Restriction map for
pFN21AE1505
(Circular) (Six-base) MAPSORT of: pFN21AE1505.seq Check: 4739 from: 1 to: 6193
pFN21AE1505 6193 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2602 2737 2602
Size: 135 6058
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4003 278
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3536 1937
Size: 1599 4594
Acc65I G'GTAC_C
Cuts at: 3514 3514
Size: 6193
AclI AA'CG_TT
Cuts at: 1563 4320 4693 1563
Size: 2757 373 3063
Fragments arranged by size:
3063 2757 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2528 3047 4256 5640 5908 1632
Size: 896 519 1209 1384 268 1917
Fragments arranged by size:
1917 1384 1209 896 519 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5996
AflIII A'CryG_T
Cuts at: 1203 2838 5092 6069 1203
Size: 1635 2254 977 1327
Fragments arranged by size:
2254 1635 1327 977
AgeI A'CCGG_T
Cuts at: 5024 5024
Size: 6193
AhdI GACnn_n'nnGTC
Cuts at: 4922 4922
Size: 6193
AleI CACnn'nnGTG
Cuts at: 1166 2546 1166
Size: 1380 4813
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2564 2937 3242 1184
Size: 1380 373 305 4135
Fragments arranged by size:
4135 1380 373 305
AlwNI CAG_nnn'CTG
Cuts at: 2158 3025 3073 3295 3936 5508 2158
Size: 867 48 222 641 1572 2843
Fragments arranged by size:
2843 1572 867 641 222 48
ApaLI G'TGCA_C
Cuts at: 2673 4250 5406 2673
Size: 1577 1156 3460
Fragments arranged by size:
3460 1577 1156
ApoI r'AATT_y
Cuts at: 1493 3502 3728 3880 5989 1493
Size: 2009 226 152 2109 1697
Fragments arranged by size:
2109 2009 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4747 160
Size: 872 3715 1606
Fragments arranged by size:
3715 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
AvaI C'yCGr_G
Cuts at: 1870 1943 2011 3482 3518 1870
Size: 73 68 1471 36 4545
Fragments arranged by size:
4545 1471 73 68 36
BamHI G'GATC_C
Cuts at: 3523 3890 3523
Size: 367 5826
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3058 3233 3358 3389 3514
Size: 325 217 627 1271 175 125 31 125
Cuts at: 3514 4969 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1271 627 325 217 175 125
125 31
BanII G_rGCy'C
Cuts at: 1391 3512 1391
Size: 2121 4072
BbeI G_GCGC'C
Cuts at: 3237 3237
Size: 6193
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2458 3184 928
Size: 971 559 726 3937
Fragments arranged by size:
3937 971 726 559
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2621 2655 4384 4418 1903
Size: 34 684 34 1729 34 3678
Fragments arranged by size:
3678 1729 684 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4085 5295 4085
Size: 1210 4983
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6193
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2591 4804 136
Size: 107 122 71 631 605 919 2213 1525
Fragments arranged by size:
2213 1525 919 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6188 6188
Size: 6193
BlpI GC'TnA_GC
Cuts at: 3455 3610 3455
Size: 155 6038
Bme1580I G_kGCm'C
Cuts at: 1790 2677 3053 4254 5410 1790
Size: 887 376 1201 1156 2573
Fragments arranged by size:
2573 1201 1156 887 376
BmgBI CAC'GTC
Cuts at: 1318 2410 2656 1318
Size: 1092 246 4855
Fragments arranged by size:
4855 1092 246
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2560 2687 3218
Size: 416 53 83 399 24 1143 127 531
Cuts at: 3218 3924 4882 442
Size: 706 958 1753
Fragments arranged by size:
1753 1143 958 706 531 416 399 127
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6193
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2941 2941
Size: 6193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2168 3356 3467 4853 1239
Size: 929 1188 111 1386 2579
Fragments arranged by size:
2579 1386 1188 929 111
Bpu10I CC'TnA_GC
Cuts at: 3288 3288
Size: 6193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4318 5183 5481 4318
Size: 865 298 5030
Fragments arranged by size:
5030 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3335 4856 882
Size: 617 1836 1521 2219
Fragments arranged by size:
2219 1836 1521 617
BsaAI yAC'GTr
Cuts at: 493 1204 3984 493
Size: 711 2780 2702
Fragments arranged by size:
2780 2702 711
BsaBI GATnn'nnATC
Cuts at: 6187 6187
Size: 6193
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3234 4000 4382 5017
Size: 53 83 186 936 1701 766 382 635
Cuts at: 5017 275
Size: 1451
Fragments arranged by size:
1701 1451 936 766 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2025 3402 4626 5024 5298 5445 5856 2025
Size: 1377 1224 398 274 147 411 2362
Fragments arranged by size:
2362 1377 1224 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2924 2954 1178
Size: 30 165 30 1521 30 4417
Fragments arranged by size:
4417 1521 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2648 3353 1186
Size: 1462 705 4026
Fragments arranged by size:
4026 1462 705
BseYI C'CCAG_C
Cuts at: 2599 3295 5396 5892 2599
Size: 696 2101 496 2900
Fragments arranged by size:
2900 2101 696 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2150 2630 2771 3032 3248 3278 1163
Size: 573 414 480 141 261 216 30 4078
Fragments arranged by size:
4078 573 480 414 261 216 141 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3643 4404 5432 1540
Size: 275 186 1642 761 1028 2301
Fragments arranged by size:
2301 1642 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2018 2553 2677 2883 4254 4339 5410 2018
Size: 535 124 206 1371 85 1071 2801
Fragments arranged by size:
2801 1371 1071 535 206 124 85
BsmI GAATG_Cn'
Cuts at: 3704 3797 3704
Size: 93 6100
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2553 2677 2883 3053 3512 4254
Size: 399 228 535 124 206 170 459 742
Cuts at: 4254 4339 5410 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 535 459 399 228 206
170 124 85
BspEI T'CCGG_A
Cuts at: 3402 5856 3402
Size: 2454 3739
BspHI T'CATG_A
Cuts at: 4082 5812 4082
Size: 1730 4463
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2602 2737 3178 3450 3548 844
Size: 1758 135 441 272 98 3489
Fragments arranged by size:
3489 1758 441 272 135 98
BsrBI CCG'CTC
Cuts at: 1394 1823 2800 3369 4080 1394
Size: 429 977 569 711 3507
Fragments arranged by size:
3507 977 711 569 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4688 4862 65
Size: 1183 3440 174 1396
Fragments arranged by size:
3440 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2522 3055 3344 4837 5024 1700
Size: 110 712 533 289 1493 187 2869
Fragments arranged by size:
2869 1493 712 533 289 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6193
BssHII G'CGCG_C
Cuts at: 1925 3283 1925
Size: 1358 4835
BssSI C'ACGA_G
Cuts at: 2706 4253 5265 2706
Size: 1547 1012 3634
Fragments arranged by size:
3634 1547 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2158 3022 3148 3216 794
Size: 1364 864 126 68 3771
Fragments arranged by size:
3771 1364 864 126 68
BstBI TT'CG_AA
Cuts at: 2793 4997 2793
Size: 2204 3989
BstEII G'GTnAC_C
Cuts at: 1184 2021 1184
Size: 837 5356
BstYI r'GATC_y
Cuts at: 1972 3523 3890 4275 4292 5744 5830 5842 6188
Size: 1551 367 385 17 1452 86 12 346
Cuts at: 6188 1972
Size: 1977
Fragments arranged by size:
1977 1551 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2847 2971 3269 513
Size: 552 939 843 124 298 3437
Fragments arranged by size:
3437 939 843 552 298 124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2502 487
Size: 683 56 1276 4178
Fragments arranged by size:
4178 1276 683 56
BtsI GCAGTG_nn'
Cuts at: 2118 2613 2695 3015 3153 3709 4503 4523 2118
Size: 495 82 320 138 556 794 20 3788
Fragments arranged by size:
3788 794 556 495 320 138 82 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6193
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6158
DraI TTT'AAA
Cuts at: 3497 3497
Size: 6193
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3086 5200 809
Size: 507 1770 2114 1802
Fragments arranged by size:
2114 1802 1770 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2520 2583 2658
Size: 54 1425 50 275 18 690 63 75
Cuts at: 2658 3165 3640 4529 6158 8
Size: 507 475 889 1629 43
Fragments arranged by size:
1629 1425 889 690 507 475 275 75
63 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3640 1537
Size: 275 1828 4090
Fragments arranged by size:
4090 1828 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2790 4123 1353
Size: 1437 1333 3423
Fragments arranged by size:
3423 1437 1333
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2342 2831 4780 5154 5300 387
Size: 1064 138 753 489 1949 374 146 1280
Fragments arranged by size:
1949 1280 1064 753 489 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2168 2528 3047 3356 3467 4256 4853
Size: 393 536 360 519 309 111 789 597
Cuts at: 4853 5640 5908 1239
Size: 787 268 1524
Fragments arranged by size:
1524 789 787 597 536 519 393 360
309 268 111
EcoNI CCTnn'n_nnAGG
Cuts at: 2252 2630 3335 2252
Size: 378 705 5110
Fragments arranged by size:
5110 705 378
EcoO109I rG'GnC_Cy
Cuts at: 2367 3070 2367
Size: 703 5490
EcoRI G'AATT_C
Cuts at: 3502 3880 3502
Size: 378 5815
FspI TGC'GCA
Cuts at: 3123 4699 3123
Size: 1576 4617
HaeII r_GCGC'y
Cuts at: 1678 2651 3237 5340 1678
Size: 973 586 2103 2531
Fragments arranged by size:
2531 2103 973 586
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4834 4866 4908 4940 1373
Size: 32 135 32 3262 32 42 32 2626
Fragments arranged by size:
3262 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2275 3537 3783 669
Size: 988 281 337 1262 246 3079
Fragments arranged by size:
3079 1262 988 337 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6193
HpaI GTT'AAC
Cuts at: 2275 3783 2275
Size: 1508 4685
KasI G'GCGC_C
Cuts at: 3233 3233
Size: 6193
KpnI G_GTAC'C
Cuts at: 3518 3518
Size: 6193
MfeI C'AATT_G
Cuts at: 3792 3792
Size: 6193
MluI A'CGCG_T
Cuts at: 6069 6069
Size: 6193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5307 5491 1305
Size: 4002 184 2007
Fragments arranged by size:
4002 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4371
Fragments arranged by size:
4371 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2546 2888 3353 4151 4510 4669 518
Size: 648 1380 342 465 798 359 159 2042
Fragments arranged by size:
2042 1380 798 648 465 359 342 159
MspA1I CmG'CkG
Cuts at: 1606 2395 2849 2973 3216 3262 3271 3382 3609
Size: 789 454 124 243 46 9 111 227
Cuts at: 3609 4286 5434 5679 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 789 677 454 245 243 227
124 111 46 9
NaeI GCC'GGC
Cuts at: 2524 2524
Size: 6193
NarI GG'CG_CC
Cuts at: 3234 3234
Size: 6193
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4702
Fragments arranged by size:
4702 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6193
NgoMIV G'CCGG_C
Cuts at: 2522 2522
Size: 6193
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6193
NotI GC'GGCC_GC
Cuts at: 3640 3640
Size: 6193
NspI r_CATG'y
Cuts at: 1132 1229 3551 5096 1132
Size: 97 2322 1545 2229
Fragments arranged by size:
2322 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6193
PciI A'CATG_T
Cuts at: 5092 5092
Size: 6193
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2260 2362 1108
Size: 1152 102 4939
Fragments arranged by size:
4939 1152 102
PfoI T'CCnGG_A
Cuts at: 2400 3333 2400
Size: 933 5260
PmeI GTTT'AAAC
Cuts at: 3497 3497
Size: 6193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2951 2983 4262 4294 5775 5807 2951
Size: 32 1279 32 1481 32 3337
Fragments arranged by size:
3337 1481 1279 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6193
PsiI TTA'TAA
Cuts at: 3763 3763
Size: 6193
PspXI vC'TCGA_Gb
Cuts at: 2011 3482 2011
Size: 1471 4722
PstI C_TGCA'G
Cuts at: 830 3010 3545 830
Size: 2180 535 3478
Fragments arranged by size:
3478 2180 535
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6193
PvuII CAG'CTG
Cuts at: 2395 3216 3262 2395
Size: 821 46 5326
Fragments arranged by size:
5326 821 46
SacII CC_GC'GG
Cuts at: 2850 2974 3272 2850
Size: 124 298 5771
Fragments arranged by size:
5771 298 124
SalI G'TCGA_C
Cuts at: 1936 3535 1936
Size: 1599 4594
SapI GCTCTTCn'nnn_
Cuts at: 2790 2790
Size: 6193
SbfI CC_TGCA'GG
Cuts at: 3545 3545
Size: 6193
ScaI AGT'ACT
Cuts at: 3488 4441 6153 3488
Size: 953 1712 3528
Fragments arranged by size:
3528 1712 953
SexAI A'CCwGG_T
Cuts at: 3081 3081
Size: 6193
SfcI C'TryA_G
Cuts at: 826 1045 2371 3006 3541 4676 5357 5548 826
Size: 219 1326 635 535 1135 681 191 1471
Fragments arranged by size:
1471 1326 1135 681 635 535 219 191
SfoI GGC'GCC
Cuts at: 3235 3235
Size: 6193
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
SgrAI Cr'CCGG_yG
Cuts at: 1700 3055 1700
Size: 1355 4838
SmaI CCC'GGG
Cuts at: 1872 3520 1872
Size: 1648 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2011 3482 4297 5198 5460 820
Size: 197 926 68 1471 815 901 262 1553
Fragments arranged by size:
1553 1471 926 901 815 262 197 68
SnaBI TAC'GTA
Cuts at: 493 3984 493
Size: 3491 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6193
SphI G_CATG'C
Cuts at: 1132 3551 1132
Size: 2419 3774
SspI AAT'ATT
Cuts at: 5 52 4117 5
Size: 47 4065 2081
Fragments arranged by size:
4065 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6193
StyI C'CwwG_G
Cuts at: 513 1065 2004 2139 2943 3168 3423 3632 513
Size: 552 939 135 804 225 255 209 3074
Fragments arranged by size:
3074 939 804 552 255 225 209 135
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4220 4237 4390 4575 5020 325
Size: 1255 2640 17 153 185 445 1498
Fragments arranged by size:
2640 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3240 3486 4439
Size: 274 80 33 51 1443 1263 246 953
Cuts at: 4439 6151 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1263 953 274 246 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2924 2956 6153 6185 2924
Size: 32 3197 32 2932
Fragments arranged by size:
3197 2932 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6193
XbaI T'CTAG_A
Cuts at: 3529 3529
Size: 6193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2950 2950
Size: 6193
XhoI C'TCGA_G
Cuts at: 1943 2011 3482 1943
Size: 68 1471 4654
Fragments arranged by size:
4654 1471 68
XmaI C'CCGG_G
Cuts at: 1870 3518 1870
Size: 1648 4545
XmnI GAAnn'nnTTC
Cuts at: 2180 4322 2180
Size: 2142 4051
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4001 276
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PpuMI
PspOMI PsrI RsrII SacI SanDI SfiI SrfI SwaI