Restriction map for
pFN21AE1476
(Circular) (Six-base) MAPSORT of: pFN21AE1476.seq Check: 5356 from: 1 to: 6670
pFN21AE1476 6670 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4480 278
Size: 53 83 186 936 2944 2468
Fragments arranged by size:
2944 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4013 1937
Size: 2076 4594
Acc65I G'GTAC_C
Cuts at: 2525 3991 2525
Size: 1466 5204
AclI AA'CG_TT
Cuts at: 1563 4797 5170 1563
Size: 3234 373 3063
Fragments arranged by size:
3234 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2873 2954 3206 3281 4733 6117 6385 1632
Size: 1241 81 252 75 1452 1384 268 1917
Fragments arranged by size:
1917 1452 1384 1241 268 252 81 75
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6473
AflIII A'CryG_T
Cuts at: 1203 5569 6546 1203
Size: 4366 977 1327
Fragments arranged by size:
4366 1327 977
AgeI A'CCGG_T
Cuts at: 5501 5501
Size: 6670
AhdI GACnn_n'nnGTC
Cuts at: 5399 5399
Size: 6670
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6670
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6670
AlwNI CAG_nnn'CTG
Cuts at: 2492 2593 2831 4413 5985 2492
Size: 101 238 1582 1572 3177
Fragments arranged by size:
3177 1582 1572 238 101
ApaI G_GGCC'C
Cuts at: 2996 3147 3416 2996
Size: 151 269 6250
Fragments arranged by size:
6250 269 151
ApaLI G'TGCA_C
Cuts at: 4727 5883 4727
Size: 1156 5514
ApoI r'AATT_y
Cuts at: 1493 3598 3979 4205 4357 6466 1493
Size: 2105 381 226 152 2109 1697
Fragments arranged by size:
2109 2105 1697 381 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5224 160
Size: 872 4192 1606
Fragments arranged by size:
4192 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6670
AvaI C'yCGr_G
Cuts at: 1870 1943 2520 3124 3416 3562 3995 1870
Size: 73 577 604 292 146 433 4545
Fragments arranged by size:
4545 604 577 433 292 146 73
BamHI G'GATC_C
Cuts at: 3154 4000 4367 3154
Size: 846 367 5457
Fragments arranged by size:
5457 846 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2525 3991 5446 618
Size: 325 217 627 738 1466 1455 1842
Fragments arranged by size:
1842 1466 1455 738 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2090 2996 3147 3416 3548 3569 3672 3989
Size: 699 906 151 269 132 21 103 317
Cuts at: 3989 1391
Size: 4072
Fragments arranged by size:
4072 906 699 317 269 151 132 103
21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2868 3507 928
Size: 971 969 639 4091
Fragments arranged by size:
4091 971 969 639
BbvCI CC'TCA_GC
Cuts at: 3867 3867
Size: 6670
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2017 2035 2051 2069 2501 2535 4861
Size: 34 80 18 16 18 432 34 2326
Cuts at: 4861 4895 1903
Size: 34 3678
Fragments arranged by size:
3678 2326 432 80 34 34 34 18
18 16
BciVI GTATCCnnnnn_n'
Cuts at: 3122 4562 5772 3122
Size: 1440 1210 4020
Fragments arranged by size:
4020 1440 1210
BclI T'GATC_A
Cuts at: 1548 2601 1548
Size: 1053 5617
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2409 5281 136
Size: 107 122 71 631 605 737 2872 1525
Fragments arranged by size:
2872 1525 737 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6665 6665
Size: 6670
BlpI GC'TnA_GC
Cuts at: 2127 2845 3557 3713 4087 2127
Size: 718 712 156 374 4710
Fragments arranged by size:
4710 718 712 374 156
Bme1580I G_kGCm'C
Cuts at: 1790 2440 2996 3147 3416 4731 5887 1790
Size: 650 556 151 269 1315 1156 2573
Fragments arranged by size:
2573 1315 1156 650 556 269 151
BmgBI CAC'GTC
Cuts at: 1318 2866 1318
Size: 1548 5122
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2970 3153 4401
Size: 416 53 83 399 24 1553 183 1248
Cuts at: 4401 5359 442
Size: 958 1753
Fragments arranged by size:
1753 1553 1248 958 416 399 183 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6670
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2641 5330 1239
Size: 1402 2689 2579
Fragments arranged by size:
2689 2579 1402
Bpu10I CC'TnA_GC
Cuts at: 2111 2703 3867 2111
Size: 592 1164 4914
Fragments arranged by size:
4914 1164 592
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3614 4795 5660 5958 3614
Size: 1181 865 298 4326
Fragments arranged by size:
4326 1181 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5333 882
Size: 617 3834 2219
Fragments arranged by size:
3834 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4461 493
Size: 711 3257 2702
Fragments arranged by size:
3257 2702 711
BsaBI GATnn'nnATC
Cuts at: 3303 6664 3303
Size: 3361 3309
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4477 4859 5494 275
Size: 53 83 186 936 2944 382 635 1451
Fragments arranged by size:
2944 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3114 3459 5103 5501 5775 5922 6333 3114
Size: 345 1644 398 274 147 411 3451
Fragments arranged by size:
3451 1644 411 398 345 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3519 3549 1178
Size: 30 165 30 2116 30 4299
Fragments arranged by size:
4299 2116 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3694 3848 1186
Size: 2508 154 4008
Fragments arranged by size:
4008 2508 154
BseYI C'CCAG_C
Cuts at: 3432 3510 3548 3575 5873 6369 3432
Size: 78 38 27 2298 496 3733
Fragments arranged by size:
3733 2298 496 78 38 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3127 1163
Size: 573 1391 4706
Fragments arranged by size:
4706 1391 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4120 4881 5909 1540
Size: 275 186 2119 761 1028 2301
Fragments arranged by size:
2301 2119 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2521 2709 4731 4816 5887 2521
Size: 188 2022 85 1071 3304
Fragments arranged by size:
3304 2022 1071 188 85
BsmI GAATG_Cn'
Cuts at: 2399 4181 4274 2399
Size: 1782 93 4795
Fragments arranged by size:
4795 1782 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2440 2521 2709 2996 3147 3416
Size: 399 300 350 81 188 287 151 269
Cuts at: 3416 3548 3569 3672 3989 4731 4816 5887 1391
Size: 132 21 103 317 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 350 317 300 287
269 188 151 132 103 85 81 21
BspEI T'CCGG_A
Cuts at: 3114 6333 3114
Size: 3219 3451
BspHI T'CATG_A
Cuts at: 4559 6289 4559
Size: 1730 4940
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3757 3972 4025 844
Size: 2913 215 53 3489
Fragments arranged by size:
3489 2913 215 53
BsrBI CCG'CTC
Cuts at: 1394 1823 3376 3605 4557 1394
Size: 429 1553 229 952 3507
Fragments arranged by size:
3507 1553 952 429 229
BsrDI GCAATG_nn'
Cuts at: 65 1248 3244 3325 5165 5339 65
Size: 1183 1996 81 1840 174 1396
Fragments arranged by size:
1996 1840 1396 1183 174 81
BsrFI r'CCGG_y
Cuts at: 1700 1810 2186 3882 5314 5501 1700
Size: 110 376 1696 1432 187 2869
Fragments arranged by size:
2869 1696 1432 376 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6670
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6670
BssSI C'ACGA_G
Cuts at: 3371 4730 5742 3371
Size: 1359 1012 4299
Fragments arranged by size:
4299 1359 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2274 794
Size: 1480 5190
BstBI TT'CG_AA
Cuts at: 3891 5474 3891
Size: 1583 5087
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6670
BstXI CCAn_nnnn'nTGG
Cuts at: 2346 3514 2346
Size: 1168 5502
BstYI r'GATC_y
Cuts at: 1972 3154 3225 4000 4367 4752 4769 6221 6307
Size: 1182 71 775 367 385 17 1452 86
Cuts at: 6307 6319 6665 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1182 775 385 367 346 86
71 17 12
Bsu36I CC'TnA_GG
Cuts at: 3831 3831
Size: 6670
BtgI C'CryG_G
Cuts at: 513 1065 2004 2345 2949 3244 513
Size: 552 939 341 604 295 3939
Fragments arranged by size:
3939 939 604 552 341 295
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2038 487
Size: 683 56 812 5119
Fragments arranged by size:
5119 812 683 56
BtsI GCAGTG_nn'
Cuts at: 2519 4186 4980 5000 2519
Size: 1667 794 20 4189
Fragments arranged by size:
4189 1667 794 20
ClaI AT'CG_AT
Cuts at: 1554 3252 1554
Size: 1698 4972
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2617 2652 529
Size: 35 2053 35 4547
Fragments arranged by size:
4547 2053 35 35
DraI TTT'AAA
Cuts at: 3974 3974
Size: 6670
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5677 809
Size: 507 4361 1802
Fragments arranged by size:
4361 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2188 2348 3241
Size: 54 1425 50 275 18 358 160 893
Cuts at: 3241 4117 5006 6635 8
Size: 876 889 1629 43
Fragments arranged by size:
1629 1425 893 889 876 358 275 160
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4117 1537
Size: 275 2305 4090
Fragments arranged by size:
4090 2305 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2782 3846 4600 1353
Size: 1429 1064 754 3423
Fragments arranged by size:
3423 1429 1064 754
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5257 5631 5777 387
Size: 1064 138 3668 374 146 1280
Fragments arranged by size:
3668 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2641 2873 2954 3206 3281 4733 5330
Size: 393 1009 232 81 252 75 1452 597
Cuts at: 5330 6117 6385 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1452 1009 787 597 393 268 252
232 81 75
EcoNI CCTnn'n_nnAGG
Cuts at: 2293 2293
Size: 6670
EcoO109I rG'GnC_Cy
Cuts at: 2010 2286 2912 2992 2993 3143 3144 3412 3413
Size: 276 626 80 1 150 1 268 1
Cuts at: 3413 2010
Size: 5267
Fragments arranged by size:
5267 626 276 268 150 80 1 1
1
EcoRI G'AATT_C
Cuts at: 3598 3979 4357 3598
Size: 381 378 5911
Fragments arranged by size:
5911 381 378
EcoRV GAT'ATC
Cuts at: 3256 3301 3256
Size: 45 6625
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3667 3667
Size: 6670
FspI TGC'GCA
Cuts at: 5176 5176
Size: 6670
HaeII r_GCGC'y
Cuts at: 1678 3447 5817 1678
Size: 1769 2370 2531
Fragments arranged by size:
2531 2370 1769
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2992 3024 3677 3709 5311
Size: 32 135 32 1420 32 653 32 1602
Cuts at: 5311 5343 5385 5417 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1602 1420 653 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2176 2899 4014 4260 669
Size: 988 281 238 723 1115 246 3079
Fragments arranged by size:
3079 1115 988 723 281 246 238
HindIII A'AGCT_T
Cuts at: 748 3263 748
Size: 2515 4155
HpaI GTT'AAC
Cuts at: 4260 4260
Size: 6670
KpnI G_GTAC'C
Cuts at: 2529 3995 2529
Size: 1466 5204
MfeI C'AATT_G
Cuts at: 4269 4269
Size: 6670
MluI A'CGCG_T
Cuts at: 6546 6546
Size: 6670
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2796 3227 3645 5784 5968 1305
Size: 1491 431 418 2139 184 2007
Fragments arranged by size:
2139 2007 1491 431 418 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2350 3243 10
Size: 54 1425 343 518 893 3437
Fragments arranged by size:
3437 1425 893 518 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2304 2344 4628 4987 5146 518
Size: 648 1138 40 2284 359 159 2042
Fragments arranged by size:
2284 2042 1138 648 359 159 40
MspA1I CmG'CkG
Cuts at: 1606 3514 3681 3699 4086 4763 5911 6156 1606
Size: 1908 167 18 387 677 1148 245 2120
Fragments arranged by size:
2120 1908 1148 677 387 245 167 18
NcoI C'CATG_G
Cuts at: 513 1065 2004 2345 2949 3244 513
Size: 552 939 341 604 295 3939
Fragments arranged by size:
3939 939 604 552 341 295
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6670
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6670
NotI GC'GGCC_GC
Cuts at: 4117 4117
Size: 6670
NspI r_CATG'y
Cuts at: 1132 1229 2416 3814 4028 5573 1132
Size: 97 1187 1398 214 1545 2229
Fragments arranged by size:
2229 1545 1398 1187 214 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6670
PciI A'CATG_T
Cuts at: 5569 5569
Size: 6670
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6670
PfoI T'CCnGG_A
Cuts at: 2814 3933 2814
Size: 1119 5551
PmeI GTTT'AAAC
Cuts at: 3974 3974
Size: 6670
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4739 4771 6252 6284 4739
Size: 32 1481 32 5125
Fragments arranged by size:
5125 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2010 2286 2010
Size: 276 6394
PshAI GACnn'nnGTC
Cuts at: 1613 2211 2331 1613
Size: 598 120 5952
Fragments arranged by size:
5952 598 120
PsiI TTA'TAA
Cuts at: 4240 4240
Size: 6670
PspOMI G'GGCC_C
Cuts at: 2992 3143 3412 2992
Size: 151 269 6250
Fragments arranged by size:
6250 269 151
PspXI vC'TCGA_Gb
Cuts at: 3124 3124
Size: 6670
PstI C_TGCA'G
Cuts at: 830 2048 2270 3396 4022 830
Size: 1218 222 1126 626 3478
Fragments arranged by size:
3478 1218 1126 626 222
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6670
PvuII CAG'CTG
Cuts at: 3514 3514
Size: 6670
SalI G'TCGA_C
Cuts at: 1936 4012 1936
Size: 2076 4594
SbfI CC_TGCA'GG
Cuts at: 4022 4022
Size: 6670
ScaI AGT'ACT
Cuts at: 4918 6630 4918
Size: 1712 4958
SexAI A'CCwGG_T
Cuts at: 2592 2592
Size: 6670
SfcI C'TryA_G
Cuts at: 826 1045 2044 2266 2329 3392 4018 5153 5834
Size: 219 999 222 63 1063 626 1135 681
Cuts at: 5834 6025 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1063 999 681 626 222 219
191 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6670
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6670
SmaI CCC'GGG
Cuts at: 1872 3997 1872
Size: 2125 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3124 3593 4774 5675 5937 820
Size: 197 926 1181 469 1181 901 262 1553
Fragments arranged by size:
1553 1181 1181 926 901 469 262 197
SnaBI TAC'GTA
Cuts at: 493 4461 493
Size: 3968 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6670
SphI G_CATG'C
Cuts at: 1132 4028 1132
Size: 2896 3774
SspI AAT'ATT
Cuts at: 5 52 4594 5
Size: 47 4542 2081
Fragments arranged by size:
4542 2081 47
StuI AGG'CCT
Cuts at: 1517 2144 1517
Size: 627 6043
StyI C'CwwG_G
Cuts at: 513 1065 2004 2345 2616 2949 3244 4109 513
Size: 552 939 341 271 333 295 865 3074
Fragments arranged by size:
3074 939 865 552 341 333 295 271
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2403 3524 4697 4714 4867 5052 5497
Size: 1255 823 1121 1173 17 153 185 445
Cuts at: 5497 325
Size: 1498
Fragments arranged by size:
1498 1255 1173 1121 823 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4916 6628 96
Size: 274 80 33 51 1443 2939 1712 138
Fragments arranged by size:
2939 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2489 2521 2728 2760 6630 6662 2489
Size: 32 207 32 3870 32 2497
Fragments arranged by size:
3870 2497 207 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2630 1316
Size: 1314 5356
XbaI T'CTAG_A
Cuts at: 4006 4006
Size: 6670
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3166 3166
Size: 6670
XhoI C'TCGA_G
Cuts at: 1943 3124 1943
Size: 1181 5489
XmaI C'CCGG_G
Cuts at: 1870 3995 1870
Size: 2125 4545
XmnI GAAnn'nnTTC
Cuts at: 3855 4799 3855
Size: 944 5726
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4478 276
Size: 53 83 186 936 2944 2468
Fragments arranged by size:
2944 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PsrI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI