Restriction map for
pFN21AE1519
(Circular) (Six-base) MAPSORT of: pFN21AE1519.seq Check: 6691 from: 1 to: 6097
pFN21AE1519 6097 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3907 278
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3440 1937
Size: 1503 4594
Acc65I G'GTAC_C
Cuts at: 3418 3418
Size: 6097
AclI AA'CG_TT
Cuts at: 1563 3396 4224 4597 1563
Size: 1833 828 373 3063
Fragments arranged by size:
3063 1833 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4160 5544 5812 1632
Size: 2528 1384 268 1917
Fragments arranged by size:
2528 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5900
AflIII A'CryG_T
Cuts at: 1203 4996 5973 1203
Size: 3793 977 1327
Fragments arranged by size:
3793 1327 977
AgeI A'CCGG_T
Cuts at: 4928 4928
Size: 6097
AhdI GACnn_n'nnGTC
Cuts at: 4826 4826
Size: 6097
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6097
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2910 1184
Size: 1726 4371
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2570 2602 2570
Size: 32 6065
AlwNI CAG_nnn'CTG
Cuts at: 2509 2810 2921 2942 2963 3005 3068 3840 5412
Size: 301 111 21 21 42 63 772 1572
Cuts at: 5412 2509
Size: 3194
Fragments arranged by size:
3194 1572 772 301 111 63 42 21
21
ApaI G_GGCC'C
Cuts at: 2826 2826
Size: 6097
ApaLI G'TGCA_C
Cuts at: 3140 3182 3308 4154 5310 3140
Size: 42 126 846 1156 3927
Fragments arranged by size:
3927 1156 846 126 42
ApoI r'AATT_y
Cuts at: 1493 3406 3632 3784 5893 1493
Size: 1913 226 152 2109 1697
Fragments arranged by size:
2109 1913 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4651 160
Size: 872 3619 1606
Fragments arranged by size:
3619 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
AvaI C'yCGr_G
Cuts at: 1870 1943 2024 2347 2827 3026 3133 3217 3239
Size: 73 81 323 480 199 107 84 22
Cuts at: 3239 3422 1870
Size: 183 4545
Fragments arranged by size:
4545 480 323 199 183 107 84 81
73 22
BamHI G'GATC_C
Cuts at: 3427 3794 3427
Size: 367 5730
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2551 3418 4873 618
Size: 325 217 627 764 867 1455 1842
Fragments arranged by size:
1842 1455 867 764 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2162 2529 2826 3416 1391
Size: 771 367 297 590 4072
Fragments arranged by size:
4072 771 590 367 297
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5126
BbvCI CC'TCA_GC
Cuts at: 3273 3338 3273
Size: 65 6032
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2522 2548 2556 2582 4288 4322 1903
Size: 34 585 26 8 26 1706 34 3678
Fragments arranged by size:
3678 1706 585 34 34 26 26 8
BciVI GTATCCnnnnn_n'
Cuts at: 3989 5199 3989
Size: 1210 4887
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6097
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2028 2270 2279
Size: 107 122 71 631 605 356 242 9
Cuts at: 2279 2349 2734 2821 4708 136
Size: 70 385 87 1887 1525
Fragments arranged by size:
1887 1525 631 605 385 356 242 122
107 87 71 70 9
BglII A'GATC_T
Cuts at: 6092 6092
Size: 6097
BlpI GC'TnA_GC
Cuts at: 3514 3514
Size: 6097
Bme1580I G_kGCm'C
Cuts at: 1790 2826 3144 3186 3312 4158 5314 1790
Size: 1036 318 42 126 846 1156 2573
Fragments arranged by size:
2573 1156 1036 846 318 126 42
BmgBI CAC'GTC
Cuts at: 1318 2447 1318
Size: 1129 4968
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2873 3828 4786
Size: 416 53 83 399 24 1456 955 958
Cuts at: 4786 442
Size: 1753
Fragments arranged by size:
1753 1456 958 955 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 2612 2841 3003 3027 4757 1239
Size: 890 483 229 162 24 1730 2579
Fragments arranged by size:
2579 1730 890 483 229 162 24
Bpu10I CC'TnA_GC
Cuts at: 3273 3338 3273
Size: 65 6032
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4222 5087 5385 4222
Size: 865 298 4934
Fragments arranged by size:
4934 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2105 2528 2603 4760 882
Size: 617 606 423 75 2157 2219
Fragments arranged by size:
2219 2157 617 606 423 75
BsaAI yAC'GTr
Cuts at: 493 1204 3888 493
Size: 711 2684 2702
Fragments arranged by size:
2702 2684 711
BsaBI GATnn'nnATC
Cuts at: 6091 6091
Size: 6097
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2925 2946 3904 4286
Size: 53 83 186 936 1392 21 958 382
Cuts at: 4286 4921 275
Size: 635 1451
Fragments arranged by size:
1451 1392 958 936 635 382 186 83
53 21
BsaWI w'CCGG_w
Cuts at: 2488 4530 4928 5202 5349 5760 2488
Size: 2042 398 274 147 411 2825
Fragments arranged by size:
2825 2042 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2144 2174 2570 2600 2655
Size: 30 165 30 741 30 396 30 55
Cuts at: 2655 2685 1178
Size: 30 4590
Fragments arranged by size:
4590 741 396 165 55 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2047 2050 2390 1186
Size: 861 3 340 4893
Fragments arranged by size:
4893 861 340 3
BseYI C'CCAG_C
Cuts at: 2289 2666 2838 2914 2935 2956 2998 3145 5300
Size: 377 172 76 21 21 42 147 2155
Cuts at: 5300 5796 2289
Size: 496 2590
Fragments arranged by size:
2590 2155 496 377 172 147 76 42
21 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5524
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2344 3547 4308 5336 1540
Size: 275 186 343 1203 761 1028 2301
Fragments arranged by size:
2301 1203 1028 761 343 275 186
BsiHKAI G_wGCw'C
Cuts at: 3144 3186 3312 4158 4243 5314 3144
Size: 42 126 846 85 1071 3927
Fragments arranged by size:
3927 1071 846 126 85 42
BsiWI C'GTAC_G
Cuts at: 2466 2466
Size: 6097
BsmI GAATG_Cn'
Cuts at: 2174 3608 3701 2174
Size: 1434 93 4570
Fragments arranged by size:
4570 1434 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2162 2529 2826 3144 3186 3312 3416
Size: 399 372 367 297 318 42 126 104
Cuts at: 3416 4158 4243 5314 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 372 367 318 297
126 104 85 42
BspEI T'CCGG_A
Cuts at: 2488 5760 2488
Size: 3272 2825
BspHI T'CATG_A
Cuts at: 2142 3986 5716 2142
Size: 1844 1730 2523
Fragments arranged by size:
2523 1844 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2517 3452 844
Size: 1673 935 3489
Fragments arranged by size:
3489 1673 935
BsrBI CCG'CTC
Cuts at: 1394 1823 2043 3984 1394
Size: 429 220 1941 3507
Fragments arranged by size:
3507 1941 429 220
BsrDI GCAATG_nn'
Cuts at: 65 1248 2538 4592 4766 65
Size: 1183 1290 2054 174 1396
Fragments arranged by size:
2054 1396 1290 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2493 4741 4928 1700
Size: 110 683 2248 187 2869
Fragments arranged by size:
2869 2248 683 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6097
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6097
BssSI C'ACGA_G
Cuts at: 4157 5169 4157
Size: 1012 5085
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2178 2509 794
Size: 1384 331 4382
Fragments arranged by size:
4382 1384 331
BstBI TT'CG_AA
Cuts at: 4901 4901
Size: 6097
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6097
BstYI r'GATC_y
Cuts at: 1972 3427 3794 4179 4196 5648 5734 5746 6092
Size: 1455 367 385 17 1452 86 12 346
Cuts at: 6092 1972
Size: 1977
Fragments arranged by size:
1977 1455 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2818 3168 3210 2818
Size: 350 42 5705
Fragments arranged by size:
5705 350 42
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4606
Fragments arranged by size:
4606 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2003 487
Size: 683 56 777 4581
Fragments arranged by size:
4581 777 683 56
BtsI GCAGTG_nn'
Cuts at: 3613 4407 4427 3613
Size: 794 20 5283
Fragments arranged by size:
5283 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6097
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6062
DraI TTT'AAA
Cuts at: 3401 3401
Size: 6097
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5104 809
Size: 507 3788 1802
Fragments arranged by size:
3788 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2341 2517 2735
Size: 54 1425 50 275 18 511 176 218
Cuts at: 2735 3544 4433 6062 8
Size: 809 889 1629 43
Fragments arranged by size:
1629 1425 889 809 511 275 218 176
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2341 3544 1537
Size: 275 529 1203 4090
Fragments arranged by size:
4090 1203 529 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2408 4027 1353
Size: 1055 1619 3423
Fragments arranged by size:
3423 1619 1055
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2591 4684 5058 5204 387
Size: 1064 138 1002 2093 374 146 1280
Fragments arranged by size:
2093 1280 1064 1002 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2612 2841 3003 3027 4160 4757
Size: 393 497 483 229 162 24 1133 597
Cuts at: 4757 5544 5812 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1133 787 597 497 483 393 268
229 162 24
EcoO109I rG'GnC_Cy
Cuts at: 2131 2281 2807 2822 2823 3130 3172 3214 3342
Size: 150 526 15 1 307 42 42 128
Cuts at: 3342 2131
Size: 4886
Fragments arranged by size:
4886 526 307 150 128 42 42 15
1
EcoRI G'AATT_C
Cuts at: 3406 3784 3406
Size: 378 5719
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2638 2638
Size: 6097
FspI TGC'GCA
Cuts at: 4603 4603
Size: 6097
HaeII r_GCGC'y
Cuts at: 1678 2092 2500 5244 1678
Size: 414 408 2744 2531
Fragments arranged by size:
2744 2531 414 408
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2470 2502 2707 2739 3193
Size: 32 135 32 898 32 205 32 454
Cuts at: 3193 3225 4738 4770 4812 4844 1373
Size: 32 1513 32 42 32 2626
Fragments arranged by size:
2626 1513 898 454 205 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3441 3687 669
Size: 988 281 1503 246 3079
Fragments arranged by size:
3079 1503 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6097
HpaI GTT'AAC
Cuts at: 3687 3687
Size: 6097
KpnI G_GTAC'C
Cuts at: 3422 3422
Size: 6097
MfeI C'AATT_G
Cuts at: 3696 3696
Size: 6097
MluI A'CGCG_T
Cuts at: 5973 5973
Size: 6097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5211 5395 1305
Size: 3906 184 2007
Fragments arranged by size:
3906 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4275
Fragments arranged by size:
4275 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2196 4055 4414 4573 518
Size: 648 1030 1859 359 159 2042
Fragments arranged by size:
2042 1859 1030 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2101 2713 2743 3113 3513 4190 5338 5583
Size: 495 612 30 370 400 677 1148 245
Cuts at: 5583 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 612 495 400 370 245
30
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4606
Fragments arranged by size:
4606 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6097
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6097
NotI GC'GGCC_GC
Cuts at: 3544 3544
Size: 6097
NspI r_CATG'y
Cuts at: 1132 1229 3455 5000 1132
Size: 97 2226 1545 2229
Fragments arranged by size:
2229 2226 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2687 2963 3068 3089 3176 3197 3365 1348
Size: 1339 276 105 21 87 21 168 4080
Fragments arranged by size:
4080 1339 276 168 105 87 21 21
PciI A'CATG_T
Cuts at: 4996 4996
Size: 6097
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2921 2942 2963 2984 3005 3068 1108
Size: 1813 21 21 21 21 63 4137
Fragments arranged by size:
4137 1813 63 21 21 21 21
PfoI T'CCnGG_A
Cuts at: 3004 3280 3004
Size: 276 5821
PmeI GTTT'AAAC
Cuts at: 3401 3401
Size: 6097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2570 2602 4166 4198 5679 5711 2570
Size: 32 1564 32 1481 32 2956
Fragments arranged by size:
2956 1564 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2131 2131
Size: 6097
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6097
PsiI TTA'TAA
Cuts at: 3667 3667
Size: 6097
PspOMI G'GGCC_C
Cuts at: 2822 2822
Size: 6097
PstI C_TGCA'G
Cuts at: 830 3449 830
Size: 2619 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6097
PvuII CAG'CTG
Cuts at: 2101 2101
Size: 6097
RsrII CG'GwC_CG
Cuts at: 2491 2491
Size: 6097
SalI G'TCGA_C
Cuts at: 1936 3439 1936
Size: 1503 4594
SbfI CC_TGCA'GG
Cuts at: 3449 3449
Size: 6097
ScaI AGT'ACT
Cuts at: 4345 6057 4345
Size: 1712 4385
SfcI C'TryA_G
Cuts at: 826 1045 3445 4580 5261 5452 826
Size: 219 2400 1135 681 191 1471
Fragments arranged by size:
2400 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2279 2279
Size: 6097
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6097
SmaI CCC'GGG
Cuts at: 1872 2026 2349 2829 3028 3135 3219 3241 3424
Size: 154 323 480 199 107 84 22 183
Cuts at: 3424 1872
Size: 4545
Fragments arranged by size:
4545 480 323 199 183 154 107 84
22
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4201 5102 5364 820
Size: 197 926 2258 901 262 1553
Fragments arranged by size:
2258 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3888 493
Size: 3395 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6097
SphI G_CATG'C
Cuts at: 1132 3455 1132
Size: 2323 3774
SrfI GCCC'GGGC
Cuts at: 2349 2349
Size: 6097
SspI AAT'ATT
Cuts at: 5 52 4021 5
Size: 47 3969 2081
Fragments arranged by size:
3969 2081 47
StuI AGG'CCT
Cuts at: 1517 2778 1517
Size: 1261 4836
StyI C'CwwG_G
Cuts at: 513 1065 2004 2265 2274 3536 513
Size: 552 939 261 9 1262 3074
Fragments arranged by size:
3074 1262 939 552 261 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2680 2806 2988 3051 3072 3093 3159
Size: 1255 1100 126 182 63 21 21 66
Cuts at: 3159 3201 4124 4141 4294 4479 4924 325
Size: 42 923 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1100 923 445 185 182 153
126 66 63 42 21 21 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4343 6055 96
Size: 274 80 33 51 1443 2366 1712 138
Fragments arranged by size:
2366 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2144 2176 2655 2687 6057 6089 2144
Size: 32 479 32 3370 32 2152
Fragments arranged by size:
3370 2152 479 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6097
XbaI T'CTAG_A
Cuts at: 3433 3433
Size: 6097
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6097
XmaI C'CCGG_G
Cuts at: 1870 2024 2347 2827 3026 3133 3217 3239 3422
Size: 154 323 480 199 107 84 22 183
Cuts at: 3422 1870
Size: 4545
Fragments arranged by size:
4545 480 323 199 183 154 107 84
22
XmnI GAAnn'nnTTC
Cuts at: 4226 4226
Size: 6097
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3905 276
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI SacI SacII SanDI SapI SexAI
SfoI SwaI XcmI