Restriction map for
pFN21AE1501
(Circular) (Six-base) MAPSORT of: pFN21AE1501.seq Check: 60 from: 1 to: 5554
pFN21AE1501 5554 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2064 2796 3364 278
Size: 53 83 186 936 528 732 568 2468
Fragments arranged by size:
2468 936 732 568 528 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2897 1937
Size: 960 4594
Acc65I G'GTAC_C
Cuts at: 2875 2875
Size: 5554
AclI AA'CG_TT
Cuts at: 1563 3681 4054 1563
Size: 2118 373 3063
Fragments arranged by size:
3063 2118 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3617 5001 5269 1632
Size: 1985 1384 268 1917
Fragments arranged by size:
1985 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5357
AflIII A'CryG_T
Cuts at: 1203 4453 5430 1203
Size: 3250 977 1327
Fragments arranged by size:
3250 1327 977
AgeI A'CCGG_T
Cuts at: 4385 4385
Size: 5554
AhdI GACnn_n'nnGTC
Cuts at: 4283 4283
Size: 5554
AleI CACnn'nnGTG
Cuts at: 1166 2801 1166
Size: 1635 3919
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2300 1184
Size: 1116 4438
AlwNI CAG_nnn'CTG
Cuts at: 3297 4869 3297
Size: 1572 3982
ApaLI G'TGCA_C
Cuts at: 3611 4767 3611
Size: 1156 4398
ApoI r'AATT_y
Cuts at: 1493 2863 3089 3241 5350 1493
Size: 1370 226 152 2109 1697
Fragments arranged by size:
2109 1697 1370 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4108 160
Size: 872 3076 1606
Fragments arranged by size:
3076 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5554
AvaI C'yCGr_G
Cuts at: 1870 1943 2440 2503 2879 1870
Size: 73 497 63 376 4545
Fragments arranged by size:
4545 497 376 73 63
BamHI G'GATC_C
Cuts at: 2884 3251 2884
Size: 367 5187
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2246 2465 2875 4330 618
Size: 325 217 627 459 219 410 1455 1842
Fragments arranged by size:
1842 1455 627 459 410 325 219 217
BanII G_rGCy'C
Cuts at: 1391 2101 2156 2846 2873 1391
Size: 710 55 690 27 4072
Fragments arranged by size:
4072 710 690 55 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4583
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3745 3779 1903
Size: 34 1808 34 3678
Fragments arranged by size:
3678 1808 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3446 4656 3446
Size: 1210 4344
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5554
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2244 2780 4165
Size: 107 122 71 631 605 572 536 1385
Cuts at: 4165 136
Size: 1525
Fragments arranged by size:
1525 1385 631 605 572 536 122 107
71
BglII A'GATC_T
Cuts at: 5549 5549
Size: 5554
BlpI GC'TnA_GC
Cuts at: 2971 2971
Size: 5554
Bme1580I G_kGCm'C
Cuts at: 1790 2222 3615 4771 1790
Size: 432 1393 1156 2573
Fragments arranged by size:
2573 1393 1156 432
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5554
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2032 2141 2614
Size: 416 53 83 399 24 615 109 473
Cuts at: 2614 3285 4243 442
Size: 671 958 1753
Fragments arranged by size:
1753 958 671 615 473 416 399 109
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2037 2059 2226 2323 2419 2552 4214 1239
Size: 798 22 167 97 96 133 1662 2579
Fragments arranged by size:
2579 1662 798 167 133 97 96 22
Bpu10I CC'TnA_GC
Cuts at: 2273 2273
Size: 5554
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2086 3679 4544 4842 2086
Size: 1593 865 298 2798
Fragments arranged by size:
2798 1593 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4217 882
Size: 617 2718 2219
Fragments arranged by size:
2718 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3345 493
Size: 711 2141 2702
Fragments arranged by size:
2702 2141 711
BsaBI GATnn'nnATC
Cuts at: 5548 5548
Size: 5554
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2061 2793 3361 3743
Size: 53 83 186 936 528 732 568 382
Cuts at: 3743 4378 275
Size: 635 1451
Fragments arranged by size:
1451 936 732 635 568 528 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2788 3987 4385 4659 4806 5217 2788
Size: 1199 398 274 147 411 3125
Fragments arranged by size:
3125 1199 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2126 2156 2417 2447 2500
Size: 30 165 30 723 30 261 30 53
Cuts at: 2500 2530 1178
Size: 30 4202
Fragments arranged by size:
4202 723 261 165 53 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2022 2828 2854 1186
Size: 836 806 26 3886
Fragments arranged by size:
3886 836 806 26
BseYI C'CCAG_C
Cuts at: 2113 2305 2585 4757 5253 2113
Size: 192 280 2172 496 2414
Fragments arranged by size:
2414 2172 496 280 192
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2194 2474 2578 1163
Size: 573 458 280 104 4139
Fragments arranged by size:
4139 573 458 280 104
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3004 3765 4793 1540
Size: 275 186 1003 761 1028 2301
Fragments arranged by size:
2301 1028 1003 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3615 3700 4771 3615
Size: 85 1071 4398
Fragments arranged by size:
4398 1071 85
BsmI GAATG_Cn'
Cuts at: 3065 3158 3065
Size: 93 5461
BsmBI CGTCTCn'nnnn_
Cuts at: 2725 2725
Size: 5554
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2101 2156 2222 2846 2873 3615 3700
Size: 399 311 55 66 624 27 742 85
Cuts at: 3700 4771 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 624 399 311 85 66
55 27
BspEI T'CCGG_A
Cuts at: 2788 5217 2788
Size: 2429 3125
BspHI T'CATG_A
Cuts at: 3443 5173 3443
Size: 1730 3824
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2909 844
Size: 2065 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3441 1394
Size: 429 1618 3507
Fragments arranged by size:
3507 1618 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2253 4049 4223 65
Size: 1183 1005 1796 174 1396
Fragments arranged by size:
1796 1396 1183 1005 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4198 4385 1700
Size: 110 2388 187 2869
Fragments arranged by size:
2869 2388 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5554
BssHII G'CGCG_C
Cuts at: 1925 2320 1925
Size: 395 5159
BssSI C'ACGA_G
Cuts at: 3614 4626 3614
Size: 1012 4542
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5554
BstBI TT'CG_AA
Cuts at: 4358 4358
Size: 5554
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5554
BstXI CCAn_nnnn'nTGG
Cuts at: 2267 2267
Size: 5554
BstYI r'GATC_y
Cuts at: 1972 2042 2884 3251 3636 3653 5105 5191 5203
Size: 70 842 367 385 17 1452 86 12
Cuts at: 5203 5549 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 842 385 367 346 86 70
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2310 2799 2808 513
Size: 552 939 306 489 9 3259
Fragments arranged by size:
3259 939 552 489 306 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4815
Fragments arranged by size:
4815 683 56
BtsI GCAGTG_nn'
Cuts at: 2294 2404 3070 3864 3884 2294
Size: 110 666 794 20 3964
Fragments arranged by size:
3964 794 666 110 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5554
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2749 2784 529
Size: 35 2185 35 3299
Fragments arranged by size:
3299 2185 35 35
DraI TTT'AAA
Cuts at: 2858 2858
Size: 5554
DraIII CAC_nnn'GTG
Cuts at: 2226 2487 2226
Size: 261 5293
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4561 809
Size: 507 3245 1802
Fragments arranged by size:
3245 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2805 3001 3890
Size: 54 1425 50 275 18 975 196 889
Cuts at: 3890 5519 8
Size: 1629 43
Fragments arranged by size:
1629 1425 975 889 275 196 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3001 1537
Size: 275 1189 4090
Fragments arranged by size:
4090 1189 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3484 1353
Size: 2131 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4141 4515 4661 387
Size: 1064 138 2552 374 146 1280
Fragments arranged by size:
2552 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2037 2059 2226 2323 2419 2552 3617
Size: 393 405 22 167 97 96 133 1065
Cuts at: 3617 4214 5001 5269 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1065 787 597 405 393 268 167
133 97 96 22
EcoO109I rG'GnC_Cy
Cuts at: 2027 2508 2526 2773 2782 2027
Size: 481 18 247 9 4799
Fragments arranged by size:
4799 481 247 18 9
EcoRI G'AATT_C
Cuts at: 2863 3241 2863
Size: 378 5176
FspI TGC'GCA
Cuts at: 4060 4060
Size: 5554
HaeII r_GCGC'y
Cuts at: 1678 4701 1678
Size: 3023 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2500 2532 4195 4227 4269
Size: 32 135 32 928 32 1663 32 42
Cuts at: 4269 4301 1373
Size: 32 2626
Fragments arranged by size:
2626 1663 928 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2898 3144 669
Size: 988 281 960 246 3079
Fragments arranged by size:
3079 988 960 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5554
HpaI GTT'AAC
Cuts at: 3144 3144
Size: 5554
KpnI G_GTAC'C
Cuts at: 2879 2879
Size: 5554
MfeI C'AATT_G
Cuts at: 3153 3153
Size: 5554
MluI A'CGCG_T
Cuts at: 5430 5430
Size: 5554
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4668 4852 1305
Size: 3363 184 2007
Fragments arranged by size:
3363 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3732
Fragments arranged by size:
3732 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2265 2675 2801 3512 3871 4030 518
Size: 648 1099 410 126 711 359 159 2042
Fragments arranged by size:
2042 1099 711 648 410 359 159 126
MspA1I CmG'CkG
Cuts at: 1606 2562 2731 2970 3647 4795 5040 1606
Size: 956 169 239 677 1148 245 2120
Fragments arranged by size:
2120 1148 956 677 245 239 169
NcoI C'CATG_G
Cuts at: 513 1065 2004 2310 513
Size: 552 939 306 3757
Fragments arranged by size:
3757 939 552 306
NdeI CA'TA_TG
Cuts at: 387 2672 387
Size: 2285 3269
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5554
NotI GC'GGCC_GC
Cuts at: 3001 3001
Size: 5554
NspI r_CATG'y
Cuts at: 1132 1229 2912 4457 1132
Size: 97 1683 1545 2229
Fragments arranged by size:
2229 1683 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5554
PciI A'CATG_T
Cuts at: 4453 4453
Size: 5554
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5554
PfoI T'CCnGG_A
Cuts at: 2014 2339 2391 2014
Size: 325 52 5177
Fragments arranged by size:
5177 325 52
PmeI GTTT'AAAC
Cuts at: 2858 2858
Size: 5554
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3623 3655 5136 5168 3623
Size: 32 1481 32 4009
Fragments arranged by size:
4009 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2508 2526 2508
Size: 18 5536
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5554
PsiI TTA'TAA
Cuts at: 3124 3124
Size: 5554
PstI C_TGCA'G
Cuts at: 830 2289 2369 2781 2906 830
Size: 1459 80 412 125 3478
Fragments arranged by size:
3478 1459 412 125 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5554
PvuII CAG'CTG
Cuts at: 2562 2562
Size: 5554
SalI G'TCGA_C
Cuts at: 1936 2896 1936
Size: 960 4594
SanDI GG'GwC_CC
Cuts at: 2526 2526
Size: 5554
SbfI CC_TGCA'GG
Cuts at: 2781 2906 2781
Size: 125 5429
ScaI AGT'ACT
Cuts at: 3802 5514 3802
Size: 1712 3842
SfcI C'TryA_G
Cuts at: 826 1045 2285 2365 2777 2902 4037 4718 4909
Size: 219 1240 80 412 125 1135 681 191
Cuts at: 4909 826
Size: 1471
Fragments arranged by size:
1471 1240 1135 681 412 219 191 125
80
SfiI GGCCn_nnn'nGGCC
Cuts at: 2780 2780
Size: 5554
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5554
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5554
SmaI CCC'GGG
Cuts at: 1872 2442 2505 2881 1872
Size: 570 63 376 4545
Fragments arranged by size:
4545 570 376 63
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2065 3658 4559 4821 820
Size: 197 926 122 1593 901 262 1553
Fragments arranged by size:
1593 1553 926 901 262 197 122
SnaBI TAC'GTA
Cuts at: 493 3345 493
Size: 2852 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5554
SphI G_CATG'C
Cuts at: 1132 2912 1132
Size: 1780 3774
SrfI GCCC'GGGC
Cuts at: 2442 2442
Size: 5554
SspI AAT'ATT
Cuts at: 5 52 3478 5
Size: 47 3426 2081
Fragments arranged by size:
3426 2081 47
StuI AGG'CCT
Cuts at: 1517 2201 1517
Size: 684 4870
StyI C'CwwG_G
Cuts at: 513 1065 2004 2310 2693 2993 513
Size: 552 939 306 383 300 3074
Fragments arranged by size:
3074 939 552 383 306 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3581 3598 3751 3936 4381 325
Size: 1255 2001 17 153 185 445 1498
Fragments arranged by size:
2001 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3800 5512 96
Size: 274 80 33 51 1443 1823 1712 138
Fragments arranged by size:
1823 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2471 2503 5514 5546 2471
Size: 32 3011 32 2479
Fragments arranged by size:
3011 2479 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5554
XbaI T'CTAG_A
Cuts at: 2890 2890
Size: 5554
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2527 2806 2527
Size: 279 5275
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5554
XmaI C'CCGG_G
Cuts at: 1870 2440 2503 2879 1870
Size: 570 63 376 4545
Fragments arranged by size:
4545 570 376 63
XmnI GAAnn'nnTTC
Cuts at: 3683 3683
Size: 5554
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2062 2794 3362 276
Size: 53 83 186 936 528 732 568 2468
Fragments arranged by size:
2468 936 732 568 528 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SapI SexAI SfoI SwaI