Restriction map for
pFN21AE1465
(Circular) (Six-base) MAPSORT of: pFN21AE1465.seq Check: 7395 from: 1 to: 5833
pFN21AE1465 5833 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3031 3031
Size: 5833
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3021 3643 278
Size: 53 83 186 936 1485 622 2468
Fragments arranged by size:
2468 1485 936 622 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2772 3176 1937
Size: 835 404 4594
Fragments arranged by size:
4594 835 404
Acc65I G'GTAC_C
Cuts at: 3154 3154
Size: 5833
AclI AA'CG_TT
Cuts at: 1563 3960 4333 1563
Size: 2397 373 3063
Fragments arranged by size:
3063 2397 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3896 5280 5548 1632
Size: 2264 1384 268 1917
Fragments arranged by size:
2264 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5636
AflIII A'CryG_T
Cuts at: 1203 4732 5709 1203
Size: 3529 977 1327
Fragments arranged by size:
3529 1327 977
AgeI A'CCGG_T
Cuts at: 4664 4664
Size: 5833
AhdI GACnn_n'nnGTC
Cuts at: 4562 4562
Size: 5833
AleI CACnn'nnGTG
Cuts at: 1166 3011 1166
Size: 1845 3988
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3098 1184
Size: 1914 3919
AlwNI CAG_nnn'CTG
Cuts at: 3003 3046 3576 5148 3003
Size: 43 530 1572 3688
Fragments arranged by size:
3688 1572 530 43
ApaLI G'TGCA_C
Cuts at: 3890 5046 3890
Size: 1156 4677
ApoI r'AATT_y
Cuts at: 1493 3142 3368 3520 5629 1493
Size: 1649 226 152 2109 1697
Fragments arranged by size:
2109 1697 1649 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4387 160
Size: 872 3355 1606
Fragments arranged by size:
3355 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
AvaI C'yCGr_G
Cuts at: 1870 1943 3158 1870
Size: 73 1215 4545
Fragments arranged by size:
4545 1215 73
BamHI G'GATC_C
Cuts at: 3163 3530 3163
Size: 367 5466
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2469 2716 2803 3154 4609
Size: 325 217 627 682 247 87 351 1455
Cuts at: 4609 618
Size: 1842
Fragments arranged by size:
1842 1455 682 627 351 325 247 217
87
BanII G_rGCy'C
Cuts at: 1391 2040 2547 3152 1391
Size: 649 507 605 4072
Fragments arranged by size:
4072 649 605 507
BbeI G_GCGC'C
Cuts at: 2807 2807
Size: 5833
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2446 928
Size: 971 547 4315
Fragments arranged by size:
4315 971 547
BbvCI CC'TCA_GC
Cuts at: 2465 2734 2465
Size: 269 5564
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4024 4058 1903
Size: 34 50 34 2003 34 3678
Fragments arranged by size:
3678 2003 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3725 4935 3725
Size: 1210 4623
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5833
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2725 4444 136
Size: 107 122 71 631 605 1053 1719 1525
Fragments arranged by size:
1719 1525 1053 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2565 5828 2565
Size: 3263 2570
BlpI GC'TnA_GC
Cuts at: 3250 3250
Size: 5833
Bme1580I G_kGCm'C
Cuts at: 1790 3894 5050 1790
Size: 2104 1156 2573
Fragments arranged by size:
2573 2104 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5833
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2053 2927 3564
Size: 416 53 83 399 24 636 874 637
Cuts at: 3564 4522 442
Size: 958 1753
Fragments arranged by size:
1753 958 874 637 636 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2199 2495 2612 2621 2969 4493 1239
Size: 960 296 117 9 348 1524 2579
Fragments arranged by size:
2579 1524 960 348 296 117 9
Bpu10I CC'TnA_GC
Cuts at: 2465 2734 2465
Size: 269 5564
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2279 2762 2912 3958 4823 5121 2279
Size: 483 150 1046 865 298 2991
Fragments arranged by size:
2991 1046 865 483 298 150
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2141 4496 882
Size: 617 642 2355 2219
Fragments arranged by size:
2355 2219 642 617
BsaAI yAC'GTr
Cuts at: 493 1204 3624 493
Size: 711 2420 2702
Fragments arranged by size:
2702 2420 711
BsaBI GATnn'nnATC
Cuts at: 5827 5827
Size: 5833
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2373 2804 3018 3640
Size: 53 83 186 936 840 431 214 622
Cuts at: 3640 4022 4657 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 840 635 622 431 382 214
186 83 53
BsaWI w'CCGG_w
Cuts at: 2670 4266 4664 4938 5085 5496 2670
Size: 1596 398 274 147 411 3007
Fragments arranged by size:
3007 1596 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2468 2498 1178
Size: 30 165 30 1065 30 4513
Fragments arranged by size:
4513 1065 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5833
BseYI C'CCAG_C
Cuts at: 5036 5532 5036
Size: 496 5337
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2989 1163
Size: 573 1253 4007
Fragments arranged by size:
4007 1253 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3283 4044 5072 1540
Size: 275 186 1282 761 1028 2301
Fragments arranged by size:
2301 1282 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2547 2763 3894 3979 5050 2547
Size: 216 1131 85 1071 3330
Fragments arranged by size:
3330 1131 1071 216 85
BsmI GAATG_Cn'
Cuts at: 3344 3437 3344
Size: 93 5740
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2547 2763 3152 3894 3979 5050
Size: 399 250 507 216 389 742 85 1071
Cuts at: 5050 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 507 399 389 250 216
85
BspEI T'CCGG_A
Cuts at: 5496 5496
Size: 5833
BspHI T'CATG_A
Cuts at: 3722 5452 3722
Size: 1730 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2310 3031 3188 844
Size: 1466 721 157 3489
Fragments arranged by size:
3489 1466 721 157
BsrBI CCG'CTC
Cuts at: 1394 1823 3720 1394
Size: 429 1897 3507
Fragments arranged by size:
3507 1897 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4328 4502 65
Size: 1183 3080 174 1396
Fragments arranged by size:
3080 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4477 4664 1700
Size: 110 2667 187 2869
Fragments arranged by size:
2869 2667 187 110
BsrGI T'GTAC_A
Cuts at: 96 2751 96
Size: 2655 3178
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5833
BssSI C'ACGA_G
Cuts at: 3893 4905 3893
Size: 1012 4821
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3003 3046 794
Size: 2209 43 3581
Fragments arranged by size:
3581 2209 43
BstBI TT'CG_AA
Cuts at: 4637 4637
Size: 5833
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5833
BstYI r'GATC_y
Cuts at: 1972 2218 2565 2637 2925 3163 3530 3915 3932
Size: 246 347 72 288 238 367 385 17
Cuts at: 3932 5384 5470 5482 5828 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 347 346 288 246
238 86 72 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2265 2413 3009 513
Size: 552 1200 148 596 3337
Fragments arranged by size:
3337 1200 596 552 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5094
Fragments arranged by size:
5094 683 56
BtsI GCAGTG_nn'
Cuts at: 2015 3349 4143 4163 2015
Size: 1334 794 20 3685
Fragments arranged by size:
3685 1334 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5798
DraI TTT'AAA
Cuts at: 3137 3137
Size: 5833
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4840 809
Size: 507 3524 1802
Fragments arranged by size:
3524 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2268 3280 4169
Size: 54 1425 50 275 18 438 1012 889
Cuts at: 4169 5798 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1012 889 438 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3280 1537
Size: 275 1468 4090
Fragments arranged by size:
4090 1468 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2045 3763 1353
Size: 692 1718 3423
Fragments arranged by size:
3423 1718 692
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4420 4794 4940 387
Size: 1064 138 2831 374 146 1280
Fragments arranged by size:
2831 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2545 2545
Size: 5833
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2199 2495 2612 2621 2969 3896 4493
Size: 393 567 296 117 9 348 927 597
Cuts at: 4493 5280 5548 1239
Size: 787 268 1524
Fragments arranged by size:
1524 927 787 597 567 393 348 296
268 117 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2051 2711 2051
Size: 660 5173
EcoO109I rG'GnC_Cy
Cuts at: 2575 2665 2575
Size: 90 5743
EcoRI G'AATT_C
Cuts at: 3142 3520 3142
Size: 378 5455
FspI TGC'GCA
Cuts at: 4339 4339
Size: 5833
HaeII r_GCGC'y
Cuts at: 1678 2807 3081 4980 1678
Size: 1129 274 1899 2531
Fragments arranged by size:
2531 1899 1129 274
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2503 2535 4474 4506 4548
Size: 32 135 32 931 32 1939 32 42
Cuts at: 4548 4580 1373
Size: 32 2626
Fragments arranged by size:
2626 1939 931 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2938 3177 3423 669
Size: 988 281 1000 239 246 3079
Fragments arranged by size:
3079 1000 988 281 246 239
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5833
HpaI GTT'AAC
Cuts at: 3423 3423
Size: 5833
KasI G'GCGC_C
Cuts at: 2803 2803
Size: 5833
KpnI G_GTAC'C
Cuts at: 3158 3158
Size: 5833
MfeI C'AATT_G
Cuts at: 3432 3432
Size: 5833
MluI A'CGCG_T
Cuts at: 5709 5709
Size: 5833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2650 2889 4947 5131 1305
Size: 1345 239 2058 184 2007
Fragments arranged by size:
2058 2007 1345 239 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4011
Fragments arranged by size:
4011 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2270 2766 3011 3791 4150 4309 518
Size: 648 1104 496 245 780 359 159 2042
Fragments arranged by size:
2042 1104 780 648 496 359 245 159
MspA1I CmG'CkG
Cuts at: 1606 2749 2917 3249 3926 5074 5319 1606
Size: 1143 168 332 677 1148 245 2120
Fragments arranged by size:
2120 1148 1143 677 332 245 168
NarI GG'CG_CC
Cuts at: 2804 2804
Size: 5833
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5281
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5833
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5833
NotI GC'GGCC_GC
Cuts at: 3280 3280
Size: 5833
NspI r_CATG'y
Cuts at: 1132 1229 3191 4736 1132
Size: 97 1962 1545 2229
Fragments arranged by size:
2229 1962 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5833
PciI A'CATG_T
Cuts at: 4732 4732
Size: 5833
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2647 1108
Size: 902 637 4294
Fragments arranged by size:
4294 902 637
PfoI T'CCnGG_A
Cuts at: 2364 2640 2364
Size: 276 5557
PmeI GTTT'AAAC
Cuts at: 3137 3137
Size: 5833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3902 3934 5415 5447 3902
Size: 32 1481 32 4288
Fragments arranged by size:
4288 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2575 2575
Size: 5833
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5833
PsiI TTA'TAA
Cuts at: 2700 3403 2700
Size: 703 5130
PstI C_TGCA'G
Cuts at: 830 2922 3042 3185 830
Size: 2092 120 143 3478
Fragments arranged by size:
3478 2092 143 120
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5833
PvuII CAG'CTG
Cuts at: 2749 2917 2749
Size: 168 5665
SacI G_AGCT'C
Cuts at: 2547 2547
Size: 5833
SalI G'TCGA_C
Cuts at: 1936 3175 1936
Size: 1239 4594
SanDI GG'GwC_CC
Cuts at: 2575 2575
Size: 5833
SapI GCTCTTCn'nnn_
Cuts at: 2045 2045
Size: 5833
SbfI CC_TGCA'GG
Cuts at: 3185 3185
Size: 5833
ScaI AGT'ACT
Cuts at: 4081 5793 4081
Size: 1712 4121
SfcI C'TryA_G
Cuts at: 826 1045 2918 3038 3181 4316 4997 5188 826
Size: 219 1873 120 143 1135 681 191 1471
Fragments arranged by size:
1873 1471 1135 681 219 191 143 120
SfoI GGC'GCC
Cuts at: 2805 2805
Size: 5833
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5833
SmaI CCC'GGG
Cuts at: 1872 3160 1872
Size: 1288 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2294 2741 2891 3937 4838 5100
Size: 197 926 351 447 150 1046 901 262
Cuts at: 5100 820
Size: 1553
Fragments arranged by size:
1553 1046 926 901 447 351 262 197
150
SnaBI TAC'GTA
Cuts at: 493 3624 493
Size: 3131 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5833
SphI G_CATG'C
Cuts at: 1132 3191 1132
Size: 2059 3774
SspI AAT'ATT
Cuts at: 5 52 3757 5
Size: 47 3705 2081
Fragments arranged by size:
3705 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5833
StyI C'CwwG_G
Cuts at: 513 1065 2965 3272 513
Size: 552 1900 307 3074
Fragments arranged by size:
3074 1900 552 307
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2269 3860 3877 4030 4215 4660 325
Size: 1255 689 1591 17 153 185 445 1498
Fragments arranged by size:
1591 1498 1255 689 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2274 2334 2751
Size: 274 80 33 51 1443 297 60 417
Cuts at: 2751 4079 5791 96
Size: 1328 1712 138
Fragments arranged by size:
1712 1443 1328 417 297 274 138 80
60 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2244 2276 5793 5825 2244
Size: 32 3517 32 2252
Fragments arranged by size:
3517 2252 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5833
XbaI T'CTAG_A
Cuts at: 3169 3169
Size: 5833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2437 2587 2854 2437
Size: 150 267 5416
Fragments arranged by size:
5416 267 150
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5833
XmaI C'CCGG_G
Cuts at: 1870 3158 1870
Size: 1288 4545
XmnI GAAnn'nnTTC
Cuts at: 2453 3962 2453
Size: 1509 4324
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3019 3641 276
Size: 53 83 186 936 1485 622 2468
Fragments arranged by size:
2468 1485 936 622 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SexAI SfiI SrfI
SwaI