Restriction map for
pFN21AE1537
(Circular) (Six-base) MAPSORT of: pFN21AE1537.seq Check: 7587 from: 1 to: 7441
pFN21AE1537 7441 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2146 2146
Size: 7441
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4397 5251 278
Size: 53 83 186 936 2861 854 2468
Fragments arranged by size:
2861 2468 936 854 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4784 1937
Size: 2847 4594
Acc65I G'GTAC_C
Cuts at: 4762 4762
Size: 7441
AclI AA'CG_TT
Cuts at: 1563 5568 5941 1563
Size: 4005 373 3063
Fragments arranged by size:
4005 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3686 4526 5504 6888 7156 1632
Size: 2054 840 978 1384 268 1917
Fragments arranged by size:
2054 1917 1384 978 840 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7244
AflIII A'CryG_T
Cuts at: 1203 6340 7317 1203
Size: 5137 977 1327
Fragments arranged by size:
5137 1327 977
AgeI A'CCGG_T
Cuts at: 6272 6272
Size: 7441
AhdI GACnn_n'nnGTC
Cuts at: 2719 6170 2719
Size: 3451 3990
AleI CACnn'nnGTG
Cuts at: 1166 3967 1166
Size: 2801 4640
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3799 4127 1184
Size: 2615 328 4498
Fragments arranged by size:
4498 2615 328
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3593 3625 3593
Size: 32 7409
AlwNI CAG_nnn'CTG
Cuts at: 2149 2518 3023 3697 4516 5184 6756 2149
Size: 369 505 674 819 668 1572 2834
Fragments arranged by size:
2834 1572 819 674 668 505 369
ApaI G_GGCC'C
Cuts at: 2270 3065 3962 2270
Size: 795 897 5749
Fragments arranged by size:
5749 897 795
ApaLI G'TGCA_C
Cuts at: 5498 6654 5498
Size: 1156 6285
ApoI r'AATT_y
Cuts at: 1493 3795 4673 4750 4976 5128 7237 1493
Size: 2302 878 77 226 152 2109 1697
Fragments arranged by size:
2302 2109 1697 878 226 152 77
AseI AT'TA_AT
Cuts at: 160 1032 5995 160
Size: 872 4963 1606
Fragments arranged by size:
4963 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7441
AvaI C'yCGr_G
Cuts at: 1870 1943 2019 2568 2931 3128 4333 4344 4460
Size: 73 76 549 363 197 1205 11 116
Cuts at: 4460 4554 4766 1870
Size: 94 212 4545
Fragments arranged by size:
4545 1205 549 363 212 197 116 94
76 73 11
BamHI G'GATC_C
Cuts at: 4283 4771 5138 4283
Size: 488 367 6586
Fragments arranged by size:
6586 488 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2166 2338 3085 3648 3864
Size: 325 217 627 379 172 747 563 216
Cuts at: 3864 4762 6217 618
Size: 898 1455 1842
Fragments arranged by size:
1842 1455 898 747 627 563 379 325
217 216 172
BanII G_rGCy'C
Cuts at: 1391 2261 2270 2841 2953 3065 3128 3405 3521
Size: 870 9 571 112 112 63 277 116
Cuts at: 3521 3750 3962 4595 4760 1391
Size: 229 212 633 165 4072
Fragments arranged by size:
4072 870 633 571 277 229 212 165
116 112 112 63 9
BbeI G_GCGC'C
Cuts at: 2170 3089 3868 2170
Size: 919 779 5743
Fragments arranged by size:
5743 919 779
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2016 2327 4539 928
Size: 971 117 311 2212 3830
Fragments arranged by size:
3830 2212 971 311 117
BbvCI CC'TCA_GC
Cuts at: 2082 3513 3842 4358 2082
Size: 1431 329 516 5165
Fragments arranged by size:
5165 1431 516 329
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5632 5666 1903
Size: 34 3695 34 3678
Fragments arranged by size:
3695 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5333 6543 5333
Size: 1210 6231
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7441
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2024 2285 4464
Size: 107 122 71 631 605 352 261 2179
Cuts at: 4464 6052 136
Size: 1588 1525
Fragments arranged by size:
2179 1588 1525 631 605 352 261 122
107 71
BglII A'GATC_T
Cuts at: 7436 7436
Size: 7441
BlpI GC'TnA_GC
Cuts at: 2798 3908 4628 4858 2798
Size: 1110 720 230 5381
Fragments arranged by size:
5381 1110 720 230
Bme1580I G_kGCm'C
Cuts at: 1790 2270 3065 3962 5502 6658 1790
Size: 480 795 897 1540 1156 2573
Fragments arranged by size:
2573 1540 1156 897 795 480
BmgBI CAC'GTC
Cuts at: 1318 3487 3808 3969 1318
Size: 2169 321 161 4790
Fragments arranged by size:
4790 2169 321 161
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2273 5172 6130
Size: 416 53 83 399 24 856 2899 958
Cuts at: 6130 442
Size: 1753
Fragments arranged by size:
2899 1753 958 856 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7441
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2134 4370 2134
Size: 2236 5205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 2171 2336 2486 2549 2627 2991 3056
Size: 890 42 165 150 63 78 364 65
Cuts at: 3056 3095 3156 3188 4022 4383 4676 6101 1239
Size: 39 61 32 834 361 293 1425 2579
Fragments arranged by size:
2579 1425 890 834 364 361 293 165
150 78 65 63 61 42 39 32
Bpu10I CC'TnA_GC
Cuts at: 2082 3513 3842 4317 4358 4589 2082
Size: 1431 329 475 41 231 4934
Fragments arranged by size:
4934 1431 475 329 231 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3833 4049 5566 6431 6729 3833
Size: 216 1517 865 298 4545
Fragments arranged by size:
4545 1517 865 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4634 4681 6104 882
Size: 617 3135 47 1423 2219
Fragments arranged by size:
3135 2219 1423 617 47
BsaAI yAC'GTr
Cuts at: 493 1204 2200 2581 3298 5232 493
Size: 711 996 381 717 1934 2702
Fragments arranged by size:
2702 1934 996 717 711 381
BsaBI GATnn'nnATC
Cuts at: 7435 7435
Size: 7441
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2167 2501 3027 3086
Size: 53 83 186 936 634 334 526 59
Cuts at: 3086 3865 4088 4394 5248 5630 6265 275
Size: 779 223 306 854 382 635 1451
Fragments arranged by size:
1451 936 854 779 635 634 526 382
334 306 223 186 83 59 53
BsaWI w'CCGG_w
Cuts at: 2739 2904 2937 5874 6272 6546 6693 7104 2739
Size: 165 33 2937 398 274 147 411 3076
Fragments arranged by size:
3076 2937 411 398 274 165 147 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2378 2408 1178
Size: 30 165 30 975 30 6211
Fragments arranged by size:
6211 975 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2624 3383 3488 3529 4346 4542 4652 4673
Size: 1438 759 105 41 817 196 110 21
Cuts at: 4673 4682 1186
Size: 9 3945
Fragments arranged by size:
3945 1438 817 759 196 110 105 41
21 9
BseYI C'CCAG_C
Cuts at: 2170 2254 2523 3040 3389 4328 4594 4725 6644
Size: 84 269 517 349 939 266 131 1919
Cuts at: 6644 7140 2170
Size: 496 2471
Fragments arranged by size:
2471 1919 939 517 496 349 269 266
131 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2441 2966 3209 3302 3644 4720 1163
Size: 573 705 525 243 93 342 1076 3884
Fragments arranged by size:
3884 1076 705 573 525 342 243 93
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2768 3161 3433 4891 5652 6680
Size: 275 186 767 393 272 1458 761 1028
Cuts at: 6680 1540
Size: 2301
Fragments arranged by size:
2301 1458 1028 767 761 393 275 272
186
BsiHKAI G_wGCw'C
Cuts at: 3405 4264 5502 5587 6658 3405
Size: 859 1238 85 1071 4188
Fragments arranged by size:
4188 1238 1071 859 85
BsmI GAATG_Cn'
Cuts at: 3353 3415 4952 5045 3353
Size: 62 1537 93 5749
Fragments arranged by size:
5749 1537 93 62
BsmBI CGTCTCn'nnnn_
Cuts at: 3477 4385 4401 3477
Size: 908 16 6517
Fragments arranged by size:
6517 908 16
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2261 2270 2841 2953 3065 3128 3405
Size: 399 471 9 571 112 112 63 277
Cuts at: 3405 3521 3750 3962 4264 4595 4760 5502 5587
Size: 116 229 212 302 331 165 742 85
Cuts at: 5587 6658 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 571 471 399 331 302
277 229 212 165 116 112 112 85
63 9
BspEI T'CCGG_A
Cuts at: 2739 7104 2739
Size: 4365 3076
BspHI T'CATG_A
Cuts at: 5330 7060 5330
Size: 1730 5711
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2146 2434 3846 4032 4632 4796 844
Size: 1302 288 1412 186 600 164 3489
Fragments arranged by size:
3489 1412 1302 600 288 186 164
BsrBI CCG'CTC
Cuts at: 1394 1823 2575 2590 4054 4246 5328 1394
Size: 429 752 15 1464 192 1082 3507
Fragments arranged by size:
3507 1464 1082 752 429 192 15
BsrDI GCAATG_nn'
Cuts at: 65 1248 4384 5936 6110 65
Size: 1183 3136 1552 174 1396
Fragments arranged by size:
3136 1552 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2341 2457 2760 6085 6272 1700
Size: 110 531 116 303 3325 187 2869
Fragments arranged by size:
3325 2869 531 303 187 116 110
BsrGI T'GTAC_A
Cuts at: 96 2676 96
Size: 2580 4861
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7441
BssSI C'ACGA_G
Cuts at: 3953 5501 6513 3953
Size: 1548 1012 4881
Fragments arranged by size:
4881 1548 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3232 4009 794
Size: 2438 777 4226
Fragments arranged by size:
4226 2438 777
BstBI TT'CG_AA
Cuts at: 6245 6245
Size: 7441
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7441
BstXI CCAn_nnnn'nTGG
Cuts at: 3969 3969
Size: 7441
BstYI r'GATC_y
Cuts at: 1972 4283 4771 5138 5523 5540 6992 7078 7090
Size: 2311 488 367 385 17 1452 86 12
Cuts at: 7090 7436 1972
Size: 346 1977
Fragments arranged by size:
2311 1977 1452 488 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2451 2655 3339 3644 3681 4479
Size: 552 939 447 204 684 305 37 798
Cuts at: 4479 513
Size: 3475
Fragments arranged by size:
3475 939 798 684 552 447 305 204
37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6702
Fragments arranged by size:
6702 683 56
BtsI GCAGTG_nn'
Cuts at: 3999 4957 5751 5771 3999
Size: 958 794 20 5669
Fragments arranged by size:
5669 958 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7441
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7406
DraI TTT'AAA
Cuts at: 4745 4745
Size: 7441
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6448 809
Size: 507 5132 1802
Fragments arranged by size:
5132 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4158 4384 4888
Size: 54 1425 50 275 18 2328 226 504
Cuts at: 4888 5777 7406 8
Size: 889 1629 43
Fragments arranged by size:
2328 1629 1425 889 504 275 226 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4888 1537
Size: 275 3076 4090
Fragments arranged by size:
4090 3076 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2118 3177 3332 3738 5371 1353
Size: 765 1059 155 406 1633 3423
Fragments arranged by size:
3423 1633 1059 765 406 155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2600 4129 4541 6028 6402 6548
Size: 1064 138 1011 1529 412 1487 374 146
Cuts at: 6548 387
Size: 1280
Fragments arranged by size:
1529 1487 1280 1064 1011 412 374 146
138
EcoICRI GAG'CTC
Cuts at: 3403 3403
Size: 7441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2171 2336 2486 2549 2627 2991
Size: 393 497 42 165 150 63 78 364
Cuts at: 2991 3056 3095 3156 3188 3686 4022 4383 4526
Size: 65 39 61 32 498 336 361 143
Cuts at: 4526 4676 5504 6101 6888 7156 1239
Size: 150 828 597 787 268 1524
Fragments arranged by size:
1524 828 787 597 498 497 393 364
361 336 268 165 150 150 143 78
65 63 61 42 39 32
EcoNI CCTnn'n_nnAGG
Cuts at: 2153 3470 2153
Size: 1317 6124
EcoO109I rG'GnC_Cy
Cuts at: 2039 2222 2267 3061 3601 3835 3958 4457 4547
Size: 183 45 794 540 234 123 499 90
Cuts at: 4547 4570 2039
Size: 23 4910
Fragments arranged by size:
4910 794 540 499 234 183 123 90
45 23
EcoRI G'AATT_C
Cuts at: 4750 5128 4750
Size: 378 7063
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2305 2305
Size: 7441
FspI TGC'GCA
Cuts at: 3302 5947 3302
Size: 2645 4796
FspAI rTGC'GCAy
Cuts at: 3302 3302
Size: 7441
HaeII r_GCGC'y
Cuts at: 1678 2170 3089 3868 4045 6588 1678
Size: 492 919 779 177 2543 2531
Fragments arranged by size:
2543 2531 919 779 492 177
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2378 2410 2705 2737 2914
Size: 32 135 32 806 32 295 32 177
Cuts at: 2914 2946 6082 6114 6156 6188 1373
Size: 32 3136 32 42 32 2626
Fragments arranged by size:
3136 2626 806 295 177 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2662 3811 4785 5031 669
Size: 988 281 724 1149 974 246 3079
Fragments arranged by size:
3079 1149 988 974 724 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7441
HpaI GTT'AAC
Cuts at: 5031 5031
Size: 7441
KasI G'GCGC_C
Cuts at: 2166 3085 3864 2166
Size: 919 779 5743
Fragments arranged by size:
5743 919 779
KpnI G_GTAC'C
Cuts at: 4766 4766
Size: 7441
MfeI C'AATT_G
Cuts at: 5040 5040
Size: 7441
MluI A'CGCG_T
Cuts at: 7317 7317
Size: 7441
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2748 4221 4644 6555 6739 1305
Size: 1443 1473 423 1911 184 2007
Fragments arranged by size:
2007 1911 1473 1443 423 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4160 10
Size: 54 1425 343 2328 3291
Fragments arranged by size:
3291 2328 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2495 3410 3686 3967 5399 5758 5917
Size: 648 1329 915 276 281 1432 359 159
Cuts at: 5917 518
Size: 2042
Fragments arranged by size:
2042 1432 1329 915 648 359 281 276
159
MspA1I CmG'CkG
Cuts at: 1606 2327 2410 2464 2527 2823 3646 3664 3694
Size: 721 83 54 63 296 823 18 30
Cuts at: 3694 3862 4403 4654 4857 5534 6682 6927 1606
Size: 168 541 251 203 677 1148 245 2120
Fragments arranged by size:
2120 1148 823 721 677 541 296 251
245 203 168 83 63 54 30 18
NaeI GCC'GGC
Cuts at: 2459 2459
Size: 7441
NarI GG'CG_CC
Cuts at: 2167 3086 3865 2167
Size: 919 779 5743
Fragments arranged by size:
5743 919 779
NcoI C'CATG_G
Cuts at: 513 1065 2004 3339 513
Size: 552 939 1335 4615
Fragments arranged by size:
4615 1335 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7441
NgoMIV G'CCGG_C
Cuts at: 2457 2457
Size: 7441
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7441
NotI GC'GGCC_GC
Cuts at: 4888 4888
Size: 7441
NspI r_CATG'y
Cuts at: 1132 1229 2246 2254 2683 3307 4007 4799 6344
Size: 97 1017 8 429 624 700 792 1545
Cuts at: 6344 1132
Size: 2229
Fragments arranged by size:
2229 1545 1017 792 700 624 429 97
8
PasI CC'CwG_GG
Cuts at: 1348 2505 4574 1348
Size: 1157 2069 4215
Fragments arranged by size:
4215 2069 1157
PciI A'CATG_T
Cuts at: 6340 6340
Size: 7441
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2149 2182 3340 1108
Size: 1041 33 1158 5209
Fragments arranged by size:
5209 1158 1041 33
PfoI T'CCnGG_A
Cuts at: 2292 2313 2808 4614 2292
Size: 21 495 1806 5119
Fragments arranged by size:
5119 1806 495 21
PmeI GTTT'AAAC
Cuts at: 4745 4745
Size: 7441
PmlI CAC'GTG
Cuts at: 2200 2581 3298 2200
Size: 381 717 6343
Fragments arranged by size:
6343 717 381
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4341 4373 5510 5542 7023 7055 4341
Size: 32 1137 32 1481 32 4727
Fragments arranged by size:
4727 1481 1137 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2222 3601 3835 4570 2222
Size: 1379 234 735 5093
Fragments arranged by size:
5093 1379 735 234
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7441
PsiI TTA'TAA
Cuts at: 5011 5011
Size: 7441
PspOMI G'GGCC_C
Cuts at: 2266 3061 3958 2266
Size: 795 897 5749
Fragments arranged by size:
5749 897 795
PstI C_TGCA'G
Cuts at: 830 2196 2211 3378 3554 3663 3723 4451 4793
Size: 1366 15 1167 176 109 60 728 342
Cuts at: 4793 830
Size: 3478
Fragments arranged by size:
3478 1366 1167 728 342 176 109 60
15
PvuI CG_AT'CG
Cuts at: 2001 3433 2001
Size: 1432 6009
PvuII CAG'CTG
Cuts at: 2464 2527 3664 4403 4654 2464
Size: 63 1137 739 251 5251
Fragments arranged by size:
5251 1137 739 251 63
RsrII CG'GwC_CG
Cuts at: 4413 4413
Size: 7441
SacI G_AGCT'C
Cuts at: 3405 3405
Size: 7441
SacII CC_GC'GG
Cuts at: 3647 3647
Size: 7441
SalI G'TCGA_C
Cuts at: 1936 4783 1936
Size: 2847 4594
SanDI GG'GwC_CC
Cuts at: 4570 4570
Size: 7441
SapI GCTCTTCn'nnn_
Cuts at: 2118 3177 3738 2118
Size: 1059 561 5821
Fragments arranged by size:
5821 1059 561
SbfI CC_TGCA'GG
Cuts at: 4793 4793
Size: 7441
ScaI AGT'ACT
Cuts at: 5689 7401 5689
Size: 1712 5729
SexAI A'CCwGG_T
Cuts at: 3016 3016
Size: 7441
SfcI C'TryA_G
Cuts at: 826 1045 2192 2207 2898 3374 3550 3659 3719
Size: 219 1147 15 691 476 176 109 60
Cuts at: 3719 4447 4789 5924 6605 6796 826
Size: 728 342 1135 681 191 1471
Fragments arranged by size:
1471 1147 1135 728 691 681 476 342
219 191 176 109 60 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 2285 4464 2285
Size: 2179 5262
SfoI GGC'GCC
Cuts at: 2168 3087 3866 2168
Size: 919 779 5743
Fragments arranged by size:
5743 919 779
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7441
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7441
SmaI CCC'GGG
Cuts at: 1872 2570 4768 1872
Size: 698 2198 4545
Fragments arranged by size:
4545 2198 698
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3848 4064 4554 5545 6446 6708
Size: 197 926 1905 216 490 991 901 262
Cuts at: 6708 820
Size: 1553
Fragments arranged by size:
1905 1553 991 926 901 490 262 216
197
SnaBI TAC'GTA
Cuts at: 493 5232 493
Size: 4739 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7441
SphI G_CATG'C
Cuts at: 1132 2246 3307 4799 1132
Size: 1114 1061 1492 3774
Fragments arranged by size:
3774 1492 1114 1061
SspI AAT'ATT
Cuts at: 5 52 5365 5
Size: 47 5313 2081
Fragments arranged by size:
5313 2081 47
StuI AGG'CCT
Cuts at: 1517 2288 1517
Size: 771 6670
StyI C'CwwG_G
Cuts at: 513 1065 2004 2271 2689 3081 3339 3363 4880
Size: 552 939 267 418 392 258 24 1517
Cuts at: 4880 513
Size: 3074
Fragments arranged by size:
3074 1517 939 552 418 392 267 258
24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2565 4430 5468 5485 5638 5823 6268
Size: 1255 985 1865 1038 17 153 185 445
Cuts at: 6268 325
Size: 1498
Fragments arranged by size:
1865 1498 1255 1038 985 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2676 5687 7399
Size: 274 80 33 51 1443 699 3011 1712
Cuts at: 7399 96
Size: 138
Fragments arranged by size:
3011 1712 1443 699 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2692 2724 2975 3007 7401 7433 2692
Size: 32 251 32 4394 32 2700
Fragments arranged by size:
4394 2700 251 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4237 1316
Size: 2921 4520
XbaI T'CTAG_A
Cuts at: 4777 4777
Size: 7441
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2488 3943 4651 2488
Size: 1455 708 5278
Fragments arranged by size:
5278 1455 708
XhoI C'TCGA_G
Cuts at: 1943 4554 1943
Size: 2611 4830
XmaI C'CCGG_G
Cuts at: 1870 2568 4766 1870
Size: 698 2198 4545
Fragments arranged by size:
4545 2198 698
XmnI GAAnn'nnTTC
Cuts at: 2288 2735 3383 5570 2288
Size: 447 648 2187 4159
Fragments arranged by size:
4159 2187 648 447
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4395 5249 276
Size: 53 83 186 936 2861 854 2468
Fragments arranged by size:
2861 2468 936 854 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I
DraIII EcoRV FseI NruI NsiI PacI PspXI PsrI
SrfI SwaI