Restriction map for
pFN21AE1533
(Circular) (Six-base) MAPSORT of: pFN21AE1533.seq Check: 5847 from: 1 to: 7018
pFN21AE1533 7018 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4828 278
Size: 53 83 186 936 3292 2468
Fragments arranged by size:
3292 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4361 1937
Size: 2424 4594
Acc65I G'GTAC_C
Cuts at: 4339 4339
Size: 7018
AclI AA'CG_TT
Cuts at: 1563 5145 5518 1563
Size: 3582 373 3063
Fragments arranged by size:
3582 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2231 2259 2471 2669 4319 5081 6465 6733
Size: 599 28 212 198 1650 762 1384 268
Cuts at: 6733 1632
Size: 1917
Fragments arranged by size:
1917 1650 1384 762 599 268 212 198
28
AflII C'TTAA_G
Cuts at: 820 1017 2894 820
Size: 197 1877 4944
Fragments arranged by size:
4944 1877 197
AflIII A'CryG_T
Cuts at: 1203 2521 4280 5917 6894 1203
Size: 1318 1759 1637 977 1327
Fragments arranged by size:
1759 1637 1327 1318 977
AgeI A'CCGG_T
Cuts at: 4221 5849 4221
Size: 1628 5390
AhdI GACnn_n'nnGTC
Cuts at: 5747 5747
Size: 7018
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7018
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3123 1184
Size: 1939 5079
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3060 3092 3060
Size: 32 6986
AlwNI CAG_nnn'CTG
Cuts at: 2535 3141 3535 4761 6333 2535
Size: 606 394 1226 1572 3220
Fragments arranged by size:
3220 1572 1226 606 394
ApaLI G'TGCA_C
Cuts at: 5075 6231 5075
Size: 1156 5862
ApoI r'AATT_y
Cuts at: 1493 2565 3258 3272 3991 4327 4553 4705 6814
Size: 1072 693 14 719 336 226 152 2109
Cuts at: 6814 1493
Size: 1697
Fragments arranged by size:
2109 1697 1072 719 693 336 226 152
14
AseI AT'TA_AT
Cuts at: 160 1032 5572 160
Size: 872 4540 1606
Fragments arranged by size:
4540 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7018
AvaI C'yCGr_G
Cuts at: 1870 1943 2694 4343 1870
Size: 73 751 1649 4545
Fragments arranged by size:
4545 1649 751 73
BamHI G'GATC_C
Cuts at: 2385 4115 4348 4715 2385
Size: 1730 233 367 4688
Fragments arranged by size:
4688 1730 367 233
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4339 5794 618
Size: 325 217 627 2552 1455 1842
Fragments arranged by size:
2552 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2695 2701 3312 3501 4337 1391
Size: 1304 6 611 189 836 4072
Fragments arranged by size:
4072 1304 836 611 189 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2351 4215 4218 928
Size: 971 452 1864 3 3728
Fragments arranged by size:
3728 1864 971 452 3
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5209 5243 1903
Size: 34 3272 34 3678
Fragments arranged by size:
3678 3272 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4910 6120 4910
Size: 1210 5808
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7018
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5629 136
Size: 107 122 71 631 605 3957 1525
Fragments arranged by size:
3957 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2269 3302 7013 2269
Size: 1033 3711 2274
Fragments arranged by size:
3711 2274 1033
BlpI GC'TnA_GC
Cuts at: 3311 3324 4435 3311
Size: 13 1111 5894
Fragments arranged by size:
5894 1111 13
Bme1580I G_kGCm'C
Cuts at: 1790 5079 6235 1790
Size: 3289 1156 2573
Fragments arranged by size:
3289 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3874 4213 4283 1318
Size: 2556 339 70 4053
Fragments arranged by size:
4053 2556 339 70
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3921 4749 5707
Size: 416 53 83 399 24 2504 828 958
Cuts at: 5707 442
Size: 1753
Fragments arranged by size:
2504 1753 958 828 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7018
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2507 2576 2975 2999 5678 1239
Size: 1268 69 399 24 2679 2579
Fragments arranged by size:
2679 2579 1268 399 69 24
Bpu10I CC'TnA_GC
Cuts at: 3134 3183 3134
Size: 49 6969
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2107 2297 3221 4216 5143 6008 6306 2107
Size: 190 924 995 927 865 298 2819
Fragments arranged by size:
2819 995 927 924 865 298 190
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3508 5681 882
Size: 617 2009 2173 2219
Fragments arranged by size:
2219 2173 2009 617
BsaAI yAC'GTr
Cuts at: 493 1204 4809 493
Size: 711 3605 2702
Fragments arranged by size:
3605 2702 711
BsaBI GATnn'nnATC
Cuts at: 7012 7012
Size: 7018
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3943 4825 5207 5842
Size: 53 83 186 936 2410 882 382 635
Cuts at: 5842 275
Size: 1451
Fragments arranged by size:
2410 1451 936 882 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4221 5451 5849 6123 6270 6681 4221
Size: 1230 398 274 147 411 4558
Fragments arranged by size:
4558 1230 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2997 3027 3359 3389 3488
Size: 30 165 30 1594 30 332 30 99
Cuts at: 3488 3518 3678 3694 3708 3724 1178
Size: 30 160 16 14 16 4472
Fragments arranged by size:
4472 1594 332 165 160 99 30 30
30 30 30 16 16 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2510 2567 3056 3509 3761 3779 1186
Size: 1324 57 489 453 252 18 4425
Fragments arranged by size:
4425 1324 489 453 252 57 18
BseYI C'CCAG_C
Cuts at: 3553 3803 3894 6221 6717 3553
Size: 250 91 2327 496 3854
Fragments arranged by size:
3854 2327 496 250 91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3119 3127 1163
Size: 573 1383 8 5054
Fragments arranged by size:
5054 1383 573 8
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4468 5229 6257 1540
Size: 275 186 2467 761 1028 2301
Fragments arranged by size:
2467 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2529 3312 5079 5164 6235 2529
Size: 783 1767 85 1071 3312
Fragments arranged by size:
3312 1767 1071 783 85
BsmI GAATG_Cn'
Cuts at: 2402 3112 4529 4622 2402
Size: 710 1417 93 4798
Fragments arranged by size:
4798 1417 710 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2529 2695 2701 3312 3501 4337 5079
Size: 399 739 166 6 611 189 836 742
Cuts at: 5079 5164 6235 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 836 742 739 611 399 189
166 85 6
BspEI T'CCGG_A
Cuts at: 6681 6681
Size: 7018
BspHI T'CATG_A
Cuts at: 4907 6637 4907
Size: 1730 5288
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3487 4373 844
Size: 2643 886 3489
Fragments arranged by size:
3489 2643 886
BsrBI CCG'CTC
Cuts at: 1394 1823 4905 1394
Size: 429 3082 3507
Fragments arranged by size:
3507 3082 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2505 5513 5687 65
Size: 1183 1257 3008 174 1396
Fragments arranged by size:
3008 1396 1257 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2016 2457 3945 4221 5662 5849 1700
Size: 110 206 441 1488 276 1441 187 2869
Fragments arranged by size:
2869 1488 1441 441 276 206 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7018
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7018
BssSI C'ACGA_G
Cuts at: 5078 6090 5078
Size: 1012 6006
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7018
BstBI TT'CG_AA
Cuts at: 5822 5822
Size: 7018
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7018
BstXI CCAn_nnnn'nTGG
Cuts at: 2878 2909 2878
Size: 31 6987
BstYI r'GATC_y
Cuts at: 1972 2269 2385 3302 4115 4348 4715 5100 5117
Size: 297 116 917 813 233 367 385 17
Cuts at: 5117 6569 6655 6667 7013 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 917 813 385 367 346 297
233 116 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3651 3966 3651
Size: 315 6703
BtgI C'CryG_G
Cuts at: 513 1065 3208 513
Size: 552 2143 4323
Fragments arranged by size:
4323 2143 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6279
Fragments arranged by size:
6279 683 56
BtsI GCAGTG_nn'
Cuts at: 4534 5328 5348 4534
Size: 794 20 6204
Fragments arranged by size:
6204 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7018
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3855 3890 529
Size: 35 3291 35 3657
Fragments arranged by size:
3657 3291 35 35
DraI TTT'AAA
Cuts at: 4322 4322
Size: 7018
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2444 4247 6025 809
Size: 507 1128 1803 1778 1802
Fragments arranged by size:
1803 1802 1778 1128 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2304 2940 4465
Size: 54 1425 50 275 18 474 636 1525
Cuts at: 4465 5354 6983 8
Size: 889 1629 43
Fragments arranged by size:
1629 1525 1425 889 636 474 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4465 1537
Size: 275 2653 4090
Fragments arranged by size:
4090 2653 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2367 2652 2725 3033 3486 3534 3543 3756
Size: 1014 285 73 308 453 48 9 213
Cuts at: 3756 3833 4948 1353
Size: 77 1115 3423
Fragments arranged by size:
3423 1115 1014 453 308 285 213 77
73 48 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2194 5605 5979 6125 387
Size: 1064 138 605 3411 374 146 1280
Fragments arranged by size:
3411 1280 1064 605 374 146 138
EcoICRI GAG'CTC
Cuts at: 3310 3310
Size: 7018
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2259 2471 2507 2576 2669 2975
Size: 393 599 28 212 36 69 93 306
Cuts at: 2975 2999 4319 5081 5678 6465 6733 1239
Size: 24 1320 762 597 787 268 1524
Fragments arranged by size:
1524 1320 787 762 599 597 393 306
268 212 93 69 36 28 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2558 2657 2558
Size: 99 6919
EcoO109I rG'GnC_Cy
Cuts at: 2437 3178 3624 3971 2437
Size: 741 446 347 5484
Fragments arranged by size:
5484 741 446 347
EcoRI G'AATT_C
Cuts at: 4327 4705 4327
Size: 378 6640
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3089 3089
Size: 7018
FspI TGC'GCA
Cuts at: 5524 5524
Size: 7018
HaeII r_GCGC'y
Cuts at: 1678 6165 1678
Size: 4487 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2116 2148 3294 3326 3359
Size: 32 135 32 544 32 1146 32 33
Cuts at: 3359 3391 5659 5691 5733 5765 1373
Size: 32 2268 32 42 32 2626
Fragments arranged by size:
2626 2268 1146 544 135 42 33 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4362 4608 669
Size: 988 281 2424 246 3079
Fragments arranged by size:
3079 2424 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2138 3795 748
Size: 1390 1657 3971
Fragments arranged by size:
3971 1657 1390
HpaI GTT'AAC
Cuts at: 4608 4608
Size: 7018
KpnI G_GTAC'C
Cuts at: 4343 4343
Size: 7018
MfeI C'AATT_G
Cuts at: 4617 4617
Size: 7018
MluI A'CGCG_T
Cuts at: 6894 6894
Size: 7018
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4218 6132 6316 1305
Size: 2913 1914 184 2007
Fragments arranged by size:
2913 2007 1914 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2306 2942 10
Size: 54 1425 343 474 636 4086
Fragments arranged by size:
4086 1425 636 474 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4976 5335 5494 518
Size: 648 3810 359 159 2042
Fragments arranged by size:
3810 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2473 2815 2834 4037 4297 4434 5111 6259
Size: 867 342 19 1203 260 137 677 1148
Cuts at: 6259 6504 1606
Size: 245 2120
Fragments arranged by size:
2120 1203 1148 867 677 342 260 245
137 19
NcoI C'CATG_G
Cuts at: 513 1065 3208 513
Size: 552 2143 4323
Fragments arranged by size:
4323 2143 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7018
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7018
NotI GC'GGCC_GC
Cuts at: 4465 4465
Size: 7018
NspI r_CATG'y
Cuts at: 1132 1229 2525 4376 5921 1132
Size: 97 1296 1851 1545 2229
Fragments arranged by size:
2229 1851 1545 1296 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7018
PciI A'CATG_T
Cuts at: 2521 5917 2521
Size: 3396 3622
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7018
PfoI T'CCnGG_A
Cuts at: 3422 3422
Size: 7018
PmeI GTTT'AAAC
Cuts at: 4322 4322
Size: 7018
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2511 2543 5087 5119 6600 6632 2511
Size: 32 2544 32 1481 32 2897
Fragments arranged by size:
2897 2544 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2437 3178 3624 3971 2437
Size: 741 446 347 5484
Fragments arranged by size:
5484 741 446 347
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7018
PsiI TTA'TAA
Cuts at: 4588 4588
Size: 7018
PstI C_TGCA'G
Cuts at: 830 2706 2839 4370 830
Size: 1876 133 1531 3478
Fragments arranged by size:
3478 1876 1531 133
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7018
PvuII CAG'CTG
Cuts at: 2473 2815 2834 4037 4297 2473
Size: 342 19 1203 260 5194
Fragments arranged by size:
5194 1203 342 260 19
SacI G_AGCT'C
Cuts at: 3312 3312
Size: 7018
SalI G'TCGA_C
Cuts at: 1936 4360 1936
Size: 2424 4594
SanDI GG'GwC_CC
Cuts at: 2437 2437
Size: 7018
SapI GCTCTTCn'nnn_
Cuts at: 3534 3543 3534
Size: 9 7009
SbfI CC_TGCA'GG
Cuts at: 4370 4370
Size: 7018
ScaI AGT'ACT
Cuts at: 3390 5266 6978 3390
Size: 1876 1712 3430
Fragments arranged by size:
3430 1876 1712
SfcI C'TryA_G
Cuts at: 826 1045 2702 2835 3509 3686 4311 4366 5501
Size: 219 1657 133 674 177 625 55 1135
Cuts at: 5501 6182 6373 826
Size: 681 191 1471
Fragments arranged by size:
1657 1471 1135 681 674 625 219 191
177 133 55
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7018
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7018
SmaI CCC'GGG
Cuts at: 1872 4345 1872
Size: 2473 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2086 2276 2894 3200 4231 5122
Size: 197 926 143 190 618 306 1031 891
Cuts at: 5122 6023 6285 820
Size: 901 262 1553
Fragments arranged by size:
1553 1031 926 901 891 618 306 262
197 190 143
SnaBI TAC'GTA
Cuts at: 493 4809 493
Size: 4316 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7018
SphI G_CATG'C
Cuts at: 1132 4376 1132
Size: 3244 3774
SspI AAT'ATT
Cuts at: 5 52 4942 5
Size: 47 4890 2081
Fragments arranged by size:
4890 2081 47
StuI AGG'CCT
Cuts at: 1517 2633 1517
Size: 1116 5902
StyI C'CwwG_G
Cuts at: 513 1065 2721 3208 4161 4457 513
Size: 552 1656 487 953 296 3074
Fragments arranged by size:
3074 1656 953 552 487 296
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4145 5045 5062 5215 5400 5845 325
Size: 1255 2565 900 17 153 185 445 1498
Fragments arranged by size:
2565 1498 1255 900 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3388 3526 5264
Size: 274 80 33 51 1443 1411 138 1738
Cuts at: 5264 6976 96
Size: 1712 138
Fragments arranged by size:
1738 1712 1443 1411 274 138 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2997 3029 3694 3726 6978 7010 2997
Size: 32 665 32 3252 32 3005
Fragments arranged by size:
3252 3005 665 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2441 1316
Size: 1125 5893
XbaI T'CTAG_A
Cuts at: 4354 4354
Size: 7018
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2950 2950
Size: 7018
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7018
XmaI C'CCGG_G
Cuts at: 1870 4343 1870
Size: 2473 4545
XmnI GAAnn'nnTTC
Cuts at: 3570 5147 3570
Size: 1577 5441
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4826 276
Size: 53 83 186 936 3292 2468
Fragments arranged by size:
3292 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI
SfoI SrfI SwaI