Restriction map for
pFN21AE1539
(Circular) (Six-base) MAPSORT of: pFN21AE1539.seq Check: 7996 from: 1 to: 5644
pFN21AE1539 5644 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3454 278
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2987 1937
Size: 1050 4594
Acc65I G'GTAC_C
Cuts at: 2965 2965
Size: 5644
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2090 2575 3707 5091 5359 1632
Size: 458 485 1132 1384 268 1917
Fragments arranged by size:
1917 1384 1132 485 458 268
AfeI AGC'GCT
Cuts at: 2077 2077
Size: 5644
AflII C'TTAA_G
Cuts at: 820 1017 2164 820
Size: 197 1147 4300
Fragments arranged by size:
4300 1147 197
AflIII A'CryG_T
Cuts at: 1203 2554 4543 5520 1203
Size: 1351 1989 977 1327
Fragments arranged by size:
1989 1351 1327 977
AgeI A'CCGG_T
Cuts at: 4475 4475
Size: 5644
AhdI GACnn_n'nnGTC
Cuts at: 4373 4373
Size: 5644
AleI CACnn'nnGTG
Cuts at: 1166 2309 1166
Size: 1143 4501
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5644
AlwNI CAG_nnn'CTG
Cuts at: 3387 4959 3387
Size: 1572 4072
ApaI G_GGCC'C
Cuts at: 2875 2875
Size: 5644
ApaLI G'TGCA_C
Cuts at: 3701 4857 3701
Size: 1156 4488
ApoI r'AATT_y
Cuts at: 1493 2386 2477 2772 2953 3179 3331 5440 1493
Size: 893 91 295 181 226 152 2109 1697
Fragments arranged by size:
2109 1697 893 295 226 181 152 91
AseI AT'TA_AT
Cuts at: 160 1032 2535 4198 160
Size: 872 1503 1663 1606
Fragments arranged by size:
1663 1606 1503 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2969 1870
Size: 73 1026 4545
Fragments arranged by size:
4545 1026 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2364 2397 2364
Size: 33 5611
BamHI G'GATC_C
Cuts at: 2974 3341 2974
Size: 367 5277
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2965 4420 618
Size: 325 217 627 1178 1455 1842
Fragments arranged by size:
1842 1455 1178 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2875 2963 1391
Size: 1484 88 4072
Fragments arranged by size:
4072 1484 88
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2410 928
Size: 971 511 4162
Fragments arranged by size:
4162 971 511
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2141 2175 3835 3869 1903
Size: 34 50 34 120 34 1660 34 3678
Fragments arranged by size:
3678 1660 120 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3536 4746 3536
Size: 1210 4434
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5644
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4255 136
Size: 107 122 71 631 605 2583 1525
Fragments arranged by size:
2583 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2400 5639 2400
Size: 3239 2405
BlpI GC'TnA_GC
Cuts at: 3061 3061
Size: 5644
Bme1580I G_kGCm'C
Cuts at: 1790 2306 2875 3705 4861 1790
Size: 516 569 830 1156 2573
Fragments arranged by size:
2573 1156 830 569 516
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5644
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3375 4333 442
Size: 416 53 83 399 24 1958 958 1753
Fragments arranged by size:
1958 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2100 2526 4304 1239
Size: 861 426 1778 2579
Fragments arranged by size:
2579 1778 861 426
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3769 4634 4932 3769
Size: 865 298 4481
Fragments arranged by size:
4481 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4307 882
Size: 617 2808 2219
Fragments arranged by size:
2808 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2555 3435 493
Size: 711 1351 880 2702
Fragments arranged by size:
2702 1351 880 711
BsaBI GATnn'nnATC
Cuts at: 5638 5638
Size: 5644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3451 3833 4468 275
Size: 53 83 186 936 1918 382 635 1451
Fragments arranged by size:
1918 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4077 4475 4749 4896 5307 4077
Size: 398 274 147 411 4414
Fragments arranged by size:
4414 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5419
Fragments arranged by size:
5419 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5644
BseYI C'CCAG_C
Cuts at: 4847 5343 4847
Size: 496 5148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2760 1163
Size: 573 1024 4047
Fragments arranged by size:
4047 1024 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2035 3094 3855 4883 1540
Size: 275 186 34 1059 761 1028 2301
Fragments arranged by size:
2301 1059 1028 761 275 186 34
BsiHKAI G_wGCw'C
Cuts at: 3705 3790 4861 3705
Size: 85 1071 4488
Fragments arranged by size:
4488 1071 85
BsmI GAATG_Cn'
Cuts at: 3155 3248 3155
Size: 93 5551
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2306 2875 2963 3705 3790 4861 1391
Size: 399 516 569 88 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 569 516 399 88 85
BspEI T'CCGG_A
Cuts at: 5307 5307
Size: 5644
BspHI T'CATG_A
Cuts at: 3533 5263 3533
Size: 1730 3914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2999 844
Size: 2155 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3531 1394
Size: 429 1708 3507
Fragments arranged by size:
3507 1708 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4139 4313 65
Size: 1183 2891 174 1396
Fragments arranged by size:
2891 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4288 4475 1700
Size: 110 2478 187 2869
Fragments arranged by size:
2869 2478 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5644
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5644
BssSI C'ACGA_G
Cuts at: 3704 4716 3704
Size: 1012 4632
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5644
BstBI TT'CG_AA
Cuts at: 4448 4448
Size: 5644
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5644
BstYI r'GATC_y
Cuts at: 1972 2400 2974 3341 3726 3743 5195 5281 5293
Size: 428 574 367 385 17 1452 86 12
Cuts at: 5293 5639 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 574 428 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2041 2880 513
Size: 552 976 839 3277
Fragments arranged by size:
3277 976 839 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4905
Fragments arranged by size:
4905 683 56
BtsI GCAGTG_nn'
Cuts at: 2748 3160 3954 3974 2748
Size: 412 794 20 4418
Fragments arranged by size:
4418 794 412 20
ClaI AT'CG_AT
Cuts at: 1554 2151 1554
Size: 597 5047
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5609
DraI TTT'AAA
Cuts at: 2731 2948 2731
Size: 217 5427
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4651 809
Size: 507 3335 1802
Fragments arranged by size:
3335 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2032 2313 3091
Size: 54 1425 50 275 18 202 281 778
Cuts at: 3091 3980 5609 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 778 281 275 202 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2032 3091 1537
Size: 275 220 1059 4090
Fragments arranged by size:
4090 1059 275 220
EarI CTCTTCn'nnn_
Cuts at: 1353 2066 2410 3574 1353
Size: 713 344 1164 3423
Fragments arranged by size:
3423 1164 713 344
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4231 4605 4751 387
Size: 1064 138 2642 374 146 1280
Fragments arranged by size:
2642 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2100 2526 2575 3707 4304 5091
Size: 393 458 10 426 49 1132 597 787
Cuts at: 5091 5359 1239
Size: 268 1524
Fragments arranged by size:
1524 1132 787 597 458 426 393 268
49 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2698 2698
Size: 5644
EcoRI G'AATT_C
Cuts at: 2772 2953 3331 2772
Size: 181 378 5085
Fragments arranged by size:
5085 378 181
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2179 2605 2179
Size: 426 5218
FspI TGC'GCA
Cuts at: 4150 4150
Size: 5644
HaeII r_GCGC'y
Cuts at: 1678 2062 2079 4791 1678
Size: 384 17 2712 2531
Fragments arranged by size:
2712 2531 384 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4285 4317 4359 4391 1373
Size: 32 135 32 2713 32 42 32 2626
Fragments arranged by size:
2713 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2988 3234 669
Size: 988 281 1050 246 3079
Fragments arranged by size:
3079 1050 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5644
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KpnI G_GTAC'C
Cuts at: 2969 2969
Size: 5644
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2173 2512 4758 4942 1305
Size: 868 339 2246 184 2007
Fragments arranged by size:
2246 2007 868 339 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2315 10
Size: 54 1425 343 483 3339
Fragments arranged by size:
3339 1425 483 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2309 3602 3961 4120 518
Size: 648 1143 1293 359 159 2042
Fragments arranged by size:
2042 1293 1143 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2043 2545 3060 3737 4885 5130 1606
Size: 437 502 515 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 515 502 437 245
NcoI C'CATG_G
Cuts at: 513 1065 2880 513
Size: 552 1815 3277
Fragments arranged by size:
3277 1815 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5644
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5644
NotI GC'GGCC_GC
Cuts at: 3091 3091
Size: 5644
NspI r_CATG'y
Cuts at: 1132 1229 3002 4547 1132
Size: 97 1773 1545 2229
Fragments arranged by size:
2229 1773 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5644
PciI A'CATG_T
Cuts at: 4543 4543
Size: 5644
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5644
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PmlI CAC'GTG
Cuts at: 2555 2555
Size: 5644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 5226 5258 3713
Size: 32 1481 32 4099
Fragments arranged by size:
4099 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5644
PsiI TTA'TAA
Cuts at: 2798 3214 2798
Size: 416 5228
PspOMI G'GGCC_C
Cuts at: 2871 2871
Size: 5644
PstI C_TGCA'G
Cuts at: 830 2525 2996 830
Size: 1695 471 3478
Fragments arranged by size:
3478 1695 471
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5644
PvuII CAG'CTG
Cuts at: 2545 2545
Size: 5644
SacII CC_GC'GG
Cuts at: 2044 2044
Size: 5644
SalI G'TCGA_C
Cuts at: 1936 2986 1936
Size: 1050 4594
SapI GCTCTTCn'nnn_
Cuts at: 2066 2066
Size: 5644
SbfI CC_TGCA'GG
Cuts at: 2996 2996
Size: 5644
ScaI AGT'ACT
Cuts at: 2331 3892 5604 2331
Size: 1561 1712 2371
Fragments arranged by size:
2371 1712 1561
SfcI C'TryA_G
Cuts at: 826 1045 2521 2767 2992 4127 4808 4999 826
Size: 219 1476 246 225 1135 681 191 1471
Fragments arranged by size:
1476 1471 1135 681 246 225 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2971 1872
Size: 1099 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2164 3748 4649 4911 820
Size: 197 926 221 1584 901 262 1553
Fragments arranged by size:
1584 1553 926 901 262 221 197
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5644
SphI G_CATG'C
Cuts at: 1132 3002 1132
Size: 1870 3774
SspI AAT'ATT
Cuts at: 5 52 2794 3568 5
Size: 47 2742 774 2081
Fragments arranged by size:
2742 2081 774 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5644
StyI C'CwwG_G
Cuts at: 513 1065 2267 2604 2880 3083 513
Size: 552 1202 337 276 203 3074
Fragments arranged by size:
3074 1202 552 337 276 203
SwaI ATTT'AAAT
Cuts at: 2731 2731
Size: 5644
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2327 2668 3671 3688 3841 4026 4471
Size: 1255 747 341 1003 17 153 185 445
Cuts at: 4471 325
Size: 1498
Fragments arranged by size:
1498 1255 1003 747 445 341 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2329 3890 5602
Size: 274 80 33 51 1443 352 1561 1712
Cuts at: 5602 96
Size: 138
Fragments arranged by size:
1712 1561 1443 352 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5604 5636 5604
Size: 32 5612
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5644
XbaI T'CTAG_A
Cuts at: 2980 2980
Size: 5644
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5644
XmaI C'CCGG_G
Cuts at: 1870 2969 1870
Size: 1099 4545
XmnI GAAnn'nnTTC
Cuts at: 2588 2931 3773 2588
Size: 343 842 4459
Fragments arranged by size:
4459 842 343
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3452 276
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII
SacI SanDI SexAI SfiI SfoI SrfI XcmI