Restriction map for
pFN21AE1471
(Circular) (Six-base) MAPSORT of: pFN21AE1471.seq Check: 8497 from: 1 to: 5413
pFN21AE1471 5413 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3223 278
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2756 1937
Size: 819 4594
Acc65I G'GTAC_C
Cuts at: 2734 2734
Size: 5413
AclI AA'CG_TT
Cuts at: 1563 3540 3913 1563
Size: 1977 373 3063
Fragments arranged by size:
3063 1977 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3476 4860 5128 1632
Size: 1844 1384 268 1917
Fragments arranged by size:
1917 1844 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5216
AflIII A'CryG_T
Cuts at: 1203 2277 4312 5289 1203
Size: 1074 2035 977 1327
Fragments arranged by size:
2035 1327 1074 977
AgeI A'CCGG_T
Cuts at: 4244 4244
Size: 5413
AhdI GACnn_n'nnGTC
Cuts at: 4142 4142
Size: 5413
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5413
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5413
AlwNI CAG_nnn'CTG
Cuts at: 2512 3156 4728 2512
Size: 644 1572 3197
Fragments arranged by size:
3197 1572 644
ApaI G_GGCC'C
Cuts at: 2148 2148
Size: 5413
ApaLI G'TGCA_C
Cuts at: 3470 4626 3470
Size: 1156 4257
ApoI r'AATT_y
Cuts at: 1493 2722 2948 3100 5209 1493
Size: 1229 226 152 2109 1697
Fragments arranged by size:
2109 1697 1229 226 152
AscI GG'CGCG_CC
Cuts at: 2183 2183
Size: 5413
AseI AT'TA_AT
Cuts at: 160 1032 3967 160
Size: 872 2935 1606
Fragments arranged by size:
2935 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
AvaI C'yCGr_G
Cuts at: 1870 1943 2738 1870
Size: 73 795 4545
Fragments arranged by size:
4545 795 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2266 2299 2266
Size: 33 5380
BamHI G'GATC_C
Cuts at: 2743 3110 2743
Size: 367 5046
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2250 2551 2590 2596 2734
Size: 325 217 627 463 301 39 6 138
Cuts at: 2734 4189 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 463 325 301 217 138
39 6
BanII G_rGCy'C
Cuts at: 1391 2055 2148 2406 2612 2732 1391
Size: 664 93 258 206 120 4072
Fragments arranged by size:
4072 664 258 206 120 93
BbeI G_GCGC'C
Cuts at: 2594 2600 2594
Size: 6 5407
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2588 928
Size: 971 689 3753
Fragments arranged by size:
3753 971 689
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3604 3638 1903
Size: 34 1667 34 3678
Fragments arranged by size:
3678 1667 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3305 4515 3305
Size: 1210 4203
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5413
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4024 136
Size: 107 122 71 631 605 2352 1525
Fragments arranged by size:
2352 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5408 5408
Size: 5413
BlpI GC'TnA_GC
Cuts at: 2830 2830
Size: 5413
Bme1580I G_kGCm'C
Cuts at: 1790 2025 2148 2556 3474 4630 1790
Size: 235 123 408 918 1156 2573
Fragments arranged by size:
2573 1156 918 408 235 123
BmgBI CAC'GTC
Cuts at: 1318 2278 1318
Size: 960 4453
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2134 3144 4102
Size: 416 53 83 399 24 717 1010 958
Cuts at: 4102 442
Size: 1753
Fragments arranged by size:
1753 1010 958 717 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5413
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2350 2710 2350
Size: 360 5053
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 4073 1239
Size: 830 2004 2579
Fragments arranged by size:
2579 2004 830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2039 2174 3538 4403 4701 2039
Size: 135 1364 865 298 2751
Fragments arranged by size:
2751 1364 865 298 135
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4076 882
Size: 617 2577 2219
Fragments arranged by size:
2577 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3204 493
Size: 711 2000 2702
Fragments arranged by size:
2702 2000 711
BsaBI GATnn'nnATC
Cuts at: 5407 5407
Size: 5413
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2433 2591 2597 3220
Size: 53 83 186 936 900 158 6 623
Cuts at: 3220 3602 4237 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 900 635 623 382 186 158
83 53 6
BsaWI w'CCGG_w
Cuts at: 2499 3846 4244 4518 4665 5076 2499
Size: 1347 398 274 147 411 2836
Fragments arranged by size:
2836 1347 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5188
Fragments arranged by size:
5188 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2669 2672 2720 1186
Size: 1483 3 48 3879
Fragments arranged by size:
3879 1483 48 3
BseYI C'CCAG_C
Cuts at: 2024 4616 5112 2024
Size: 2592 496 2325
Fragments arranged by size:
2592 2325 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4840
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2015 2079 2863 3624 4652 1540
Size: 275 186 14 64 784 761 1028 2301
Fragments arranged by size:
2301 1028 784 761 275 186 64 14
BsiHKAI G_wGCw'C
Cuts at: 2055 3474 3559 4630 2055
Size: 1419 85 1071 2838
Fragments arranged by size:
2838 1419 1071 85
BsmI GAATG_Cn'
Cuts at: 2924 3017 2924
Size: 93 5320
BsmBI CGTCTCn'nnnn_
Cuts at: 2261 2261
Size: 5413
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2055 2148 2406 2556 2612 2732
Size: 399 235 30 93 258 150 56 120
Cuts at: 2732 3474 3559 4630 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 258 235 150 120
93 85 56 30
BspEI T'CCGG_A
Cuts at: 2499 5076 2499
Size: 2577 2836
BspHI T'CATG_A
Cuts at: 3302 5032 3302
Size: 1730 3683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2497 2768 844
Size: 1653 271 3489
Fragments arranged by size:
3489 1653 271
BsrBI CCG'CTC
Cuts at: 1394 1823 2322 3300 1394
Size: 429 499 978 3507
Fragments arranged by size:
3507 978 499 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3908 4082 65
Size: 1183 2660 174 1396
Fragments arranged by size:
2660 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2074 2302 2593 4057 4244 1700
Size: 110 264 228 291 1464 187 2869
Fragments arranged by size:
2869 1464 291 264 228 187 110
BsrGI T'GTAC_A
Cuts at: 96 2445 96
Size: 2349 3064
BssHII G'CGCG_C
Cuts at: 1925 2183 1925
Size: 258 5155
BssSI C'ACGA_G
Cuts at: 3473 4485 3473
Size: 1012 4401
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5413
BstBI TT'CG_AA
Cuts at: 4217 4217
Size: 5413
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5413
BstXI CCAn_nnnn'nTGG
Cuts at: 2563 2563
Size: 5413
BstYI r'GATC_y
Cuts at: 1972 2743 3110 3495 3512 4964 5050 5062 5408
Size: 771 367 385 17 1452 86 12 346
Cuts at: 5408 1972
Size: 1977
Fragments arranged by size:
1977 1452 771 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3922
Fragments arranged by size:
3922 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4674
Fragments arranged by size:
4674 683 56
BtsI GCAGTG_nn'
Cuts at: 2929 3723 3743 2929
Size: 794 20 4599
Fragments arranged by size:
4599 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5413
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5378
DraI TTT'AAA
Cuts at: 2717 2717
Size: 5413
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4420 809
Size: 507 3104 1802
Fragments arranged by size:
3104 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2012 2133 2565
Size: 54 1425 50 275 18 182 121 432
Cuts at: 2565 2860 3749 5378 8
Size: 295 889 1629 43
Fragments arranged by size:
1629 1425 889 432 295 275 182 121
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2012 2860 1537
Size: 275 200 848 4090
Fragments arranged by size:
4090 848 275 200
EarI CTCTTCn'nnn_
Cuts at: 1353 2064 2624 3343 1353
Size: 711 560 719 3423
Fragments arranged by size:
3423 719 711 560
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4000 4374 4520 387
Size: 1064 138 2411 374 146 1280
Fragments arranged by size:
2411 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2053 2053
Size: 5413
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 3476 4073 4860 5128 1239
Size: 393 437 1407 597 787 268 1524
Fragments arranged by size:
1524 1407 787 597 437 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2338 2338
Size: 5413
EcoO109I rG'GnC_Cy
Cuts at: 2145 2509 2145
Size: 364 5049
EcoRI G'AATT_C
Cuts at: 2722 3100 2722
Size: 378 5035
FspI TGC'GCA
Cuts at: 3919 3919
Size: 5413
HaeII r_GCGC'y
Cuts at: 1678 2042 2594 2600 4560 1678
Size: 364 552 6 1960 2531
Fragments arranged by size:
2531 1960 552 364 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2594 2626 4054 4086 4128
Size: 32 135 32 1022 32 1428 32 42
Cuts at: 4128 4160 1373
Size: 32 2626
Fragments arranged by size:
2626 1428 1022 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2757 3003 669
Size: 988 281 819 246 3079
Fragments arranged by size:
3079 988 819 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5413
HpaI GTT'AAC
Cuts at: 3003 3003
Size: 5413
KasI G'GCGC_C
Cuts at: 2590 2596 2590
Size: 6 5407
KpnI G_GTAC'C
Cuts at: 2738 2738
Size: 5413
MfeI C'AATT_G
Cuts at: 3012 3012
Size: 5413
MluI A'CGCG_T
Cuts at: 5289 5289
Size: 5413
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4527 4711 1305
Size: 3222 184 2007
Fragments arranged by size:
3222 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2135 10
Size: 54 1425 343 303 3288
Fragments arranged by size:
3288 1425 343 303 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3371 3730 3889 518
Size: 648 2205 359 159 2042
Fragments arranged by size:
2205 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2670 2829 3506 4654 4899 1606
Size: 1064 159 677 1148 245 2120
Fragments arranged by size:
2120 1148 1064 677 245 159
NaeI GCC'GGC
Cuts at: 2595 2595
Size: 5413
NarI GG'CG_CC
Cuts at: 2591 2597 2591
Size: 6 5407
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3922
Fragments arranged by size:
3922 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5413
NgoMIV G'CCGG_C
Cuts at: 2593 2593
Size: 5413
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5413
NotI GC'GGCC_GC
Cuts at: 2860 2860
Size: 5413
NspI r_CATG'y
Cuts at: 1132 1229 2771 4316 1132
Size: 97 1542 1545 2229
Fragments arranged by size:
2229 1545 1542 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5413
PciI A'CATG_T
Cuts at: 4312 4312
Size: 5413
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2247 1108
Size: 1139 4274
PmeI GTTT'AAAC
Cuts at: 2717 2717
Size: 5413
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3482 3514 4995 5027 3482
Size: 32 1481 32 3868
Fragments arranged by size:
3868 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2509 2509
Size: 5413
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5413
PsiI TTA'TAA
Cuts at: 2983 2983
Size: 5413
PspOMI G'GGCC_C
Cuts at: 2144 2144
Size: 5413
PstI C_TGCA'G
Cuts at: 830 2067 2765 830
Size: 1237 698 3478
Fragments arranged by size:
3478 1237 698
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5413
PvuII CAG'CTG
Cuts at: 2670 2670
Size: 5413
SacI G_AGCT'C
Cuts at: 2055 2055
Size: 5413
SalI G'TCGA_C
Cuts at: 1936 2755 1936
Size: 819 4594
SapI GCTCTTCn'nnn_
Cuts at: 2064 2064
Size: 5413
SbfI CC_TGCA'GG
Cuts at: 2765 2765
Size: 5413
ScaI AGT'ACT
Cuts at: 2573 3661 5373 2573
Size: 1088 1712 2613
Fragments arranged by size:
2613 1712 1088
SfcI C'TryA_G
Cuts at: 826 1045 2063 2761 3896 4577 4768 826
Size: 219 1018 698 1135 681 191 1471
Fragments arranged by size:
1471 1135 1018 698 681 219 191
SfoI GGC'GCC
Cuts at: 2592 2598 2592
Size: 6 5407
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
SgrAI Cr'CCGG_yG
Cuts at: 1700 2302 2593 1700
Size: 602 291 4520
Fragments arranged by size:
4520 602 291
SmaI CCC'GGG
Cuts at: 1872 2740 1872
Size: 868 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2054 2189 3517 4418 4680 820
Size: 197 926 111 135 1328 901 262 1553
Fragments arranged by size:
1553 1328 926 901 262 197 135 111
SnaBI TAC'GTA
Cuts at: 493 3204 493
Size: 2711 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5413
SphI G_CATG'C
Cuts at: 1132 2771 1132
Size: 1639 3774
SspI AAT'ATT
Cuts at: 5 52 3337 5
Size: 47 3285 2081
Fragments arranged by size:
3285 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5413
StyI C'CwwG_G
Cuts at: 513 1065 2004 2520 2586 2852 513
Size: 552 939 516 66 266 3074
Fragments arranged by size:
3074 939 552 516 266 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3440 3457 3610 3795 4240 325
Size: 1255 1860 17 153 185 445 1498
Fragments arranged by size:
1860 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2445 2484 2571
Size: 274 80 33 51 1443 468 39 87
Cuts at: 2571 3659 5371 96
Size: 1088 1712 138
Fragments arranged by size:
1712 1443 1088 468 274 138 87 80
51 39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2076 2108 5373 5405 2076
Size: 32 3265 32 2084
Fragments arranged by size:
3265 2084 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5413
XbaI T'CTAG_A
Cuts at: 2749 2749
Size: 5413
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5413
XmaI C'CCGG_G
Cuts at: 1870 2738 1870
Size: 868 4545
XmnI GAAnn'nnTTC
Cuts at: 3542 3542
Size: 5413
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3221 276
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BbvCI BfrBI Bpu10I BsiWI
BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SrfI SwaI XcmI