Restriction map for
pFN21AE1463
(Circular) (Six-base) MAPSORT of: pFN21AE1463.seq Check: 9669 from: 1 to: 5155
pFN21AE1463 5155 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2068 2068
Size: 5155
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2965 278
Size: 53 83 186 936 1429 2468
Fragments arranged by size:
2468 1429 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2151 2259 2498 1937
Size: 214 108 239 4594
Fragments arranged by size:
4594 239 214 108
Acc65I G'GTAC_C
Cuts at: 2476 2476
Size: 5155
AclI AA'CG_TT
Cuts at: 1563 3282 3655 1563
Size: 1719 373 3063
Fragments arranged by size:
3063 1719 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3218 4602 4870 1632
Size: 1586 1384 268 1917
Fragments arranged by size:
1917 1586 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4958
AflIII A'CryG_T
Cuts at: 1203 4054 5031 1203
Size: 2851 977 1327
Fragments arranged by size:
2851 1327 977
AgeI A'CCGG_T
Cuts at: 3986 3986
Size: 5155
AhdI GACnn_n'nnGTC
Cuts at: 3884 3884
Size: 5155
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5155
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5155
AlwNI CAG_nnn'CTG
Cuts at: 2898 4470 2898
Size: 1572 3583
ApaLI G'TGCA_C
Cuts at: 3212 4368 3212
Size: 1156 3999
ApoI r'AATT_y
Cuts at: 1493 2464 2690 2842 4951 1493
Size: 971 226 152 2109 1697
Fragments arranged by size:
2109 1697 971 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3709 160
Size: 872 2677 1606
Fragments arranged by size:
2677 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5155
AvaI C'yCGr_G
Cuts at: 1870 1943 2135 2480 1870
Size: 73 192 345 4545
Fragments arranged by size:
4545 345 192 73
BamHI G'GATC_C
Cuts at: 2485 2852 2485
Size: 367 4788
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2320 2476 3931 618
Size: 325 217 627 533 156 1455 1842
Fragments arranged by size:
1842 1455 627 533 325 217 156
BanII G_rGCy'C
Cuts at: 1391 2161 2474 1391
Size: 770 313 4072
Fragments arranged by size:
4072 770 313
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4184
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3346 3380 1903
Size: 34 1409 34 3678
Fragments arranged by size:
3678 1409 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3047 4257 3047
Size: 1210 3945
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5155
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3766 136
Size: 107 122 71 631 605 2094 1525
Fragments arranged by size:
2094 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5150 5150
Size: 5155
BlpI GC'TnA_GC
Cuts at: 2572 2572
Size: 5155
Bme1580I G_kGCm'C
Cuts at: 1790 3216 4372 1790
Size: 1426 1156 2573
Fragments arranged by size:
2573 1426 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5155
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2886 3844 442
Size: 416 53 83 399 24 1469 958 1753
Fragments arranged by size:
1753 1469 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3815 1239
Size: 2576 2579
Bpu10I CC'TnA_GC
Cuts at: 2312 2312
Size: 5155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3280 4145 4443 3280
Size: 865 298 3992
Fragments arranged by size:
3992 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3818 882
Size: 617 2319 2219
Fragments arranged by size:
2319 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2946 493
Size: 711 1742 2702
Fragments arranged by size:
2702 1742 711
BsaBI GATnn'nnATC
Cuts at: 5149 5149
Size: 5155
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2962 3344 3979 275
Size: 53 83 186 936 1429 382 635 1451
Fragments arranged by size:
1451 1429 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2433 3588 3986 4260 4407 4818 2433
Size: 1155 398 274 147 411 2770
Fragments arranged by size:
2770 1155 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2285 2315 1178
Size: 30 165 30 882 30 4018
Fragments arranged by size:
4018 882 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2411 1186
Size: 1225 3930
BseYI C'CCAG_C
Cuts at: 4358 4854 4358
Size: 496 4659
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4582
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2403 2605 3366 4394 1540
Size: 275 186 402 202 761 1028 2301
Fragments arranged by size:
2301 1028 761 402 275 202 186
BsiHKAI G_wGCw'C
Cuts at: 3216 3301 4372 3216
Size: 85 1071 3999
Fragments arranged by size:
3999 1071 85
BsmI GAATG_Cn'
Cuts at: 2666 2759 2666
Size: 93 5062
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2161 2474 3216 3301 4372 1391
Size: 399 371 313 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 371 313 85
BspEI T'CCGG_A
Cuts at: 2433 4818 2433
Size: 2385 2770
BspHI T'CATG_A
Cuts at: 2227 3044 4774 2227
Size: 817 1730 2608
Fragments arranged by size:
2608 1730 817
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2068 2510 844
Size: 1224 442 3489
Fragments arranged by size:
3489 1224 442
BsrBI CCG'CTC
Cuts at: 1394 1823 2119 3042 1394
Size: 429 296 923 3507
Fragments arranged by size:
3507 923 429 296
BsrDI GCAATG_nn'
Cuts at: 65 1248 3650 3824 65
Size: 1183 2402 174 1396
Fragments arranged by size:
2402 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2126 2213 3799 3986 1700
Size: 110 316 87 1586 187 2869
Fragments arranged by size:
2869 1586 316 187 110 87
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5155
BssHII G'CGCG_C
Cuts at: 1925 2090 2270 2297 2337 2339 2341 2410 1925
Size: 165 180 27 40 2 2 69 4670
Fragments arranged by size:
4670 180 165 69 40 27 2 2
BssSI C'ACGA_G
Cuts at: 3215 4227 3215
Size: 1012 4143
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5155
BstBI TT'CG_AA
Cuts at: 3959 3959
Size: 5155
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5155
BstYI r'GATC_y
Cuts at: 1972 2485 2852 3237 3254 4706 4792 4804 5150
Size: 513 367 385 17 1452 86 12 346
Cuts at: 5150 1972
Size: 1977
Fragments arranged by size:
1977 1452 513 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2447 513
Size: 552 1382 3221
Fragments arranged by size:
3221 1382 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4416
Fragments arranged by size:
4416 683 56
BtsI GCAGTG_nn'
Cuts at: 2671 3465 3485 2671
Size: 794 20 4341
Fragments arranged by size:
4341 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5155
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5120
DraI TTT'AAA
Cuts at: 2459 2459
Size: 5155
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4162 809
Size: 507 2846 1802
Fragments arranged by size:
2846 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2400 2602 3491
Size: 54 1425 50 275 18 570 202 889
Cuts at: 3491 5120 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 570 275 202 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2400 2602 1537
Size: 275 588 202 4090
Fragments arranged by size:
4090 588 275 202
EarI CTCTTCn'nnn_
Cuts at: 1353 3085 1353
Size: 1732 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3742 4116 4262 387
Size: 1064 138 2153 374 146 1280
Fragments arranged by size:
2153 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3218 3815 4602 4870 1239
Size: 393 1586 597 787 268 1524
Fragments arranged by size:
1586 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2097 2097
Size: 5155
EcoRI G'AATT_C
Cuts at: 2464 2842 2464
Size: 378 4777
FspI TGC'GCA
Cuts at: 3661 3661
Size: 5155
HaeII r_GCGC'y
Cuts at: 1678 2366 4302 1678
Size: 688 1936 2531
Fragments arranged by size:
2531 1936 688
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3796 3828 3870 3902 1373
Size: 32 135 32 2224 32 42 32 2626
Fragments arranged by size:
2626 2224 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2499 2745 669
Size: 988 281 561 246 3079
Fragments arranged by size:
3079 988 561 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5155
HpaI GTT'AAC
Cuts at: 2745 2745
Size: 5155
KpnI G_GTAC'C
Cuts at: 2480 2480
Size: 5155
MfeI C'AATT_G
Cuts at: 2754 2754
Size: 5155
MluI A'CGCG_T
Cuts at: 5031 5031
Size: 5155
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4269 4453 1305
Size: 2964 184 2007
Fragments arranged by size:
2964 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3333
Fragments arranged by size:
3333 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3113 3472 3631 518
Size: 648 1947 359 159 2042
Fragments arranged by size:
2042 1947 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2188 2326 2571 3248 4396 4641 1606
Size: 582 138 245 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 582 245 245 138
NaeI GCC'GGC
Cuts at: 2215 2215
Size: 5155
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4603
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5155
NgoMIV G'CCGG_C
Cuts at: 2213 2213
Size: 5155
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5155
NotI GC'GGCC_GC
Cuts at: 2602 2602
Size: 5155
NspI r_CATG'y
Cuts at: 1132 1229 2513 4058 1132
Size: 97 1284 1545 2229
Fragments arranged by size:
2229 1545 1284 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5155
PciI A'CATG_T
Cuts at: 4054 4054
Size: 5155
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2205 1108
Size: 1097 4058
PmeI GTTT'AAAC
Cuts at: 2459 2459
Size: 5155
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3224 3256 4737 4769 3224
Size: 32 1481 32 3610
Fragments arranged by size:
3610 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5155
PsiI TTA'TAA
Cuts at: 2725 2725
Size: 5155
PstI C_TGCA'G
Cuts at: 830 2079 2174 2507 830
Size: 1249 95 333 3478
Fragments arranged by size:
3478 1249 333 95
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5155
SalI G'TCGA_C
Cuts at: 1936 2497 1936
Size: 561 4594
SbfI CC_TGCA'GG
Cuts at: 2079 2507 2079
Size: 428 4727
ScaI AGT'ACT
Cuts at: 2180 3403 5115 2180
Size: 1223 1712 2220
Fragments arranged by size:
2220 1712 1223
SfcI C'TryA_G
Cuts at: 826 1045 2075 2170 2503 3638 4319 4510 826
Size: 219 1030 95 333 1135 681 191 1471
Fragments arranged by size:
1471 1135 1030 681 333 219 191 95
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5155
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5155
SmaI CCC'GGG
Cuts at: 1872 2482 1872
Size: 610 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3259 4160 4422 820
Size: 197 926 1316 901 262 1553
Fragments arranged by size:
1553 1316 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2946 493
Size: 2453 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5155
SphI G_CATG'C
Cuts at: 1132 2513 1132
Size: 1381 3774
SspI AAT'ATT
Cuts at: 5 52 3079 5
Size: 47 3027 2081
Fragments arranged by size:
3027 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5155
StyI C'CwwG_G
Cuts at: 513 1065 2594 513
Size: 552 1529 3074
Fragments arranged by size:
3074 1529 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3182 3199 3352 3537 3982 325
Size: 1255 1602 17 153 185 445 1498
Fragments arranged by size:
1602 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2178 3401 5113
Size: 274 80 33 51 1443 201 1223 1712
Cuts at: 5113 96
Size: 138
Fragments arranged by size:
1712 1443 1223 274 201 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5115 5147 5115
Size: 32 5123
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5155
XbaI T'CTAG_A
Cuts at: 2491 2491
Size: 5155
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5155
XmaI C'CCGG_G
Cuts at: 1870 2480 1870
Size: 610 4545
XmnI GAAnn'nnTTC
Cuts at: 3284 3284
Size: 5155
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2963 276
Size: 53 83 186 936 1429 2468
Fragments arranged by size:
2468 1429 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI