Restriction map for
pFN21AE1511
(Circular) (Six-base) MAPSORT of: pFN21AE1511.seq Check: 4129 from: 1 to: 5671
pFN21AE1511 5671 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3481 278
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3014 1937
Size: 1077 4594
Acc65I G'GTAC_C
Cuts at: 2992 2992
Size: 5671
AclI AA'CG_TT
Cuts at: 1563 3798 4171 1563
Size: 2235 373 3063
Fragments arranged by size:
3063 2235 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2471 2628 3734 5118 5386 1632
Size: 839 157 1106 1384 268 1917
Fragments arranged by size:
1917 1384 1106 839 268 157
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5474
AflIII A'CryG_T
Cuts at: 1203 4570 5547 1203
Size: 3367 977 1327
Fragments arranged by size:
3367 1327 977
AgeI A'CCGG_T
Cuts at: 4502 4502
Size: 5671
AhdI GACnn_n'nnGTC
Cuts at: 4400 4400
Size: 5671
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5671
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5671
AlwNI CAG_nnn'CTG
Cuts at: 2735 3414 4986 2735
Size: 679 1572 3420
Fragments arranged by size:
3420 1572 679
ApaLI G'TGCA_C
Cuts at: 3728 4884 3728
Size: 1156 4515
ApoI r'AATT_y
Cuts at: 1493 2980 3206 3358 5467 1493
Size: 1487 226 152 2109 1697
Fragments arranged by size:
2109 1697 1487 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4225 160
Size: 872 3193 1606
Fragments arranged by size:
3193 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
AvaI C'yCGr_G
Cuts at: 1870 1943 2925 2996 1870
Size: 73 982 71 4545
Fragments arranged by size:
4545 982 73 71
BamHI G'GATC_C
Cuts at: 3001 3368 3001
Size: 367 5304
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2992 4447 618
Size: 325 217 627 1205 1455 1842
Fragments arranged by size:
1842 1455 1205 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2044 2990 1391
Size: 653 946 4072
Fragments arranged by size:
4072 946 653
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2861 928
Size: 971 962 3738
Fragments arranged by size:
3738 971 962
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2728 2762 3862 3896 1903
Size: 34 791 34 1100 34 3678
Fragments arranged by size:
3678 1100 791 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3563 4773 3563
Size: 1210 4461
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5671
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4282 136
Size: 107 122 71 631 605 2610 1525
Fragments arranged by size:
2610 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5666 5666
Size: 5671
BlpI GC'TnA_GC
Cuts at: 3088 3088
Size: 5671
Bme1580I G_kGCm'C
Cuts at: 1790 3732 4888 1790
Size: 1942 1156 2573
Fragments arranged by size:
2573 1942 1156
BmgBI CAC'GTC
Cuts at: 1318 2548 1318
Size: 1230 4441
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3402 4360 442
Size: 416 53 83 399 24 1985 958 1753
Fragments arranged by size:
1985 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5671
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2792 2792
Size: 5671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4331 1239
Size: 3092 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2135 2560 3796 4661 4959 2135
Size: 425 1236 865 298 2847
Fragments arranged by size:
2847 1236 865 425 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2295 2780 4334 882
Size: 617 796 485 1554 2219
Fragments arranged by size:
2219 1554 796 617 485
BsaAI yAC'GTr
Cuts at: 493 1204 3462 493
Size: 711 2258 2702
Fragments arranged by size:
2702 2258 711
BsaBI GATnn'nnATC
Cuts at: 5665 5665
Size: 5671
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3478 3860 4495 275
Size: 53 83 186 936 1945 382 635 1451
Fragments arranged by size:
1945 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4104 4502 4776 4923 5334 4104
Size: 398 274 147 411 4441
Fragments arranged by size:
4441 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5446
Fragments arranged by size:
5446 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2082 1186
Size: 896 4775
BseYI C'CCAG_C
Cuts at: 2293 4874 5370 2293
Size: 2581 496 2594
Fragments arranged by size:
2594 2581 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5098
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3121 3882 4910 1540
Size: 275 186 1120 761 1028 2301
Fragments arranged by size:
2301 1120 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2044 3732 3817 4888 2044
Size: 1688 85 1071 2827
Fragments arranged by size:
2827 1688 1071 85
BsmI GAATG_Cn'
Cuts at: 3182 3275 3182
Size: 93 5578
BsmBI CGTCTCn'nnnn_
Cuts at: 2538 2538
Size: 5671
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2044 2990 3732 3817 4888 1391
Size: 399 254 946 742 85 1071 2174
Fragments arranged by size:
2174 1071 946 742 399 254 85
BspEI T'CCGG_A
Cuts at: 5334 5334
Size: 5671
BspHI T'CATG_A
Cuts at: 3560 5290 3560
Size: 1730 3941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2169 3026 844
Size: 1325 857 3489
Fragments arranged by size:
3489 1325 857
BsrBI CCG'CTC
Cuts at: 1394 1823 3558 1394
Size: 429 1735 3507
Fragments arranged by size:
3507 1735 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4166 4340 65
Size: 1183 2918 174 1396
Fragments arranged by size:
2918 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4315 4502 1700
Size: 110 2505 187 2869
Fragments arranged by size:
2869 2505 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5671
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5671
BssSI C'ACGA_G
Cuts at: 2064 3731 4743 2064
Size: 1667 1012 2992
Fragments arranged by size:
2992 1667 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5671
BstBI TT'CG_AA
Cuts at: 4475 4475
Size: 5671
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5671
BstYI r'GATC_y
Cuts at: 1972 3001 3368 3753 3770 5222 5308 5320 5666
Size: 1029 367 385 17 1452 86 12 346
Cuts at: 5666 1972
Size: 1977
Fragments arranged by size:
1977 1452 1029 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2967 2967
Size: 5671
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5119
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4932
Fragments arranged by size:
4932 683 56
BtsI GCAGTG_nn'
Cuts at: 3187 3981 4001 3187
Size: 794 20 4857
Fragments arranged by size:
4857 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2202 2237 529
Size: 35 1638 35 3963
Fragments arranged by size:
3963 1638 35 35
DraI TTT'AAA
Cuts at: 2975 2975
Size: 5671
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4678 809
Size: 507 3362 1802
Fragments arranged by size:
3362 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2332 3118 4007
Size: 54 1425 50 275 18 502 786 889
Cuts at: 4007 5636 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 786 502 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3118 1537
Size: 275 1306 4090
Fragments arranged by size:
4090 1306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3601 1353
Size: 2248 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2313 4258 4632 4778 387
Size: 1064 138 724 1945 374 146 1280
Fragments arranged by size:
1945 1280 1064 724 374 146 138
EcoICRI GAG'CTC
Cuts at: 2042 2042
Size: 5671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2471 2628 3734 4331 5118 5386 1239
Size: 393 839 157 1106 597 787 268 1524
Fragments arranged by size:
1524 1106 839 787 597 393 268 157
EcoNI CCTnn'n_nnAGG
Cuts at: 2450 2450
Size: 5671
EcoO109I rG'GnC_Cy
Cuts at: 2084 2158 2084
Size: 74 5597
EcoRI G'AATT_C
Cuts at: 2980 3358 2980
Size: 378 5293
EcoRV GAT'ATC
Cuts at: 2389 2389
Size: 5671
FspI TGC'GCA
Cuts at: 4177 4177
Size: 5671
HaeII r_GCGC'y
Cuts at: 1678 2013 4818 1678
Size: 335 2805 2531
Fragments arranged by size:
2805 2531 335
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4312 4344 4386 4418 1373
Size: 32 135 32 2740 32 42 32 2626
Fragments arranged by size:
2740 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2704 3015 3261 669
Size: 988 281 766 311 246 3079
Fragments arranged by size:
3079 988 766 311 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5671
HpaI GTT'AAC
Cuts at: 3261 3261
Size: 5671
KpnI G_GTAC'C
Cuts at: 2996 2996
Size: 5671
MfeI C'AATT_G
Cuts at: 2316 3270 2316
Size: 954 4717
MluI A'CGCG_T
Cuts at: 5547 5547
Size: 5671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4785 4969 1305
Size: 3480 184 2007
Fragments arranged by size:
3480 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2334 10
Size: 54 1425 343 502 3347
Fragments arranged by size:
3347 1425 502 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2369 3629 3988 4147 518
Size: 648 1203 1260 359 159 2042
Fragments arranged by size:
2042 1260 1203 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2242 3087 3764 4912 5157 1606
Size: 636 845 677 1148 245 2120
Fragments arranged by size:
2120 1148 845 677 636 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5119
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5671
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5671
NotI GC'GGCC_GC
Cuts at: 3118 3118
Size: 5671
NspI r_CATG'y
Cuts at: 1132 1229 3029 4574 1132
Size: 97 1800 1545 2229
Fragments arranged by size:
2229 1800 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2555 2848 1348
Size: 1207 293 4171
Fragments arranged by size:
4171 1207 293
PciI A'CATG_T
Cuts at: 4570 4570
Size: 5671
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2311 2735 1108
Size: 1203 424 4044
Fragments arranged by size:
4044 1203 424
PmeI GTTT'AAAC
Cuts at: 2975 2975
Size: 5671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 5253 5285 3740
Size: 32 1481 32 4126
Fragments arranged by size:
4126 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2158 2158
Size: 5671
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5671
PsiI TTA'TAA
Cuts at: 3241 3241
Size: 5671
PstI C_TGCA'G
Cuts at: 830 3023 830
Size: 2193 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5671
PvuII CAG'CTG
Cuts at: 2242 2242
Size: 5671
SacI G_AGCT'C
Cuts at: 2044 2044
Size: 5671
SalI G'TCGA_C
Cuts at: 1936 3013 1936
Size: 1077 4594
SbfI CC_TGCA'GG
Cuts at: 3023 3023
Size: 5671
ScaI AGT'ACT
Cuts at: 3919 5631 3919
Size: 1712 3959
SfcI C'TryA_G
Cuts at: 826 1045 2088 3019 4154 4835 5026 826
Size: 219 1043 931 1135 681 191 1471
Fragments arranged by size:
1471 1135 1043 931 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5671
SmaI CCC'GGG
Cuts at: 1872 2998 1872
Size: 1126 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2114 2539 3775 4676 4938 820
Size: 197 926 171 425 1236 901 262 1553
Fragments arranged by size:
1553 1236 926 901 425 262 197 171
SnaBI TAC'GTA
Cuts at: 493 3462 493
Size: 2969 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5671
SphI G_CATG'C
Cuts at: 1132 3029 1132
Size: 1897 3774
SspI AAT'ATT
Cuts at: 5 52 3595 5
Size: 47 3543 2081
Fragments arranged by size:
3543 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5671
StyI C'CwwG_G
Cuts at: 513 1065 2213 2517 2904 3110 513
Size: 552 1148 304 387 206 3074
Fragments arranged by size:
3074 1148 552 387 304 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2359 2485 3698 3715 3868 4053 4498
Size: 1255 779 126 1213 17 153 185 445
Cuts at: 4498 325
Size: 1498
Fragments arranged by size:
1498 1255 1213 779 445 185 153 126
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3917 5629 96
Size: 274 80 33 51 1443 1940 1712 138
Fragments arranged by size:
1940 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5631 5663 5631
Size: 32 5639
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5671
XbaI T'CTAG_A
Cuts at: 3007 3007
Size: 5671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2354 2490 2354
Size: 136 5535
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5671
XmaI C'CCGG_G
Cuts at: 1870 2996 1870
Size: 1126 4545
XmnI GAAnn'nnTTC
Cuts at: 3800 3800
Size: 5671
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3479 276
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I DraIII FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI