Restriction map for
pFN21AE1500
(Circular) (Six-base) MAPSORT of: pFN21AE1500.seq Check: 1809 from: 1 to: 7006
pFN21AE1500 7006 bp
With 182 enzymes: SgfI *
August 26, 2010 19:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4816 278
Size: 53 83 186 936 3280 2468
Fragments arranged by size:
3280 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4349 1937
Size: 2412 4594
Acc65I G'GTAC_C
Cuts at: 4327 4327
Size: 7006
AclI AA'CG_TT
Cuts at: 1563 3370 5133 5506 1563
Size: 1807 1763 373 3063
Fragments arranged by size:
3063 1807 1763 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2039 3428 5069 6453 6721 1632
Size: 407 1389 1641 1384 268 1917
Fragments arranged by size:
1917 1641 1389 1384 407 268
AflII C'TTAA_G
Cuts at: 820 1017 2680 820
Size: 197 1663 5146
Fragments arranged by size:
5146 1663 197
AflIII A'CryG_T
Cuts at: 1203 2950 4102 5905 6882 1203
Size: 1747 1152 1803 977 1327
Fragments arranged by size:
1803 1747 1327 1152 977
AgeI A'CCGG_T
Cuts at: 5837 5837
Size: 7006
AhdI GACnn_n'nnGTC
Cuts at: 5735 5735
Size: 7006
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7006
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3209 3225 3351 1184
Size: 2025 16 126 4839
Fragments arranged by size:
4839 2025 126 16
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2790 2822 3779 3811 2790
Size: 32 957 32 5985
Fragments arranged by size:
5985 957 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2185 3544 4749 6321 2185
Size: 1359 1205 1572 2870
Fragments arranged by size:
2870 1572 1359 1205
ApaI G_GGCC'C
Cuts at: 3728 3728
Size: 7006
ApaLI G'TGCA_C
Cuts at: 5063 6219 5063
Size: 1156 5850
ApoI r'AATT_y
Cuts at: 1493 2318 2992 4115 4315 4541 4693 6802 1493
Size: 825 674 1123 200 226 152 2109 1697
Fragments arranged by size:
2109 1697 1123 825 674 226 200 152
AseI AT'TA_AT
Cuts at: 160 1032 5560 160
Size: 872 4528 1606
Fragments arranged by size:
4528 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7006
AvaI C'yCGr_G
Cuts at: 1870 1943 3066 3772 4331 1870
Size: 73 1123 706 559 4545
Fragments arranged by size:
4545 1123 706 559 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2195 2228 4286 4319 2195
Size: 33 2058 33 4882
Fragments arranged by size:
4882 2058 33 33
BamHI G'GATC_C
Cuts at: 2076 4336 4703 2076
Size: 2260 367 4379
Fragments arranged by size:
4379 2260 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4036 4327 5782 618
Size: 325 217 627 2249 291 1455 1842
Fragments arranged by size:
2249 1842 1455 627 325 291 217
BanII G_rGCy'C
Cuts at: 1391 2808 3165 3728 3945 3993 4325 1391
Size: 1417 357 563 217 48 332 4072
Fragments arranged by size:
4072 1417 563 357 332 217 48
BbeI G_GCGC'C
Cuts at: 4040 4040
Size: 7006
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2880 3956 928
Size: 971 981 1076 3978
Fragments arranged by size:
3978 1076 981 971
BbvCI CC'TCA_GC
Cuts at: 3713 3713
Size: 7006
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3763 3797 5197 5231 1903
Size: 34 1826 34 1400 34 3678
Fragments arranged by size:
3678 1826 1400 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2995 4287 4898 6108 2995
Size: 1292 611 1210 3893
Fragments arranged by size:
3893 1292 1210 611
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7006
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2363 5617 136
Size: 107 122 71 631 605 691 3254 1525
Fragments arranged by size:
3254 1525 691 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2167 7001 2167
Size: 4834 2172
BlpI GC'TnA_GC
Cuts at: 2985 4423 2985
Size: 1438 5568
Bme1580I G_kGCm'C
Cuts at: 1790 3728 5067 6223 1790
Size: 1938 1339 1156 2573
Fragments arranged by size:
2573 1938 1339 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7006
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2452 4737 5695
Size: 416 53 83 399 24 1035 2285 958
Cuts at: 5695 442
Size: 1753
Fragments arranged by size:
2285 1753 1035 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7006
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2143 3080 3101 3236 3290 3344 3482 3554
Size: 904 937 21 135 54 54 138 72
Cuts at: 3554 3575 3617 3671 3917 3959 4238 5666 1239
Size: 21 42 54 246 42 279 1428 2579
Fragments arranged by size:
2579 1428 937 904 279 246 138 135
72 54 54 54 42 42 21 21
Bpu10I CC'TnA_GC
Cuts at: 3042 3713 3042
Size: 671 6335
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2847 3438 3653 4190 5131 5996 6294 2847
Size: 591 215 537 941 865 298 3559
Fragments arranged by size:
3559 941 865 591 537 298 215
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5669 882
Size: 617 4170 2219
Fragments arranged by size:
4170 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2953 3766 4797 493
Size: 711 1749 813 1031 2702
Fragments arranged by size:
2702 1749 1031 813 711
BsaBI GATnn'nnATC
Cuts at: 7000 7000
Size: 7006
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4037 4813 5195 5830
Size: 53 83 186 936 2504 776 382 635
Cuts at: 5830 275
Size: 1451
Fragments arranged by size:
2504 1451 936 776 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3945 5439 5837 6111 6258 6669 3945
Size: 1494 398 274 147 411 4282
Fragments arranged by size:
4282 1494 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2790 2820 3209 3239 1178
Size: 30 165 30 1387 30 389 30 4945
Fragments arranged by size:
4945 1387 389 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2660 3119 3479 3692 4205 4208 1186
Size: 1474 459 360 213 513 3 3984
Fragments arranged by size:
3984 1474 513 459 360 213 3
BseYI C'CCAG_C
Cuts at: 2178 6209 6705 2178
Size: 4031 496 2479
Fragments arranged by size:
4031 2479 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2784 3215 1163
Size: 573 1048 431 4954
Fragments arranged by size:
4954 1048 573 431
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4274 4456 5217 6245 1540
Size: 275 186 2273 182 761 1028 2301
Fragments arranged by size:
2301 2273 1028 761 275 186 182
BsiHKAI G_wGCw'C
Cuts at: 2808 3165 3945 3993 5067 5152 6223 2808
Size: 357 780 48 1074 85 1071 3591
Fragments arranged by size:
3591 1074 1071 780 357 85 48
BsmI GAATG_Cn'
Cuts at: 3282 3356 4517 4610 3282
Size: 74 1161 93 5678
Fragments arranged by size:
5678 1161 93 74
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2808 3165 3728 3945 3993 4325 5067
Size: 399 1018 357 563 217 48 332 742
Cuts at: 5067 5152 6223 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1018 742 563 399 357 332
217 85 48
BspEI T'CCGG_A
Cuts at: 3945 6669 3945
Size: 2724 4282
BspHI T'CATG_A
Cuts at: 4895 6625 4895
Size: 1730 5276
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3717 4361 844
Size: 2873 644 3489
Fragments arranged by size:
3489 2873 644
BsrBI CCG'CTC
Cuts at: 1394 1823 4893 1394
Size: 429 3070 3507
Fragments arranged by size:
3507 3070 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3728 5501 5675 65
Size: 1183 2480 1773 174 1396
Fragments arranged by size:
2480 1773 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5650 5837 1700
Size: 110 3840 187 2869
Fragments arranged by size:
3840 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7006
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7006
BssSI C'ACGA_G
Cuts at: 5066 6078 5066
Size: 1012 5994
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3091 3934 794
Size: 2297 843 3866
Fragments arranged by size:
3866 2297 843
BstBI TT'CG_AA
Cuts at: 3996 5810 3996
Size: 1814 5192
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7006
BstYI r'GATC_y
Cuts at: 1972 2076 2167 3847 4336 4703 5088 5105 6557
Size: 104 91 1680 489 367 385 17 1452
Cuts at: 6557 6643 6655 7001 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1680 1452 489 385 367 346 104
91 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3659 3659
Size: 7006
BtgI C'CryG_G
Cuts at: 513 1065 2081 3447 3843 4298 513
Size: 552 1016 1366 396 455 3221
Fragments arranged by size:
3221 1366 1016 552 455 396
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6267
Fragments arranged by size:
6267 683 56
BtsI GCAGTG_nn'
Cuts at: 2850 3693 4522 5316 5336 2850
Size: 843 829 794 20 4520
Fragments arranged by size:
4520 843 829 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7006
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6971
DraI TTT'AAA
Cuts at: 2571 4113 4310 2571
Size: 1542 197 5267
Fragments arranged by size:
5267 1542 197
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6013 809
Size: 507 4697 1802
Fragments arranged by size:
4697 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3444 4271 4453
Size: 54 1425 50 275 18 1614 827 182
Cuts at: 4453 5342 6971 8
Size: 889 1629 43
Fragments arranged by size:
1629 1614 1425 889 827 275 182 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4271 4453 1537
Size: 275 2459 182 4090
Fragments arranged by size:
4090 2459 275 182
EarI CTCTTCn'nnn_
Cuts at: 1353 2031 2721 2912 3480 3998 4936 1353
Size: 678 690 191 568 518 938 3423
Fragments arranged by size:
3423 938 690 678 568 518 191
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3689 5593 5967 6113 387
Size: 1064 138 2100 1904 374 146 1280
Fragments arranged by size:
2100 1904 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2806 3163 3943 3991 2806
Size: 357 780 48 5821
Fragments arranged by size:
5821 780 357 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2143 3080 3101 3236 3290 3344
Size: 393 407 104 937 21 135 54 54
Cuts at: 3344 3428 3482 3554 3575 3617 3671 3917 3959
Size: 84 54 72 21 42 54 246 42
Cuts at: 3959 4238 5069 5666 6453 6721 1239
Size: 279 831 597 787 268 1524
Fragments arranged by size:
1524 937 831 787 597 407 393 279
268 246 135 104 84 72 54 54
54 54 42 42 21 21
EcoNI CCTnn'n_nnAGG
Cuts at: 3464 3464
Size: 7006
EcoO109I rG'GnC_Cy
Cuts at: 3205 3655 3663 3706 3724 3787 3205
Size: 450 8 43 18 63 6424
Fragments arranged by size:
6424 450 63 43 18 8
EcoRI G'AATT_C
Cuts at: 4315 4693 4315
Size: 378 6628
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2291 2291
Size: 7006
FspI TGC'GCA
Cuts at: 5512 5512
Size: 7006
HaeII r_GCGC'y
Cuts at: 1678 3248 4040 6153 1678
Size: 1570 792 2113 2531
Fragments arranged by size:
2531 2113 1570 792
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2159 2191 2473 2505 3000
Size: 32 135 32 587 32 282 32 495
Cuts at: 3000 3032 5647 5679 5721 5753 1373
Size: 32 2615 32 42 32 2626
Fragments arranged by size:
2626 2615 587 495 282 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4350 4596 669
Size: 988 281 2412 246 3079
Fragments arranged by size:
3079 2412 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2273 748
Size: 1525 5481
HpaI GTT'AAC
Cuts at: 4596 4596
Size: 7006
KasI G'GCGC_C
Cuts at: 4036 4036
Size: 7006
KpnI G_GTAC'C
Cuts at: 4331 4331
Size: 7006
MfeI C'AATT_G
Cuts at: 4605 4605
Size: 7006
MluI A'CGCG_T
Cuts at: 6882 6882
Size: 7006
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3139 3830 6120 6304 1305
Size: 1834 691 2290 184 2007
Fragments arranged by size:
2290 2007 1834 691 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3446 10
Size: 54 1425 343 1614 3570
Fragments arranged by size:
3570 1614 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4964 5323 5482 518
Size: 648 3798 359 159 2042
Fragments arranged by size:
3798 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3287 3595 3637 3931 4422 5099 6247 6492
Size: 1681 308 42 294 491 677 1148 245
Cuts at: 6492 1606
Size: 2120
Fragments arranged by size:
2120 1681 1148 677 491 308 294 245
42
NarI GG'CG_CC
Cuts at: 4037 4037
Size: 7006
NcoI C'CATG_G
Cuts at: 513 1065 3447 3843 4298 513
Size: 552 2382 396 455 3221
Fragments arranged by size:
3221 2382 552 455 396
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7006
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7006
NotI GC'GGCC_GC
Cuts at: 4453 4453
Size: 7006
NruI TCG'CGA
Cuts at: 4131 4131
Size: 7006
NspI r_CATG'y
Cuts at: 1132 1229 2566 4106 4204 4364 5909 1132
Size: 97 1337 1540 98 160 1545 2229
Fragments arranged by size:
2229 1545 1540 1337 160 98 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7006
PciI A'CATG_T
Cuts at: 4102 5905 4102
Size: 1803 5203
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3262 1108
Size: 2154 4852
PfoI T'CCnGG_A
Cuts at: 4002 4002
Size: 7006
PmeI GTTT'AAAC
Cuts at: 4310 4310
Size: 7006
PmlI CAC'GTG
Cuts at: 2953 3766 2953
Size: 813 6193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2790 2822 5075 5107 6588 6620 2790
Size: 32 2253 32 1481 32 3176
Fragments arranged by size:
3176 2253 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3663 3706 3787 3663
Size: 43 81 6882
Fragments arranged by size:
6882 81 43
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7006
PsiI TTA'TAA
Cuts at: 4154 4576 4154
Size: 422 6584
PspOMI G'GGCC_C
Cuts at: 3724 3724
Size: 7006
PspXI vC'TCGA_Gb
Cuts at: 3772 3772
Size: 7006
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3360 3392 3360
Size: 32 6974
PstI C_TGCA'G
Cuts at: 830 3627 3642 3688 3814 3960 4358 830
Size: 2797 15 46 126 146 398 3478
Fragments arranged by size:
3478 2797 398 146 126 46 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7006
PvuII CAG'CTG
Cuts at: 3287 3595 3637 3287
Size: 308 42 6656
Fragments arranged by size:
6656 308 42
SacI G_AGCT'C
Cuts at: 2808 3165 3945 3993 2808
Size: 357 780 48 5821
Fragments arranged by size:
5821 780 357 48
SalI G'TCGA_C
Cuts at: 1936 4348 1936
Size: 2412 4594
SapI GCTCTTCn'nnn_
Cuts at: 2721 3998 2721
Size: 1277 5729
SbfI CC_TGCA'GG
Cuts at: 4358 4358
Size: 7006
ScaI AGT'ACT
Cuts at: 5254 6966 5254
Size: 1712 5294
SfcI C'TryA_G
Cuts at: 826 1045 3623 3638 3684 3810 3860 3956 4354
Size: 219 2578 15 46 126 50 96 398
Cuts at: 4354 5489 6170 6361 826
Size: 1135 681 191 1471
Fragments arranged by size:
2578 1471 1135 681 398 219 191 126
96 50 46 15
SfoI GGC'GCC
Cuts at: 4038 4038
Size: 7006
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7006
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7006
SmaI CCC'GGG
Cuts at: 1872 4333 1872
Size: 2461 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2680 2862 3453 3668 3772 4205
Size: 197 926 737 182 591 215 104 433
Cuts at: 4205 5110 6011 6273 820
Size: 905 901 262 1553
Fragments arranged by size:
1553 926 905 901 737 591 433 262
215 197 182 104
SnaBI TAC'GTA
Cuts at: 493 4797 493
Size: 4304 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7006
SphI G_CATG'C
Cuts at: 1132 4364 1132
Size: 3232 3774
SspI AAT'ATT
Cuts at: 5 52 2446 4930 5
Size: 47 2394 2484 2081
Fragments arranged by size:
2484 2394 2081 47
StuI AGG'CCT
Cuts at: 1517 3098 1517
Size: 1581 5425
StyI C'CwwG_G
Cuts at: 513 1065 2910 3447 3843 4257 4298 4445 513
Size: 552 1845 537 396 414 41 147 3074
Fragments arranged by size:
3074 1845 552 537 414 396 147 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3254 5033 5050 5203 5388 5833 325
Size: 1255 1674 1779 17 153 185 445 1498
Fragments arranged by size:
1779 1674 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5252 6964 96
Size: 274 80 33 51 1443 3275 1712 138
Fragments arranged by size:
3275 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6966 6998 6966
Size: 32 6974
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7006
XbaI T'CTAG_A
Cuts at: 4342 4342
Size: 7006
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3055 3055
Size: 7006
XhoI C'TCGA_G
Cuts at: 1943 3772 1943
Size: 1829 5177
XmaI C'CCGG_G
Cuts at: 1870 4331 1870
Size: 2461 4545
XmnI GAAnn'nnTTC
Cuts at: 2492 3564 5135 2492
Size: 1072 1571 4363
Fragments arranged by size:
4363 1571 1072
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4814 276
Size: 53 83 186 936 3280 2468
Fragments arranged by size:
3280 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NruI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BfrBI BplI BsiWI BsmBI
BstXI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV
NsiI PacI RsrII SacII SanDI SexAI SfiI SrfI
SwaI