Restriction map for
pFN21AE1504
(Circular) (Six-base) MAPSORT of: pFN21AE1504.seq Check: 3553 from: 1 to: 8212
pFN21AE1504 8212 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2239 3919 2239
Size: 1680 6532
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4656 5106 6022 278
Size: 53 83 186 936 3120 450 916 2468
Fragments arranged by size:
3120 2468 936 916 450 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2297 3405 3663 4776 5555 1937
Size: 360 1108 258 1113 779 4594
Fragments arranged by size:
4594 1113 1108 779 360 258
Acc65I G'GTAC_C
Cuts at: 5533 5533
Size: 8212
AclI AA'CG_TT
Cuts at: 1563 3985 6339 6712 1563
Size: 2422 2354 373 3063
Fragments arranged by size:
3063 2422 2354 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3011 3055 4148 4225 4342 5098 5165 6275
Size: 1379 44 1093 77 117 756 67 1110
Cuts at: 6275 7659 7927 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1379 1110 1093 756 268 117
77 67 44
AfeI AGC'GCT
Cuts at: 2107 3076 2107
Size: 969 7243
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8015
AflIII A'CryG_T
Cuts at: 1203 2271 2361 3180 4544 7111 8088 1203
Size: 1068 90 819 1364 2567 977 1327
Fragments arranged by size:
2567 1364 1327 1068 977 819 90
AgeI A'CCGG_T
Cuts at: 7043 7043
Size: 8212
AhdI GACnn_n'nnGTC
Cuts at: 6941 6941
Size: 8212
AleI CACnn'nnGTG
Cuts at: 1166 3317 4217 5234 1166
Size: 2151 900 1017 4144
Fragments arranged by size:
4144 2151 1017 900
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8212
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2447 2479 2447
Size: 32 8180
AlwNI CAG_nnn'CTG
Cuts at: 2320 3262 3766 3949 4832 5027 5955 7527 2320
Size: 942 504 183 883 195 928 1572 3005
Fragments arranged by size:
3005 1572 942 928 883 504 195 183
ApaI G_GGCC'C
Cuts at: 2396 2743 3277 3445 3634 3823 2396
Size: 347 534 168 189 189 6785
Fragments arranged by size:
6785 534 347 189 189 168
ApaLI G'TGCA_C
Cuts at: 2339 2711 6269 7425 2339
Size: 372 3558 1156 3126
Fragments arranged by size:
3558 3126 1156 372
ApoI r'AATT_y
Cuts at: 1493 2591 2618 2760 2890 5521 5747 5899 8008
Size: 1098 27 142 130 2631 226 152 2109
Cuts at: 8008 1493
Size: 1697
Fragments arranged by size:
2631 2109 1697 1098 226 152 142 130
27
AseI AT'TA_AT
Cuts at: 160 1032 6766 160
Size: 872 5734 1606
Fragments arranged by size:
5734 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8212
AvaI C'yCGr_G
Cuts at: 1870 1943 3465 3822 4121 4162 5537 1870
Size: 73 1522 357 299 41 1375 4545
Fragments arranged by size:
4545 1522 1375 357 299 73 41
BamHI G'GATC_C
Cuts at: 5542 5909 5542
Size: 367 7845
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2225 2251 3137 4574 4622
Size: 325 217 627 438 26 886 1437 48
Cuts at: 4622 4921 5533 6988 618
Size: 299 612 1455 1842
Fragments arranged by size:
1842 1455 1437 886 627 612 438 325
299 217 48 26
BanII G_rGCy'C
Cuts at: 1391 2164 2238 2396 2743 3167 3277 3445 3634
Size: 773 74 158 347 424 110 168 189
Cuts at: 3634 3728 3823 4758 5278 5531 1391
Size: 94 95 935 520 253 4072
Fragments arranged by size:
4072 935 773 520 424 347 253 189
168 158 110 95 94 74
BbeI G_GCGC'C
Cuts at: 4626 4626
Size: 8212
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4446 928
Size: 971 2547 4694
Fragments arranged by size:
4694 2547 971
BbvCI CC'TCA_GC
Cuts at: 2642 5156 2642
Size: 2514 5698
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5272 5306 6403 6437 1903
Size: 34 3335 34 1097 34 3678
Fragments arranged by size:
3678 3335 1097 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6104 7314 6104
Size: 1210 7002
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8212
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6823 136
Size: 107 122 71 631 605 5151 1525
Fragments arranged by size:
5151 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3000 8207 3000
Size: 5207 3005
BlpI GC'TnA_GC
Cuts at: 5629 5629
Size: 8212
Bme1580I G_kGCm'C
Cuts at: 1790 2256 2343 2396 2441 2715 2743 3277 3445
Size: 466 87 53 45 274 28 534 168
Cuts at: 3445 3634 3823 4577 6273 7429 1790
Size: 189 189 754 1696 1156 2573
Fragments arranged by size:
2573 1696 1156 754 534 466 274 189
189 168 87 53 45 28
BmgBI CAC'GTC
Cuts at: 1318 3619 1318
Size: 2301 5911
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2557 4553 5943
Size: 416 53 83 399 24 1140 1996 1390
Cuts at: 5943 6901 442
Size: 958 1753
Fragments arranged by size:
1996 1753 1390 1140 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8212
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3153 3724 4421 4600 4772 5315 6872 1239
Size: 1914 571 697 179 172 543 1557 2579
Fragments arranged by size:
2579 1914 1557 697 571 543 179 172
Bpu10I CC'TnA_GC
Cuts at: 2044 2479 2642 3842 5156 2044
Size: 435 163 1200 1314 5100
Fragments arranged by size:
5100 1314 1200 435 163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6337 7202 7500 6337
Size: 865 298 7049
Fragments arranged by size:
7049 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2545 3017 3940 4671 4972 6875 882
Size: 617 1046 472 923 731 301 1903 2219
Fragments arranged by size:
2219 1903 1046 923 731 617 472 301
BsaAI yAC'GTr
Cuts at: 493 1204 4498 6003 493
Size: 711 3294 1505 2702
Fragments arranged by size:
3294 2702 1505 711
BsaBI GATnn'nnATC
Cuts at: 8206 8206
Size: 8212
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2294 4623 4653 5103
Size: 53 83 186 936 761 2329 30 450
Cuts at: 5103 6019 6401 7036 275
Size: 916 382 635 1451
Fragments arranged by size:
2329 1451 936 916 761 635 450 382
186 83 53 30
BsaWI w'CCGG_w
Cuts at: 2372 4095 6645 7043 7317 7464 7875 2372
Size: 1723 2550 398 274 147 411 2709
Fragments arranged by size:
2709 2550 1723 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2109 2139 4934 4964 5093
Size: 30 165 30 706 30 2795 30 129
Cuts at: 5093 5123 1178
Size: 30 4267
Fragments arranged by size:
4267 2795 706 165 129 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2018 2093 2482 3727 4850 4990 5141 5444
Size: 832 75 389 1245 1123 140 151 303
Cuts at: 5444 1186
Size: 3954
Fragments arranged by size:
3954 1245 1123 832 389 303 151 140
75
BseYI C'CCAG_C
Cuts at: 2108 2313 2400 2555 3586 3689 3729 5020 5166
Size: 205 87 155 1031 103 40 1291 146
Cuts at: 5166 5244 5474 7415 7911 2108
Size: 78 230 1941 496 2409
Fragments arranged by size:
2409 1941 1291 1031 496 230 205 155
146 103 87 78 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3275 4520 1163
Size: 573 1539 1245 4855
Fragments arranged by size:
4855 1539 1245 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5202 5662 6423 7451 1540
Size: 275 186 3201 460 761 1028 2301
Fragments arranged by size:
3201 2301 1028 761 460 275 186
BsiHKAI G_wGCw'C
Cuts at: 2343 2715 3837 3861 4586 4758 5000 5278 6273
Size: 372 1122 24 725 172 242 278 995
Cuts at: 6273 6358 7429 2343
Size: 85 1071 3126
Fragments arranged by size:
3126 1122 1071 995 725 372 278 242
172 85 24
BsmI GAATG_Cn'
Cuts at: 5723 5816 5723
Size: 93 8119
BsmBI CGTCTCn'nnnn_
Cuts at: 4660 4660
Size: 8212
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2164 2238 2256 2343 2396 2441 2715
Size: 399 374 74 18 87 53 45 274
Cuts at: 2715 2743 3167 3277 3445 3634 3728 3823 3837
Size: 28 424 110 168 189 94 95 14
Cuts at: 3837 3861 4577 4586 4758 5000 5278 5531 6273
Size: 24 716 9 172 242 278 253 742
Cuts at: 6273 6358 7429 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 716 424 399 374 278
274 253 242 189 172 168 110 95
94 87 85 74 53 45 28 24
18 14 9
BspEI T'CCGG_A
Cuts at: 7875 7875
Size: 8212
BspHI T'CATG_A
Cuts at: 6101 7831 6101
Size: 1730 6482
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2239 3898 3919 4005 4161 4257 4905 5567
Size: 1395 1659 21 86 156 96 648 662
Cuts at: 5567 844
Size: 3489
Fragments arranged by size:
3489 1659 1395 662 648 156 96 86
21
BsrBI CCG'CTC
Cuts at: 1394 1823 3771 6099 1394
Size: 429 1948 2328 3507
Fragments arranged by size:
3507 2328 1948 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2913 3335 5073 6707 6881 65
Size: 1183 1665 422 1738 1634 174 1396
Fragments arranged by size:
1738 1665 1634 1396 1183 422 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4680 6856 7043 1700
Size: 110 2870 2176 187 2869
Fragments arranged by size:
2870 2869 2176 187 110
BsrGI T'GTAC_A
Cuts at: 96 5385 96
Size: 5289 2923
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8212
BssSI C'ACGA_G
Cuts at: 2345 6272 7284 2345
Size: 3927 1012 3273
Fragments arranged by size:
3927 3273 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4590 794
Size: 3796 4416
BstBI TT'CG_AA
Cuts at: 3045 7016 3045
Size: 3971 4241
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8212
BstXI CCAn_nnnn'nTGG
Cuts at: 2195 3235 2195
Size: 1040 7172
BstYI r'GATC_y
Cuts at: 1972 3000 3897 5542 5909 6294 6311 7763 7849
Size: 1028 897 1645 367 385 17 1452 86
Cuts at: 7849 7861 8207 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1645 1452 1028 897 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5499 5499
Size: 8212
BtgI C'CryG_G
Cuts at: 513 1065 2826 3396 4615 513
Size: 552 1761 570 1219 4110
Fragments arranged by size:
4110 1761 1219 570 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7473
Fragments arranged by size:
7473 683 56
BtsI GCAGTG_nn'
Cuts at: 4714 5728 6522 6542 4714
Size: 1014 794 20 6384
Fragments arranged by size:
6384 1014 794 20
ClaI AT'CG_AT
Cuts at: 1554 2472 1554
Size: 918 7294
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8177
DraI TTT'AAA
Cuts at: 2947 5516 2947
Size: 2569 5643
DraIII CAC_nnn'GTG
Cuts at: 4139 4139
Size: 8212
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2126 4901 7219 809
Size: 507 810 2775 2318 1802
Fragments arranged by size:
2775 2318 1802 810 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2688 4054 4263
Size: 54 1425 50 275 18 858 1366 209
Cuts at: 4263 4854 5659 6548 8177 8
Size: 591 805 889 1629 43
Fragments arranged by size:
1629 1425 1366 889 858 805 591 275
209 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5659 1537
Size: 275 3847 4090
Fragments arranged by size:
4090 3847 275
EarI CTCTTCn'nnn_
Cuts at: 1353 6142 1353
Size: 4789 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5162 6799 7173 7319 387
Size: 1064 138 3573 1637 374 146 1280
Fragments arranged by size:
3573 1637 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4756 5276 4756
Size: 520 7692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3011 3055 3153 3724 4148 4225 4342
Size: 393 1379 44 98 571 424 77 117
Cuts at: 4342 4421 4600 4772 5098 5165 5315 6275 6872
Size: 79 179 172 326 67 150 960 597
Cuts at: 6872 7659 7927 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1379 960 787 597 571 424 393
326 268 179 172 150 117 98 79
77 67 44
EcoNI CCTnn'n_nnAGG
Cuts at: 3496 3788 3496
Size: 292 7920
EcoO109I rG'GnC_Cy
Cuts at: 2393 2739 3273 3274 3381 3441 3442 3630 3631
Size: 346 534 1 107 60 1 188 1
Cuts at: 3631 3686 3819 4894 2393
Size: 55 133 1075 5711
Fragments arranged by size:
5711 1075 534 346 188 133 107 60
55 1 1 1
EcoRI G'AATT_C
Cuts at: 2618 5521 5899 2618
Size: 2903 378 4931
Fragments arranged by size:
4931 2903 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4759 4759
Size: 8212
FspI TGC'GCA
Cuts at: 6718 6718
Size: 8212
HaeII r_GCGC'y
Cuts at: 1678 2109 3078 4557 4626 7359 1678
Size: 431 969 1479 69 2733 2531
Fragments arranged by size:
2733 2531 1479 969 431 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2903 2935 3678 3710 3760
Size: 32 135 32 1331 32 743 32 50
Cuts at: 3760 3792 3843 3875 4101 4133 4857 4889 5093
Size: 32 51 32 226 32 724 32 204
Cuts at: 5093 5125 6853 6885 6927 6959 1373
Size: 32 1728 32 42 32 2626
Fragments arranged by size:
2626 1728 1331 743 724 226 204 135
51 50 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5556 5802 669
Size: 988 281 3618 246 3079
Fragments arranged by size:
3618 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8212
HpaI GTT'AAC
Cuts at: 5802 5802
Size: 8212
KasI G'GCGC_C
Cuts at: 4622 4622
Size: 8212
KpnI G_GTAC'C
Cuts at: 5537 5537
Size: 8212
MfeI C'AATT_G
Cuts at: 2859 5811 2859
Size: 2952 5260
MluI A'CGCG_T
Cuts at: 8088 8088
Size: 8212
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2137 2610 2848 3984 7326 7510 1305
Size: 832 473 238 1136 3342 184 2007
Fragments arranged by size:
3342 2007 1136 832 473 238 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4856 10
Size: 54 1425 343 3024 3366
Fragments arranged by size:
3366 3024 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2513 2825 3179 3317 4217 5234 6170
Size: 648 1347 312 354 138 900 1017 936
Cuts at: 6170 6529 6688 518
Size: 359 159 2042
Fragments arranged by size:
2042 1347 1017 936 900 648 359 354
312 159 138
MspA1I CmG'CkG
Cuts at: 1606 2180 2317 2668 3715 4525 4590 4750 5097
Size: 574 137 351 1047 810 65 160 347
Cuts at: 5097 5378 5628 6305 7453 7698 1606
Size: 281 250 677 1148 245 2120
Fragments arranged by size:
2120 1148 1047 810 677 574 351 347
281 250 245 160 137 65
NarI GG'CG_CC
Cuts at: 4623 4623
Size: 8212
NcoI C'CATG_G
Cuts at: 513 1065 2826 513
Size: 552 1761 5899
Fragments arranged by size:
5899 1761 552
NdeI CA'TA_TG
Cuts at: 387 2734 387
Size: 2347 5865
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8212
NotI GC'GGCC_GC
Cuts at: 5659 5659
Size: 8212
NspI r_CATG'y
Cuts at: 1132 1229 2275 2365 3184 3880 4548 5570 7115
Size: 97 1046 90 819 696 668 1022 1545
Cuts at: 7115 1132
Size: 2229
Fragments arranged by size:
2229 1545 1046 1022 819 696 668 97
90
PasI CC'CwG_GG
Cuts at: 1348 2278 3498 3555 1348
Size: 930 1220 57 6005
Fragments arranged by size:
6005 1220 930 57
PciI A'CATG_T
Cuts at: 2271 2361 3180 4544 7111 2271
Size: 90 819 1364 2567 3372
Fragments arranged by size:
3372 2567 1364 819 90
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2145 2320 3330 3504 1108
Size: 1037 175 1010 174 5816
Fragments arranged by size:
5816 1037 1010 175 174
PfoI T'CCnGG_A
Cuts at: 4080 5121 5251 4080
Size: 1041 130 7041
Fragments arranged by size:
7041 1041 130
PmeI GTTT'AAAC
Cuts at: 5516 5516
Size: 8212
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6281 6313 7794 7826 6281
Size: 32 1481 32 6667
Fragments arranged by size:
6667 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3686 4894 3686
Size: 1208 7004
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8212
PsiI TTA'TAA
Cuts at: 2811 5782 2811
Size: 2971 5241
PspOMI G'GGCC_C
Cuts at: 2392 2739 3273 3441 3630 3819 2392
Size: 347 534 168 189 189 6785
Fragments arranged by size:
6785 534 347 189 189 168
PstI C_TGCA'G
Cuts at: 830 2250 3750 4595 5564 830
Size: 1420 1500 845 969 3478
Fragments arranged by size:
3478 1500 1420 969 845
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8212
PvuII CAG'CTG
Cuts at: 2180 2317 2668 4525 4590 4750 2180
Size: 137 351 1857 65 160 5642
Fragments arranged by size:
5642 1857 351 160 137 65
SacI G_AGCT'C
Cuts at: 4758 5278 4758
Size: 520 7692
SalI G'TCGA_C
Cuts at: 1936 5554 1936
Size: 3618 4594
SanDI GG'GwC_CC
Cuts at: 3686 3686
Size: 8212
SbfI CC_TGCA'GG
Cuts at: 2250 5564 2250
Size: 3314 4898
ScaI AGT'ACT
Cuts at: 6460 8172 6460
Size: 1712 6500
SexAI A'CCwGG_T
Cuts at: 3125 3426 4869 3125
Size: 301 1443 6468
Fragments arranged by size:
6468 1443 301
SfcI C'TryA_G
Cuts at: 826 1045 2215 2246 3746 4591 5560 6695 7376
Size: 219 1170 31 1500 845 969 1135 681
Cuts at: 7376 7567 826
Size: 191 1471
Fragments arranged by size:
1500 1471 1170 1135 969 845 681 219
191 31
SfoI GGC'GCC
Cuts at: 4624 4624
Size: 8212
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8212
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8212
SmaI CCC'GGG
Cuts at: 1872 3824 5539 1872
Size: 1952 1715 4545
Fragments arranged by size:
4545 1952 1715
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6316 7217 7479 820
Size: 197 926 4373 901 262 1553
Fragments arranged by size:
4373 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6003 493
Size: 5510 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8212
SphI G_CATG'C
Cuts at: 1132 3880 5570 1132
Size: 2748 1690 3774
Fragments arranged by size:
3774 2748 1690
SspI AAT'ATT
Cuts at: 5 52 6136 5
Size: 47 6084 2081
Fragments arranged by size:
6084 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8212
StyI C'CwwG_G
Cuts at: 513 1065 2826 4032 4662 5651 513
Size: 552 1761 1206 630 989 3074
Fragments arranged by size:
3074 1761 1206 989 630 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2137 2414 2423 2576 3329 3803 3853
Size: 1255 557 277 9 153 753 474 50
Cuts at: 3853 4909 5188 6239 6256 6409 6594 7039 325
Size: 1056 279 1051 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1056 1051 753 557 474 445
279 277 185 153 153 50 17 9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5238 5301 5385
Size: 274 80 33 51 1443 3261 63 84
Cuts at: 5385 5412 6458 8170 96
Size: 27 1046 1712 138
Fragments arranged by size:
3261 1712 1443 1046 274 138 84 80
63 51 33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2109 2141 4934 4966 8172 8204 2109
Size: 32 2793 32 3206 32 2117
Fragments arranged by size:
3206 2793 2117 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3428 3620 5507 1316
Size: 2112 192 1887 4021
Fragments arranged by size:
4021 2112 1887 192
XbaI T'CTAG_A
Cuts at: 5548 5548
Size: 8212
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2146 2732 4669 4747 2146
Size: 586 1937 78 5611
Fragments arranged by size:
5611 1937 586 78
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8212
XmaI C'CCGG_G
Cuts at: 1870 3822 5537 1870
Size: 1952 1715 4545
Fragments arranged by size:
4545 1952 1715
XmnI GAAnn'nnTTC
Cuts at: 6341 6341
Size: 8212
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4654 5104 6020 276
Size: 53 83 186 936 3120 450 916 2468
Fragments arranged by size:
3120 2468 936 916 450 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SapI SfiI SrfI SwaI