Restriction map for
pFN21AE1474
(Circular) (Six-base) MAPSORT of: pFN21AE1474.seq Check: 491 from: 1 to: 6211
pFN21AE1474 6211 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3448 3448
Size: 6211
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3204 4021 278
Size: 53 83 186 936 1668 817 2468
Fragments arranged by size:
2468 1668 936 817 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3342 3554 1937
Size: 1405 212 4594
Fragments arranged by size:
4594 1405 212
Acc65I G'GTAC_C
Cuts at: 3221 3532 3221
Size: 311 5900
AclI AA'CG_TT
Cuts at: 1563 4338 4711 1563
Size: 2775 373 3063
Fragments arranged by size:
3063 2775 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2688 4274 5658 5926 1632
Size: 1056 1586 1384 268 1917
Fragments arranged by size:
1917 1586 1384 1056 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6014
AflIII A'CryG_T
Cuts at: 1203 5110 6087 1203
Size: 3907 977 1327
Fragments arranged by size:
3907 1327 977
AgeI A'CCGG_T
Cuts at: 5042 5042
Size: 6211
AhdI GACnn_n'nnGTC
Cuts at: 4940 4940
Size: 6211
AleI CACnn'nnGTG
Cuts at: 1166 2912 1166
Size: 1746 4465
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3165 1184
Size: 1981 4230
AlwNI CAG_nnn'CTG
Cuts at: 2723 2784 3390 3954 5526 2723
Size: 61 606 564 1572 3408
Fragments arranged by size:
3408 1572 606 564 61
ApaI G_GGCC'C
Cuts at: 2419 2419
Size: 6211
ApaLI G'TGCA_C
Cuts at: 4268 5424 4268
Size: 1156 5055
ApoI r'AATT_y
Cuts at: 1493 2470 3062 3520 3746 3898 6007 1493
Size: 977 592 458 226 152 2109 1697
Fragments arranged by size:
2109 1697 977 592 458 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4765 160
Size: 872 3733 1606
Fragments arranged by size:
3733 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6211
AvaI C'yCGr_G
Cuts at: 1870 1943 2055 2128 3536 1870
Size: 73 112 73 1408 4545
Fragments arranged by size:
4545 1408 112 73 73
BamHI G'GATC_C
Cuts at: 2545 3541 3908 2545
Size: 996 367 4848
Fragments arranged by size:
4848 996 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3221 3532 4987 618
Size: 325 217 627 1434 311 1455 1842
Fragments arranged by size:
1842 1455 1434 627 325 311 217
BanII G_rGCy'C
Cuts at: 1391 2419 3530 1391
Size: 1028 1111 4072
Fragments arranged by size:
4072 1111 1028
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2969 3175 928
Size: 971 1070 206 3964
Fragments arranged by size:
3964 1070 971 206
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2635 2669 3359 3393 4402 4436 1903
Size: 34 698 34 690 34 1009 34 3678
Fragments arranged by size:
3678 1009 698 690 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4103 5313 4103
Size: 1210 5001
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6211
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3495 4822 136
Size: 107 122 71 631 605 1823 1327 1525
Fragments arranged by size:
1823 1525 1327 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6206 6206
Size: 6211
BlpI GC'TnA_GC
Cuts at: 2021 3628 2021
Size: 1607 4604
Bme1580I G_kGCm'C
Cuts at: 1790 2419 4272 5428 1790
Size: 629 1853 1156 2573
Fragments arranged by size:
2573 1853 1156 629
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6211
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2097 2733 3243
Size: 416 53 83 399 24 680 636 510
Cuts at: 3243 3942 4900 442
Size: 699 958 1753
Fragments arranged by size:
1753 958 699 680 636 510 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2082 4871 1239
Size: 843 2789 2579
Fragments arranged by size:
2789 2579 843
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2158 3246 4336 5201 5499 2158
Size: 1088 1090 865 298 2870
Fragments arranged by size:
2870 1090 1088 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4874 882
Size: 617 3375 2219
Fragments arranged by size:
3375 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3334 4002 493
Size: 711 2130 668 2702
Fragments arranged by size:
2702 2130 711 668
BsaBI GATnn'nnATC
Cuts at: 6205 6205
Size: 6211
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2829 3201 4018 4400
Size: 53 83 186 936 1296 372 817 382
Cuts at: 4400 5035 275
Size: 635 1451
Fragments arranged by size:
1451 1296 936 817 635 382 372 186
83 53
BsaWI w'CCGG_w
Cuts at: 4644 5042 5316 5463 5874 4644
Size: 398 274 147 411 4981
Fragments arranged by size:
4981 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2590 2620 1178
Size: 30 165 30 1187 30 4769
Fragments arranged by size:
4769 1187 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2591 2873 1186
Size: 1405 282 4524
Fragments arranged by size:
4524 1405 282
BseYI C'CCAG_C
Cuts at: 2211 5414 5910 2211
Size: 3203 496 2512
Fragments arranged by size:
3203 2512 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2059 2768 3161 3308 3453 1163
Size: 573 323 709 393 147 145 3921
Fragments arranged by size:
3921 709 573 393 323 147 145
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2067 3661 4422 5450 1540
Size: 275 186 66 1594 761 1028 2301
Fragments arranged by size:
2301 1594 1028 761 275 186 66
BsiHKAI G_wGCw'C
Cuts at: 2814 4272 4357 5428 2814
Size: 1458 85 1071 3597
Fragments arranged by size:
3597 1458 1071 85
BsmI GAATG_Cn'
Cuts at: 3722 3815 3722
Size: 93 6118
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2419 2814 3530 4272 4357 5428 1391
Size: 399 629 395 716 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 716 629 399 395 85
BspEI T'CCGG_A
Cuts at: 5874 5874
Size: 6211
BspHI T'CATG_A
Cuts at: 3408 4100 5830 3408
Size: 692 1730 3789
Fragments arranged by size:
3789 1730 692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3448 3566 844
Size: 2604 118 3489
Fragments arranged by size:
3489 2604 118
BsrBI CCG'CTC
Cuts at: 1394 1823 4098 1394
Size: 429 2275 3507
Fragments arranged by size:
3507 2275 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3076 4706 4880 65
Size: 1183 1828 1630 174 1396
Fragments arranged by size:
1828 1630 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4855 5042 1700
Size: 110 3045 187 2869
Fragments arranged by size:
3045 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6211
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6211
BssSI C'ACGA_G
Cuts at: 2012 4271 5283 2012
Size: 2259 1012 2940
Fragments arranged by size:
2940 2259 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2784 794
Size: 1990 4221
BstBI TT'CG_AA
Cuts at: 5015 5015
Size: 6211
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6211
BstXI CCAn_nnnn'nTGG
Cuts at: 2611 3144 2611
Size: 533 5678
BstYI r'GATC_y
Cuts at: 1972 2545 2928 3541 3908 4293 4310 5762 5848
Size: 573 383 613 367 385 17 1452 86
Cuts at: 5848 5860 6206 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 613 573 385 383 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5659
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3231 487
Size: 683 56 2005 3467
Fragments arranged by size:
3467 2005 683 56
BtsI GCAGTG_nn'
Cuts at: 2556 3149 3304 3420 3727 4521 4541 2556
Size: 593 155 116 307 794 20 4226
Fragments arranged by size:
4226 794 593 307 155 116 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6211
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6176
DraI TTT'AAA
Cuts at: 3515 3515
Size: 6211
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3179 5218 809
Size: 507 1863 2039 1802
Fragments arranged by size:
2039 1863 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2904 3658
Size: 54 1425 50 275 18 198 876 754
Cuts at: 3658 4547 6176 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 876 754 275 198 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3658 1537
Size: 275 1846 4090
Fragments arranged by size:
4090 1846 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3275 4141 1353
Size: 1922 866 3423
Fragments arranged by size:
3423 1922 866
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4798 5172 5318 387
Size: 1064 138 3209 374 146 1280
Fragments arranged by size:
3209 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2082 2688 4274 4871 5658 5926 1239
Size: 393 450 606 1586 597 787 268 1524
Fragments arranged by size:
1586 1524 787 606 597 450 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3410 3410
Size: 6211
EcoO109I rG'GnC_Cy
Cuts at: 2143 2415 2143
Size: 272 5939
EcoRI G'AATT_C
Cuts at: 3520 3898 3520
Size: 378 5833
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2986 2986
Size: 6211
FspI TGC'GCA
Cuts at: 4717 4717
Size: 6211
HaeII r_GCGC'y
Cuts at: 1678 2049 5358 1678
Size: 371 3309 2531
Fragments arranged by size:
3309 2531 371
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4852 4884 4926 4958 1373
Size: 32 135 32 3280 32 42 32 2626
Fragments arranged by size:
3280 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3205 3555 3801 669
Size: 988 281 1267 350 246 3079
Fragments arranged by size:
3079 1267 988 350 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6211
HpaI GTT'AAC
Cuts at: 3801 3801
Size: 6211
KpnI G_GTAC'C
Cuts at: 3225 3536 3225
Size: 311 5900
MfeI C'AATT_G
Cuts at: 3810 3810
Size: 6211
MluI A'CGCG_T
Cuts at: 6087 6087
Size: 6211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2662 2815 5325 5509 1305
Size: 1357 153 2510 184 2007
Fragments arranged by size:
2510 2007 1357 184 153
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2906 10
Size: 54 1425 343 1074 3315
Fragments arranged by size:
3315 1425 1074 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2465 2912 4169 4528 4687 518
Size: 648 1299 447 1257 359 159 2042
Fragments arranged by size:
2042 1299 1257 648 447 359 159
MspA1I CmG'CkG
Cuts at: 1606 2298 2752 3627 4304 5452 5697 1606
Size: 692 454 875 677 1148 245 2120
Fragments arranged by size:
2120 1148 875 692 677 454 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5659
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6211
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6211
NotI GC'GGCC_GC
Cuts at: 3658 3658
Size: 6211
NspI r_CATG'y
Cuts at: 1132 1229 3569 5114 1132
Size: 97 2340 1545 2229
Fragments arranged by size:
2340 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2611 1348
Size: 1263 4948
PciI A'CATG_T
Cuts at: 5110 5110
Size: 6211
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2723 1108
Size: 1615 4596
PmeI GTTT'AAAC
Cuts at: 3515 3515
Size: 6211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4280 4312 5793 5825 4280
Size: 32 1481 32 4666
Fragments arranged by size:
4666 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2143 2143
Size: 6211
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6211
PsiI TTA'TAA
Cuts at: 3781 3781
Size: 6211
PspOMI G'GGCC_C
Cuts at: 2415 2415
Size: 6211
PstI C_TGCA'G
Cuts at: 830 3299 3563 830
Size: 2469 264 3478
Fragments arranged by size:
3478 2469 264
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6211
PvuII CAG'CTG
Cuts at: 2298 2752 2298
Size: 454 5757
SalI G'TCGA_C
Cuts at: 1936 3553 1936
Size: 1617 4594
SbfI CC_TGCA'GG
Cuts at: 3563 3563
Size: 6211
ScaI AGT'ACT
Cuts at: 4459 6171 4459
Size: 1712 4499
SfcI C'TryA_G
Cuts at: 826 1045 2293 2385 3295 3559 4694 5375 5566
Size: 219 1248 92 910 264 1135 681 191
Cuts at: 5566 826
Size: 1471
Fragments arranged by size:
1471 1248 1135 910 681 264 219 191
92
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6211
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6211
SmaI CCC'GGG
Cuts at: 1872 2130 3538 1872
Size: 258 1408 4545
Fragments arranged by size:
4545 1408 258
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2137 3261 4315 5216 5478 820
Size: 197 926 194 1124 1054 901 262 1553
Fragments arranged by size:
1553 1124 1054 926 901 262 197 194
SnaBI TAC'GTA
Cuts at: 493 4002 493
Size: 3509 2702
SpeI A'CTAG_T
Cuts at: 152 2168 152
Size: 2016 4195
SphI G_CATG'C
Cuts at: 1132 3569 1132
Size: 2437 3774
SspI AAT'ATT
Cuts at: 5 52 2425 4135 5
Size: 47 2373 1710 2081
Fragments arranged by size:
2373 2081 1710 47
StuI AGG'CCT
Cuts at: 1517 2324 1517
Size: 807 5404
StyI C'CwwG_G
Cuts at: 513 1065 3507 3650 513
Size: 552 2442 143 3074
Fragments arranged by size:
3074 2442 552 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2902 4238 4255 4408 4593 5038 325
Size: 1255 1322 1336 17 153 185 445 1498
Fragments arranged by size:
1498 1336 1322 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4457 6169 96
Size: 274 80 33 51 1443 2480 1712 138
Fragments arranged by size:
2480 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2590 2622 2793 2825 6171 6203 2590
Size: 32 171 32 3346 32 2598
Fragments arranged by size:
3346 2598 171 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3176 1316
Size: 1860 4351
XbaI T'CTAG_A
Cuts at: 3547 3547
Size: 6211
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2100 2914 2100
Size: 814 5397
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6211
XmaI C'CCGG_G
Cuts at: 1870 2128 3536 1870
Size: 258 1408 4545
Fragments arranged by size:
4545 1408 258
XmnI GAAnn'nnTTC
Cuts at: 3066 4340 3066
Size: 1274 4937
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3202 4019 276
Size: 53 83 186 936 1668 817 2468
Fragments arranged by size:
2468 1668 936 817 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI