Restriction map for
pFN21AE1508
(Circular) (Six-base) MAPSORT of: pFN21AE1508.seq Check: 2768 from: 1 to: 5131
pFN21AE1508 5131 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2941 278
Size: 53 83 186 936 1405 2468
Fragments arranged by size:
2468 1405 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2474 1937
Size: 537 4594
Acc65I G'GTAC_C
Cuts at: 2452 2452
Size: 5131
AclI AA'CG_TT
Cuts at: 1563 3258 3631 1563
Size: 1695 373 3063
Fragments arranged by size:
3063 1695 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3194 4578 4846 1632
Size: 1562 1384 268 1917
Fragments arranged by size:
1917 1562 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4934
AflIII A'CryG_T
Cuts at: 1203 4030 5007 1203
Size: 2827 977 1327
Fragments arranged by size:
2827 1327 977
AgeI A'CCGG_T
Cuts at: 3962 3962
Size: 5131
AhdI GACnn_n'nnGTC
Cuts at: 3860 3860
Size: 5131
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5131
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5131
AlwNI CAG_nnn'CTG
Cuts at: 2874 4446 2874
Size: 1572 3559
ApaLI G'TGCA_C
Cuts at: 3188 4344 3188
Size: 1156 3975
ApoI r'AATT_y
Cuts at: 1493 2440 2666 2818 4927 1493
Size: 947 226 152 2109 1697
Fragments arranged by size:
2109 1697 947 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3685 160
Size: 872 2653 1606
Fragments arranged by size:
2653 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5131
AvaI C'yCGr_G
Cuts at: 1870 1943 2155 2456 1870
Size: 73 212 301 4545
Fragments arranged by size:
4545 301 212 73
BamHI G'GATC_C
Cuts at: 2461 2828 2461
Size: 367 4764
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2081 2240 2452 3907 618
Size: 325 217 627 294 159 212 1455 1842
Fragments arranged by size:
1842 1455 627 325 294 217 212 159
BanII G_rGCy'C
Cuts at: 1391 2450 1391
Size: 1059 4072
BbeI G_GCGC'C
Cuts at: 2244 2244
Size: 5131
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4160
BbvCI CC'TCA_GC
Cuts at: 2108 2108
Size: 5131
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3322 3356 1903
Size: 34 1385 34 3678
Fragments arranged by size:
3678 1385 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2413 3023 4233 2413
Size: 610 1210 3311
Fragments arranged by size:
3311 1210 610
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5131
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3742 136
Size: 107 122 71 631 605 2070 1525
Fragments arranged by size:
2070 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2055 5126 2055
Size: 3071 2060
BlpI GC'TnA_GC
Cuts at: 2255 2548 2255
Size: 293 4838
Bme1580I G_kGCm'C
Cuts at: 1790 3192 4348 1790
Size: 1402 1156 2573
Fragments arranged by size:
2573 1402 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5131
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2862 3820 442
Size: 416 53 83 399 24 1445 958 1753
Fragments arranged by size:
1753 1445 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5131
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 3791 1239
Size: 875 1677 2579
Fragments arranged by size:
2579 1677 875
Bpu10I CC'TnA_GC
Cuts at: 2108 2108
Size: 5131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3256 4121 4419 3256
Size: 865 298 3968
Fragments arranged by size:
3968 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3794 882
Size: 617 2295 2219
Fragments arranged by size:
2295 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2922 493
Size: 711 1718 2702
Fragments arranged by size:
2702 1718 711
BsaBI GATnn'nnATC
Cuts at: 5125 5125
Size: 5131
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2241 2938 3320 3955
Size: 53 83 186 936 708 697 382 635
Cuts at: 3955 275
Size: 1451
Fragments arranged by size:
1451 936 708 697 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3564 3962 4236 4383 4794 3564
Size: 398 274 147 411 3901
Fragments arranged by size:
3901 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4906
Fragments arranged by size:
4906 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5131
BseYI C'CCAG_C
Cuts at: 4334 4830 4334
Size: 496 4635
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2135 2249 1163
Size: 573 399 114 4045
Fragments arranged by size:
4045 573 399 114
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2364 2400 2581 3342 4370 1540
Size: 275 186 363 36 181 761 1028 2301
Fragments arranged by size:
2301 1028 761 363 275 186 181 36
BsiHKAI G_wGCw'C
Cuts at: 3192 3277 4348 3192
Size: 85 1071 3975
Fragments arranged by size:
3975 1071 85
BsmI GAATG_Cn'
Cuts at: 2642 2735 2642
Size: 93 5038
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2450 3192 3277 4348 1391
Size: 399 660 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 660 399 85
BspEI T'CCGG_A
Cuts at: 4794 4794
Size: 5131
BspHI T'CATG_A
Cuts at: 3020 4750 3020
Size: 1730 3401
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2157 2486 844
Size: 1313 329 3489
Fragments arranged by size:
3489 1313 329
BsrBI CCG'CTC
Cuts at: 1394 1823 3018 1394
Size: 429 1195 3507
Fragments arranged by size:
3507 1195 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3626 3800 65
Size: 1183 2378 174 1396
Fragments arranged by size:
2378 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3775 3962 1700
Size: 110 1965 187 2869
Fragments arranged by size:
2869 1965 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5131
BssHII G'CGCG_C
Cuts at: 1925 2173 2175 2339 1925
Size: 248 2 164 4717
Fragments arranged by size:
4717 248 164 2
BssSI C'ACGA_G
Cuts at: 3191 4203 3191
Size: 1012 4119
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5131
BstBI TT'CG_AA
Cuts at: 3935 3935
Size: 5131
BstEII G'GTnAC_C
Cuts at: 1184 2326 1184
Size: 1142 3989
BstXI CCAn_nnnn'nTGG
Cuts at: 2194 2194
Size: 5131
BstYI r'GATC_y
Cuts at: 1972 2055 2461 2828 3213 3230 4682 4768 4780
Size: 83 406 367 385 17 1452 86 12
Cuts at: 4780 5126 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 406 385 367 346 86 83
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2202 513
Size: 552 1137 3442
Fragments arranged by size:
3442 1137 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4392
Fragments arranged by size:
4392 683 56
BtsI GCAGTG_nn'
Cuts at: 2314 2647 3441 3461 2314
Size: 333 794 20 3984
Fragments arranged by size:
3984 794 333 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5131
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5096
DraI TTT'AAA
Cuts at: 2435 2435
Size: 5131
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4138 809
Size: 507 2822 1802
Fragments arranged by size:
2822 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2196 2334 2361
Size: 54 1425 50 275 18 366 138 27
Cuts at: 2361 2578 3467 5096 8
Size: 217 889 1629 43
Fragments arranged by size:
1629 1425 889 366 275 217 138 54
50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2361 2578 1537
Size: 275 549 217 4090
Fragments arranged by size:
4090 549 275 217
EarI CTCTTCn'nnn_
Cuts at: 1353 2108 2213 3061 1353
Size: 755 105 848 3423
Fragments arranged by size:
3423 848 755 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3718 4092 4238 387
Size: 1064 138 2129 374 146 1280
Fragments arranged by size:
2129 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 3194 3791 4578 4846 1239
Size: 393 482 1080 597 787 268 1524
Fragments arranged by size:
1524 1080 787 597 482 393 268
EcoRI G'AATT_C
Cuts at: 2440 2818 2440
Size: 378 4753
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2149 2149
Size: 5131
FspI TGC'GCA
Cuts at: 3637 3637
Size: 5131
HaeII r_GCGC'y
Cuts at: 1678 2244 4278 1678
Size: 566 2034 2531
Fragments arranged by size:
2531 2034 566
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3772 3804 3846 3878 1373
Size: 32 135 32 2200 32 42 32 2626
Fragments arranged by size:
2626 2200 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2296 2475 2721 669
Size: 988 281 358 179 246 3079
Fragments arranged by size:
3079 988 358 281 246 179
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5131
HpaI GTT'AAC
Cuts at: 2721 2721
Size: 5131
KasI G'GCGC_C
Cuts at: 2240 2240
Size: 5131
KpnI G_GTAC'C
Cuts at: 2456 2456
Size: 5131
MfeI C'AATT_G
Cuts at: 2730 2730
Size: 5131
MluI A'CGCG_T
Cuts at: 5007 5007
Size: 5131
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4245 4429 1305
Size: 2940 184 2007
Fragments arranged by size:
2940 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3309
Fragments arranged by size:
3309 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2036 3089 3448 3607 518
Size: 648 870 1053 359 159 2042
Fragments arranged by size:
2042 1053 870 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2547 3224 4372 4617 1606
Size: 941 677 1148 245 2120
Fragments arranged by size:
2120 1148 941 677 245
NarI GG'CG_CC
Cuts at: 2241 2241
Size: 5131
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4579
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5131
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5131
NotI GC'GGCC_GC
Cuts at: 2578 2578
Size: 5131
NspI r_CATG'y
Cuts at: 1132 1229 2489 4034 1132
Size: 97 1260 1545 2229
Fragments arranged by size:
2229 1545 1260 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5131
PciI A'CATG_T
Cuts at: 4030 4030
Size: 5131
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5131
PmeI GTTT'AAAC
Cuts at: 2435 2435
Size: 5131
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3200 3232 4713 4745 3200
Size: 32 1481 32 3586
Fragments arranged by size:
3586 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5131
PsiI TTA'TAA
Cuts at: 2701 2701
Size: 5131
PstI C_TGCA'G
Cuts at: 830 2483 830
Size: 1653 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5131
SalI G'TCGA_C
Cuts at: 1936 2473 1936
Size: 537 4594
SapI GCTCTTCn'nnn_
Cuts at: 2108 2213 2108
Size: 105 5026
SbfI CC_TGCA'GG
Cuts at: 2483 2483
Size: 5131
ScaI AGT'ACT
Cuts at: 3379 5091 3379
Size: 1712 3419
SfcI C'TryA_G
Cuts at: 826 1045 2479 3614 4295 4486 826
Size: 219 1434 1135 681 191 1471
Fragments arranged by size:
1471 1434 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2242 2242
Size: 5131
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5131
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5131
SmaI CCC'GGG
Cuts at: 1872 2157 2458 1872
Size: 285 301 4545
Fragments arranged by size:
4545 301 285
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3235 4136 4398 820
Size: 197 926 1292 901 262 1553
Fragments arranged by size:
1553 1292 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2922 493
Size: 2429 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5131
SphI G_CATG'C
Cuts at: 1132 2489 1132
Size: 1357 3774
SspI AAT'ATT
Cuts at: 5 52 3055 5
Size: 47 3003 2081
Fragments arranged by size:
3003 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5131
StyI C'CwwG_G
Cuts at: 513 1065 2187 2570 513
Size: 552 1122 383 3074
Fragments arranged by size:
3074 1122 552 383
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2386 2432 3158 3175 3328 3513 3958
Size: 1255 806 46 726 17 153 185 445
Cuts at: 3958 325
Size: 1498
Fragments arranged by size:
1498 1255 806 726 445 185 153 46
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3377 5089 96
Size: 274 80 33 51 1443 1400 1712 138
Fragments arranged by size:
1712 1443 1400 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2144 2176 5091 5123 2144
Size: 32 2915 32 2152
Fragments arranged by size:
2915 2152 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2291 1316
Size: 975 4156
XbaI T'CTAG_A
Cuts at: 2467 2467
Size: 5131
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5131
XmaI C'CCGG_G
Cuts at: 1870 2155 2456 1870
Size: 285 301 4545
Fragments arranged by size:
4545 301 285
XmnI GAAnn'nnTTC
Cuts at: 3260 3260
Size: 5131
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2939 276
Size: 53 83 186 936 1405 2468
Fragments arranged by size:
2468 1405 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI
XcmI