Restriction map for
pFN21AE1469
(Circular) (Six-base) MAPSORT of: pFN21AE1469.seq Check: 6034 from: 1 to: 4984
pFN21AE1469 4984 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2794 278
Size: 53 83 186 936 1258 2468
Fragments arranged by size:
2468 1258 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2327 1937
Size: 390 4594
Acc65I G'GTAC_C
Cuts at: 2305 2305
Size: 4984
AclI AA'CG_TT
Cuts at: 1563 3111 3484 1563
Size: 1548 373 3063
Fragments arranged by size:
3063 1548 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2197 3047 4431 4699 1632
Size: 565 850 1384 268 1917
Fragments arranged by size:
1917 1384 850 565 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4787
AflIII A'CryG_T
Cuts at: 1203 3883 4860 1203
Size: 2680 977 1327
Fragments arranged by size:
2680 1327 977
AgeI A'CCGG_T
Cuts at: 3815 3815
Size: 4984
AhdI GACnn_n'nnGTC
Cuts at: 3713 3713
Size: 4984
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4984
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4984
AlwNI CAG_nnn'CTG
Cuts at: 2109 2727 4299 2109
Size: 618 1572 2794
Fragments arranged by size:
2794 1572 618
ApaLI G'TGCA_C
Cuts at: 3041 4197 3041
Size: 1156 3828
ApoI r'AATT_y
Cuts at: 1493 2293 2519 2671 4780 1493
Size: 800 226 152 2109 1697
Fragments arranged by size:
2109 1697 800 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3538 160
Size: 872 2506 1606
Fragments arranged by size:
2506 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4984
AvaI C'yCGr_G
Cuts at: 1870 1943 2185 2309 1870
Size: 73 242 124 4545
Fragments arranged by size:
4545 242 124 73
BamHI G'GATC_C
Cuts at: 2314 2681 2314
Size: 367 4617
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2087 2305 3760 618
Size: 325 217 627 300 218 1455 1842
Fragments arranged by size:
1842 1455 627 325 300 218 217
BanII G_rGCy'C
Cuts at: 1391 2303 1391
Size: 912 4072
BbeI G_GCGC'C
Cuts at: 2091 2091
Size: 4984
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2106 928
Size: 971 207 3806
Fragments arranged by size:
3806 971 207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3175 3209 1903
Size: 34 1238 34 3678
Fragments arranged by size:
3678 1238 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2876 4086 2876
Size: 1210 3774
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4984
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2085 3595 136
Size: 107 122 71 631 605 413 1510 1525
Fragments arranged by size:
1525 1510 631 605 413 122 107 71
BglII A'GATC_T
Cuts at: 2226 4979 2226
Size: 2753 2231
BlpI GC'TnA_GC
Cuts at: 2276 2401 2276
Size: 125 4859
Bme1580I G_kGCm'C
Cuts at: 1790 2250 3045 4201 1790
Size: 460 795 1156 2573
Fragments arranged by size:
2573 1156 795 460
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4984
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2715 3673 442
Size: 416 53 83 399 24 1298 958 1753
Fragments arranged by size:
1753 1298 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4984
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3644 1239
Size: 2405 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3109 3974 4272 3109
Size: 865 298 3821
Fragments arranged by size:
3821 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3647 882
Size: 617 2148 2219
Fragments arranged by size:
2219 2148 617
BsaAI yAC'GTr
Cuts at: 493 1204 2775 493
Size: 711 1571 2702
Fragments arranged by size:
2702 1571 711
BsaBI GATnn'nnATC
Cuts at: 4978 4978
Size: 4984
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2088 2791 3173 3808
Size: 53 83 186 936 555 703 382 635
Cuts at: 3808 275
Size: 1451
Fragments arranged by size:
1451 936 703 635 555 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3417 3815 4089 4236 4647 3417
Size: 398 274 147 411 3754
Fragments arranged by size:
3754 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4759
Fragments arranged by size:
4759 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4984
BseYI C'CCAG_C
Cuts at: 4187 4683 4187
Size: 496 4488
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2007 1163
Size: 573 271 4140
Fragments arranged by size:
4140 573 271
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2434 3195 4223 1540
Size: 275 186 433 761 1028 2301
Fragments arranged by size:
2301 1028 761 433 275 186
BsiHKAI G_wGCw'C
Cuts at: 3045 3130 4201 3045
Size: 85 1071 3828
Fragments arranged by size:
3828 1071 85
BsmI GAATG_Cn'
Cuts at: 2495 2588 2495
Size: 93 4891
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2250 2303 3045 3130 4201 1391
Size: 399 460 53 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 460 399 85 53
BspEI T'CCGG_A
Cuts at: 4647 4647
Size: 4984
BspHI T'CATG_A
Cuts at: 2873 4603 2873
Size: 1730 3254
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2339 844
Size: 1495 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2871 1394
Size: 429 1048 3507
Fragments arranged by size:
3507 1048 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3479 3653 65
Size: 1183 2231 174 1396
Fragments arranged by size:
2231 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3628 3815 1700
Size: 110 1818 187 2869
Fragments arranged by size:
2869 1818 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4984
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4984
BssSI C'ACGA_G
Cuts at: 3044 4056 3044
Size: 1012 3972
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4984
BstBI TT'CG_AA
Cuts at: 3788 3788
Size: 4984
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4984
BstYI r'GATC_y
Cuts at: 1972 2226 2314 2681 3066 3083 4535 4621 4633
Size: 254 88 367 385 17 1452 86 12
Cuts at: 4633 4979 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 254 88 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3493
Fragments arranged by size:
3493 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4245
Fragments arranged by size:
4245 683 56
BtsI GCAGTG_nn'
Cuts at: 2500 3294 3314 2500
Size: 794 20 4170
Fragments arranged by size:
4170 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4984
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4949
DraI TTT'AAA
Cuts at: 2288 2288
Size: 4984
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3991 809
Size: 507 2675 1802
Fragments arranged by size:
2675 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2431 3320 4949
Size: 54 1425 50 275 18 601 889 1629
Cuts at: 4949 8
Size: 43
Fragments arranged by size:
1629 1425 889 601 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2431 1537
Size: 275 619 4090
Fragments arranged by size:
4090 619 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2914 1353
Size: 1561 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3571 3945 4091 387
Size: 1064 138 1982 374 146 1280
Fragments arranged by size:
1982 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2197 3047 3644 4431 4699 1239
Size: 393 565 850 597 787 268 1524
Fragments arranged by size:
1524 850 787 597 565 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2185 2185
Size: 4984
EcoO109I rG'GnC_Cy
Cuts at: 2078 2078
Size: 4984
EcoRI G'AATT_C
Cuts at: 2293 2671 2293
Size: 378 4606
FspI TGC'GCA
Cuts at: 3490 3490
Size: 4984
HaeII r_GCGC'y
Cuts at: 1678 2060 2091 4131 1678
Size: 382 31 2040 2531
Fragments arranged by size:
2531 2040 382 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3625 3657 3699 3731 1373
Size: 32 135 32 2053 32 42 32 2626
Fragments arranged by size:
2626 2053 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2328 2574 669
Size: 988 281 390 246 3079
Fragments arranged by size:
3079 988 390 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4984
HpaI GTT'AAC
Cuts at: 2574 2574
Size: 4984
KasI G'GCGC_C
Cuts at: 2087 2087
Size: 4984
KpnI G_GTAC'C
Cuts at: 2309 2309
Size: 4984
MfeI C'AATT_G
Cuts at: 2583 2583
Size: 4984
MluI A'CGCG_T
Cuts at: 4860 4860
Size: 4984
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4098 4282 1305
Size: 2793 184 2007
Fragments arranged by size:
2793 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3162
Fragments arranged by size:
3162 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2942 3301 3460 518
Size: 648 1776 359 159 2042
Fragments arranged by size:
2042 1776 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2400 3077 4225 4470 1606
Size: 794 677 1148 245 2120
Fragments arranged by size:
2120 1148 794 677 245
NarI GG'CG_CC
Cuts at: 2088 2088
Size: 4984
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3493
Fragments arranged by size:
3493 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4984
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4984
NotI GC'GGCC_GC
Cuts at: 2431 2431
Size: 4984
NspI r_CATG'y
Cuts at: 1132 1229 2342 3887 1132
Size: 97 1113 1545 2229
Fragments arranged by size:
2229 1545 1113 97
PasI CC'CwG_GG
Cuts at: 1348 2250 1348
Size: 902 4082
PciI A'CATG_T
Cuts at: 3883 3883
Size: 4984
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4984
PmeI GTTT'AAAC
Cuts at: 2288 2288
Size: 4984
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3053 3085 4566 4598 3053
Size: 32 1481 32 3439
Fragments arranged by size:
3439 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4984
PsiI TTA'TAA
Cuts at: 2554 2554
Size: 4984
PstI C_TGCA'G
Cuts at: 830 2336 830
Size: 1506 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4984
SalI G'TCGA_C
Cuts at: 1936 2326 1936
Size: 390 4594
SbfI CC_TGCA'GG
Cuts at: 2336 2336
Size: 4984
ScaI AGT'ACT
Cuts at: 3232 4944 3232
Size: 1712 3272
SfcI C'TryA_G
Cuts at: 826 1045 2015 2332 3467 4148 4339 826
Size: 219 970 317 1135 681 191 1471
Fragments arranged by size:
1471 1135 970 681 317 219 191
SfoI GGC'GCC
Cuts at: 2089 2089
Size: 4984
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4984
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4984
SmaI CCC'GGG
Cuts at: 1872 2311 1872
Size: 439 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3088 3989 4251 820
Size: 197 926 1145 901 262 1553
Fragments arranged by size:
1553 1145 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2775 493
Size: 2282 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4984
SphI G_CATG'C
Cuts at: 1132 2342 1132
Size: 1210 3774
SspI AAT'ATT
Cuts at: 5 52 2908 5
Size: 47 2856 2081
Fragments arranged by size:
2856 2081 47
StuI AGG'CCT
Cuts at: 1517 2191 1517
Size: 674 4310
StyI C'CwwG_G
Cuts at: 513 1065 2004 2423 513
Size: 552 939 419 3074
Fragments arranged by size:
3074 939 552 419
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2242 3011 3028 3181 3366 3811 325
Size: 1255 662 769 17 153 185 445 1498
Fragments arranged by size:
1498 1255 769 662 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3230 4942 96
Size: 274 80 33 51 1443 1253 1712 138
Fragments arranged by size:
1712 1443 1253 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4944 4976 4944
Size: 32 4952
Tth111I GACn'n_nGTC
Cuts at: 1316 2105 1316
Size: 789 4195
XbaI T'CTAG_A
Cuts at: 2320 2320
Size: 4984
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4984
XmaI C'CCGG_G
Cuts at: 1870 2309 1870
Size: 439 4545
XmnI GAAnn'nnTTC
Cuts at: 2159 3113 2159
Size: 954 4030
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2792 276
Size: 53 83 186 936 1258 2468
Fragments arranged by size:
2468 1258 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI XcmI