Restriction map for
pFN21AE1459
(Circular) (Six-base) MAPSORT of: pFN21AE1459.seq Check: 3068 from: 1 to: 7930
pFN21AE1459 7930 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4024 4024
Size: 7930
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5740 278
Size: 53 83 186 936 4204 2468
Fragments arranged by size:
4204 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5273 1937
Size: 3336 4594
Acc65I G'GTAC_C
Cuts at: 5251 5251
Size: 7930
AclI AA'CG_TT
Cuts at: 1563 4084 6057 6430 1563
Size: 2521 1973 373 3063
Fragments arranged by size:
3063 2521 1973 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2862 3057 3372 4128 4829 5993 7377 7645
Size: 1230 195 315 756 701 1164 1384 268
Cuts at: 7645 1632
Size: 1917
Fragments arranged by size:
1917 1384 1230 1164 756 701 315 268
195
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7733
AflIII A'CryG_T
Cuts at: 1203 5133 6829 7806 1203
Size: 3930 1696 977 1327
Fragments arranged by size:
3930 1696 1327 977
AgeI A'CCGG_T
Cuts at: 6761 6761
Size: 7930
AhdI GACnn_n'nnGTC
Cuts at: 6659 6659
Size: 7930
AleI CACnn'nnGTG
Cuts at: 1166 2835 1166
Size: 1669 6261
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2853 1184
Size: 1669 6261
AlwNI CAG_nnn'CTG
Cuts at: 4700 4769 4880 5673 7245 4700
Size: 69 111 793 1572 5385
Fragments arranged by size:
5385 1572 793 111 69
ApaLI G'TGCA_C
Cuts at: 4037 5987 7143 4037
Size: 1950 1156 4824
Fragments arranged by size:
4824 1950 1156
ApoI r'AATT_y
Cuts at: 1493 2420 3570 4481 4576 5239 5465 5617 7726
Size: 927 1150 911 95 663 226 152 2109
Cuts at: 7726 1493
Size: 1697
Fragments arranged by size:
2109 1697 1150 927 911 663 226 152
95
AseI AT'TA_AT
Cuts at: 160 1032 4461 6484 160
Size: 872 3429 2023 1606
Fragments arranged by size:
3429 2023 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7930
AvaI C'yCGr_G
Cuts at: 1870 1943 2089 3108 5188 5255 1870
Size: 73 146 1019 2080 67 4545
Fragments arranged by size:
4545 2080 1019 146 73 67
BamHI G'GATC_C
Cuts at: 2621 5260 5627 2621
Size: 2639 367 4924
Fragments arranged by size:
4924 2639 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2024 2071 2077 5251 6706
Size: 325 217 627 237 47 6 3174 1455
Cuts at: 6706 618
Size: 1842
Fragments arranged by size:
3174 1842 1455 627 325 237 217 47
6
BanII G_rGCy'C
Cuts at: 1391 2040 2096 2774 3340 3979 5229 5249 1391
Size: 649 56 678 566 639 1250 20 4072
Fragments arranged by size:
4072 1250 678 649 639 566 56 20
BbeI G_GCGC'C
Cuts at: 2028 2075 2081 2028
Size: 47 6 7877
Fragments arranged by size:
7877 47 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2475 3521 4134 4395 4445 928
Size: 971 576 1046 613 261 50 4413
Fragments arranged by size:
4413 1046 971 613 576 261 50
BbvCI CC'TCA_GC
Cuts at: 4839 4839
Size: 7930
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6121 6155 1903
Size: 34 4184 34 3678
Fragments arranged by size:
4184 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3156 5822 7032 3156
Size: 2666 1210 4054
Fragments arranged by size:
4054 2666 1210
BclI T'GATC_A
Cuts at: 1548 3172 1548
Size: 1624 6306
BfrBI ATG'CAT
Cuts at: 2540 3485 4271 4912 2540
Size: 945 786 641 5558
Fragments arranged by size:
5558 945 786 641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6541 136
Size: 107 122 71 631 605 4869 1525
Fragments arranged by size:
4869 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2107 4679 5216 7925 2107
Size: 2572 537 2709 2112
Fragments arranged by size:
2709 2572 2112 537
BlpI GC'TnA_GC
Cuts at: 2768 5347 2768
Size: 2579 5351
Bme1580I G_kGCm'C
Cuts at: 1790 2330 4041 5991 7147 1790
Size: 540 1711 1950 1156 2573
Fragments arranged by size:
2573 1950 1711 1156 540
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7930
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3393 3519 3723
Size: 416 53 83 399 24 1976 126 204
Cuts at: 3723 5661 6619 442
Size: 1938 958 1753
Fragments arranged by size:
1976 1938 1753 958 416 399 204 126
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7930
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2465 2465
Size: 7930
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2634 2685 2818 3226 4677 4881 6590 1239
Size: 1395 51 133 408 1451 204 1709 2579
Fragments arranged by size:
2579 1709 1451 1395 408 204 133 51
Bpu10I CC'TnA_GC
Cuts at: 4839 4873 4839
Size: 34 7896
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2132 2483 3777 4917 6055 6920 7218 2132
Size: 351 1294 1140 1138 865 298 2844
Fragments arranged by size:
2844 1294 1140 1138 865 351 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6593 882
Size: 617 5094 2219
Fragments arranged by size:
5094 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2931 5721 493
Size: 711 1727 2790 2702
Fragments arranged by size:
2790 2702 1727 711
BsaBI GATnn'nnATC
Cuts at: 7924 7924
Size: 7930
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2025 2072 2078 5737
Size: 53 83 186 936 492 47 6 3659
Cuts at: 5737 6119 6754 275
Size: 382 635 1451
Fragments arranged by size:
3659 1451 936 635 492 382 186 83
53 47 6
BsaWI w'CCGG_w
Cuts at: 6363 6761 7035 7182 7593 6363
Size: 398 274 147 411 6700
Fragments arranged by size:
6700 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5151 5181 1178
Size: 30 165 30 3748 30 3927
Fragments arranged by size:
3927 3748 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4827 1186
Size: 3641 4289
BseYI C'CCAG_C
Cuts at: 2855 3780 4012 4786 7133 7629 2855
Size: 925 232 774 2347 496 3156
Fragments arranged by size:
3156 2347 925 774 496 232
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3636 4052 4950 1163
Size: 573 1900 416 898 4143
Fragments arranged by size:
4143 1900 898 573 416
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5380 6141 7169 1540
Size: 275 186 3379 761 1028 2301
Fragments arranged by size:
3379 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2040 3779 4041 5991 6076 7147 2040
Size: 1739 262 1950 85 1071 2823
Fragments arranged by size:
2823 1950 1739 1071 262 85
BsmI GAATG_Cn'
Cuts at: 4912 4914 5441 5534 4912
Size: 2 527 93 7308
Fragments arranged by size:
7308 527 93 2
BsmBI CGTCTCn'nnnn_
Cuts at: 2214 4731 4899 2214
Size: 2517 168 5245
Fragments arranged by size:
5245 2517 168
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2096 2330 2774 3340 3779 3979
Size: 399 250 56 234 444 566 439 200
Cuts at: 3979 4041 5229 5249 5991 6076 7147 1391
Size: 62 1188 20 742 85 1071 2174
Fragments arranged by size:
2174 1188 1071 742 566 444 439 399
250 234 200 85 62 56 20
BspEI T'CCGG_A
Cuts at: 7593 7593
Size: 7930
BspHI T'CATG_A
Cuts at: 3850 5819 7549 3850
Size: 1969 1730 4231
Fragments arranged by size:
4231 1969 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3608 4024 5285 844
Size: 2764 416 1261 3489
Fragments arranged by size:
3489 2764 1261 416
BsrBI CCG'CTC
Cuts at: 1394 1823 5817 1394
Size: 429 3994 3507
Fragments arranged by size:
3994 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4060 6425 6599 65
Size: 1183 2812 2365 174 1396
Fragments arranged by size:
2812 2365 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2074 6574 6761 1700
Size: 110 264 4500 187 2869
Fragments arranged by size:
4500 2869 264 187 110
BsrGI T'GTAC_A
Cuts at: 96 3712 4360 4434 96
Size: 3616 648 74 3592
Fragments arranged by size:
3616 3592 648 74
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7930
BssSI C'ACGA_G
Cuts at: 5990 7002 5990
Size: 1012 6918
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4630 794
Size: 3836 4094
BstBI TT'CG_AA
Cuts at: 3655 6734 3655
Size: 3079 4851
BstEII G'GTnAC_C
Cuts at: 1184 4279 1184
Size: 3095 4835
BstXI CCAn_nnnn'nTGG
Cuts at: 3267 3267
Size: 7930
BstYI r'GATC_y
Cuts at: 1972 2107 2152 2621 2741 3137 3869 4679 5216
Size: 135 45 469 120 396 732 810 537
Cuts at: 5216 5260 5627 6012 6029 7481 7567 7579 7925
Size: 44 367 385 17 1452 86 12 346
Cuts at: 7925 1972
Size: 1977
Fragments arranged by size:
1977 1452 810 732 537 469 396 385
367 346 135 120 86 45 44 17
12
Bsu36I CC'TnA_GG
Cuts at: 2785 2785
Size: 7930
BtgI C'CryG_G
Cuts at: 513 1065 2950 4275 513
Size: 552 1885 1325 4168
Fragments arranged by size:
4168 1885 1325 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7191
Fragments arranged by size:
7191 683 56
BtsI GCAGTG_nn'
Cuts at: 4035 4313 5446 6240 6260 4035
Size: 278 1133 794 20 5705
Fragments arranged by size:
5705 1133 794 278 20
ClaI AT'CG_AT
Cuts at: 1554 3466 1554
Size: 1912 6018
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7895
DraI TTT'AAA
Cuts at: 3153 5234 3153
Size: 2081 5849
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4449 6937 809
Size: 507 3133 2488 1802
Fragments arranged by size:
3133 2488 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3223 5377 6266
Size: 54 1425 50 275 18 1393 2154 889
Cuts at: 6266 7895 8
Size: 1629 43
Fragments arranged by size:
2154 1629 1425 1393 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5377 1537
Size: 275 3565 4090
Fragments arranged by size:
4090 3565 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2221 2521 4122 5860 1353
Size: 868 300 1601 1738 3423
Fragments arranged by size:
3423 1738 1601 868 300
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6517 6891 7037 387
Size: 1064 138 4928 374 146 1280
Fragments arranged by size:
4928 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2038 2038
Size: 7930
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2634 2685 2818 2862 3057 3226 3372
Size: 393 1002 51 133 44 195 169 146
Cuts at: 3372 4128 4677 4829 4881 5993 6590 7377 7645
Size: 756 549 152 52 1112 597 787 268
Cuts at: 7645 1239
Size: 1524
Fragments arranged by size:
1524 1112 1002 787 756 597 549 393
268 195 169 152 146 133 52 51
44
EcoNI CCTnn'n_nnAGG
Cuts at: 2797 4872 2797
Size: 2075 5855
EcoO109I rG'GnC_Cy
Cuts at: 2789 2859 2789
Size: 70 7860
EcoRI G'AATT_C
Cuts at: 5239 5617 5239
Size: 378 7552
EcoRV GAT'ATC
Cuts at: 4655 4655
Size: 7930
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3048 3048
Size: 7930
FspI TGC'GCA
Cuts at: 6436 6436
Size: 7930
HaeII r_GCGC'y
Cuts at: 1678 2028 2075 2081 7077 1678
Size: 350 47 6 4996 2531
Fragments arranged by size:
4996 2531 350 47 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2114 2146 2567 2599 2733
Size: 32 135 32 542 32 421 32 134
Cuts at: 2733 2765 4336 4368 4699 4731 4991 5023 6571
Size: 32 1571 32 331 32 260 32 1548
Cuts at: 6571 6603 6645 6677 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1571 1548 542 421 331 260 135
134 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4544 5274 5520 669
Size: 988 281 2606 730 246 3079
Fragments arranged by size:
3079 2606 988 730 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7930
HpaI GTT'AAC
Cuts at: 5520 5520
Size: 7930
KasI G'GCGC_C
Cuts at: 2024 2071 2077 2024
Size: 47 6 7877
Fragments arranged by size:
7877 47 6
KpnI G_GTAC'C
Cuts at: 5255 5255
Size: 7930
MfeI C'AATT_G
Cuts at: 4356 5529 4356
Size: 1173 6757
MluI A'CGCG_T
Cuts at: 7806 7806
Size: 7930
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7044 7228 1305
Size: 5739 184 2007
Fragments arranged by size:
5739 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3225 10
Size: 54 1425 343 1393 4715
Fragments arranged by size:
4715 1425 1393 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2388 2568 2835 4218 4907 5888 6247
Size: 648 1222 180 267 1383 689 981 359
Cuts at: 6247 6406 518
Size: 159 2042
Fragments arranged by size:
2042 1383 1222 981 689 648 359 267
180 159
MspA1I CmG'CkG
Cuts at: 1606 3784 4025 5346 6023 7171 7416 1606
Size: 2178 241 1321 677 1148 245 2120
Fragments arranged by size:
2178 2120 1321 1148 677 245 241
NaeI GCC'GGC
Cuts at: 2076 2076
Size: 7930
NarI GG'CG_CC
Cuts at: 2025 2072 2078 2025
Size: 47 6 7877
Fragments arranged by size:
7877 47 6
NcoI C'CATG_G
Cuts at: 513 1065 2950 4275 513
Size: 552 1885 1325 4168
Fragments arranged by size:
4168 1885 1325 552
NdeI CA'TA_TG
Cuts at: 387 4267 4341 4747 387
Size: 3880 74 406 3570
Fragments arranged by size:
3880 3570 406 74
NgoMIV G'CCGG_C
Cuts at: 2074 2074
Size: 7930
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7930
NotI GC'GGCC_GC
Cuts at: 5377 5377
Size: 7930
NsiI A_TGCA'T
Cuts at: 2542 3487 4273 4914 2542
Size: 945 786 641 5558
Fragments arranged by size:
5558 945 786 641
NspI r_CATG'y
Cuts at: 1132 1229 5137 5288 6833 1132
Size: 97 3908 151 1545 2229
Fragments arranged by size:
3908 2229 1545 151 97
PasI CC'CwG_GG
Cuts at: 1348 2907 1348
Size: 1559 6371
PciI A'CATG_T
Cuts at: 5133 6829 5133
Size: 1696 6234
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2198 2306 3221 5021 1108
Size: 1090 108 915 1800 4017
Fragments arranged by size:
4017 1800 1090 915 108
PmeI GTTT'AAAC
Cuts at: 5234 5234
Size: 7930
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5999 6031 7512 7544 5999
Size: 32 1481 32 6385
Fragments arranged by size:
6385 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2789 2859 2789
Size: 70 7860
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7930
PsiI TTA'TAA
Cuts at: 5500 5500
Size: 7930
PstI C_TGCA'G
Cuts at: 830 2107 2605 2977 3753 4030 5091 5282 830
Size: 1277 498 372 776 277 1061 191 3478
Fragments arranged by size:
3478 1277 1061 776 498 372 277 191
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7930
PvuII CAG'CTG
Cuts at: 4025 4025
Size: 7930
SacI G_AGCT'C
Cuts at: 2040 2040
Size: 7930
SalI G'TCGA_C
Cuts at: 1936 5272 1936
Size: 3336 4594
SanDI GG'GwC_CC
Cuts at: 2859 2859
Size: 7930
SbfI CC_TGCA'GG
Cuts at: 5282 5282
Size: 7930
ScaI AGT'ACT
Cuts at: 2993 6178 7890 2993
Size: 3185 1712 3033
Fragments arranged by size:
3185 3033 1712
SexAI A'CCwGG_T
Cuts at: 2893 3492 2893
Size: 599 7331
SfcI C'TryA_G
Cuts at: 826 1045 2103 2396 2601 2973 3749 4026 5087
Size: 219 1058 293 205 372 776 277 1061
Cuts at: 5087 5278 6413 7094 7285 826
Size: 191 1135 681 191 1471
Fragments arranged by size:
1471 1135 1061 1058 776 681 372 293
277 219 205 191 191
SfoI GGC'GCC
Cuts at: 2026 2073 2079 2026
Size: 47 6 7877
Fragments arranged by size:
7877 47 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7930
SgrAI Cr'CCGG_yG
Cuts at: 1700 2074 1700
Size: 374 7556
SmaI CCC'GGG
Cuts at: 1872 2091 3110 5257 1872
Size: 219 1019 2147 4545
Fragments arranged by size:
4545 2147 1019 219
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2111 2462 3792 4932 6034 6935
Size: 197 926 168 351 1330 1140 1102 901
Cuts at: 6935 7197 820
Size: 262 1553
Fragments arranged by size:
1553 1330 1140 1102 926 901 351 262
197 168
SnaBI TAC'GTA
Cuts at: 493 5721 493
Size: 5228 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7930
SphI G_CATG'C
Cuts at: 1132 5288 1132
Size: 4156 3774
SspI AAT'ATT
Cuts at: 5 52 5854 5
Size: 47 5802 2081
Fragments arranged by size:
5802 2081 47
StuI AGG'CCT
Cuts at: 1517 2434 2964 4189 1517
Size: 917 530 1225 5258
Fragments arranged by size:
5258 1225 917 530
StyI C'CwwG_G
Cuts at: 513 1065 2617 2950 2965 3088 3133 4275 4803
Size: 552 1552 333 15 123 45 1142 528
Cuts at: 4803 5155 5369 513
Size: 352 214 3074
Fragments arranged by size:
3074 1552 1142 552 528 352 333 214
123 45 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2728 5957 5974 6127 6312 6757 325
Size: 1255 1148 3229 17 153 185 445 1498
Fragments arranged by size:
3229 1498 1255 1148 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2991 3554 3712
Size: 274 80 33 51 1443 1014 563 158
Cuts at: 3712 3816 4222 4360 4434 6176 7888 96
Size: 104 406 138 74 1742 1712 138
Fragments arranged by size:
1742 1712 1443 1014 563 406 274 158
138 138 104 80 74 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5151 5183 7890 7922 5151
Size: 32 2707 32 5159
Fragments arranged by size:
5159 2707 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7930
XbaI T'CTAG_A
Cuts at: 2438 5266 2438
Size: 2828 5102
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7930
XmaI C'CCGG_G
Cuts at: 1870 2089 3108 5255 1870
Size: 219 1019 2147 4545
Fragments arranged by size:
4545 2147 1019 219
XmnI GAAnn'nnTTC
Cuts at: 4912 6059 4912
Size: 1147 6783
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5738 276
Size: 53 83 186 936 4204 2468
Fragments arranged by size:
4204 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BsiWI BstZ17I
DraIII FseI FspAI NruI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacII SapI SfiI SrfI SwaI
XcmI