Restriction map for
pFN21AE1502
(Circular) (Six-base) MAPSORT of: pFN21AE1502.seq Check: 2529 from: 1 to: 5800
pFN21AE1502 5800 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2717 2717
Size: 5800
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3610 278
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3143 1937
Size: 1206 4594
Acc65I G'GTAC_C
Cuts at: 3121 3121
Size: 5800
AclI AA'CG_TT
Cuts at: 1563 3927 4300 1563
Size: 2364 373 3063
Fragments arranged by size:
3063 2364 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3863 5247 5515 1632
Size: 2231 1384 268 1917
Fragments arranged by size:
2231 1917 1384 268
AfeI AGC'GCT
Cuts at: 2762 2762
Size: 5800
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5603
AflIII A'CryG_T
Cuts at: 1203 2779 4699 5676 1203
Size: 1576 1920 977 1327
Fragments arranged by size:
1920 1576 1327 977
AgeI A'CCGG_T
Cuts at: 4631 4631
Size: 5800
AhdI GACnn_n'nnGTC
Cuts at: 2548 4529 2548
Size: 1981 3819
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5800
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2825 1184
Size: 1641 4159
AlwNI CAG_nnn'CTG
Cuts at: 2124 2722 2961 3093 3543 5115 2124
Size: 598 239 132 450 1572 2809
Fragments arranged by size:
2809 1572 598 450 239 132
ApaI G_GGCC'C
Cuts at: 2428 2428
Size: 5800
ApaLI G'TGCA_C
Cuts at: 3857 5013 3857
Size: 1156 4644
ApoI r'AATT_y
Cuts at: 1493 3109 3335 3487 5596 1493
Size: 1616 226 152 2109 1697
Fragments arranged by size:
2109 1697 1616 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4354 160
Size: 872 3322 1606
Fragments arranged by size:
3322 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 2202 2216 3125 1870
Size: 73 87 172 14 909 4545
Fragments arranged by size:
4545 909 172 87 73 14
BamHI G'GATC_C
Cuts at: 3130 3497 3130
Size: 367 5433
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2207 2348 2410 2453 2771
Size: 325 217 627 420 141 62 43 318
Cuts at: 2771 2789 2988 3121 4576 618
Size: 18 199 133 1455 1842
Fragments arranged by size:
1842 1455 627 420 325 318 217 199
141 133 62 43 18
BanII G_rGCy'C
Cuts at: 1391 2428 2483 3087 3119 1391
Size: 1037 55 604 32 4072
Fragments arranged by size:
4072 1037 604 55 32
BbeI G_GCGC'C
Cuts at: 2211 2352 2414 2775 2211
Size: 141 62 361 5236
Fragments arranged by size:
5236 361 141 62
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4829
BbvCI CC'TCA_GC
Cuts at: 2954 2954
Size: 5800
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3991 4025 1903
Size: 34 50 34 1970 34 3678
Fragments arranged by size:
3678 1970 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3692 4902 3692
Size: 1210 4590
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5800
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2419 2451 2478
Size: 107 122 71 631 605 747 32 27
Cuts at: 2478 4411 136
Size: 1933 1525
Fragments arranged by size:
1933 1525 747 631 605 122 107 71
32 27
BglII A'GATC_T
Cuts at: 5795 5795
Size: 5800
BlpI GC'TnA_GC
Cuts at: 2175 3086 3217 2175
Size: 911 131 4758
Fragments arranged by size:
4758 911 131
Bme1580I G_kGCm'C
Cuts at: 1790 2060 2428 2792 2991 3861 5017 1790
Size: 270 368 364 199 870 1156 2573
Fragments arranged by size:
2573 1156 870 368 364 270 199
BmgBI CAC'GTC
Cuts at: 1318 2068 2530 1318
Size: 750 462 4588
Fragments arranged by size:
4588 750 462
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3531 4489 442
Size: 416 53 83 399 24 2114 958 1753
Fragments arranged by size:
2114 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2201 1056
Size: 1145 4655
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2870 2870
Size: 5800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2251 2777 4460 1239
Size: 1012 526 1683 2579
Fragments arranged by size:
2579 1683 1012 526
Bpu10I CC'TnA_GC
Cuts at: 2954 2954
Size: 5800
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2816 3925 4790 5088 2816
Size: 1109 865 298 3528
Fragments arranged by size:
3528 1109 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4463 882
Size: 617 2964 2219
Fragments arranged by size:
2964 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3591 493
Size: 711 2387 2702
Fragments arranged by size:
2702 2387 711
BsaBI GATnn'nnATC
Cuts at: 5794 5794
Size: 5800
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2208 2349 2411 2772
Size: 53 83 186 936 675 141 62 361
Cuts at: 2772 3607 3989 4624 275
Size: 835 382 635 1451
Fragments arranged by size:
1451 936 835 675 635 382 361 186
141 83 62 53
BsaWI w'CCGG_w
Cuts at: 4233 4631 4905 5052 5463 4233
Size: 398 274 147 411 4570
Fragments arranged by size:
4570 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5575
Fragments arranged by size:
5575 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2846 1186
Size: 1660 4140
BseYI C'CCAG_C
Cuts at: 5003 5499 5003
Size: 496 5304
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2600 3026 1163
Size: 573 864 426 3937
Fragments arranged by size:
3937 864 573 426
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2346 2926 2983 3250 4011 5039
Size: 275 186 345 580 57 267 761 1028
Cuts at: 5039 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 580 345 275 267 186
57
BsiHKAI G_wGCw'C
Cuts at: 3087 3861 3946 5017 3087
Size: 774 85 1071 3870
Fragments arranged by size:
3870 1071 774 85
BsmI GAATG_Cn'
Cuts at: 3311 3404 3311
Size: 93 5707
BsmBI CGTCTCn'nnnn_
Cuts at: 2309 2309
Size: 5800
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2428 2483 2792 2991 3087 3119
Size: 399 270 368 55 309 199 96 32
Cuts at: 3119 3861 3946 5017 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 368 309 270 199
96 85 55 32
BspEI T'CCGG_A
Cuts at: 5463 5463
Size: 5800
BspHI T'CATG_A
Cuts at: 3689 5419 3689
Size: 1730 4070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2717 2976 3155 844
Size: 1873 259 179 3489
Fragments arranged by size:
3489 1873 259 179
BsrBI CCG'CTC
Cuts at: 1394 1823 2849 3687 1394
Size: 429 1026 838 3507
Fragments arranged by size:
3507 1026 838 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4295 4469 65
Size: 1183 3047 174 1396
Fragments arranged by size:
3047 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2345 2903 4444 4631 1700
Size: 110 535 558 1541 187 2869
Fragments arranged by size:
2869 1541 558 535 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5800
BssHII G'CGCG_C
Cuts at: 1925 2697 2745 1925
Size: 772 48 4980
Fragments arranged by size:
4980 772 48
BssSI C'ACGA_G
Cuts at: 3860 4872 3860
Size: 1012 4788
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2667 2722 794
Size: 1873 55 3872
Fragments arranged by size:
3872 1873 55
BstBI TT'CG_AA
Cuts at: 4604 4604
Size: 5800
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5800
BstYI r'GATC_y
Cuts at: 1972 3130 3497 3882 3899 5351 5437 5449 5795
Size: 1158 367 385 17 1452 86 12 346
Cuts at: 5795 1972
Size: 1977
Fragments arranged by size:
1977 1452 1158 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2416 2475 2677 2767 2983 513
Size: 552 1351 59 202 90 216 3330
Fragments arranged by size:
3330 1351 552 216 202 90 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5061
Fragments arranged by size:
5061 683 56
BtsI GCAGTG_nn'
Cuts at: 2337 2402 3316 4110 4130 2337
Size: 65 914 794 20 4007
Fragments arranged by size:
4007 914 794 65 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5800
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5765
DraI TTT'AAA
Cuts at: 3104 3104
Size: 5800
DraIII CAC_nnn'GTG
Cuts at: 2667 2667
Size: 5800
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4807 809
Size: 507 3491 1802
Fragments arranged by size:
3491 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2343 2640
Size: 54 1425 50 275 18 315 198 297
Cuts at: 2640 2980 3247 4136 5765 8
Size: 340 267 889 1629 43
Fragments arranged by size:
1629 1425 889 340 315 297 275 267
198 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2343 2980 3247 1537
Size: 275 531 637 267 4090
Fragments arranged by size:
4090 637 531 275 267
EarI CTCTTCn'nnn_
Cuts at: 1353 3009 3730 1353
Size: 1656 721 3423
Fragments arranged by size:
3423 1656 721
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2088 2240 4387 4761 4907 387
Size: 1064 138 499 152 2147 374 146 1280
Fragments arranged by size:
2147 1280 1064 499 374 152 146 138
EcoICRI GAG'CTC
Cuts at: 3085 3085
Size: 5800
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2251 2777 3863 4460 5247 5515 1239
Size: 393 619 526 1086 597 787 268 1524
Fragments arranged by size:
1524 1086 787 619 597 526 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2300 2300
Size: 5800
EcoO109I rG'GnC_Cy
Cuts at: 2425 2806 2425
Size: 381 5419
EcoRI G'AATT_C
Cuts at: 3109 3487 3109
Size: 378 5422
FspI TGC'GCA
Cuts at: 2705 3077 4306 2705
Size: 372 1229 4199
Fragments arranged by size:
4199 1229 372
HaeII r_GCGC'y
Cuts at: 1678 2211 2352 2414 2499 2764 2775 2946 4947
Size: 533 141 62 85 265 11 171 2001
Cuts at: 4947 1678
Size: 2531
Fragments arranged by size:
2531 2001 533 265 171 141 85 62
11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2534 2566 4441 4473 4515
Size: 32 135 32 962 32 1875 32 42
Cuts at: 4515 4547 1373
Size: 32 2626
Fragments arranged by size:
2626 1875 962 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3144 3390 669
Size: 988 281 1206 246 3079
Fragments arranged by size:
3079 1206 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5800
HpaI GTT'AAC
Cuts at: 3390 3390
Size: 5800
KasI G'GCGC_C
Cuts at: 2207 2348 2410 2771 2207
Size: 141 62 361 5236
Fragments arranged by size:
5236 361 141 62
KpnI G_GTAC'C
Cuts at: 3125 3125
Size: 5800
MfeI C'AATT_G
Cuts at: 3399 3399
Size: 5800
MluI A'CGCG_T
Cuts at: 2779 5676 2779
Size: 2897 2903
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4914 5098 1305
Size: 3609 184 2007
Fragments arranged by size:
3609 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3978
Fragments arranged by size:
3978 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3758 4117 4276 518
Size: 648 2592 359 159 2042
Fragments arranged by size:
2592 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2256 2418 2477 2958 2985 3066 3216 3893
Size: 650 162 59 481 27 81 150 677
Cuts at: 3893 5041 5286 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 650 481 245 162 150
81 59 27
NaeI GCC'GGC
Cuts at: 2347 2347
Size: 5800
NarI GG'CG_CC
Cuts at: 2208 2349 2411 2772 2208
Size: 141 62 361 5236
Fragments arranged by size:
5236 361 141 62
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5248
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5800
NgoMIV G'CCGG_C
Cuts at: 2345 2345
Size: 5800
NheI G'CTAG_C
Cuts at: 1052 2197 1052
Size: 1145 4655
NotI GC'GGCC_GC
Cuts at: 2980 3247 2980
Size: 267 5533
NruI TCG'CGA
Cuts at: 2736 2736
Size: 5800
NspI r_CATG'y
Cuts at: 1132 1229 3158 4703 1132
Size: 97 1929 1545 2229
Fragments arranged by size:
2229 1929 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5800
PciI A'CATG_T
Cuts at: 4699 4699
Size: 5800
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5800
PmeI GTTT'AAAC
Cuts at: 3104 3104
Size: 5800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2219 2251 3869 3901 5382 5414 2219
Size: 32 1618 32 1481 32 2605
Fragments arranged by size:
2605 1618 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5800
PsiI TTA'TAA
Cuts at: 3370 3370
Size: 5800
PspOMI G'GGCC_C
Cuts at: 2424 2424
Size: 5800
PstI C_TGCA'G
Cuts at: 830 2129 2397 3152 830
Size: 1299 268 755 3478
Fragments arranged by size:
3478 1299 755 268
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5800
SacI G_AGCT'C
Cuts at: 3087 3087
Size: 5800
SacII CC_GC'GG
Cuts at: 2419 2478 2986 2419
Size: 59 508 5233
Fragments arranged by size:
5233 508 59
SalI G'TCGA_C
Cuts at: 1936 3142 1936
Size: 1206 4594
SbfI CC_TGCA'GG
Cuts at: 3152 3152
Size: 5800
ScaI AGT'ACT
Cuts at: 4048 5760 4048
Size: 1712 4088
SfcI C'TryA_G
Cuts at: 826 1045 2125 2393 3148 4283 4964 5155 826
Size: 219 1080 268 755 1135 681 191 1471
Fragments arranged by size:
1471 1135 1080 755 681 268 219 191
SfoI GGC'GCC
Cuts at: 2209 2350 2412 2773 2209
Size: 141 62 361 5236
Fragments arranged by size:
5236 361 141 62
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5800
SmaI CCC'GGG
Cuts at: 1872 3127 1872
Size: 1255 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2831 3904 4805 5067 820
Size: 197 926 888 1073 901 262 1553
Fragments arranged by size:
1553 1073 926 901 888 262 197
SnaBI TAC'GTA
Cuts at: 493 3591 493
Size: 3098 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5800
SphI G_CATG'C
Cuts at: 1132 3158 1132
Size: 2026 3774
SspI AAT'ATT
Cuts at: 5 52 3724 5
Size: 47 3672 2081
Fragments arranged by size:
3672 2081 47
StuI AGG'CCT
Cuts at: 1517 2282 2372 1517
Size: 765 90 4945
Fragments arranged by size:
4945 765 90
StyI C'CwwG_G
Cuts at: 513 1065 2100 3239 513
Size: 552 1035 1139 3074
Fragments arranged by size:
3074 1139 1035 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3827 3844 3997 4182 4627 325
Size: 1255 2247 17 153 185 445 1498
Fragments arranged by size:
2247 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3052 4046 5758
Size: 274 80 33 51 1443 1075 994 1712
Cuts at: 5758 96
Size: 138
Fragments arranged by size:
1712 1443 1075 994 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5760 5792 5760
Size: 32 5768
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5800
XbaI T'CTAG_A
Cuts at: 3136 3136
Size: 5800
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5800
XmaI C'CCGG_G
Cuts at: 1870 3125 1870
Size: 1255 4545
XmnI GAAnn'nnTTC
Cuts at: 3929 3929
Size: 5800
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3608 276
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI SacI SacII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I
Bsu36I EcoRV FalI FseI FspAI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI PvuII RsrII SanDI SapI
SexAI SfiI SrfI SwaI XcmI