Restriction map for
pFN21AE1490
(Circular) (Six-base) MAPSORT of: pFN21AE1490.seq Check: 8841 from: 1 to: 6715
pFN21AE1490 6715 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2025 2025
Size: 6715
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4525 278
Size: 53 83 186 936 2989 2468
Fragments arranged by size:
2989 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3969 4058 1937
Size: 2032 89 4594
Fragments arranged by size:
4594 2032 89
Acc65I G'GTAC_C
Cuts at: 4036 4036
Size: 6715
AclI AA'CG_TT
Cuts at: 1563 4842 5215 1563
Size: 3279 373 3063
Fragments arranged by size:
3279 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2156 3187 3510 3713 4778 6162 6430 1632
Size: 524 1031 323 203 1065 1384 268 1917
Fragments arranged by size:
1917 1384 1065 1031 524 323 268 203
AfeI AGC'GCT
Cuts at: 2952 3094 3633 3933 2952
Size: 142 539 300 5734
Fragments arranged by size:
5734 539 300 142
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6518
AflIII A'CryG_T
Cuts at: 1203 2670 3803 5614 6591 1203
Size: 1467 1133 1811 977 1327
Fragments arranged by size:
1811 1467 1327 1133 977
AgeI A'CCGG_T
Cuts at: 5546 5546
Size: 6715
AhdI GACnn_n'nnGTC
Cuts at: 5444 5444
Size: 6715
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6715
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6715
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3258 3290 3258
Size: 32 6683
AlwNI CAG_nnn'CTG
Cuts at: 4458 6030 4458
Size: 1572 5143
ApaI G_GGCC'C
Cuts at: 3393 3393
Size: 6715
ApaLI G'TGCA_C
Cuts at: 4772 5928 4772
Size: 1156 5559
ApoI r'AATT_y
Cuts at: 1493 2942 4024 4250 4402 6511 1493
Size: 1449 1082 226 152 2109 1697
Fragments arranged by size:
2109 1697 1449 1082 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5269 160
Size: 872 4237 1606
Fragments arranged by size:
4237 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6715
AvaI C'yCGr_G
Cuts at: 1870 1943 2588 2608 2975 3011 3385 3827 4040
Size: 73 645 20 367 36 374 442 213
Cuts at: 4040 1870
Size: 4545
Fragments arranged by size:
4545 645 442 374 367 213 73 36
20
BamHI G'GATC_C
Cuts at: 3649 3831 4045 4412 3649
Size: 182 214 367 5952
Fragments arranged by size:
5952 367 214 182
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3213 4036 5491 618
Size: 325 217 627 1426 823 1455 1842
Fragments arranged by size:
1842 1455 1426 823 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2091 2904 3012 3090 3179 3393 3468 4034
Size: 700 813 108 78 89 214 75 566
Cuts at: 4034 1391
Size: 4072
Fragments arranged by size:
4072 813 700 566 214 108 89 78
75
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3498 928
Size: 971 1599 4145
Fragments arranged by size:
4145 1599 971
BbvCI CC'TCA_GC
Cuts at: 3701 3701
Size: 6715
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3392 3426 3808 3842 4906 4940 1903
Size: 34 1455 34 382 34 1064 34 3678
Fragments arranged by size:
3678 1455 1064 382 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4607 5817 4607
Size: 1210 5505
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6715
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2600 3388 3573
Size: 107 122 71 631 605 928 788 185
Cuts at: 3573 5326 136
Size: 1753 1525
Fragments arranged by size:
1753 1525 928 788 631 605 185 122
107 71
BglII A'GATC_T
Cuts at: 3436 3516 6710 3436
Size: 80 3194 3441
Fragments arranged by size:
3441 3194 80
BlpI GC'TnA_GC
Cuts at: 2159 3221 4132 2159
Size: 1062 911 4742
Fragments arranged by size:
4742 1062 911
Bme1580I G_kGCm'C
Cuts at: 1790 3393 3924 4776 5932 1790
Size: 1603 531 852 1156 2573
Fragments arranged by size:
2573 1603 1156 852 531
BmgBI CAC'GTC
Cuts at: 1318 3806 1318
Size: 2488 4227
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4446 5404 442
Size: 416 53 83 399 24 3029 958 1753
Fragments arranged by size:
3029 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6715
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3037 3037
Size: 6715
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2390 2785 3277 3348 3541 3872 3965 5375
Size: 1151 395 492 71 193 331 93 1410
Cuts at: 5375 1239
Size: 2579
Fragments arranged by size:
2579 1410 1151 492 395 331 193 93
71
Bpu10I CC'TnA_GC
Cuts at: 2154 2868 3084 3635 3701 2154
Size: 714 216 551 66 5168
Fragments arranged by size:
5168 714 551 216 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3086 4840 5705 6003 3086
Size: 1754 865 298 3798
Fragments arranged by size:
3798 1754 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3409 5378 882
Size: 617 1910 1969 2219
Fragments arranged by size:
2219 1969 1910 617
BsaAI yAC'GTr
Cuts at: 493 1204 3919 4506 493
Size: 711 2715 587 2702
Fragments arranged by size:
2715 2702 711 587
BsaBI GATnn'nnATC
Cuts at: 3782 6709 3782
Size: 2927 3788
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4522 4904 5539 275
Size: 53 83 186 936 2989 382 635 1451
Fragments arranged by size:
2989 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2049 3732 5148 5546 5820 5967 6378 2049
Size: 1683 1416 398 274 147 411 2386
Fragments arranged by size:
2386 1683 1416 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3258 3288 3872 3902 1178
Size: 30 165 30 1855 30 584 30 3991
Fragments arranged by size:
3991 1855 584 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2884 2992 3893 1186
Size: 1698 108 901 4008
Fragments arranged by size:
4008 1698 901 108
BseYI C'CCAG_C
Cuts at: 2090 2148 2404 2826 2980 3089 3158 3242 3308
Size: 58 256 422 154 109 69 84 66
Cuts at: 3308 3461 3563 3628 3654 5918 6414 2090
Size: 153 102 65 26 2264 496 2391
Fragments arranged by size:
2391 2264 496 422 256 154 153 109
102 84 69 66 65 58 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2220 1163
Size: 573 484 5658
Fragments arranged by size:
5658 573 484
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2936 2940 3192 3459 4165 4926
Size: 275 186 935 4 252 267 706 761
Cuts at: 4926 5954 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 935 761 706 275 267 252
186 4
BsiHKAI G_wGCw'C
Cuts at: 2169 3179 4776 4861 5932 2169
Size: 1010 1597 85 1071 2952
Fragments arranged by size:
2952 1597 1071 1010 85
BsmI GAATG_Cn'
Cuts at: 2564 4226 4319 2564
Size: 1662 93 4960
Fragments arranged by size:
4960 1662 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3941 3941
Size: 6715
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2169 2904 3012 3090 3179 3393
Size: 399 301 78 735 108 78 89 214
Cuts at: 3393 3468 3924 4034 4776 4861 5932 1391
Size: 75 456 110 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 735 456 399 301 214
110 108 89 85 78 78 75
BspEI T'CCGG_A
Cuts at: 2049 3732 6378 2049
Size: 1683 2646 2386
Fragments arranged by size:
2646 2386 1683
BspHI T'CATG_A
Cuts at: 4604 6334 4604
Size: 1730 4985
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2025 2106 3493 4070 844
Size: 1181 81 1387 577 3489
Fragments arranged by size:
3489 1387 1181 577 81
BsrBI CCG'CTC
Cuts at: 1394 1823 2753 3220 3453 4602 1394
Size: 429 930 467 233 1149 3507
Fragments arranged by size:
3507 1149 930 467 429 233
BsrDI GCAATG_nn'
Cuts at: 65 1248 3880 5210 5384 65
Size: 1183 2632 1330 174 1396
Fragments arranged by size:
2632 1396 1330 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2491 2594 2935 3197 5359 5546 1700
Size: 110 681 103 341 262 2162 187 2869
Fragments arranged by size:
2869 2162 681 341 262 187 110 103
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6715
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6715
BssSI C'ACGA_G
Cuts at: 4775 5787 4775
Size: 1012 5703
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6715
BstBI TT'CG_AA
Cuts at: 5519 5519
Size: 6715
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6715
BstXI CCAn_nnnn'nTGG
Cuts at: 3352 3352
Size: 6715
BstYI r'GATC_y
Cuts at: 1972 3436 3516 3649 3831 3846 4045 4412 4797
Size: 1464 80 133 182 15 199 367 385
Cuts at: 4797 4814 6266 6352 6364 6710 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1464 1452 385 367 346 199 182
133 86 80 17 15 12
Bsu36I CC'TnA_GG
Cuts at: 2226 2262 2298 2334 2442 2697 2226
Size: 36 36 36 108 255 6244
Fragments arranged by size:
6244 255 108 36 36 36
BtgI C'CryG_G
Cuts at: 513 1065 2004 2028 2930 3192 3393 3675 513
Size: 552 939 24 902 262 201 282 3553
Fragments arranged by size:
3553 939 902 552 282 262 201 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5976
Fragments arranged by size:
5976 683 56
BtsI GCAGTG_nn'
Cuts at: 2208 3179 4231 5025 5045 2208
Size: 971 1052 794 20 3878
Fragments arranged by size:
3878 1052 971 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6715
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3648 3683 529
Size: 35 3084 35 3561
Fragments arranged by size:
3561 3084 35 35
DraI TTT'AAA
Cuts at: 4019 4019
Size: 6715
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5722 809
Size: 507 4406 1802
Fragments arranged by size:
4406 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2933 2937 3189
Size: 54 1425 50 275 18 1103 4 252
Cuts at: 3189 3195 3456 4162 5051 6680 8
Size: 6 261 706 889 1629 43
Fragments arranged by size:
1629 1425 1103 889 706 275 261 252
54 50 43 18 6 4
EagI C'GGCC_G
Cuts at: 1537 1812 2933 2937 3189 3456 4162 1537
Size: 275 1121 4 252 267 706 4090
Fragments arranged by size:
4090 1121 706 275 267 252 4
EarI CTCTTCn'nnn_
Cuts at: 1353 4645 1353
Size: 3292 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3340 5302 5676 5822 387
Size: 1064 138 1751 1962 374 146 1280
Fragments arranged by size:
1962 1751 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3177 3177
Size: 6715
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2390 2785 3187 3277 3348 3510
Size: 393 524 234 395 402 90 71 162
Cuts at: 3510 3541 3713 3872 3965 4778 5375 6162 6430
Size: 31 172 159 93 813 597 787 268
Cuts at: 6430 1239
Size: 1524
Fragments arranged by size:
1524 813 787 597 524 402 395 393
268 234 172 162 159 93 90 71
31
EcoO109I rG'GnC_Cy
Cuts at: 2179 2455 2613 2878 2889 2997 3389 3447 3505
Size: 276 158 265 11 108 392 58 58
Cuts at: 3505 3895 2179
Size: 390 4999
Fragments arranged by size:
4999 392 390 276 265 158 108 58
58 11
EcoRI G'AATT_C
Cuts at: 2942 4024 4402 2942
Size: 1082 378 5255
Fragments arranged by size:
5255 1082 378
FseI GG_CCGG'CC
Cuts at: 2939 2939
Size: 6715
FspI TGC'GCA
Cuts at: 2655 5221 2655
Size: 2566 4149
HaeII r_GCGC'y
Cuts at: 1678 2808 2954 3055 3096 3590 3635 3935 5862
Size: 1130 146 101 41 494 45 300 1927
Cuts at: 5862 1678
Size: 2531
Fragments arranged by size:
2531 1927 1130 494 300 146 101 45
41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5356 5388 5430 5462 1373
Size: 32 135 32 3784 32 42 32 2626
Fragments arranged by size:
3784 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3970 4059 4305 669
Size: 988 281 2032 89 246 3079
Fragments arranged by size:
3079 2032 988 281 246 89
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6715
HpaI GTT'AAC
Cuts at: 4305 4305
Size: 6715
KpnI G_GTAC'C
Cuts at: 4040 4040
Size: 6715
MfeI C'AATT_G
Cuts at: 4314 4314
Size: 6715
MluI A'CGCG_T
Cuts at: 6591 6591
Size: 6715
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5829 6013 1305
Size: 4524 184 2007
Fragments arranged by size:
4524 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4893
Fragments arranged by size:
4893 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3776 4673 5032 5191 518
Size: 648 2610 897 359 159 2042
Fragments arranged by size:
2610 2042 897 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2185 2404 2836 2932 3242 3255 3379 3461
Size: 579 219 432 96 310 13 124 82
Cuts at: 3461 3700 4131 4808 5956 6201 1606
Size: 239 431 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 579 432 431 310 245
239 219 124 96 82 13
NaeI GCC'GGC
Cuts at: 2493 2937 3199 2493
Size: 444 262 6009
Fragments arranged by size:
6009 444 262
NcoI C'CATG_G
Cuts at: 513 1065 2004 2028 3675 513
Size: 552 939 24 1647 3553
Fragments arranged by size:
3553 1647 939 552 24
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6715
NgoMIV G'CCGG_C
Cuts at: 2491 2935 3197 2491
Size: 444 262 6009
Fragments arranged by size:
6009 444 262
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6715
NotI GC'GGCC_GC
Cuts at: 4162 4162
Size: 6715
NspI r_CATG'y
Cuts at: 1132 1229 4073 5618 1132
Size: 97 2844 1545 2229
Fragments arranged by size:
2844 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3227 4006 1348
Size: 1879 779 4057
Fragments arranged by size:
4057 1879 779
PciI A'CATG_T
Cuts at: 5614 5614
Size: 6715
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6715
PfoI T'CCnGG_A
Cuts at: 3474 3849 3474
Size: 375 6340
PmeI GTTT'AAAC
Cuts at: 4019 4019
Size: 6715
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2999 3031 3258 3290 3713 3745 4784 4816 6297
Size: 32 227 32 423 32 1039 32 1481
Cuts at: 6297 6329 2999
Size: 32 3385
Fragments arranged by size:
3385 1481 1039 423 227 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2613 2878 2889 2997 3447 3505 3895 2613
Size: 265 11 108 450 58 390 5433
Fragments arranged by size:
5433 450 390 265 108 58 11
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6715
PsiI TTA'TAA
Cuts at: 4285 4285
Size: 6715
PspOMI G'GGCC_C
Cuts at: 3389 3389
Size: 6715
PstI C_TGCA'G
Cuts at: 830 2026 2439 2511 3045 3427 4067 830
Size: 1196 413 72 534 382 640 3478
Fragments arranged by size:
3478 1196 640 534 413 382 72
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6715
PvuII CAG'CTG
Cuts at: 2404 3700 2404
Size: 1296 5419
SacI G_AGCT'C
Cuts at: 3179 3179
Size: 6715
SacII CC_GC'GG
Cuts at: 2933 2933
Size: 6715
SalI G'TCGA_C
Cuts at: 1936 3968 4057 1936
Size: 2032 89 4594
Fragments arranged by size:
4594 2032 89
SanDI GG'GwC_CC
Cuts at: 3895 3895
Size: 6715
SbfI CC_TGCA'GG
Cuts at: 4067 4067
Size: 6715
ScaI AGT'ACT
Cuts at: 4963 6675 4963
Size: 1712 5003
SfcI C'TryA_G
Cuts at: 826 1045 2022 2435 2507 3041 3423 4063 5198
Size: 219 977 413 72 534 382 640 1135
Cuts at: 5198 5879 6070 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 977 681 640 534 413 382
219 191 72
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6715
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6715
SmaI CCC'GGG
Cuts at: 1872 3387 4042 1872
Size: 1515 655 4545
Fragments arranged by size:
4545 1515 655
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3101 4819 5720 5982 820
Size: 197 926 1158 1718 901 262 1553
Fragments arranged by size:
1718 1553 1158 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4506 493
Size: 4013 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6715
SphI G_CATG'C
Cuts at: 1132 4073 1132
Size: 2941 3774
SspI AAT'ATT
Cuts at: 5 52 4639 5
Size: 47 4587 2081
Fragments arranged by size:
4587 2081 47
StuI AGG'CCT
Cuts at: 1517 2075 2468 2822 3270 3300 3331 1517
Size: 558 393 354 448 30 31 4901
Fragments arranged by size:
4901 558 448 393 354 31 30
StyI C'CwwG_G
Cuts at: 513 1065 2004 2028 2423 3570 3675 4154 513
Size: 552 939 24 395 1147 105 479 3074
Fragments arranged by size:
3074 1147 939 552 479 395 105 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3495 3688 4742 4759 4912 5097 5542
Size: 1255 1915 193 1054 17 153 185 445
Cuts at: 5542 325
Size: 1498
Fragments arranged by size:
1915 1498 1255 1054 445 193 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4961 6673 96
Size: 274 80 33 51 1443 2984 1712 138
Fragments arranged by size:
2984 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2404 2436 3872 3904 6675 6707 2404
Size: 32 1436 32 2771 32 2412
Fragments arranged by size:
2771 2412 1436 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6715
XbaI T'CTAG_A
Cuts at: 4051 4051
Size: 6715
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6715
XmaI C'CCGG_G
Cuts at: 1870 3385 4040 1870
Size: 1515 655 4545
Fragments arranged by size:
4545 1515 655
XmnI GAAnn'nnTTC
Cuts at: 2141 3740 4844 2141
Size: 1599 1104 4012
Fragments arranged by size:
4012 1599 1104
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4523 276
Size: 53 83 186 936 2989 2468
Fragments arranged by size:
2989 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII
EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI
PacI PmlI PspXI PsrI RsrII SapI SexAI SfiI
SfoI SrfI SwaI XcmI