Restriction map for
pFN21AE1531
(Circular) (Six-base) MAPSORT of: pFN21AE1531.seq Check: 7865 from: 1 to: 5905
pFN21AE1531 5905 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2448 2775 2907 3715
Size: 53 83 186 936 912 327 132 808
Cuts at: 3715 278
Size: 2468
Fragments arranged by size:
2468 936 912 808 327 186 132 83
53
AccI GT'mk_AC
Cuts at: 1937 3248 1937
Size: 1311 4594
Acc65I G'GTAC_C
Cuts at: 2357 3226 2357
Size: 869 5036
AclI AA'CG_TT
Cuts at: 1563 4032 4405 1563
Size: 2469 373 3063
Fragments arranged by size:
3063 2469 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2158 3968 5352 5620 1632
Size: 526 1810 1384 268 1917
Fragments arranged by size:
1917 1810 1384 526 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5708
AflIII A'CryG_T
Cuts at: 1203 4804 5781 1203
Size: 3601 977 1327
Fragments arranged by size:
3601 1327 977
AgeI A'CCGG_T
Cuts at: 4736 4736
Size: 5905
AhdI GACnn_n'nnGTC
Cuts at: 4634 4634
Size: 5905
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5905
AlwNI CAG_nnn'CTG
Cuts at: 2329 2740 3067 3648 5220 2329
Size: 411 327 581 1572 3014
Fragments arranged by size:
3014 1572 581 411 327
ApaLI G'TGCA_C
Cuts at: 2245 3962 5118 2245
Size: 1717 1156 3032
Fragments arranged by size:
3032 1717 1156
ApoI r'AATT_y
Cuts at: 1493 2140 2259 2341 2371 2945 3143 3214 3440
Size: 647 119 82 30 574 198 71 226
Cuts at: 3440 3592 5701 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 647 574 226 198 152 119
82 71 30
AseI AT'TA_AT
Cuts at: 160 1032 2817 4459 160
Size: 872 1785 1642 1606
Fragments arranged by size:
1785 1642 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
AvaI C'yCGr_G
Cuts at: 1870 1943 3230 1870
Size: 73 1287 4545
Fragments arranged by size:
4545 1287 73
BamHI G'GATC_C
Cuts at: 3235 3602 3235
Size: 367 5538
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2095 2357 3226 4681 618
Size: 325 217 627 308 262 869 1455 1842
Fragments arranged by size:
1842 1455 869 627 325 308 262 217
BanII G_rGCy'C
Cuts at: 1391 2868 2927 3224 1391
Size: 1477 59 297 4072
Fragments arranged by size:
4072 1477 297 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3129 928
Size: 971 1230 3704
Fragments arranged by size:
3704 1230 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3010 3044 4096 4130 1903
Size: 34 50 34 989 34 1052 34 3678
Fragments arranged by size:
3678 1052 989 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3051 3797 5007 3051
Size: 746 1210 3949
Fragments arranged by size:
3949 1210 746
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5905
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4516 136
Size: 107 122 71 631 605 2844 1525
Fragments arranged by size:
2844 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5900 5900
Size: 5905
BlpI GC'TnA_GC
Cuts at: 3322 3322
Size: 5905
Bme1580I G_kGCm'C
Cuts at: 1790 2249 3966 5122 1790
Size: 459 1717 1156 2573
Fragments arranged by size:
2573 1717 1156 459
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5905
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3636 4594 442
Size: 416 53 83 399 24 2219 958 1753
Fragments arranged by size:
2219 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2113 2950 4565 1239
Size: 874 837 1615 2579
Fragments arranged by size:
2579 1615 874 837
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4030 4895 5193 4030
Size: 865 298 4742
Fragments arranged by size:
4742 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4568 882
Size: 617 3069 2219
Fragments arranged by size:
3069 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2296 3696 493
Size: 711 1092 1400 2702
Fragments arranged by size:
2702 1400 1092 711
BsaBI GATnn'nnATC
Cuts at: 5899 5899
Size: 5905
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2445 2772 2904 3712
Size: 53 83 186 936 912 327 132 808
Cuts at: 3712 4094 4729 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 912 808 635 382 327 186
132 83 53
BsaWI w'CCGG_w
Cuts at: 2493 4338 4736 5010 5157 5568 2493
Size: 1845 398 274 147 411 2830
Fragments arranged by size:
2830 1845 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2480 2510 1178
Size: 30 165 30 1077 30 4573
Fragments arranged by size:
4573 1077 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5905
BseYI C'CCAG_C
Cuts at: 2050 2148 2666 2790 5108 5604 2050
Size: 98 518 124 2318 496 2351
Fragments arranged by size:
2351 2318 518 496 124 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5332
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3355 4116 5144 1540
Size: 275 186 1354 761 1028 2301
Fragments arranged by size:
2301 1354 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2249 2927 2994 3966 4051 5122 2249
Size: 678 67 972 85 1071 3032
Fragments arranged by size:
3032 1071 972 678 85 67
BsiWI C'GTAC_G
Cuts at: 2680 2707 2680
Size: 27 5878
BsmI GAATG_Cn'
Cuts at: 2054 3416 3509 2054
Size: 1362 93 4450
Fragments arranged by size:
4450 1362 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2571 2571
Size: 5905
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2249 2868 2927 2994 3224 3966 4051
Size: 399 459 619 59 67 230 742 85
Cuts at: 4051 5122 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 619 459 399 230 85
67 59
BspEI T'CCGG_A
Cuts at: 5568 5568
Size: 5905
BspHI T'CATG_A
Cuts at: 3794 5524 3794
Size: 1730 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3260 844
Size: 2416 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3792 1394
Size: 429 1969 3507
Fragments arranged by size:
3507 1969 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2677 4400 4574 65
Size: 1183 1429 1723 174 1396
Fragments arranged by size:
1723 1429 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4549 4736 1700
Size: 110 2739 187 2869
Fragments arranged by size:
2869 2739 187 110
BsrGI T'GTAC_A
Cuts at: 96 2460 2979 96
Size: 2364 519 3022
Fragments arranged by size:
3022 2364 519
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5905
BssSI C'ACGA_G
Cuts at: 3965 4977 3965
Size: 1012 4893
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5905
BstBI TT'CG_AA
Cuts at: 4709 4709
Size: 5905
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5905
BstYI r'GATC_y
Cuts at: 1972 2080 2490 3235 3602 3987 4004 5456 5542
Size: 108 410 745 367 385 17 1452 86
Cuts at: 5542 5554 5900 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 745 410 385 367 346 108
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3053 3053
Size: 5905
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5353
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5166
Fragments arranged by size:
5166 683 56
BtsI GCAGTG_nn'
Cuts at: 2698 3421 4215 4235 2698
Size: 723 794 20 4368
Fragments arranged by size:
4368 794 723 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5870
DraI TTT'AAA
Cuts at: 3043 3209 3043
Size: 166 5739
DraIII CAC_nnn'GTG
Cuts at: 2884 2884
Size: 5905
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4912 809
Size: 507 3596 1802
Fragments arranged by size:
3596 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3352 4241 5870
Size: 54 1425 50 275 18 1522 889 1629
Cuts at: 5870 8
Size: 43
Fragments arranged by size:
1629 1522 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3352 1537
Size: 275 1540 4090
Fragments arranged by size:
4090 1540 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3171 3835 1353
Size: 1818 664 3423
Fragments arranged by size:
3423 1818 664
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4492 4866 5012 387
Size: 1064 138 2903 374 146 1280
Fragments arranged by size:
2903 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2925 2925
Size: 5905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2113 2158 2950 3968 4565 5352 5620
Size: 393 481 45 792 1018 597 787 268
Cuts at: 5620 1239
Size: 1524
Fragments arranged by size:
1524 1018 792 787 597 481 393 268
45
EcoNI CCTnn'n_nnAGG
Cuts at: 2419 2419
Size: 5905
EcoO109I rG'GnC_Cy
Cuts at: 2727 2727
Size: 5905
EcoRI G'AATT_C
Cuts at: 3143 3214 3592 3143
Size: 71 378 5456
Fragments arranged by size:
5456 378 71
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 5905
FspI TGC'GCA
Cuts at: 4411 4411
Size: 5905
HaeII r_GCGC'y
Cuts at: 1678 5052 1678
Size: 3374 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2482 2514 2537 2569 4546
Size: 32 135 32 910 32 23 32 1977
Cuts at: 4546 4578 4620 4652 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1977 910 135 42 32 32 32
32 32 32 23
HincII GTy'rAC
Cuts at: 669 1657 1938 2587 3249 3495 669
Size: 988 281 649 662 246 3079
Fragments arranged by size:
3079 988 662 649 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5905
HpaI GTT'AAC
Cuts at: 2587 3495 2587
Size: 908 4997
KpnI G_GTAC'C
Cuts at: 2361 3230 2361
Size: 869 5036
MfeI C'AATT_G
Cuts at: 3504 3504
Size: 5905
MluI A'CGCG_T
Cuts at: 5781 5781
Size: 5905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2453 5019 5203 1305
Size: 1148 2566 184 2007
Fragments arranged by size:
2566 2007 1148 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4083
Fragments arranged by size:
4083 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2987 3863 4222 4381 518
Size: 648 1821 876 359 159 2042
Fragments arranged by size:
2042 1821 876 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2363 2767 3064 3321 3998 5146 5391 1606
Size: 757 404 297 257 677 1148 245 2120
Fragments arranged by size:
2120 1148 757 677 404 297 257 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5353
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5905
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5905
NotI GC'GGCC_GC
Cuts at: 3352 3352
Size: 5905
NspI r_CATG'y
Cuts at: 1132 1229 3263 4808 1132
Size: 97 2034 1545 2229
Fragments arranged by size:
2229 2034 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5905
PciI A'CATG_T
Cuts at: 4804 4804
Size: 5905
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3118 1108
Size: 2010 3895
PfoI T'CCnGG_A
Cuts at: 2129 2927 2129
Size: 798 5107
PmeI GTTT'AAAC
Cuts at: 3209 3209
Size: 5905
PmlI CAC'GTG
Cuts at: 2296 2296
Size: 5905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2208 2240 2957 2989 3974 4006 5487 5519 2208
Size: 32 717 32 985 32 1481 32 2594
Fragments arranged by size:
2594 1481 985 717 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2727 2727
Size: 5905
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5905
PsiI TTA'TAA
Cuts at: 3475 3475
Size: 5905
PstI C_TGCA'G
Cuts at: 830 2902 3257 830
Size: 2072 355 3478
Fragments arranged by size:
3478 2072 355
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5905
PvuII CAG'CTG
Cuts at: 2767 2767
Size: 5905
SacI G_AGCT'C
Cuts at: 2927 2927
Size: 5905
SalI G'TCGA_C
Cuts at: 1936 3247 1936
Size: 1311 4594
SbfI CC_TGCA'GG
Cuts at: 3257 3257
Size: 5905
ScaI AGT'ACT
Cuts at: 4153 5865 4153
Size: 1712 4193
SfcI C'TryA_G
Cuts at: 826 1045 2898 3253 4388 5069 5260 826
Size: 219 1853 355 1135 681 191 1471
Fragments arranged by size:
1853 1471 1135 681 355 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5905
SmaI CCC'GGG
Cuts at: 1872 3232 1872
Size: 1360 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4009 4910 5172 820
Size: 197 926 2066 901 262 1553
Fragments arranged by size:
2066 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3696 493
Size: 3203 2702
SpeI A'CTAG_T
Cuts at: 152 2303 152
Size: 2151 3754
SphI G_CATG'C
Cuts at: 1132 3263 1132
Size: 2131 3774
SspI AAT'ATT
Cuts at: 5 52 3829 5
Size: 47 3777 2081
Fragments arranged by size:
3777 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5905
StyI C'CwwG_G
Cuts at: 513 1065 3344 513
Size: 552 2279 3074
Fragments arranged by size:
3074 2279 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2064 3932 3949 4102 4287 4732 325
Size: 1255 484 1868 17 153 185 445 1498
Fragments arranged by size:
1868 1498 1255 484 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2460 2979 4151
Size: 274 80 33 51 1443 483 519 1172
Cuts at: 4151 5863 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1172 519 483 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2480 2512 2514 2546 5865 5897 2480
Size: 32 2 32 3319 32 2488
Fragments arranged by size:
3319 2488 32 32 32 2
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5905
XbaI T'CTAG_A
Cuts at: 3241 3241
Size: 5905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5905
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5905
XmaI C'CCGG_G
Cuts at: 1870 3230 1870
Size: 1360 4545
XmnI GAAnn'nnTTC
Cuts at: 4034 4034
Size: 5905
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2446 2773 2905 3713
Size: 53 83 186 936 912 327 132 808
Cuts at: 3713 276
Size: 2468
Fragments arranged by size:
2468 936 912 808 327 186 132 83
53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BstXI BstZ17I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI