Restriction map for
pFN21AE1543
(Circular) (Six-base) MAPSORT of: pFN21AE1543.seq Check: 8669 from: 1 to: 6124
pFN21AE1543 6124 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3273 3273
Size: 6124
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3934 278
Size: 53 83 186 936 2398 2468
Fragments arranged by size:
2468 2398 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2760 2874 3467 1937
Size: 823 114 593 4594
Fragments arranged by size:
4594 823 593 114
Acc65I G'GTAC_C
Cuts at: 3445 3445
Size: 6124
AclI AA'CG_TT
Cuts at: 1563 4251 4624 1563
Size: 2688 373 3063
Fragments arranged by size:
3063 2688 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4187 5571 5839 1632
Size: 2555 1384 268 1917
Fragments arranged by size:
2555 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5927
AflIII A'CryG_T
Cuts at: 1203 2350 5023 6000 1203
Size: 1147 2673 977 1327
Fragments arranged by size:
2673 1327 1147 977
AgeI A'CCGG_T
Cuts at: 4955 4955
Size: 6124
AhdI GACnn_n'nnGTC
Cuts at: 4853 4853
Size: 6124
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6124
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6124
AlwNI CAG_nnn'CTG
Cuts at: 3867 5439 3867
Size: 1572 4552
ApaI G_GGCC'C
Cuts at: 3109 3109
Size: 6124
ApaLI G'TGCA_C
Cuts at: 4181 5337 4181
Size: 1156 4968
ApoI r'AATT_y
Cuts at: 1493 3433 3659 3811 5920 1493
Size: 1940 226 152 2109 1697
Fragments arranged by size:
2109 1940 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4678 160
Size: 872 3646 1606
Fragments arranged by size:
3646 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
AvaI C'yCGr_G
Cuts at: 1870 1943 3449 1870
Size: 73 1506 4545
Fragments arranged by size:
4545 1506 73
BamHI G'GATC_C
Cuts at: 3454 3821 3454
Size: 367 5757
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2161 2604 3347 3445 4900
Size: 325 217 627 374 443 743 98 1455
Cuts at: 4900 618
Size: 1842
Fragments arranged by size:
1842 1455 743 627 443 374 325 217
98
BanII G_rGCy'C
Cuts at: 1391 2073 3109 3243 3443 1391
Size: 682 1036 134 200 4072
Fragments arranged by size:
4072 1036 682 200 134
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2872 928
Size: 971 973 4180
Fragments arranged by size:
4180 973 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2348 2382 2759 2793 4315
Size: 34 50 34 327 34 377 34 1522
Cuts at: 4315 4349 1903
Size: 34 3678
Fragments arranged by size:
3678 1522 377 327 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2271 4016 5226 2271
Size: 1745 1210 3169
Fragments arranged by size:
3169 1745 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6124
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3356 4735
Size: 107 122 71 631 605 337 1347 1379
Cuts at: 4735 136
Size: 1525
Fragments arranged by size:
1525 1379 1347 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 6119 6119
Size: 6124
BlpI GC'TnA_GC
Cuts at: 3541 3541
Size: 6124
Bme1580I G_kGCm'C
Cuts at: 1790 2133 2329 3109 4185 5341 1790
Size: 343 196 780 1076 1156 2573
Fragments arranged by size:
2573 1156 1076 780 343 196
BmgBI CAC'GTC
Cuts at: 1318 2128 1318
Size: 810 5314
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2413 2671 2864
Size: 416 53 83 399 24 996 258 193
Cuts at: 2864 3103 3141 3855 4813 442
Size: 239 38 714 958 1753
Fragments arranged by size:
1753 996 958 714 416 399 258 239
193 83 53 38 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2490 2588 3322 3363 4784 1239
Size: 1251 98 734 41 1421 2579
Fragments arranged by size:
2579 1421 1251 734 98 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2057 2141 4249 5114 5412 2057
Size: 84 2108 865 298 2769
Fragments arranged by size:
2769 2108 865 298 84
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3011 4787 882
Size: 617 1512 1776 2219
Fragments arranged by size:
2219 1776 1512 617
BsaAI yAC'GTr
Cuts at: 493 1204 3067 3915 493
Size: 711 1863 848 2702
Fragments arranged by size:
2702 1863 848 711
BsaBI GATnn'nnATC
Cuts at: 2454 6118 2454
Size: 3664 2460
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3931 4313 4948 275
Size: 53 83 186 936 2398 382 635 1451
Fragments arranged by size:
2398 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2331 4557 4955 5229 5376 5787 2331
Size: 2226 398 274 147 411 2668
Fragments arranged by size:
2668 2226 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5899
Fragments arranged by size:
5899 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6124
BseYI C'CCAG_C
Cuts at: 3219 3365 5327 5823 3219
Size: 146 1962 496 3520
Fragments arranged by size:
3520 1962 496 146
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2210 2675 1163
Size: 573 474 465 4612
Fragments arranged by size:
4612 573 474 465
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2248 3574 4335 5363 1540
Size: 275 186 247 1326 761 1028 2301
Fragments arranged by size:
2301 1326 1028 761 275 247 186
BsiHKAI G_wGCw'C
Cuts at: 2073 2361 2741 3243 4185 4270 5341 2073
Size: 288 380 502 942 85 1071 2856
Fragments arranged by size:
2856 1071 942 502 380 288 85
BsmI GAATG_Cn'
Cuts at: 2182 3635 3728 2182
Size: 1453 93 4578
Fragments arranged by size:
4578 1453 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2133 2329 2361 2741 3109 3243
Size: 399 283 60 196 32 380 368 134
Cuts at: 3243 3443 4185 4270 5341 1391
Size: 200 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 380 368 283 200
196 134 85 60 32
BspEI T'CCGG_A
Cuts at: 5787 5787
Size: 6124
BspHI T'CATG_A
Cuts at: 2644 4013 5743 2644
Size: 1369 1730 3025
Fragments arranged by size:
3025 1730 1369
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3029 3273 3479 844
Size: 2185 244 206 3489
Fragments arranged by size:
3489 2185 244 206
BsrBI CCG'CTC
Cuts at: 1394 1823 4011 1394
Size: 429 2188 3507
Fragments arranged by size:
3507 2188 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2713 3320 4619 4793 65
Size: 1183 1465 607 1299 174 1396
Fragments arranged by size:
1465 1396 1299 1183 607 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2103 2460 4768 4955 1700
Size: 110 293 357 2308 187 2869
Fragments arranged by size:
2869 2308 357 293 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6124
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6124
BssSI C'ACGA_G
Cuts at: 4184 5196 4184
Size: 1012 5112
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2563 794
Size: 1769 4355
BstBI TT'CG_AA
Cuts at: 4928 4928
Size: 6124
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6124
BstXI CCAn_nnnn'nTGG
Cuts at: 3116 3122 3209 3388 3116
Size: 6 87 179 5852
Fragments arranged by size:
5852 179 87 6
BstYI r'GATC_y
Cuts at: 1972 3454 3821 4206 4223 5675 5761 5773 6119
Size: 1482 367 385 17 1452 86 12 346
Cuts at: 6119 1972
Size: 1977
Fragments arranged by size:
1977 1482 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2064 2791 3115 3121 513
Size: 552 999 727 324 6 3516
Fragments arranged by size:
3516 999 727 552 324 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5385
Fragments arranged by size:
5385 683 56
BtsI GCAGTG_nn'
Cuts at: 2573 2598 3640 4434 4454 2573
Size: 25 1042 794 20 4243
Fragments arranged by size:
4243 1042 794 25 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6124
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3302 3337 529
Size: 35 2738 35 3316
Fragments arranged by size:
3316 2738 35 35
DraI TTT'AAA
Cuts at: 3428 3428
Size: 6124
DraIII CAC_nnn'GTG
Cuts at: 3212 3212
Size: 6124
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2377 2516 5131 809
Size: 507 1061 139 2615 1802
Fragments arranged by size:
2615 1802 1061 507 139
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2101 2481 2794
Size: 54 1425 50 275 18 271 380 313
Cuts at: 2794 3118 3199 3571 4460 6089 8
Size: 324 81 372 889 1629 43
Fragments arranged by size:
1629 1425 889 380 372 324 313 275
271 81 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3571 1537
Size: 275 1759 4090
Fragments arranged by size:
4090 1759 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2753 4054 1353
Size: 1400 1301 3423
Fragments arranged by size:
3423 1400 1301
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3176 4711 5085 5231 387
Size: 1064 138 1587 1535 374 146 1280
Fragments arranged by size:
1587 1535 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2071 3241 2071
Size: 1170 4954
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2490 2588 3322 3363 4187 4784 5571
Size: 393 858 98 734 41 824 597 787
Cuts at: 5571 5839 1239
Size: 268 1524
Fragments arranged by size:
1524 858 824 787 734 597 393 268
98 41
EcoO109I rG'GnC_Cy
Cuts at: 2021 2827 3369 2021
Size: 806 542 4776
Fragments arranged by size:
4776 806 542
EcoRI G'AATT_C
Cuts at: 3433 3811 3433
Size: 378 5746
FseI GG_CCGG'CC
Cuts at: 2107 2107
Size: 6124
FspI TGC'GCA
Cuts at: 2432 4630 2432
Size: 2198 3926
HaeII r_GCGC'y
Cuts at: 1678 3130 5271 1678
Size: 1452 2141 2531
Fragments arranged by size:
2531 2141 1452
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2921 2953 4765 4797 4839
Size: 32 135 32 1349 32 1812 32 42
Cuts at: 4839 4871 1373
Size: 32 2626
Fragments arranged by size:
2626 1812 1349 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3468 3714 669
Size: 988 281 1530 246 3079
Fragments arranged by size:
3079 1530 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6124
HpaI GTT'AAC
Cuts at: 3714 3714
Size: 6124
KpnI G_GTAC'C
Cuts at: 3449 3449
Size: 6124
MfeI C'AATT_G
Cuts at: 3723 3723
Size: 6124
MluI A'CGCG_T
Cuts at: 6000 6000
Size: 6124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5238 5422 1305
Size: 3933 184 2007
Fragments arranged by size:
3933 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2483 3120 3201 10
Size: 54 1425 343 651 637 81 2933
Fragments arranged by size:
2933 1425 651 637 343 81 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2298 2649 3120 4082 4441 4600 518
Size: 648 1132 351 471 962 359 159 2042
Fragments arranged by size:
2042 1132 962 648 471 359 351 159
MspA1I CmG'CkG
Cuts at: 1606 2682 2716 2972 3223 3282 3341 3540 4217
Size: 1076 34 256 251 59 59 199 677
Cuts at: 4217 5365 5610 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1076 677 256 251 245 199
59 59 34
NaeI GCC'GGC
Cuts at: 2105 2105
Size: 6124
NcoI C'CATG_G
Cuts at: 513 1065 2064 2791 3115 3121 513
Size: 552 999 727 324 6 3516
Fragments arranged by size:
3516 999 727 552 324 6
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6124
NgoMIV G'CCGG_C
Cuts at: 2103 2103
Size: 6124
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6124
NotI GC'GGCC_GC
Cuts at: 3571 3571
Size: 6124
NspI r_CATG'y
Cuts at: 1132 1229 3482 5027 1132
Size: 97 2253 1545 2229
Fragments arranged by size:
2253 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2962 1348
Size: 1614 4510
PciI A'CATG_T
Cuts at: 5023 5023
Size: 6124
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2479 1108
Size: 1371 4753
PmeI GTTT'AAAC
Cuts at: 3428 3428
Size: 6124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2528 2560 4193 4225 5706 5738 2528
Size: 32 1633 32 1481 32 2914
Fragments arranged by size:
2914 1633 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3369 3369
Size: 6124
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6124
PsiI TTA'TAA
Cuts at: 3694 3694
Size: 6124
PspOMI G'GGCC_C
Cuts at: 3105 3105
Size: 6124
PstI C_TGCA'G
Cuts at: 830 2346 3270 3476 830
Size: 1516 924 206 3478
Fragments arranged by size:
3478 1516 924 206
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6124
PvuII CAG'CTG
Cuts at: 2716 2972 3223 3341 2716
Size: 256 251 118 5499
Fragments arranged by size:
5499 256 251 118
SacI G_AGCT'C
Cuts at: 2073 3243 2073
Size: 1170 4954
SalI G'TCGA_C
Cuts at: 1936 3466 1936
Size: 1530 4594
SanDI GG'GwC_CC
Cuts at: 3369 3369
Size: 6124
SbfI CC_TGCA'GG
Cuts at: 3476 3476
Size: 6124
ScaI AGT'ACT
Cuts at: 4372 6084 4372
Size: 1712 4412
SexAI A'CCwGG_T
Cuts at: 3208 3208
Size: 6124
SfcI C'TryA_G
Cuts at: 826 1045 2342 2896 3266 3472 4607 5288 5479
Size: 219 1297 554 370 206 1135 681 191
Cuts at: 5479 826
Size: 1471
Fragments arranged by size:
1471 1297 1135 681 554 370 219 206
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6124
SmaI CCC'GGG
Cuts at: 1872 3451 1872
Size: 1579 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2072 2156 4228 5129 5391 820
Size: 197 926 129 84 2072 901 262 1553
Fragments arranged by size:
2072 1553 926 901 262 197 129 84
SnaBI TAC'GTA
Cuts at: 493 3915 493
Size: 3422 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6124
SphI G_CATG'C
Cuts at: 1132 3482 1132
Size: 2350 3774
SspI AAT'ATT
Cuts at: 5 52 2227 3095 4048 5
Size: 47 2175 868 953 2081
Fragments arranged by size:
2175 2081 953 868 47
StuI AGG'CCT
Cuts at: 1517 2195 2256 3359 1517
Size: 678 61 1103 4282
Fragments arranged by size:
4282 1103 678 61
StyI C'CwwG_G
Cuts at: 513 1065 2064 2791 3115 3121 3420 3563 513
Size: 552 999 727 324 6 299 143 3074
Fragments arranged by size:
3074 999 727 552 324 299 143 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2104 2416 2861 3104 4151 4168 4321
Size: 1255 524 312 445 243 1047 17 153
Cuts at: 4321 4506 4951 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1047 524 445 445 312 243
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4370 6082 96
Size: 274 80 33 51 1443 2393 1712 138
Fragments arranged by size:
2393 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2274 2306 6084 6116 2274
Size: 32 3778 32 2282
Fragments arranged by size:
3778 2282 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6124
XbaI T'CTAG_A
Cuts at: 3460 3460
Size: 6124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2032 2032
Size: 6124
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6124
XmaI C'CCGG_G
Cuts at: 1870 3449 1870
Size: 1579 4545
XmnI GAAnn'nnTTC
Cuts at: 4253 4253
Size: 6124
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3932 276
Size: 53 83 186 936 2398 2468
Fragments arranged by size:
2468 2398 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV
FalI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacII SapI SfiI SfoI
SrfI SwaI