Restriction map for
pFN21AE1541
(Circular) (Six-base) MAPSORT of: pFN21AE1541.seq Check: 2983 from: 1 to: 6130
pFN21AE1541 6130 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3940 278
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2544 3473 1937
Size: 607 929 4594
Fragments arranged by size:
4594 929 607
Acc65I G'GTAC_C
Cuts at: 3451 3451
Size: 6130
AclI AA'CG_TT
Cuts at: 1563 4257 4630 1563
Size: 2694 373 3063
Fragments arranged by size:
3063 2694 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2882 4193 5577 5845 1632
Size: 1250 1311 1384 268 1917
Fragments arranged by size:
1917 1384 1311 1250 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5933
AflIII A'CryG_T
Cuts at: 1203 2821 5029 6006 1203
Size: 1618 2208 977 1327
Fragments arranged by size:
2208 1618 1327 977
AgeI A'CCGG_T
Cuts at: 4961 4961
Size: 6130
AhdI GACnn_n'nnGTC
Cuts at: 4859 4859
Size: 6130
AleI CACnn'nnGTG
Cuts at: 1166 3410 1166
Size: 2244 3886
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2235 1184
Size: 1051 5079
AlwNI CAG_nnn'CTG
Cuts at: 2425 3365 3873 5445 2425
Size: 940 508 1572 3110
Fragments arranged by size:
3110 1572 940 508
ApaLI G'TGCA_C
Cuts at: 4187 5343 4187
Size: 1156 4974
ApoI r'AATT_y
Cuts at: 1493 3271 3353 3439 3665 3817 5926 1493
Size: 1778 82 86 226 152 2109 1697
Fragments arranged by size:
2109 1778 1697 226 152 86 82
AseI AT'TA_AT
Cuts at: 160 1032 2787 4684 160
Size: 872 1755 1897 1606
Fragments arranged by size:
1897 1755 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
AvaI C'yCGr_G
Cuts at: 1870 1943 3455 1870
Size: 73 1512 4545
Fragments arranged by size:
4545 1512 73
BamHI G'GATC_C
Cuts at: 3460 3827 3460
Size: 367 5763
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2320 3451 4906 618
Size: 325 217 627 533 1131 1455 1842
Fragments arranged by size:
1842 1455 1131 627 533 325 217
BanII G_rGCy'C
Cuts at: 1391 2260 3449 1391
Size: 869 1189 4072
Fragments arranged by size:
4072 1189 869
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2846 928
Size: 971 947 4212
Fragments arranged by size:
4212 971 947
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2130 2164 4321 4355 1903
Size: 34 193 34 2157 34 3678
Fragments arranged by size:
3678 2157 193 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2426 4022 5232 2426
Size: 1596 1210 3324
Fragments arranged by size:
3324 1596 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6130
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2864 4741 136
Size: 107 122 71 631 605 1192 1877 1525
Fragments arranged by size:
1877 1525 1192 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6125 6125
Size: 6130
BlpI GC'TnA_GC
Cuts at: 2767 3547 2767
Size: 780 5350
Bme1580I G_kGCm'C
Cuts at: 1790 4191 5347 1790
Size: 2401 1156 2573
Fragments arranged by size:
2573 2401 1156
BmgBI CAC'GTC
Cuts at: 1318 2209 3157 1318
Size: 891 948 4291
Fragments arranged by size:
4291 948 891
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2557 2720 3353
Size: 416 53 83 399 24 1140 163 633
Cuts at: 3353 3861 4819 442
Size: 508 958 1753
Fragments arranged by size:
1753 1140 958 633 508 416 399 163
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2078 2644 2996 3447 4790 1239
Size: 839 566 352 451 1343 2579
Fragments arranged by size:
2579 1343 839 566 451 352
Bpu10I CC'TnA_GC
Cuts at: 3263 3366 3263
Size: 103 6027
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4255 5120 5418 4255
Size: 865 298 4967
Fragments arranged by size:
4967 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4793 882
Size: 617 3294 2219
Fragments arranged by size:
3294 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3921 493
Size: 711 2717 2702
Fragments arranged by size:
2717 2702 711
BsaBI GATnn'nnATC
Cuts at: 6124 6124
Size: 6130
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3937 4319 4954 275
Size: 53 83 186 936 2404 382 635 1451
Fragments arranged by size:
2404 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2612 4563 4961 5235 5382 5793 2612
Size: 1951 398 274 147 411 2949
Fragments arranged by size:
2949 1951 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5905
Fragments arranged by size:
5905 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2685 2709 1186
Size: 1499 24 4607
Fragments arranged by size:
4607 1499 24
BseYI C'CCAG_C
Cuts at: 2430 5333 5829 2430
Size: 2903 496 2731
Fragments arranged by size:
2903 2731 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2156 2231 2892 1163
Size: 573 420 75 661 4401
Fragments arranged by size:
4401 661 573 420 75
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3580 4341 5369 1540
Size: 275 186 1579 761 1028 2301
Fragments arranged by size:
2301 1579 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2260 4191 4276 5347 2260
Size: 1931 85 1071 3043
Fragments arranged by size:
3043 1931 1071 85
BsmI GAATG_Cn'
Cuts at: 3641 3734 3641
Size: 93 6037
BsmBI CGTCTCn'nnnn_
Cuts at: 2199 2199
Size: 6130
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2260 3449 4191 4276 5347 1391
Size: 399 470 1189 742 85 1071 2174
Fragments arranged by size:
2174 1189 1071 742 470 399 85
BspEI T'CCGG_A
Cuts at: 5793 5793
Size: 6130
BspHI T'CATG_A
Cuts at: 4019 5749 4019
Size: 1730 4400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3485 844
Size: 2641 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2658 2766 4017 1394
Size: 429 835 108 1251 3507
Fragments arranged by size:
3507 1251 835 429 108
BsrDI GCAATG_nn'
Cuts at: 65 1248 3083 4625 4799 65
Size: 1183 1835 1542 174 1396
Fragments arranged by size:
1835 1542 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4774 4961 1700
Size: 110 2964 187 2869
Fragments arranged by size:
2964 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6130
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6130
BssSI C'ACGA_G
Cuts at: 4190 5202 4190
Size: 1012 5118
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6130
BstBI TT'CG_AA
Cuts at: 4934 4934
Size: 6130
BstEII G'GTnAC_C
Cuts at: 1184 3403 1184
Size: 2219 3911
BstXI CCAn_nnnn'nTGG
Cuts at: 2617 2617
Size: 6130
BstYI r'GATC_y
Cuts at: 1972 3460 3827 4212 4229 5681 5767 5779 6125
Size: 1488 367 385 17 1452 86 12 346
Cuts at: 6125 1972
Size: 1977
Fragments arranged by size:
1977 1488 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2089 513
Size: 552 939 85 4554
Fragments arranged by size:
4554 939 552 85
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5391
Fragments arranged by size:
5391 683 56
BtsI GCAGTG_nn'
Cuts at: 2052 2893 3646 4440 4460 2052
Size: 841 753 794 20 3722
Fragments arranged by size:
3722 841 794 753 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6095
DraI TTT'AAA
Cuts at: 3434 3434
Size: 6130
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5137 809
Size: 507 3821 1802
Fragments arranged by size:
3821 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3577 4466 6095
Size: 54 1425 50 275 18 1747 889 1629
Cuts at: 6095 8
Size: 43
Fragments arranged by size:
1747 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3577 1537
Size: 275 1765 4090
Fragments arranged by size:
4090 1765 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4060 1353
Size: 2707 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4717 5091 5237 387
Size: 1064 138 3128 374 146 1280
Fragments arranged by size:
3128 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2258 2258
Size: 6130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2644 2882 2996 3447 4193 4790
Size: 393 446 566 238 114 451 746 597
Cuts at: 4790 5577 5845 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 746 597 566 451 446 393
268 238 114
EcoNI CCTnn'n_nnAGG
Cuts at: 2706 3333 2706
Size: 627 5503
EcoO109I rG'GnC_Cy
Cuts at: 2422 2636 2690 2711 2422
Size: 214 54 21 5841
Fragments arranged by size:
5841 214 54 21
EcoRI G'AATT_C
Cuts at: 3271 3353 3439 3817 3271
Size: 82 86 378 5584
Fragments arranged by size:
5584 378 86 82
FspI TGC'GCA
Cuts at: 4636 4636
Size: 6130
HaeII r_GCGC'y
Cuts at: 1678 5277 1678
Size: 3599 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2051 2083 4771 4803 4845
Size: 32 135 32 479 32 2688 32 42
Cuts at: 4845 4877 1373
Size: 32 2626
Fragments arranged by size:
2688 2626 479 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2285 3076 3474 3720 669
Size: 988 281 347 791 398 246 3079
Fragments arranged by size:
3079 988 791 398 347 281 246
HindIII A'AGCT_T
Cuts at: 748 2303 2556 748
Size: 1555 253 4322
Fragments arranged by size:
4322 1555 253
HpaI GTT'AAC
Cuts at: 3720 3720
Size: 6130
KpnI G_GTAC'C
Cuts at: 3455 3455
Size: 6130
MfeI C'AATT_G
Cuts at: 3729 3729
Size: 6130
MluI A'CGCG_T
Cuts at: 6006 6006
Size: 6130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2229 3042 5244 5428 1305
Size: 924 813 2202 184 2007
Fragments arranged by size:
2202 2007 924 813 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4308
Fragments arranged by size:
4308 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3410 4088 4447 4606 518
Size: 648 2244 678 359 159 2042
Fragments arranged by size:
2244 2042 678 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2402 2963 3546 4223 5371 5616 1606
Size: 796 561 583 677 1148 245 2120
Fragments arranged by size:
2120 1148 796 677 583 561 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4639
Fragments arranged by size:
4639 939 552
NdeI CA'TA_TG
Cuts at: 387 2131 387
Size: 1744 4386
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6130
NotI GC'GGCC_GC
Cuts at: 3577 3577
Size: 6130
NspI r_CATG'y
Cuts at: 1132 1229 2017 2086 2222 2543 2813 2825 3488
Size: 97 788 69 136 321 270 12 663
Cuts at: 3488 5033 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 788 663 321 270 136 97
69 12
PasI CC'CwG_GG
Cuts at: 1348 2425 1348
Size: 1077 5053
PciI A'CATG_T
Cuts at: 2821 5029 2821
Size: 2208 3922
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2425 1108
Size: 1317 4813
PmeI GTTT'AAAC
Cuts at: 3434 3434
Size: 6130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4199 4231 5712 5744 4199
Size: 32 1481 32 4585
Fragments arranged by size:
4585 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2690 2690
Size: 6130
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6130
PsiI TTA'TAA
Cuts at: 3700 3700
Size: 6130
PstI C_TGCA'G
Cuts at: 830 2586 3482 830
Size: 1756 896 3478
Fragments arranged by size:
3478 1756 896
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6130
PvuII CAG'CTG
Cuts at: 2402 2963 2402
Size: 561 5569
SacI G_AGCT'C
Cuts at: 2260 2260
Size: 6130
SalI G'TCGA_C
Cuts at: 1936 3472 1936
Size: 1536 4594
SbfI CC_TGCA'GG
Cuts at: 3482 3482
Size: 6130
ScaI AGT'ACT
Cuts at: 2177 4378 6090 2177
Size: 2201 1712 2217
Fragments arranged by size:
2217 2201 1712
SexAI A'CCwGG_T
Cuts at: 3021 3021
Size: 6130
SfcI C'TryA_G
Cuts at: 826 1045 2582 3330 3478 4613 5294 5485 826
Size: 219 1537 748 148 1135 681 191 1471
Fragments arranged by size:
1537 1471 1135 748 681 219 191 148
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6130
SmaI CCC'GGG
Cuts at: 1872 3457 1872
Size: 1585 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4234 5135 5397 820
Size: 197 926 2291 901 262 1553
Fragments arranged by size:
2291 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3921 493
Size: 3428 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6130
SphI G_CATG'C
Cuts at: 1132 3488 1132
Size: 2356 3774
SspI AAT'ATT
Cuts at: 5 52 4054 5
Size: 47 4002 2081
Fragments arranged by size:
4002 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6130
StyI C'CwwG_G
Cuts at: 513 1065 2004 3569 513
Size: 552 939 1565 3074
Fragments arranged by size:
3074 1565 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2338 2422 4157 4174 4327 4512 4957
Size: 1255 758 84 1735 17 153 185 445
Cuts at: 4957 325
Size: 1498
Fragments arranged by size:
1735 1498 1255 758 445 185 153 84
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2175 2517 2965
Size: 274 80 33 51 1443 198 342 448
Cuts at: 2965 4376 6088 96
Size: 1411 1712 138
Fragments arranged by size:
1712 1443 1411 448 342 274 198 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6090 6122 6090
Size: 32 6098
Tth111I GACn'n_nGTC
Cuts at: 1316 2549 1316
Size: 1233 4897
XbaI T'CTAG_A
Cuts at: 3466 3466
Size: 6130
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6130
XmaI C'CCGG_G
Cuts at: 1870 3455 1870
Size: 1585 4545
XmnI GAAnn'nnTTC
Cuts at: 4259 4259
Size: 6130
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3938 276
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI