Restriction map for
pFN21AE1454
(Circular) (Six-base) MAPSORT of: pFN21AE1454.seq Check: 8268 from: 1 to: 6655
pFN21AE1454 6655 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2568 2568
Size: 6655
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3466 4465 278
Size: 53 83 186 936 1930 999 2468
Fragments arranged by size:
2468 1930 999 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3998 1937
Size: 2061 4594
Acc65I G'GTAC_C
Cuts at: 3976 3976
Size: 6655
AclI AA'CG_TT
Cuts at: 1563 4782 5155 1563
Size: 3219 373 3063
Fragments arranged by size:
3219 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2786 3843 4718 6102 6370 1632
Size: 1154 1057 875 1384 268 1917
Fragments arranged by size:
1917 1384 1154 1057 875 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6458
AflIII A'CryG_T
Cuts at: 1203 5554 6531 1203
Size: 4351 977 1327
Fragments arranged by size:
4351 1327 977
AgeI A'CCGG_T
Cuts at: 5486 5486
Size: 6655
AhdI GACnn_n'nnGTC
Cuts at: 5384 5384
Size: 6655
AleI CACnn'nnGTG
Cuts at: 1166 3237 1166
Size: 2071 4584
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6655
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2781 2813 2781
Size: 32 6623
AlwNI CAG_nnn'CTG
Cuts at: 2114 3524 4398 5970 2114
Size: 1410 874 1572 2799
Fragments arranged by size:
2799 1572 1410 874
ApaI G_GGCC'C
Cuts at: 2054 2561 2861 3005 3281 3912 2054
Size: 507 300 144 276 631 4797
Fragments arranged by size:
4797 631 507 300 276 144
ApaLI G'TGCA_C
Cuts at: 2901 4712 5868 2901
Size: 1811 1156 3688
Fragments arranged by size:
3688 1811 1156
ApoI r'AATT_y
Cuts at: 1493 3146 3755 3964 4190 4342 6451 1493
Size: 1653 609 209 226 152 2109 1697
Fragments arranged by size:
2109 1697 1653 609 226 209 152
AseI AT'TA_AT
Cuts at: 160 1032 5209 160
Size: 872 4177 1606
Fragments arranged by size:
4177 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6655
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 2569 3980 1870
Size: 73 93 533 1411 4545
Fragments arranged by size:
4545 1411 533 93 73
AvrII C'CTAG_G
Cuts at: 2662 3713 2662
Size: 1051 5604
BamHI G'GATC_C
Cuts at: 2220 3352 3985 4352 2220
Size: 1132 633 367 4523
Fragments arranged by size:
4523 1132 633 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2076 3375 3976 5431 618
Size: 325 217 627 289 1299 601 1455 1842
Fragments arranged by size:
1842 1455 1299 627 601 325 289 217
BanII G_rGCy'C
Cuts at: 1391 2054 2561 2570 2655 2835 2861 3005 3212
Size: 663 507 9 85 180 26 144 207
Cuts at: 3212 3255 3281 3540 3659 3912 3974 1391
Size: 43 26 259 119 253 62 4072
Fragments arranged by size:
4072 663 507 259 253 207 180 144
119 85 62 43 26 26 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3675 928
Size: 971 1776 3908
Fragments arranged by size:
3908 1776 971
BbvCI CC'TCA_GC
Cuts at: 3169 3169
Size: 6655
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4846 4880 1903
Size: 34 2909 34 3678
Fragments arranged by size:
3678 2909 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4547 5757 4547
Size: 1210 5445
BclI T'GATC_A
Cuts at: 1548 2118 1548
Size: 570 6085
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3944 5266 136
Size: 107 122 71 631 605 2272 1322 1525
Fragments arranged by size:
2272 1525 1322 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6650 6650
Size: 6655
BlpI GC'TnA_GC
Cuts at: 4072 4072
Size: 6655
Bme1580I G_kGCm'C
Cuts at: 1790 2054 2079 2561 2861 2905 3005 3281 3912
Size: 264 25 482 300 44 100 276 631
Cuts at: 3912 3927 4716 5872 1790
Size: 15 789 1156 2573
Fragments arranged by size:
2573 1156 789 631 482 300 276 264
100 44 25 15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6655
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2631 3231 4386
Size: 416 53 83 399 24 1214 600 1155
Cuts at: 4386 5344 442
Size: 958 1753
Fragments arranged by size:
1753 1214 1155 958 600 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6655
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3002 3002
Size: 6655
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2366 2417 2669 3269 3691 3900 5315 1239
Size: 1127 51 252 600 422 209 1415 2579
Fragments arranged by size:
2579 1415 1127 600 422 252 209 51
Bpu10I CC'TnA_GC
Cuts at: 3169 3169
Size: 6655
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4780 5645 5943 4780
Size: 865 298 5492
Fragments arranged by size:
5492 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2159 2634 2717 3265 5318 882
Size: 617 660 475 83 548 2053 2219
Fragments arranged by size:
2219 2053 660 617 548 475 83
BsaAI yAC'GTr
Cuts at: 493 1204 2065 4446 493
Size: 711 861 2381 2702
Fragments arranged by size:
2702 2381 861 711
BsaBI GATnn'nnATC
Cuts at: 3395 6649 3395
Size: 3254 3401
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3106 3463 4462 4844
Size: 53 83 186 936 1573 357 999 382
Cuts at: 4844 5479 275
Size: 635 1451
Fragments arranged by size:
1573 1451 999 936 635 382 357 186
83 53
BsaWI w'CCGG_w
Cuts at: 5088 5486 5760 5907 6318 5088
Size: 398 274 147 411 5425
Fragments arranged by size:
5425 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2409 2439 2897 2900 2927
Size: 30 165 30 1006 30 458 3 27
Cuts at: 2927 2930 2966 2996 3012 3042 3408 3438 3874
Size: 3 36 30 16 30 366 30 436
Cuts at: 3874 3904 1178
Size: 30 3929
Fragments arranged by size:
3929 1006 458 436 366 165 36 30
30 30 30 30 30 30 27 16
3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2735 2905 3413 3416 3419 3422 3425 3428
Size: 1549 170 508 3 3 3 3 3
Cuts at: 3428 3723 3882 1186
Size: 295 159 3959
Fragments arranged by size:
3959 1549 508 295 170 159 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2391 2396 2514 2828 2933 3281 3571 3849 3926
Size: 5 118 314 105 348 290 278 77
Cuts at: 3926 5858 6354 2391
Size: 1932 496 2692
Fragments arranged by size:
2692 1932 496 348 314 290 278 118
105 77 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2087 2714 3014 3839 1163
Size: 573 351 627 300 825 3979
Fragments arranged by size:
3979 825 627 573 351 300
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4105 4866 5894 1540
Size: 275 186 2104 761 1028 2301
Fragments arranged by size:
2301 2104 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2655 2905 3212 3255 4716 4801 5872 2655
Size: 250 307 43 1461 85 1071 3438
Fragments arranged by size:
3438 1461 1071 307 250 85 43
BsmI GAATG_Cn'
Cuts at: 4166 4259 4166
Size: 93 6562
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2079 2561 2570 2655 2835 2861
Size: 399 264 25 482 9 85 180 26
Cuts at: 2861 2905 3005 3212 3255 3281 3540 3659 3912
Size: 44 100 207 43 26 259 119 253
Cuts at: 3912 3927 3974 4716 4801 5872 1391
Size: 15 47 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 482 399 264 259 253
207 180 119 100 85 85 47 44
43 26 26 25 15 9
BspEI T'CCGG_A
Cuts at: 6318 6318
Size: 6655
BspHI T'CATG_A
Cuts at: 4544 6274 4544
Size: 1730 4925
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2568 3231 4010 844
Size: 1724 663 779 3489
Fragments arranged by size:
3489 1724 779 663
BsrBI CCG'CTC
Cuts at: 1394 1823 2325 2871 4542 1394
Size: 429 502 546 1671 3507
Fragments arranged by size:
3507 1671 546 502 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5150 5324 65
Size: 1183 3902 174 1396
Fragments arranged by size:
3902 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5299 5486 1700
Size: 110 3489 187 2869
Fragments arranged by size:
3489 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6655
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6655
BssSI C'ACGA_G
Cuts at: 3254 4715 5727 3254
Size: 1461 1012 4182
Fragments arranged by size:
4182 1461 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6655
BstBI TT'CG_AA
Cuts at: 5459 5459
Size: 6655
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6655
BstXI CCAn_nnnn'nTGG
Cuts at: 2404 2528 2404
Size: 124 6531
BstYI r'GATC_y
Cuts at: 1972 2220 3352 3985 4352 4737 4754 6206 6292
Size: 248 1132 633 367 385 17 1452 86
Cuts at: 6292 6304 6650 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1132 633 385 367 346 248
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2144 2718 3016 3321 3627 2144
Size: 574 298 305 306 5172
Fragments arranged by size:
5172 574 306 305 298
BtgI C'CryG_G
Cuts at: 513 1065 2403 2527 2553 3006 3101 3153 513
Size: 552 1338 124 26 453 95 52 4015
Fragments arranged by size:
4015 1338 552 453 124 95 52 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5916
Fragments arranged by size:
5916 683 56
BtsI GCAGTG_nn'
Cuts at: 2313 2341 2744 3563 3687 4171 4965 4985 2313
Size: 28 403 819 124 484 794 20 3983
Fragments arranged by size:
3983 819 794 484 403 124 28 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6655
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6620
DraI TTT'AAA
Cuts at: 3959 3959
Size: 6655
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3462 5662 809
Size: 507 2146 2200 1802
Fragments arranged by size:
2200 2146 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3405 3945 4102
Size: 54 1425 50 275 18 1575 540 157
Cuts at: 4102 4991 6620 8
Size: 889 1629 43
Fragments arranged by size:
1629 1575 1425 889 540 275 157 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4102 1537
Size: 275 2290 4090
Fragments arranged by size:
4090 2290 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3446 3780 4585 1353
Size: 2093 334 805 3423
Fragments arranged by size:
3423 2093 805 334
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3797 5242 5616 5762 387
Size: 1064 138 2208 1445 374 146 1280
Fragments arranged by size:
2208 1445 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2653 3210 3253 2653
Size: 557 43 6055
Fragments arranged by size:
6055 557 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2366 2417 2669 2786 3269 3691 3843
Size: 393 734 51 252 117 483 422 152
Cuts at: 3843 3900 4718 5315 6102 6370 1239
Size: 57 818 597 787 268 1524
Fragments arranged by size:
1524 818 787 734 597 483 422 393
268 252 152 117 57 51
EcoO109I rG'GnC_Cy
Cuts at: 2051 2558 2702 2858 3002 3158 3183 3277 3278
Size: 507 144 156 144 156 25 94 1
Cuts at: 3278 3299 3908 3909 2051
Size: 21 609 1 4797
Fragments arranged by size:
4797 609 507 156 156 144 144 94
25 21 1 1
EcoRI G'AATT_C
Cuts at: 3146 3755 3964 4342 3146
Size: 609 209 378 5459
Fragments arranged by size:
5459 609 378 209
FspI TGC'GCA
Cuts at: 5161 5161
Size: 6655
HaeII r_GCGC'y
Cuts at: 1678 5802 1678
Size: 4124 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2355 2387 2557 2589 2597
Size: 32 135 32 783 32 170 32 8
Cuts at: 2597 2629 2716 2748 2966 2998 3634 3666 5296
Size: 32 87 32 218 32 636 32 1630
Cuts at: 5296 5328 5370 5402 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1630 783 636 218 170 135 87
42 32 32 32 32 32 32 32
32 32 32 8
HincII GTy'rAC
Cuts at: 669 1657 1938 3999 4245 669
Size: 988 281 2061 246 3079
Fragments arranged by size:
3079 2061 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6655
HpaI GTT'AAC
Cuts at: 4245 4245
Size: 6655
KpnI G_GTAC'C
Cuts at: 3980 3980
Size: 6655
MfeI C'AATT_G
Cuts at: 4254 4254
Size: 6655
MluI A'CGCG_T
Cuts at: 6531 6531
Size: 6655
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3330 3478 3532 5769 5953 1305
Size: 2025 148 54 2237 184 2007
Fragments arranged by size:
2237 2025 2007 184 148 54
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3947 10
Size: 54 1425 343 2115 2718
Fragments arranged by size:
2718 2115 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2526 3052 3237 3401 4613 4972 5131
Size: 648 1360 526 185 164 1212 359 159
Cuts at: 5131 518
Size: 2042
Fragments arranged by size:
2042 1360 1212 648 526 359 185 164
159
MspA1I CmG'CkG
Cuts at: 1606 3092 3575 3853 3920 4071 4748 5896 6141
Size: 1486 483 278 67 151 677 1148 245
Cuts at: 6141 1606
Size: 2120
Fragments arranged by size:
2120 1486 1148 677 483 278 245 151
67
NcoI C'CATG_G
Cuts at: 513 1065 3101 3153 513
Size: 552 2036 52 4015
Fragments arranged by size:
4015 2036 552 52
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6655
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6655
NotI GC'GGCC_GC
Cuts at: 4102 4102
Size: 6655
NspI r_CATG'y
Cuts at: 1132 1229 2282 4013 5558 1132
Size: 97 1053 1731 1545 2229
Fragments arranged by size:
2229 1731 1545 1053 97
PasI CC'CwG_GG
Cuts at: 1348 2125 2854 1348
Size: 777 729 5149
Fragments arranged by size:
5149 777 729
PciI A'CATG_T
Cuts at: 5554 5554
Size: 6655
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2097 2271 3749 1108
Size: 989 174 1478 4014
Fragments arranged by size:
4014 1478 989 174
PfoI T'CCnGG_A
Cuts at: 2646 3759 2646
Size: 1113 5542
PmeI GTTT'AAAC
Cuts at: 3959 3959
Size: 6655
PmlI CAC'GTG
Cuts at: 2065 2065
Size: 6655
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4724 4756 6237 6269 4724
Size: 32 1481 32 5110
Fragments arranged by size:
5110 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2702 3158 3183 2702
Size: 456 25 6174
Fragments arranged by size:
6174 456 25
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6655
PsiI TTA'TAA
Cuts at: 4225 4225
Size: 6655
PspOMI G'GGCC_C
Cuts at: 2050 2557 2857 3001 3277 3908 2050
Size: 507 300 144 276 631 4797
Fragments arranged by size:
4797 631 507 300 276 144
PstI C_TGCA'G
Cuts at: 830 2320 2739 3520 3558 4007 830
Size: 1490 419 781 38 449 3478
Fragments arranged by size:
3478 1490 781 449 419 38
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6655
PvuII CAG'CTG
Cuts at: 3092 3575 3853 3920 3092
Size: 483 278 67 5827
Fragments arranged by size:
5827 483 278 67
SacI G_AGCT'C
Cuts at: 2655 3212 3255 2655
Size: 557 43 6055
Fragments arranged by size:
6055 557 43
SalI G'TCGA_C
Cuts at: 1936 3997 1936
Size: 2061 4594
SbfI CC_TGCA'GG
Cuts at: 3520 4007 3520
Size: 487 6168
ScaI AGT'ACT
Cuts at: 4903 6615 4903
Size: 1712 4943
SfcI C'TryA_G
Cuts at: 826 1045 2316 2735 3187 3516 3554 4003 5138
Size: 219 1271 419 452 329 38 449 1135
Cuts at: 5138 5819 6010 826
Size: 681 191 1471
Fragments arranged by size:
1471 1271 1135 681 452 449 419 329
219 191 38
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6655
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6655
SmaI CCC'GGG
Cuts at: 1872 3982 1872
Size: 2110 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4759 5660 5922 820
Size: 197 926 2816 901 262 1553
Fragments arranged by size:
2816 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4446 493
Size: 3953 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6655
SphI G_CATG'C
Cuts at: 1132 4013 1132
Size: 2881 3774
SspI AAT'ATT
Cuts at: 5 52 4579 5
Size: 47 4527 2081
Fragments arranged by size:
4527 2081 47
StuI AGG'CCT
Cuts at: 1517 3552 1517
Size: 2035 4620
StyI C'CwwG_G
Cuts at: 513 1065 2662 3078 3101 3153 3713 4094 513
Size: 552 1597 416 23 52 560 381 3074
Fragments arranged by size:
3074 1597 560 552 416 381 52 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2138 2511 4682 4699 4852 5037 5482
Size: 1255 558 373 2171 17 153 185 445
Cuts at: 5482 325
Size: 1498
Fragments arranged by size:
2171 1498 1255 558 445 373 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2886 4901 6613
Size: 274 80 33 51 1443 909 2015 1712
Cuts at: 6613 96
Size: 138
Fragments arranged by size:
2015 1712 1443 909 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2897 2929 3012 3044 6615 6647 2897
Size: 32 83 32 3571 32 2905
Fragments arranged by size:
3571 2905 83 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2978 1316
Size: 1662 4993
XbaI T'CTAG_A
Cuts at: 3991 3991
Size: 6655
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2268 2551 2644 2851 2941 3244 2268
Size: 283 93 207 90 303 5679
Fragments arranged by size:
5679 303 283 207 93 90
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6655
XmaI C'CCGG_G
Cuts at: 1870 3980 1870
Size: 2110 4545
XmnI GAAnn'nnTTC
Cuts at: 4784 4784
Size: 6655
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3464 4463 276
Size: 53 83 186 936 1930 999 2468
Fragments arranged by size:
2468 1930 999 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I
DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI