 493 3717 493
Size: 3224 2702
SpeI A'CTAG_T
Cuts at: 152 2317 152
Size: 2165 3761
SphI G_CATG'C
Cuts at: 1132 2888 3284 1132
Size: 1756 396 3774
Fragments arranged by size:
3774 1756 396
SspI AAT'ATT
Cuts at: 5 52 3850 5
Size: 47 3798 2081
Fragments arranged by size:
3798 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5926
StyI C'CwwG_G
Cuts at: 513 1065 3365 513
Size: 552 2300 3074
Fragments arranged by size:
3074 2300 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3953 3970 4123 4308 4753 325
Size: 1255 2373 17 153 185 445 1498
Fragments arranged by size:
2373 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4172 5884 96
Size: 274 80 33 51 1443 2195 1712 138
Fragments arranged by size:
2195 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3100 3132 3145 3177 5886 5918 3100
Size: 32 13 32 2709 32 3108
Fragments arranged by size:
3108 2709 32 32 32 13
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5926
XbaI T'CTAG_A
Cuts at: 3262 3262
Size: 5926
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2338 2338
Size: 5926
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5926
XmaI C'CCGG_G
Cuts at: 1870 2483 3251 1870
Size: 613 768 4545
Fragments arranged by size:
4545 768 613
XmnI GAAnn'nnTTC
Cuts at: 4055 4055
Size: 5926
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3734 276
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI
SfoI SrfI SwaI