Restriction map for
pFN21AE1535
(Circular) (Six-base) MAPSORT of: pFN21AE1535.seq Check: 5703 from: 1 to: 7036
pFN21AE1535 7036 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4846 278
Size: 53 83 186 936 3310 2468
Fragments arranged by size:
3310 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4379 1937
Size: 2442 4594
Acc65I G'GTAC_C
Cuts at: 4357 4357
Size: 7036
AclI AA'CG_TT
Cuts at: 1563 5163 5536 1563
Size: 3600 373 3063
Fragments arranged by size:
3600 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2300 2312 3251 3766 5099 6483 6751 1632
Size: 668 12 939 515 1333 1384 268 1917
Fragments arranged by size:
1917 1384 1333 939 668 515 268 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6839
AflIII A'CryG_T
Cuts at: 1203 2179 3909 5935 6912 1203
Size: 976 1730 2026 977 1327
Fragments arranged by size:
2026 1730 1327 977 976
AgeI A'CCGG_T
Cuts at: 5867 5867
Size: 7036
AhdI GACnn_n'nnGTC
Cuts at: 5765 5765
Size: 7036
AleI CACnn'nnGTG
Cuts at: 1166 3113 3260 1166
Size: 1947 147 4942
Fragments arranged by size:
4942 1947 147
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7036
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4013 4045 4013
Size: 32 7004
AlwNI CAG_nnn'CTG
Cuts at: 2062 3229 3364 3544 3610 4779 6351 2062
Size: 1167 135 180 66 1169 1572 2747
Fragments arranged by size:
2747 1572 1169 1167 180 135 66
ApaLI G'TGCA_C
Cuts at: 5093 6249 5093
Size: 1156 5880
ApoI r'AATT_y
Cuts at: 1493 2143 2435 4345 4571 4723 6832 1493
Size: 650 292 1910 226 152 2109 1697
Fragments arranged by size:
2109 1910 1697 650 292 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5590 160
Size: 872 4558 1606
Fragments arranged by size:
4558 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7036
AvaI C'yCGr_G
Cuts at: 1870 1943 2708 3444 3754 4361 1870
Size: 73 765 736 310 607 4545
Fragments arranged by size:
4545 765 736 607 310 73
BamHI G'GATC_C
Cuts at: 4366 4733 4366
Size: 367 6669
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2021 2950 4231 4357 5812
Size: 325 217 627 234 929 1281 126 1455
Cuts at: 5812 618
Size: 1842
Fragments arranged by size:
1842 1455 1281 929 627 325 234 217
126
BanII G_rGCy'C
Cuts at: 1391 2373 2476 2646 2886 2982 3041 3809 4355
Size: 982 103 170 240 96 59 768 546
Cuts at: 4355 1391
Size: 4072
Fragments arranged by size:
4072 982 768 546 240 170 103 96
59
BbeI G_GCGC'C
Cuts at: 2954 2954
Size: 7036
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6065
BbvCI CC'TCA_GC
Cuts at: 2493 2902 2961 2493
Size: 409 59 6568
Fragments arranged by size:
6568 409 59
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5227 5261 1903
Size: 34 3290 34 3678
Fragments arranged by size:
3678 3290 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4928 6138 4928
Size: 1210 5826
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7036
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3449 3506 3792
Size: 107 122 71 631 605 1777 57 286
Cuts at: 3792 3932 3950 4034 5647 136
Size: 140 18 84 1613 1525
Fragments arranged by size:
1777 1613 1525 631 605 286 140 122
107 84 71 57 18
BglII A'GATC_T
Cuts at: 2094 7031 2094
Size: 4937 2099
BlpI GC'TnA_GC
Cuts at: 4453 4453
Size: 7036
Bme1580I G_kGCm'C
Cuts at: 1790 2424 3063 3099 3463 5097 6253 1790
Size: 634 639 36 364 1634 1156 2573
Fragments arranged by size:
2573 1634 1156 639 634 364 36
BmgBI CAC'GTC
Cuts at: 1318 2182 2425 1318
Size: 864 243 5929
Fragments arranged by size:
5929 864 243
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2491 3016 3939
Size: 416 53 83 399 24 1074 525 923
Cuts at: 3939 4767 5725 442
Size: 828 958 1753
Fragments arranged by size:
1753 1074 958 923 828 525 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7036
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3160 3758 3160
Size: 598 6438
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 3386 3566 3599 3665 3698 5696 1239
Size: 980 1167 180 33 66 33 1998 2579
Fragments arranged by size:
2579 1998 1167 980 180 66 33 33
Bpu10I CC'TnA_GC
Cuts at: 2493 2902 2961 2976 2493
Size: 409 59 15 6553
Fragments arranged by size:
6553 409 59 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5161 6026 6324 5161
Size: 865 298 5873
Fragments arranged by size:
5873 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5699 882
Size: 617 4200 2219
Fragments arranged by size:
4200 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2859 4827 493
Size: 711 1655 1968 2702
Fragments arranged by size:
2702 1968 1655 711
BsaBI GATnn'nnATC
Cuts at: 7030 7030
Size: 7036
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2951 4843 5225 5860
Size: 53 83 186 936 1418 1892 382 635
Cuts at: 5860 275
Size: 1451
Fragments arranged by size:
1892 1451 1418 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2361 2997 5469 5867 6141 6288 6699 2361
Size: 636 2472 398 274 147 411 2698
Fragments arranged by size:
2698 2472 636 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4013 4032 4043 4062 1178
Size: 30 165 30 2610 19 11 19 4152
Fragments arranged by size:
4152 2610 165 30 30 19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2288 2795 2798 2801 2843 2890 3038 3224
Size: 1102 507 3 3 42 47 148 186
Cuts at: 3224 3518 1186
Size: 294 4704
Fragments arranged by size:
4704 1102 507 294 186 148 47 42
3 3
BseYI C'CCAG_C
Cuts at: 2742 3708 3773 3863 3872 3890 3916 4185 4251
Size: 966 65 90 9 18 26 269 66
Cuts at: 4251 4272 4317 6239 6735 2742
Size: 21 45 1922 496 3043
Fragments arranged by size:
3043 1922 966 496 269 90 66 65
45 26 21 18 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2537 3063 3605 1163
Size: 573 801 526 542 4594
Fragments arranged by size:
4594 801 573 542 526
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3318 4486 5247 6275 1540
Size: 275 186 1317 1168 761 1028 2301
Fragments arranged by size:
2301 1317 1168 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2982 3324 3477 3744 5097 5182 6253 2982
Size: 342 153 267 1353 85 1071 3765
Fragments arranged by size:
3765 1353 1071 342 267 153 85
BsmI GAATG_Cn'
Cuts at: 2504 4547 4640 2504
Size: 2043 93 4900
Fragments arranged by size:
4900 2043 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2039 2759 4179 2039
Size: 720 1420 4896
Fragments arranged by size:
4896 1420 720
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2373 2424 2476 2646 2886 2982 3041
Size: 399 583 51 52 170 240 96 59
Cuts at: 3041 3063 3099 3324 3463 3477 3744 3809 4355
Size: 22 36 225 139 14 267 65 546
Cuts at: 4355 5097 5182 6253 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 583 546 399 267 240
225 170 139 96 85 65 59 52
51 36 22 14
BspEI T'CCGG_A
Cuts at: 2361 6699 2361
Size: 4338 2698
BspHI T'CATG_A
Cuts at: 2242 2406 4146 4925 6655 2242
Size: 164 1740 779 1730 2623
Fragments arranged by size:
2623 1740 1730 779 164
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2295 4391 844
Size: 1451 2096 3489
Fragments arranged by size:
3489 2096 1451
BsrBI CCG'CTC
Cuts at: 1394 1823 3631 4923 1394
Size: 429 1808 1292 3507
Fragments arranged by size:
3507 1808 1292 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5531 5705 65
Size: 1183 4283 174 1396
Fragments arranged by size:
4283 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3073 3791 3838 4297 5680 5867 1700
Size: 110 1263 718 47 459 1383 187 2869
Fragments arranged by size:
2869 1383 1263 718 459 187 110 47
BsrGI T'GTAC_A
Cuts at: 96 3825 96
Size: 3729 3307
BssHII G'CGCG_C
Cuts at: 1925 3673 3675 1925
Size: 1748 2 5286
Fragments arranged by size:
5286 1748 2
BssSI C'ACGA_G
Cuts at: 3218 5096 6108 3218
Size: 1878 1012 4146
Fragments arranged by size:
4146 1878 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2135 794
Size: 1341 5695
BstBI TT'CG_AA
Cuts at: 5840 5840
Size: 7036
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7036
BstXI CCAn_nnnn'nTGG
Cuts at: 3115 3118 4084 3115
Size: 3 966 6067
Fragments arranged by size:
6067 966 3
BstYI r'GATC_y
Cuts at: 1972 2094 2325 3432 3525 4366 4733 5118 5135
Size: 122 231 1107 93 841 367 385 17
Cuts at: 5135 6587 6673 6685 7031 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1107 841 385 367 346 231
122 93 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2268 4083 4113 4266 513
Size: 552 939 264 1815 30 153 3283
Fragments arranged by size:
3283 1815 939 552 264 153 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2463 3453 4183 487
Size: 683 56 1237 990 730 3340
Fragments arranged by size:
3340 1237 990 730 683 56
BtsI GCAGTG_nn'
Cuts at: 2545 3876 4552 5346 5366 2545
Size: 1331 676 794 20 4215
Fragments arranged by size:
4215 1331 794 676 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7036
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7001
DraI TTT'AAA
Cuts at: 4340 4340
Size: 7036
DraIII CAC_nnn'GTG
Cuts at: 3823 3823
Size: 7036
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6043 809
Size: 507 4727 1802
Fragments arranged by size:
4727 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2230 2253 3315
Size: 54 1425 50 275 18 400 23 1062
Cuts at: 3315 3597 4053 4483 5372 7001 8
Size: 282 456 430 889 1629 43
Fragments arranged by size:
1629 1425 1062 889 456 430 400 282
275 54 50 43 23 18
EagI C'GGCC_G
Cuts at: 1537 1812 3315 4483 1537
Size: 275 1503 1168 4090
Fragments arranged by size:
4090 1503 1168 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2288 2935 3269 3335 3465 4966 1353
Size: 935 647 334 66 130 1501 3423
Fragments arranged by size:
3423 1501 935 647 334 130 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2099 2840 2894 5623 5997 6143
Size: 1064 138 510 741 54 2729 374 146
Cuts at: 6143 387
Size: 1280
Fragments arranged by size:
2729 1280 1064 741 510 374 146 138
54
EcoICRI GAG'CTC
Cuts at: 2980 2980
Size: 7036
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 2300 2312 3251 3386 3566 3599
Size: 393 587 81 12 939 135 180 33
Cuts at: 3599 3665 3698 3766 5099 5696 6483 6751 1239
Size: 66 33 68 1333 597 787 268 1524
Fragments arranged by size:
1524 1333 939 787 597 587 393 268
180 135 81 68 66 33 33 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2042 2339 2777 3090 2042
Size: 297 438 313 5988
Fragments arranged by size:
5988 438 313 297
EcoO109I rG'GnC_Cy
Cuts at: 2284 4036 2284
Size: 1752 5284
EcoRI G'AATT_C
Cuts at: 2143 2435 4345 4723 2143
Size: 292 1910 378 4456
Fragments arranged by size:
4456 1910 378 292
FspI TGC'GCA
Cuts at: 5542 5542
Size: 7036
HaeII r_GCGC'y
Cuts at: 1678 2810 2909 2954 3239 3455 3517 3523 3610
Size: 1132 99 45 285 216 62 6 87
Cuts at: 3610 6183 1678
Size: 2573 2531
Fragments arranged by size:
2573 2531 1132 285 216 99 87 62
45 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2066 2098 3130 3162 5677
Size: 32 135 32 494 32 1032 32 2515
Cuts at: 5677 5709 5751 5783 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2515 1032 494 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2164 4380 4626 669
Size: 988 281 226 2216 246 3079
Fragments arranged by size:
3079 2216 988 281 246 226
HindIII A'AGCT_T
Cuts at: 748 3485 748
Size: 2737 4299
HpaI GTT'AAC
Cuts at: 4626 4626
Size: 7036
KasI G'GCGC_C
Cuts at: 2950 2950
Size: 7036
KpnI G_GTAC'C
Cuts at: 4361 4361
Size: 7036
MfeI C'AATT_G
Cuts at: 4635 4635
Size: 7036
MluI A'CGCG_T
Cuts at: 6912 6912
Size: 7036
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2046 6150 6334 1305
Size: 741 4104 184 2007
Fragments arranged by size:
4104 2007 741 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2232 2255 3599 10
Size: 54 1425 343 400 23 1344 3447
Fragments arranged by size:
3447 1425 1344 400 343 54 23
MslI CAynn'nnrTG
Cuts at: 518 1166 2411 3113 3260 4994 5353 5512 518
Size: 648 1245 702 147 1734 359 159 2042
Fragments arranged by size:
2042 1734 1245 702 648 359 159 147
MspA1I CmG'CkG
Cuts at: 1606 2019 2508 2659 2746 2797 3361 3382 3532
Size: 413 489 151 87 51 564 21 150
Cuts at: 3532 3595 3851 4010 4452 5129 6277 6522 1606
Size: 63 256 159 442 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 564 489 442 413 256
245 159 151 150 87 63 51 21
NaeI GCC'GGC
Cuts at: 3075 3793 3840 4299 3075
Size: 718 47 459 5812
Fragments arranged by size:
5812 718 459 47
NarI GG'CG_CC
Cuts at: 2951 2951
Size: 7036
NcoI C'CATG_G
Cuts at: 513 1065 2004 2268 4083 4113 513
Size: 552 939 264 1815 30 3436
Fragments arranged by size:
3436 1815 939 552 264 30
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7036
NgoMIV G'CCGG_C
Cuts at: 3073 3791 3838 4297 3073
Size: 718 47 459 5812
Fragments arranged by size:
5812 718 459 47
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7036
NotI GC'GGCC_GC
Cuts at: 4483 4483
Size: 7036
NspI r_CATG'y
Cuts at: 1132 1229 2357 3289 3352 3871 3913 4138 4394
Size: 97 1128 932 63 519 42 225 256
Cuts at: 4394 5939 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1128 932 519 256 225 97
63 42
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7036
PciI A'CATG_T
Cuts at: 3909 5935 3909
Size: 2026 5010
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3544 4024 4228 1108
Size: 2436 480 204 3916
Fragments arranged by size:
3916 2436 480 204
PfoI T'CCnGG_A
Cuts at: 2388 3132 2388
Size: 744 6292
PmeI GTTT'AAAC
Cuts at: 4340 4340
Size: 7036
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3173 3205 4013 4045 5105 5137 6618 6650 3173
Size: 32 808 32 1060 32 1481 32 3559
Fragments arranged by size:
3559 1481 1060 808 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2284 2284
Size: 7036
PshAI GACnn'nnGTC
Cuts at: 1613 2851 1613
Size: 1238 5798
PsiI TTA'TAA
Cuts at: 4606 4606
Size: 7036
PstI C_TGCA'G
Cuts at: 830 2513 2778 2796 3243 3417 3615 4388 830
Size: 1683 265 18 447 174 198 773 3478
Fragments arranged by size:
3478 1683 773 447 265 198 174 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7036
PvuII CAG'CTG
Cuts at: 2659 2746 2797 3361 3382 3532 3595 2659
Size: 87 51 564 21 150 63 6100
Fragments arranged by size:
6100 564 150 87 63 51 21
SacI G_AGCT'C
Cuts at: 2982 2982
Size: 7036
SalI G'TCGA_C
Cuts at: 1936 4378 1936
Size: 2442 4594
SapI GCTCTTCn'nnn_
Cuts at: 3465 3465
Size: 7036
SbfI CC_TGCA'GG
Cuts at: 2778 3615 4388 2778
Size: 837 773 5426
Fragments arranged by size:
5426 837 773
ScaI AGT'ACT
Cuts at: 2594 5284 6996 2594
Size: 2690 1712 2634
Fragments arranged by size:
2690 2634 1712
SfcI C'TryA_G
Cuts at: 826 1045 2509 2774 2792 3239 3413 3611 4384
Size: 219 1464 265 18 447 174 198 773
Cuts at: 4384 5519 6200 6391 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1464 1135 773 681 447 265 219
198 191 174 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 3950 4034 3950
Size: 84 6952
SfoI GGC'GCC
Cuts at: 2952 2952
Size: 7036
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7036
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7036
SmaI CCC'GGG
Cuts at: 1872 3446 4363 1872
Size: 1574 917 4545
Fragments arranged by size:
4545 1574 917
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5140 6041 6303 820
Size: 197 926 3197 901 262 1553
Fragments arranged by size:
3197 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4827 493
Size: 4334 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7036
SphI G_CATG'C
Cuts at: 1132 2357 3352 3871 4394 1132
Size: 1225 995 519 523 3774
Fragments arranged by size:
3774 1225 995 523 519
SspI AAT'ATT
Cuts at: 5 52 4960 5
Size: 47 4908 2081
Fragments arranged by size:
4908 2081 47
StuI AGG'CCT
Cuts at: 1517 2345 1517
Size: 828 6208
StyI C'CwwG_G
Cuts at: 513 1065 2004 2113 2268 2874 4083 4113 4475
Size: 552 939 109 155 606 1209 30 362
Cuts at: 4475 513
Size: 3074
Fragments arranged by size:
3074 1209 939 606 552 362 155 109
30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2473 5063 5080 5233 5418 5863 325
Size: 1255 893 2590 17 153 185 445 1498
Fragments arranged by size:
2590 1498 1255 893 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2376 2592 2925
Size: 274 80 33 51 1443 399 216 333
Cuts at: 2925 3399 3825 5282 6994 96
Size: 474 426 1457 1712 138
Fragments arranged by size:
1712 1457 1443 474 426 399 333 274
216 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4032 4064 6996 7028 4032
Size: 32 2932 32 4040
Fragments arranged by size:
4040 2932 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7036
XbaI T'CTAG_A
Cuts at: 4372 4372
Size: 7036
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2380 3115 4090 2380
Size: 735 975 5326
Fragments arranged by size:
5326 975 735
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7036
XmaI C'CCGG_G
Cuts at: 1870 3444 4361 1870
Size: 1574 917 4545
Fragments arranged by size:
4545 1574 917
XmnI GAAnn'nnTTC
Cuts at: 2581 5165 2581
Size: 2584 4452
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4844 276
Size: 53 83 186 936 3310 2468
Fragments arranged by size:
3310 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BfrBI BsiWI
BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SrfI SwaI