Restriction map for
pFN21AE1489
(Circular) (Six-base) MAPSORT of: pFN21AE1489.seq Check: 803 from: 1 to: 6070
pFN21AE1489 6070 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2513 2513
Size: 6070
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3880 278
Size: 53 83 186 936 2344 2468
Fragments arranged by size:
2468 2344 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3413 1937
Size: 1476 4594
Acc65I G'GTAC_C
Cuts at: 3391 3391
Size: 6070
AclI AA'CG_TT
Cuts at: 1563 4197 4570 1563
Size: 2634 373 3063
Fragments arranged by size:
3063 2634 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2262 2806 2842 3002 4133 5517 5785 1632
Size: 630 544 36 160 1131 1384 268 1917
Fragments arranged by size:
1917 1384 1131 630 544 268 160 36
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5873
AflIII A'CryG_T
Cuts at: 1203 3271 4969 5946 1203
Size: 2068 1698 977 1327
Fragments arranged by size:
2068 1698 1327 977
AgeI A'CCGG_T
Cuts at: 4901 4901
Size: 6070
AhdI GACnn_n'nnGTC
Cuts at: 4799 4799
Size: 6070
AleI CACnn'nnGTG
Cuts at: 1166 2636 1166
Size: 1470 4600
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2654 1184
Size: 1470 4600
AlwNI CAG_nnn'CTG
Cuts at: 2298 2448 3813 5385 2298
Size: 150 1365 1572 2983
Fragments arranged by size:
2983 1572 1365 150
ApaLI G'TGCA_C
Cuts at: 2465 2500 2575 4127 5283 2465
Size: 35 75 1552 1156 3252
Fragments arranged by size:
3252 1552 1156 75 35
ApoI r'AATT_y
Cuts at: 1493 3122 3379 3605 3757 5866 1493
Size: 1629 257 226 152 2109 1697
Fragments arranged by size:
2109 1697 1629 257 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4624 160
Size: 872 3592 1606
Fragments arranged by size:
3592 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
AvaI C'yCGr_G
Cuts at: 1870 1943 2227 3395 1870
Size: 73 284 1168 4545
Fragments arranged by size:
4545 1168 284 73
AvrII C'CTAG_G
Cuts at: 2104 3136 2104
Size: 1032 5038
BamHI G'GATC_C
Cuts at: 3400 3767 3400
Size: 367 5703
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2630 2947 3132 3391 4846
Size: 325 217 627 843 317 185 259 1455
Cuts at: 4846 618
Size: 1842
Fragments arranged by size:
1842 1455 843 627 325 317 259 217
185
BanII G_rGCy'C
Cuts at: 1391 3389 1391
Size: 1998 4072
BbeI G_GCGC'C
Cuts at: 3136 3136
Size: 6070
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3353 928
Size: 971 1454 3645
Fragments arranged by size:
3645 1454 971
BbvCI CC'TCA_GC
Cuts at: 2303 2303
Size: 6070
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4261 4295 1903
Size: 34 2324 34 3678
Fragments arranged by size:
3678 2324 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2230 3962 5172 2230
Size: 1732 1210 3128
Fragments arranged by size:
3128 1732 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6070
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4681 136
Size: 107 122 71 631 605 3009 1525
Fragments arranged by size:
3009 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2343 6065 2343
Size: 3722 2348
BlpI GC'TnA_GC
Cuts at: 3143 3487 3143
Size: 344 5726
Bme1580I G_kGCm'C
Cuts at: 1790 2469 2504 2579 2618 2871 3105 4131 5287
Size: 679 35 75 39 253 234 1026 1156
Cuts at: 5287 1790
Size: 2573
Fragments arranged by size:
2573 1156 1026 679 253 234 75 39
35
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6070
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2865 3351 3801
Size: 416 53 83 399 24 1448 486 450
Cuts at: 3801 4759 442
Size: 958 1753
Fragments arranged by size:
1753 1448 958 486 450 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6070
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2281 2560 2281
Size: 279 5791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2072 2591 2817 2872 3188 3244 3278 3326
Size: 833 519 226 55 316 56 34 48
Cuts at: 3326 4730 1239
Size: 1404 2579
Fragments arranged by size:
2579 1404 833 519 316 226 56 55
48 34
Bpu10I CC'TnA_GC
Cuts at: 2303 2303
Size: 6070
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4195 5060 5358 4195
Size: 865 298 4907
Fragments arranged by size:
4907 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4733 882
Size: 617 3234 2219
Fragments arranged by size:
3234 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3861 493
Size: 711 2657 2702
Fragments arranged by size:
2702 2657 711
BsaBI GATnn'nnATC
Cuts at: 6064 6064
Size: 6070
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3133 3877 4259 4894
Size: 53 83 186 936 1600 744 382 635
Cuts at: 4894 275
Size: 1451
Fragments arranged by size:
1600 1451 936 744 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4503 4901 5175 5322 5733 4503
Size: 398 274 147 411 4840
Fragments arranged by size:
4840 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2031 2061 3261 3291 1178
Size: 30 165 30 628 30 1200 30 3957
Fragments arranged by size:
3957 1200 628 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2675 3332 1186
Size: 1489 657 3924
Fragments arranged by size:
3924 1489 657
BseYI C'CCAG_C
Cuts at: 3265 3295 5273 5769 3265
Size: 30 1978 496 3566
Fragments arranged by size:
3566 1978 496 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5497
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3163 3520 4281 5309 1540
Size: 275 186 1162 357 761 1028 2301
Fragments arranged by size:
2301 1162 1028 761 357 275 186
BsiHKAI G_wGCw'C
Cuts at: 2049 2469 2504 2579 3279 4131 4216 5287 2049
Size: 420 35 75 700 852 85 1071 2832
Fragments arranged by size:
2832 1071 852 700 420 85 75 35
BsmI GAATG_Cn'
Cuts at: 3581 3674 3581
Size: 93 5977
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2469 2504 2579 2618 2871 3105
Size: 399 259 420 35 75 39 253 234
Cuts at: 3105 3279 3389 4131 4216 5287 1391
Size: 174 110 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 420 399 259 253 234
174 110 85 75 39 35
BspEI T'CCGG_A
Cuts at: 5733 5733
Size: 6070
BspHI T'CATG_A
Cuts at: 3959 5689 3959
Size: 1730 4340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2037 2504 2513 3300 3425 844
Size: 1193 467 9 787 125 3489
Fragments arranged by size:
3489 1193 787 467 125 9
BsrBI CCG'CTC
Cuts at: 1394 1823 2262 3957 1394
Size: 429 439 1695 3507
Fragments arranged by size:
3507 1695 439 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4565 4739 65
Size: 1183 3317 174 1396
Fragments arranged by size:
3317 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2400 4714 4901 1700
Size: 110 590 2314 187 2869
Fragments arranged by size:
2869 2314 590 187 110
BsrGI T'GTAC_A
Cuts at: 96 2208 96
Size: 2112 3958
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6070
BssSI C'ACGA_G
Cuts at: 3152 4130 5142 3152
Size: 978 1012 4080
Fragments arranged by size:
4080 1012 978
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2448 794
Size: 1654 4416
BstBI TT'CG_AA
Cuts at: 4874 4874
Size: 6070
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6070
BstYI r'GATC_y
Cuts at: 1972 2343 3400 3767 4152 4169 5621 5707 5719
Size: 371 1057 367 385 17 1452 86 12
Cuts at: 5719 6065 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1057 385 371 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2077 2077
Size: 6070
BtgI C'CryG_G
Cuts at: 513 1065 2004 3087 3239 513
Size: 552 939 1083 152 3344
Fragments arranged by size:
3344 1083 939 552 152
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5331
Fragments arranged by size:
5331 683 56
BtsI GCAGTG_nn'
Cuts at: 2296 3586 4380 4400 2296
Size: 1290 794 20 3966
Fragments arranged by size:
3966 1290 794 20
ClaI AT'CG_AT
Cuts at: 1554 3159 1554
Size: 1605 4465
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6035
DraI TTT'AAA
Cuts at: 3374 3374
Size: 6070
DraIII CAC_nnn'GTG
Cuts at: 2376 2499 2376
Size: 123 5947
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5077 809
Size: 507 3761 1802
Fragments arranged by size:
3761 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3517 4406 6035
Size: 54 1425 50 275 18 1687 889 1629
Cuts at: 6035 8
Size: 43
Fragments arranged by size:
1687 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3517 1537
Size: 275 1705 4090
Fragments arranged by size:
4090 1705 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2114 2353 2693 4000 1353
Size: 761 239 340 1307 3423
Fragments arranged by size:
3423 1307 761 340 239
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4657 5031 5177 387
Size: 1064 138 3068 374 146 1280
Fragments arranged by size:
3068 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2262 2591 2806 2817 2842 2872
Size: 393 440 190 329 215 11 25 30
Cuts at: 2872 3002 3188 3244 3278 3326 4133 4730 5517
Size: 130 186 56 34 48 807 597 787
Cuts at: 5517 5785 1239
Size: 268 1524
Fragments arranged by size:
1524 807 787 597 440 393 329 268
215 190 186 130 56 48 34 30
25 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2103 3194 2103
Size: 1091 4979
EcoRI G'AATT_C
Cuts at: 3122 3379 3757 3122
Size: 257 378 5435
Fragments arranged by size:
5435 378 257
FspI TGC'GCA
Cuts at: 4576 4576
Size: 6070
HaeII r_GCGC'y
Cuts at: 1678 3039 3136 3187 3326 5217 1678
Size: 1361 97 51 139 1891 2531
Fragments arranged by size:
2531 1891 1361 139 97 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2286 2318 3261 3293 4711
Size: 32 135 32 714 32 943 32 1418
Cuts at: 4711 4743 4785 4817 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1418 943 714 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3414 3660 669
Size: 988 281 1476 246 3079
Fragments arranged by size:
3079 1476 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2818 748
Size: 2070 4000
HpaI GTT'AAC
Cuts at: 3660 3660
Size: 6070
KasI G'GCGC_C
Cuts at: 3132 3132
Size: 6070
KpnI G_GTAC'C
Cuts at: 3395 3395
Size: 6070
MfeI C'AATT_G
Cuts at: 2437 3669 2437
Size: 1232 4838
MluI A'CGCG_T
Cuts at: 3271 5946 3271
Size: 2675 3395
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3021 5184 5368 1305
Size: 1716 2163 184 2007
Fragments arranged by size:
2163 2007 1716 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4248
Fragments arranged by size:
4248 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2636 4028 4387 4546 518
Size: 648 1470 1392 359 159 2042
Fragments arranged by size:
2042 1470 1392 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2307 3241 3486 4163 5311 5556 1606
Size: 701 934 245 677 1148 245 2120
Fragments arranged by size:
2120 1148 934 701 677 245 245
NarI GG'CG_CC
Cuts at: 3133 3133
Size: 6070
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4579
Fragments arranged by size:
4579 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6070
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6070
NotI GC'GGCC_GC
Cuts at: 3517 3517
Size: 6070
NspI r_CATG'y
Cuts at: 1132 1229 3214 3428 4973 1132
Size: 97 1985 214 1545 2229
Fragments arranged by size:
2229 1985 1545 214 97
PasI CC'CwG_GG
Cuts at: 1348 2851 1348
Size: 1503 4567
PciI A'CATG_T
Cuts at: 4969 4969
Size: 6070
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6070
PfoI T'CCnGG_A
Cuts at: 2049 2049
Size: 6070
PmeI GTTT'AAAC
Cuts at: 3374 3374
Size: 6070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4139 4171 5652 5684 4139
Size: 32 1481 32 4525
Fragments arranged by size:
4525 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6070
PsiI TTA'TAA
Cuts at: 3640 3640
Size: 6070
PstI C_TGCA'G
Cuts at: 830 2249 2387 3177 3422 830
Size: 1419 138 790 245 3478
Fragments arranged by size:
3478 1419 790 245 138
PvuI CG_AT'CG
Cuts at: 2001 3163 2001
Size: 1162 4908
PvuII CAG'CTG
Cuts at: 2307 2307
Size: 6070
SacII CC_GC'GG
Cuts at: 3242 3242
Size: 6070
SalI G'TCGA_C
Cuts at: 1936 3412 1936
Size: 1476 4594
SbfI CC_TGCA'GG
Cuts at: 3422 3422
Size: 6070
ScaI AGT'ACT
Cuts at: 4318 6030 4318
Size: 1712 4358
SfcI C'TryA_G
Cuts at: 826 1045 2245 2383 2548 3173 3418 4553 5234
Size: 219 1200 138 165 625 245 1135 681
Cuts at: 5234 5425 826
Size: 191 1471
Fragments arranged by size:
1471 1200 1135 681 625 245 219 191
165 138
SfoI GGC'GCC
Cuts at: 3134 3134
Size: 6070
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6070
SmaI CCC'GGG
Cuts at: 1872 2229 3397 1872
Size: 357 1168 4545
Fragments arranged by size:
4545 1168 357
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4174 5075 5337 820
Size: 197 926 2231 901 262 1553
Fragments arranged by size:
2231 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3861 493
Size: 3368 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6070
SphI G_CATG'C
Cuts at: 1132 3214 3428 1132
Size: 2082 214 3774
Fragments arranged by size:
3774 2082 214
SspI AAT'ATT
Cuts at: 5 52 3994 5
Size: 47 3942 2081
Fragments arranged by size:
3942 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6070
StyI C'CwwG_G
Cuts at: 513 1065 2004 2104 2140 3136 3509 513
Size: 552 939 100 36 996 373 3074
Fragments arranged by size:
3074 996 939 552 373 100 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4097 4114 4267 4452 4897 325
Size: 1255 2517 17 153 185 445 1498
Fragments arranged by size:
2517 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2208 4316 6028
Size: 274 80 33 51 1443 231 2108 1712
Cuts at: 6028 96
Size: 138
Fragments arranged by size:
2108 1712 1443 274 231 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2031 2063 2360 2392 6030 6062 2031
Size: 32 297 32 3638 32 2039
Fragments arranged by size:
3638 2039 297 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6070
XbaI T'CTAG_A
Cuts at: 3406 3406
Size: 6070
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6070
XmaI C'CCGG_G
Cuts at: 1870 2227 3395 1870
Size: 357 1168 4545
Fragments arranged by size:
4545 1168 357
XmnI GAAnn'nnTTC
Cuts at: 3122 4199 3122
Size: 1077 4993
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3878 276
Size: 53 83 186 936 2344 2468
Fragments arranged by size:
2468 2344 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BfrBI BsiWI BsmBI
BstXI BstZ17I EcoICRI EcoO109I EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI
SrfI SwaI XcmI