Restriction map for
pFN21AE1522
(Circular) (Six-base) MAPSORT of: pFN21AE1522.seq Check: 2071 from: 1 to: 6481
pFN21AE1522 6481 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2807 2807
Size: 6481
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4291 278
Size: 53 83 186 936 2755 2468
Fragments arranged by size:
2755 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3189 3358 3648 3824 1937
Size: 1252 169 290 176 4594
Fragments arranged by size:
4594 1252 290 176 169
Acc65I G'GTAC_C
Cuts at: 3802 3802
Size: 6481
AclI AA'CG_TT
Cuts at: 1563 4608 4981 1563
Size: 3045 373 3063
Fragments arranged by size:
3063 3045 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3083 3621 4544 5928 6196 1632
Size: 1451 538 923 1384 268 1917
Fragments arranged by size:
1917 1451 1384 923 538 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6284
AflIII A'CryG_T
Cuts at: 1203 2194 2803 5380 6357 1203
Size: 991 609 2577 977 1327
Fragments arranged by size:
2577 1327 991 977 609
AgeI A'CCGG_T
Cuts at: 5312 5312
Size: 6481
AhdI GACnn_n'nnGTC
Cuts at: 5210 5210
Size: 6481
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6481
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6481
AlwNI CAG_nnn'CTG
Cuts at: 2789 2822 3242 4224 5796 2789
Size: 33 420 982 1572 3474
Fragments arranged by size:
3474 1572 982 420 33
ApaLI G'TGCA_C
Cuts at: 4538 5694 4538
Size: 1156 5325
ApoI r'AATT_y
Cuts at: 1493 2060 2832 3790 4016 4168 6277 1493
Size: 567 772 958 226 152 2109 1697
Fragments arranged by size:
2109 1697 958 772 567 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5035 160
Size: 872 4003 1606
Fragments arranged by size:
4003 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6481
AvaI C'yCGr_G
Cuts at: 1870 1943 2424 3806 1870
Size: 73 481 1382 4545
Fragments arranged by size:
4545 1382 481 73
BamHI G'GATC_C
Cuts at: 3811 4178 3811
Size: 367 6114
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3802 5257 618
Size: 325 217 627 2015 1455 1842
Fragments arranged by size:
2015 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2486 2707 3800 1391
Size: 1095 221 1093 4072
Fragments arranged by size:
4072 1095 1093 221
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2934 3146 928
Size: 971 1035 212 4263
Fragments arranged by size:
4263 1035 971 212
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4672 4706 1903
Size: 34 2735 34 3678
Fragments arranged by size:
3678 2735 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4373 5583 4373
Size: 1210 5271
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6481
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2534 5092 136
Size: 107 122 71 631 605 862 2558 1525
Fragments arranged by size:
2558 1525 862 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6476 6476
Size: 6481
BlpI GC'TnA_GC
Cuts at: 3898 3898
Size: 6481
Bme1580I G_kGCm'C
Cuts at: 1790 4542 5698 1790
Size: 2752 1156 2573
Fragments arranged by size:
2752 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2195 1318
Size: 877 5604
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3746 4212 5170
Size: 416 53 83 399 24 2329 466 958
Cuts at: 5170 442
Size: 1753
Fragments arranged by size:
2329 1753 958 466 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6481
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2325 5141 1239
Size: 1086 2816 2579
Fragments arranged by size:
2816 2579 1086
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2641 2747 4606 5471 5769 2641
Size: 106 1859 865 298 3353
Fragments arranged by size:
3353 1859 865 298 106
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3246 5144 882
Size: 617 1747 1898 2219
Fragments arranged by size:
2219 1898 1747 617
BsaAI yAC'GTr
Cuts at: 493 1204 4272 493
Size: 711 3068 2702
Fragments arranged by size:
3068 2702 711
BsaBI GATnn'nnATC
Cuts at: 2283 6475 2283
Size: 4192 2289
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2518 4288 4670 5305
Size: 53 83 186 936 985 1770 382 635
Cuts at: 5305 275
Size: 1451
Fragments arranged by size:
1770 1451 985 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2105 3692 4914 5312 5586 5733 6144 2105
Size: 1587 1222 398 274 147 411 2442
Fragments arranged by size:
2442 1587 1222 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6256
Fragments arranged by size:
6256 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2372 1186
Size: 1186 5295
BseYI C'CCAG_C
Cuts at: 2655 2812 5684 6180 2655
Size: 157 2872 496 2956
Fragments arranged by size:
2956 2872 496 157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3349 1163
Size: 573 1613 4295
Fragments arranged by size:
4295 1613 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3931 4692 5720 1540
Size: 275 186 1930 761 1028 2301
Fragments arranged by size:
2301 1930 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2486 4542 4627 5698 2486
Size: 2056 85 1071 3269
Fragments arranged by size:
3269 2056 1071 85
BsmI GAATG_Cn'
Cuts at: 2537 3992 4085 2537
Size: 1455 93 4933
Fragments arranged by size:
4933 1455 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2486 2707 3800 4542 4627 5698 1391
Size: 399 696 221 1093 742 85 1071 2174
Fragments arranged by size:
2174 1093 1071 742 696 399 221 85
BspEI T'CCGG_A
Cuts at: 2105 6144 2105
Size: 4039 2442
BspHI T'CATG_A
Cuts at: 4370 6100 4370
Size: 1730 4751
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2807 3313 3836 844
Size: 1963 506 523 3489
Fragments arranged by size:
3489 1963 523 506
BsrBI CCG'CTC
Cuts at: 1394 1823 2081 4368 1394
Size: 429 258 2287 3507
Fragments arranged by size:
3507 2287 429 258
BsrDI GCAATG_nn'
Cuts at: 65 1248 3214 3270 3678 4976 5150 65
Size: 1183 1966 56 408 1298 174 1396
Fragments arranged by size:
1966 1396 1298 1183 408 174 56
BsrFI r'CCGG_y
Cuts at: 1700 1810 2048 5125 5312 1700
Size: 110 238 3077 187 2869
Fragments arranged by size:
3077 2869 238 187 110
BsrGI T'GTAC_A
Cuts at: 96 2661 96
Size: 2565 3916
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6481
BssSI C'ACGA_G
Cuts at: 4541 5553 4541
Size: 1012 5469
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2222 2822 794
Size: 1428 600 4453
Fragments arranged by size:
4453 1428 600
BstBI TT'CG_AA
Cuts at: 3139 5285 3139
Size: 2146 4335
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6481
BstYI r'GATC_y
Cuts at: 1972 3811 4178 4563 4580 6032 6118 6130 6476
Size: 1839 367 385 17 1452 86 12 346
Cuts at: 6476 1972
Size: 1977
Fragments arranged by size:
1977 1839 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3359 3359
Size: 6481
BtgI C'CryG_G
Cuts at: 513 1065 2004 2469 513
Size: 552 939 465 4525
Fragments arranged by size:
4525 939 552 465
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2742 487
Size: 683 56 1516 4226
Fragments arranged by size:
4226 1516 683 56
BtsI GCAGTG_nn'
Cuts at: 2666 3361 3997 4791 4811 2666
Size: 695 636 794 20 4336
Fragments arranged by size:
4336 794 695 636 20
ClaI AT'CG_AT
Cuts at: 1554 3477 1554
Size: 1923 4558
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6446
DraI TTT'AAA
Cuts at: 3439 3785 3439
Size: 346 6135
DraIII CAC_nnn'GTG
Cuts at: 2843 2843
Size: 6481
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5488 809
Size: 507 4172 1802
Fragments arranged by size:
4172 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2014 2268 3928
Size: 54 1425 50 275 18 184 254 1660
Cuts at: 3928 4817 6446 8
Size: 889 1629 43
Fragments arranged by size:
1660 1629 1425 889 275 254 184 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3928 1537
Size: 275 2116 4090
Fragments arranged by size:
4090 2116 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3164 3446 3776 4411 1353
Size: 1811 282 330 635 3423
Fragments arranged by size:
3423 1811 635 330 282
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5068 5442 5588 387
Size: 1064 138 3479 374 146 1280
Fragments arranged by size:
3479 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2484 2484
Size: 6481
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2325 3083 3621 4544 5141 5928 6196
Size: 393 693 758 538 923 597 787 268
Cuts at: 6196 1239
Size: 1524
Fragments arranged by size:
1524 923 787 758 693 597 538 393
268
EcoRI G'AATT_C
Cuts at: 2832 3790 4168 2832
Size: 958 378 5145
Fragments arranged by size:
5145 958 378
FspI TGC'GCA
Cuts at: 4987 4987
Size: 6481
HaeII r_GCGC'y
Cuts at: 1678 5628 1678
Size: 3950 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2924 2956 3243 3275 3447
Size: 32 135 32 1352 32 287 32 172
Cuts at: 3447 3479 5122 5154 5196 5228 1373
Size: 32 1643 32 42 32 2626
Fragments arranged by size:
2626 1643 1352 287 172 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2166 3190 3825 4071 669
Size: 988 281 228 1024 635 246 3079
Fragments arranged by size:
3079 1024 988 635 281 246 228
HindIII A'AGCT_T
Cuts at: 748 2678 748
Size: 1930 4551
HpaI GTT'AAC
Cuts at: 4071 4071
Size: 6481
KpnI G_GTAC'C
Cuts at: 3806 3806
Size: 6481
MfeI C'AATT_G
Cuts at: 4080 4080
Size: 6481
MluI A'CGCG_T
Cuts at: 6357 6357
Size: 6481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2809 5595 5779 1305
Size: 1504 2786 184 2007
Fragments arranged by size:
2786 2007 1504 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2270 10
Size: 54 1425 343 438 4221
Fragments arranged by size:
4221 1425 438 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2474 4439 4798 4957 518
Size: 648 1308 1965 359 159 2042
Fragments arranged by size:
2042 1965 1308 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2659 2749 2789 2906 3897 4574 5722 5967
Size: 1053 90 40 117 991 677 1148 245
Cuts at: 5967 1606
Size: 2120
Fragments arranged by size:
2120 1148 1053 991 677 245 117 90
40
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4990
Fragments arranged by size:
4990 939 552
NdeI CA'TA_TG
Cuts at: 387 2911 387
Size: 2524 3957
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6481
NotI GC'GGCC_GC
Cuts at: 3928 3928
Size: 6481
NspI r_CATG'y
Cuts at: 1132 1229 2807 3839 5384 1132
Size: 97 1578 1032 1545 2229
Fragments arranged by size:
2229 1578 1545 1032 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6481
PciI A'CATG_T
Cuts at: 2803 5380 2803
Size: 2577 3904
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6481
PmeI GTTT'AAAC
Cuts at: 3785 3785
Size: 6481
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4550 4582 6063 6095 4550
Size: 32 1481 32 4936
Fragments arranged by size:
4936 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3349 1613
Size: 1736 4745
PsiI TTA'TAA
Cuts at: 2291 2717 4051 2291
Size: 426 1334 4721
Fragments arranged by size:
4721 1334 426
PspXI vC'TCGA_Gb
Cuts at: 2424 2424
Size: 6481
PstI C_TGCA'G
Cuts at: 830 2218 2410 2673 3833 830
Size: 1388 192 263 1160 3478
Fragments arranged by size:
3478 1388 1160 263 192
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6481
PvuII CAG'CTG
Cuts at: 2659 2749 2659
Size: 90 6391
SacI G_AGCT'C
Cuts at: 2486 2486
Size: 6481
SalI G'TCGA_C
Cuts at: 1936 3188 3823 1936
Size: 1252 635 4594
Fragments arranged by size:
4594 1252 635
SapI GCTCTTCn'nnn_
Cuts at: 3776 3776
Size: 6481
SbfI CC_TGCA'GG
Cuts at: 3833 3833
Size: 6481
ScaI AGT'ACT
Cuts at: 4729 6441 4729
Size: 1712 4769
SfcI C'TryA_G
Cuts at: 826 1045 2214 2406 2669 3829 4964 5645 5836
Size: 219 1169 192 263 1160 1135 681 191
Cuts at: 5836 826
Size: 1471
Fragments arranged by size:
1471 1169 1160 1135 681 263 219 192
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6481
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6481
SmaI CCC'GGG
Cuts at: 1872 3808 1872
Size: 1936 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2424 2620 2762 4585 5486 5748
Size: 197 926 481 196 142 1823 901 262
Cuts at: 5748 820
Size: 1553
Fragments arranged by size:
1823 1553 926 901 481 262 197 196
142
SnaBI TAC'GTA
Cuts at: 493 4272 493
Size: 3779 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6481
SphI G_CATG'C
Cuts at: 1132 3839 1132
Size: 2707 3774
SspI AAT'ATT
Cuts at: 5 52 2245 4405 5
Size: 47 2193 2160 2081
Fragments arranged by size:
2193 2160 2081 47
StuI AGG'CCT
Cuts at: 1517 3725 1517
Size: 2208 4273
StyI C'CwwG_G
Cuts at: 513 1065 2004 2505 3920 513
Size: 552 939 501 1415 3074
Fragments arranged by size:
3074 1415 939 552 501
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2911 4508 4525 4678 4863 5308 325
Size: 1255 1331 1597 17 153 185 445 1498
Fragments arranged by size:
1597 1498 1331 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2592 2661 3282
Size: 274 80 33 51 1443 615 69 621
Cuts at: 3282 4727 6439 96
Size: 1445 1712 138
Fragments arranged by size:
1712 1445 1443 621 615 274 138 80
69 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6441 6473 6441
Size: 32 6449
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6481
XbaI T'CTAG_A
Cuts at: 3817 3817
Size: 6481
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2863 2863
Size: 6481
XhoI C'TCGA_G
Cuts at: 1943 2424 1943
Size: 481 6000
XmaI C'CCGG_G
Cuts at: 1870 3806 1870
Size: 1936 4545
XmnI GAAnn'nnTTC
Cuts at: 2537 4610 2537
Size: 2073 4408
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4289 276
Size: 53 83 186 936 2755 2468
Fragments arranged by size:
2755 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI