Restriction map for
pFN21AE1493
(Circular) (Six-base) MAPSORT of: pFN21AE1493.seq Check: 3057 from: 1 to: 5581
pFN21AE1493 5581 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3391 278
Size: 53 83 186 936 1855 2468
Fragments arranged by size:
2468 1855 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2924 1937
Size: 987 4594
Acc65I G'GTAC_C
Cuts at: 2902 2902
Size: 5581
AclI AA'CG_TT
Cuts at: 1563 3708 4081 1563
Size: 2145 373 3063
Fragments arranged by size:
3063 2145 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2498 3644 5028 5296 1632
Size: 866 1146 1384 268 1917
Fragments arranged by size:
1917 1384 1146 866 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5384
AflIII A'CryG_T
Cuts at: 1203 4480 5457 1203
Size: 3277 977 1327
Fragments arranged by size:
3277 1327 977
AgeI A'CCGG_T
Cuts at: 4412 4412
Size: 5581
AhdI GACnn_n'nnGTC
Cuts at: 4310 4310
Size: 5581
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5581
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2328 1184
Size: 1144 4437
AlwNI CAG_nnn'CTG
Cuts at: 2687 3324 4896 2687
Size: 637 1572 3372
Fragments arranged by size:
3372 1572 637
ApaLI G'TGCA_C
Cuts at: 3638 4794 3638
Size: 1156 4425
ApoI r'AATT_y
Cuts at: 1493 2081 2834 2890 3116 3268 5377 1493
Size: 588 753 56 226 152 2109 1697
Fragments arranged by size:
2109 1697 753 588 226 152 56
AseI AT'TA_AT
Cuts at: 160 1032 4135 160
Size: 872 3103 1606
Fragments arranged by size:
3103 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5581
AvaI C'yCGr_G
Cuts at: 1870 1943 2906 1870
Size: 73 963 4545
Fragments arranged by size:
4545 963 73
BamHI G'GATC_C
Cuts at: 2911 3278 2911
Size: 367 5214
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2276 2902 4357 618
Size: 325 217 627 489 626 1455 1842
Fragments arranged by size:
1842 1455 627 626 489 325 217
BanII G_rGCy'C
Cuts at: 1391 2046 2182 2755 2900 1391
Size: 655 136 573 145 4072
Fragments arranged by size:
4072 655 573 145 136
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2292 928
Size: 971 393 4217
Fragments arranged by size:
4217 971 393
BbvCI CC'TCA_GC
Cuts at: 2257 2257
Size: 5581
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2122 2156 2485 2519 3772 3806 1903
Size: 34 185 34 329 34 1253 34 3678
Fragments arranged by size:
3678 1253 329 185 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2239 3473 4683 2239
Size: 1234 1210 3137
Fragments arranged by size:
3137 1234 1210
BclI T'GATC_A
Cuts at: 1548 2805 1548
Size: 1257 4324
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2678 4192 136
Size: 107 122 71 631 605 1006 1514 1525
Fragments arranged by size:
1525 1514 1006 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5576 5576
Size: 5581
BlpI GC'TnA_GC
Cuts at: 2998 2998
Size: 5581
Bme1580I G_kGCm'C
Cuts at: 1790 2731 3642 4798 1790
Size: 941 911 1156 2573
Fragments arranged by size:
2573 1156 941 911
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5581
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3312 4270 442
Size: 416 53 83 399 24 1895 958 1753
Fragments arranged by size:
1895 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5581
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2261 2753 4241 1239
Size: 1022 492 1488 2579
Fragments arranged by size:
2579 1488 1022 492
Bpu10I CC'TnA_GC
Cuts at: 2257 2257
Size: 5581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3706 4571 4869 3706
Size: 865 298 4418
Fragments arranged by size:
4418 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4244 882
Size: 617 2745 2219
Fragments arranged by size:
2745 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2446 3372 493
Size: 711 1242 926 2702
Fragments arranged by size:
2702 1242 926 711
BsaBI GATnn'nnATC
Cuts at: 5575 5575
Size: 5581
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3388 3770 4405 275
Size: 53 83 186 936 1855 382 635 1451
Fragments arranged by size:
1855 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4014 4412 4686 4833 5244 4014
Size: 398 274 147 411 4351
Fragments arranged by size:
4351 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2169 2199 1178
Size: 30 165 30 766 30 4560
Fragments arranged by size:
4560 766 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2018 2500 2578 1186
Size: 832 482 78 4189
Fragments arranged by size:
4189 832 482 78
BseYI C'CCAG_C
Cuts at: 2519 4784 5280 2519
Size: 2265 496 2820
Fragments arranged by size:
2820 2265 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5008
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3031 3792 4820 1540
Size: 275 186 1030 761 1028 2301
Fragments arranged by size:
2301 1030 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2755 3642 3727 4798 2755
Size: 887 85 1071 3538
Fragments arranged by size:
3538 1071 887 85
BsmI GAATG_Cn'
Cuts at: 3092 3185 3092
Size: 93 5488
BsmBI CGTCTCn'nnnn_
Cuts at: 2512 2512
Size: 5581
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2182 2731 2755 2900 3642 3727
Size: 399 256 136 549 24 145 742 85
Cuts at: 3727 4798 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 549 399 256 145 136
85 24
BspEI T'CCGG_A
Cuts at: 5244 5244
Size: 5581
BspHI T'CATG_A
Cuts at: 2392 3470 5200 2392
Size: 1078 1730 2773
Fragments arranged by size:
2773 1730 1078
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2936 844
Size: 2092 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3468 1394
Size: 429 1645 3507
Fragments arranged by size:
3507 1645 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2268 2494 4076 4250 65
Size: 1183 1020 226 1582 174 1396
Fragments arranged by size:
1582 1396 1183 1020 226 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4225 4412 1700
Size: 110 2415 187 2869
Fragments arranged by size:
2869 2415 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5581
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5581
BssSI C'ACGA_G
Cuts at: 3641 4653 3641
Size: 1012 4569
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5581
BstBI TT'CG_AA
Cuts at: 4385 4385
Size: 5581
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5581
BstYI r'GATC_y
Cuts at: 1972 2826 2911 3278 3663 3680 5132 5218 5230
Size: 854 85 367 385 17 1452 86 12
Cuts at: 5230 5576 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 854 385 367 346 86 85
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2280 513
Size: 552 939 276 3814
Fragments arranged by size:
3814 939 552 276
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4842
Fragments arranged by size:
4842 683 56
BtsI GCAGTG_nn'
Cuts at: 2233 3097 3891 3911 2233
Size: 864 794 20 3903
Fragments arranged by size:
3903 864 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5581
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5546
DraI TTT'AAA
Cuts at: 2885 2885
Size: 5581
DraIII CAC_nnn'GTG
Cuts at: 2410 2410
Size: 5581
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2200 4588 809
Size: 507 884 2388 1802
Fragments arranged by size:
2388 1802 884 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3028 3917 5546
Size: 54 1425 50 275 18 1198 889 1629
Cuts at: 5546 8
Size: 43
Fragments arranged by size:
1629 1425 1198 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3028 1537
Size: 275 1216 4090
Fragments arranged by size:
4090 1216 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2051 2140 2805 3511 1353
Size: 698 89 665 706 3423
Fragments arranged by size:
3423 706 698 665 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4168 4542 4688 387
Size: 1064 138 2579 374 146 1280
Fragments arranged by size:
2579 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2753 2753
Size: 5581
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2261 2498 2753 3644 4241 5028 5296
Size: 393 629 237 255 891 597 787 268
Cuts at: 5296 1239
Size: 1524
Fragments arranged by size:
1524 891 787 629 597 393 268 255
237
EcoRI G'AATT_C
Cuts at: 2081 2890 3268 2081
Size: 809 378 4394
Fragments arranged by size:
4394 809 378
FspI TGC'GCA
Cuts at: 2414 4087 2414
Size: 1673 3908
HaeII r_GCGC'y
Cuts at: 1678 4728 1678
Size: 3050 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2143 2175 2797 2829 4222
Size: 32 135 32 571 32 622 32 1393
Cuts at: 4222 4254 4296 4328 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1393 622 571 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2431 2925 3171 669
Size: 988 281 493 494 246 3079
Fragments arranged by size:
3079 988 494 493 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5581
HpaI GTT'AAC
Cuts at: 3171 3171
Size: 5581
KpnI G_GTAC'C
Cuts at: 2906 2906
Size: 5581
MfeI C'AATT_G
Cuts at: 3180 3180
Size: 5581
MluI A'CGCG_T
Cuts at: 5457 5457
Size: 5581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4695 4879 1305
Size: 3390 184 2007
Fragments arranged by size:
3390 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3759
Fragments arranged by size:
3759 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3539 3898 4057 518
Size: 648 2373 359 159 2042
Fragments arranged by size:
2373 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2997 3674 4822 5067 1606
Size: 1391 677 1148 245 2120
Fragments arranged by size:
2120 1391 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2280 513
Size: 552 939 276 3814
Fragments arranged by size:
3814 939 552 276
NdeI CA'TA_TG
Cuts at: 387 2700 387
Size: 2313 3268
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5581
NotI GC'GGCC_GC
Cuts at: 3028 3028
Size: 5581
NspI r_CATG'y
Cuts at: 1132 1229 2350 2939 4484 1132
Size: 97 1121 589 1545 2229
Fragments arranged by size:
2229 1545 1121 589 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5581
PciI A'CATG_T
Cuts at: 4480 4480
Size: 5581
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2188 2239 1108
Size: 1080 51 4450
Fragments arranged by size:
4450 1080 51
PfoI T'CCnGG_A
Cuts at: 2829 2829
Size: 5581
PmeI GTTT'AAAC
Cuts at: 2885 2885
Size: 5581
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3650 3682 5163 5195 3650
Size: 32 1481 32 4036
Fragments arranged by size:
4036 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5581
PsiI TTA'TAA
Cuts at: 3151 3151
Size: 5581
PstI C_TGCA'G
Cuts at: 830 2933 830
Size: 2103 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5581
SacI G_AGCT'C
Cuts at: 2755 2755
Size: 5581
SalI G'TCGA_C
Cuts at: 1936 2923 1936
Size: 987 4594
SapI GCTCTTCn'nnn_
Cuts at: 2051 2051
Size: 5581
SbfI CC_TGCA'GG
Cuts at: 2933 2933
Size: 5581
ScaI AGT'ACT
Cuts at: 3829 5541 3829
Size: 1712 3869
SfcI C'TryA_G
Cuts at: 826 1045 2216 2457 2929 4064 4745 4936 826
Size: 219 1171 241 472 1135 681 191 1471
Fragments arranged by size:
1471 1171 1135 681 472 241 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5581
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5581
SmaI CCC'GGG
Cuts at: 1872 2908 1872
Size: 1036 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3685 4586 4848 820
Size: 197 926 1742 901 262 1553
Fragments arranged by size:
1742 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3372 493
Size: 2879 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5581
SphI G_CATG'C
Cuts at: 1132 2939 1132
Size: 1807 3774
SspI AAT'ATT
Cuts at: 5 52 2606 3505 5
Size: 47 2554 899 2081
Fragments arranged by size:
2554 2081 899 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5581
StyI C'CwwG_G
Cuts at: 513 1065 2004 2101 2280 3020 513
Size: 552 939 97 179 740 3074
Fragments arranged by size:
3074 939 740 552 179 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3608 3625 3778 3963 4408 325
Size: 1255 2028 17 153 185 445 1498
Fragments arranged by size:
2028 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3827 5539 96
Size: 274 80 33 51 1443 1850 1712 138
Fragments arranged by size:
1850 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2097 2129 2169 2201 5541 5573 2097
Size: 32 40 32 3340 32 2105
Fragments arranged by size:
3340 2105 40 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5581
XbaI T'CTAG_A
Cuts at: 2917 2917
Size: 5581
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5581
XmaI C'CCGG_G
Cuts at: 1870 2906 1870
Size: 1036 4545
XmnI GAAnn'nnTTC
Cuts at: 3710 3710
Size: 5581
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3389 276
Size: 53 83 186 936 1855 2468
Fragments arranged by size:
2468 1855 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI