Restriction map for
pFN21AE1453
(Circular) (Six-base) MAPSORT of: pFN21AE1453.seq Check: 726 from: 1 to: 7891
pFN21AE1453 7891 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3275 3275
Size: 7891
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3392 5701 278
Size: 53 83 186 936 1856 2309 2468
Fragments arranged by size:
2468 2309 1856 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2145 3909 5234 1937
Size: 208 1764 1325 4594
Fragments arranged by size:
4594 1764 1325 208
Acc65I G'GTAC_C
Cuts at: 4683 5212 4683
Size: 529 7362
AclI AA'CG_TT
Cuts at: 1563 6018 6391 1563
Size: 4455 373 3063
Fragments arranged by size:
4455 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2776 3248 5954 7338 7606 1632
Size: 1144 472 2706 1384 268 1917
Fragments arranged by size:
2706 1917 1384 1144 472 268
AfeI AGC'GCT
Cuts at: 2381 2381
Size: 7891
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7694
AflIII A'CryG_T
Cuts at: 1203 5024 6790 7767 1203
Size: 3821 1766 977 1327
Fragments arranged by size:
3821 1766 1327 977
AgeI A'CCGG_T
Cuts at: 6722 6722
Size: 7891
AhdI GACnn_n'nnGTC
Cuts at: 6620 6620
Size: 7891
AleI CACnn'nnGTG
Cuts at: 1166 2630 4621 1166
Size: 1464 1991 4436
Fragments arranged by size:
4436 1991 1464
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3918 1184
Size: 2734 5157
AlwNI CAG_nnn'CTG
Cuts at: 2202 2799 3376 3616 3817 4441 5634 7206 2202
Size: 597 577 240 201 624 1193 1572 2887
Fragments arranged by size:
2887 1572 1193 624 597 577 240 201
ApaI G_GGCC'C
Cuts at: 2616 2768 3025 2616
Size: 152 257 7482
Fragments arranged by size:
7482 257 152
ApaLI G'TGCA_C
Cuts at: 3217 5948 7104 3217
Size: 2731 1156 4004
Fragments arranged by size:
4004 2731 1156
ApoI r'AATT_y
Cuts at: 1493 2717 5200 5426 5578 7687 1493
Size: 1224 2483 226 152 2109 1697
Fragments arranged by size:
2483 2109 1697 1224 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6445 160
Size: 872 5413 1606
Fragments arranged by size:
5413 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7891
AvaI C'yCGr_G
Cuts at: 1870 1943 2609 3422 4367 4688 5216 1870
Size: 73 666 813 945 321 528 4545
Fragments arranged by size:
4545 945 813 666 528 321 73
BamHI G'GATC_C
Cuts at: 3039 4155 5221 5588 3039
Size: 1116 1066 367 5342
Fragments arranged by size:
5342 1116 1066 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2944 4683 5036 5212 6667
Size: 325 217 627 1157 1739 353 176 1455
Cuts at: 6667 618
Size: 1842
Fragments arranged by size:
1842 1739 1455 1157 627 353 325 217
176
BanII G_rGCy'C
Cuts at: 1391 2121 2616 2754 2768 3025 3191 5210 1391
Size: 730 495 138 14 257 166 2019 4072
Fragments arranged by size:
4072 2019 730 495 257 166 138 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2004 2504 2782 4926 928
Size: 971 105 500 278 2144 3893
Fragments arranged by size:
3893 2144 971 500 278 105
BbvCI CC'TCA_GC
Cuts at: 2738 3515 4937 2738
Size: 777 1422 5692
Fragments arranged by size:
5692 1422 777
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6082 6116 1903
Size: 34 4145 34 3678
Fragments arranged by size:
4145 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5783 6993 5783
Size: 1210 6681
BclI T'GATC_A
Cuts at: 1548 2091 4045 1548
Size: 543 1954 5394
Fragments arranged by size:
5394 1954 543
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2763 6502 136
Size: 107 122 71 631 605 1091 3739 1525
Fragments arranged by size:
3739 1525 1091 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7886 7886
Size: 7891
BlpI GC'TnA_GC
Cuts at: 2057 2753 3353 5308 2057
Size: 696 600 1955 4640
Fragments arranged by size:
4640 1955 696 600
Bme1580I G_kGCm'C
Cuts at: 1790 2616 2768 2949 3025 3221 5952 7108 1790
Size: 826 152 181 76 196 2731 1156 2573
Fragments arranged by size:
2731 2573 1156 826 196 181 152 76
BmgBI CAC'GTC
Cuts at: 1318 2362 4446 1318
Size: 1044 2084 4763
Fragments arranged by size:
4763 2084 1044
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2212 2701 2935
Size: 416 53 83 399 24 795 489 234
Cuts at: 2935 5622 6580 442
Size: 2687 958 1753
Fragments arranged by size:
2687 1753 958 795 489 416 399 234
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7891
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3485 3485
Size: 7891
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 2105 2207 2396 2553 3104 3575 4178
Size: 830 36 102 189 157 551 471 603
Cuts at: 4178 6551 1239
Size: 2373 2579
Fragments arranged by size:
2579 2373 830 603 551 471 189 157
102 36
Bpu10I CC'TnA_GC
Cuts at: 2738 3515 4937 2738
Size: 777 1422 5692
Fragments arranged by size:
5692 1422 777
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4357 4699 4891 6016 6881 7179 4357
Size: 342 192 1125 865 298 5069
Fragments arranged by size:
5069 1125 865 342 298 192
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6554 882
Size: 617 5055 2219
Fragments arranged by size:
5055 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5682 493
Size: 711 4478 2702
Fragments arranged by size:
4478 2702 711
BsaBI GATnn'nnATC
Cuts at: 2579 4233 7885 2579
Size: 1654 3652 2585
Fragments arranged by size:
3652 2585 1654
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3389 4020 5698 6080
Size: 53 83 186 936 1856 631 1678 382
Cuts at: 6080 6715 275
Size: 635 1451
Fragments arranged by size:
1856 1678 1451 936 635 631 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3036 3623 3739 5043 6324 6722 6996 7143 7554
Size: 587 116 1304 1281 398 274 147 411
Cuts at: 7554 3036
Size: 3373
Fragments arranged by size:
3373 1304 1281 587 411 398 274 147
116
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2103 2133 3470 3500 1178
Size: 30 165 30 700 30 1337 30 5569
Fragments arranged by size:
5569 1337 700 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3065 3101 3175 3578 3860 3863 1186
Size: 1879 36 74 403 282 3 5214
Fragments arranged by size:
5214 1879 403 282 74 36 3
BseYI C'CCAG_C
Cuts at: 2881 3184 4222 7094 7590 2881
Size: 303 1038 2872 496 3182
Fragments arranged by size:
3182 2872 1038 496 303
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2503 2791 3449 3878 1163
Size: 573 767 288 658 429 5176
Fragments arranged by size:
5176 767 658 573 429 288
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5341 6102 7130 1540
Size: 275 186 3340 761 1028 2301
Fragments arranged by size:
3340 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2121 2754 3221 5952 6037 7108 2121
Size: 633 467 2731 85 1071 2904
Fragments arranged by size:
2904 2731 1071 633 467 85
BsmI GAATG_Cn'
Cuts at: 5402 5495 5402
Size: 93 7798
BsmBI CGTCTCn'nnnn_
Cuts at: 3117 3117
Size: 7891
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2616 2754 2768 2949 3025 3191
Size: 399 331 495 138 14 181 76 166
Cuts at: 3191 3221 5210 5952 6037 7108 1391
Size: 30 1989 742 85 1071 2174
Fragments arranged by size:
2174 1989 1071 742 495 399 331 181
166 138 85 76 30 14
BspEI T'CCGG_A
Cuts at: 3036 3623 5043 7554 3036
Size: 587 1420 2511 3373
Fragments arranged by size:
3373 2511 1420 587
BspHI T'CATG_A
Cuts at: 5176 5184 5780 7510 5176
Size: 8 596 1730 5557
Fragments arranged by size:
5557 1730 596 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2043 2475 3275 3816 3960 3961 4365 5246
Size: 1199 432 800 541 144 1 404 881
Cuts at: 5246 844
Size: 3489
Fragments arranged by size:
3489 1199 881 800 541 432 404 144
1
BsrBI CCG'CTC
Cuts at: 1394 1823 5778 1394
Size: 429 3955 3507
Fragments arranged by size:
3955 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4303 6386 6560 65
Size: 1183 3055 2083 174 1396
Fragments arranged by size:
3055 2083 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2941 3309 6535 6722 1700
Size: 110 1131 368 3226 187 2869
Fragments arranged by size:
3226 2869 1131 368 187 110
BsrGI T'GTAC_A
Cuts at: 96 4497 96
Size: 4401 3490
BssHII G'CGCG_C
Cuts at: 1925 3339 1925
Size: 1414 6477
BssSI C'ACGA_G
Cuts at: 2114 5951 6963 2114
Size: 3837 1012 3042
Fragments arranged by size:
3837 3042 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3817 794
Size: 3023 4868
BstBI TT'CG_AA
Cuts at: 6695 6695
Size: 7891
BstEII G'GTnAC_C
Cuts at: 1184 3004 1184
Size: 1820 6071
BstXI CCAn_nnnn'nTGG
Cuts at: 3709 3709
Size: 7891
BstYI r'GATC_y
Cuts at: 1972 2127 2251 3039 3409 4034 4102 4155 4795
Size: 155 124 788 370 625 68 53 640
Cuts at: 4795 5221 5588 5973 5990 7442 7528 7540 7886
Size: 426 367 385 17 1452 86 12 346
Cuts at: 7886 1972
Size: 1977
Fragments arranged by size:
1977 1452 788 640 625 426 385 370
367 346 155 124 86 68 53 17
12
Bsu36I CC'TnA_GG
Cuts at: 3026 3026
Size: 7891
BtgI C'CryG_G
Cuts at: 513 1065 2004 3198 513
Size: 552 939 1194 5206
Fragments arranged by size:
5206 1194 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3778 4752 487
Size: 683 56 2552 974 3626
Fragments arranged by size:
3626 2552 974 683 56
BtsI GCAGTG_nn'
Cuts at: 3534 3965 4002 4406 5407 6201 6221 3534
Size: 431 37 404 1001 794 20 5204
Fragments arranged by size:
5204 1001 794 431 404 37 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7891
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7856
DraI TTT'AAA
Cuts at: 2452 5195 2452
Size: 2743 5148
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6898 809
Size: 507 5582 1802
Fragments arranged by size:
5582 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3363 3583 4629
Size: 54 1425 50 275 18 1533 220 1046
Cuts at: 4629 5338 6227 7856 8
Size: 709 889 1629 43
Fragments arranged by size:
1629 1533 1425 1046 889 709 275 220
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5338 1537
Size: 275 3526 4090
Fragments arranged by size:
4090 3526 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2516 3564 3689 4260 5821 1353
Size: 1163 1048 125 571 1561 3423
Fragments arranged by size:
3423 1561 1163 1048 571 125
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3062 3461 6478 6852 6998 387
Size: 1064 138 1473 399 3017 374 146 1280
Fragments arranged by size:
3017 1473 1280 1064 399 374 146 138
EcoICRI GAG'CTC
Cuts at: 2119 2752 2119
Size: 633 7258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2105 2207 2396 2553 2776 3104
Size: 393 437 36 102 189 157 223 328
Cuts at: 3104 3248 3575 4178 5954 6551 7338 7606 1239
Size: 144 327 603 1776 597 787 268 1524
Fragments arranged by size:
1776 1524 787 603 597 437 393 328
327 268 223 189 157 144 102 36
EcoNI CCTnn'n_nnAGG
Cuts at: 3047 3086 3473 3557 4841 3047
Size: 39 387 84 1284 6097
Fragments arranged by size:
6097 1284 387 84 39
EcoO109I rG'GnC_Cy
Cuts at: 2613 3021 3022 3031 3099 3253 3373 4579 2613
Size: 408 1 9 68 154 120 1206 5925
Fragments arranged by size:
5925 1206 408 154 120 68 9 1
EcoRI G'AATT_C
Cuts at: 5200 5578 5200
Size: 378 7513
EcoRV GAT'ATC
Cuts at: 4364 4364
Size: 7891
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5187 5187
Size: 7891
FspI TGC'GCA
Cuts at: 6397 6397
Size: 7891
HaeII r_GCGC'y
Cuts at: 1678 2383 2903 7038 1678
Size: 705 520 4135 2531
Fragments arranged by size:
4135 2531 705 520
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3470 3502 4884 4913 4916
Size: 32 135 32 1898 32 1382 29 3
Cuts at: 4916 4945 6532 6564 6606 6638 1373
Size: 29 1587 32 42 32 2626
Fragments arranged by size:
2626 1898 1587 1382 135 42 32 32
32 32 32 29 29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 4432 5235 5481 669
Size: 988 281 2494 803 246 3079
Fragments arranged by size:
3079 2494 988 803 281 246
HindIII A'AGCT_T
Cuts at: 748 4760 748
Size: 4012 3879
HpaI GTT'AAC
Cuts at: 5481 5481
Size: 7891
KpnI G_GTAC'C
Cuts at: 4687 5216 4687
Size: 529 7362
MfeI C'AATT_G
Cuts at: 4570 5490 4570
Size: 920 6971
MluI A'CGCG_T
Cuts at: 7767 7767
Size: 7891
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3462 3690 4485 4524 7005 7189 1305
Size: 2157 228 795 39 2481 184 2007
Fragments arranged by size:
2481 2157 2007 795 228 184 39
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4631 10
Size: 54 1425 343 2799 3270
Fragments arranged by size:
3270 2799 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2630 3068 3900 4621 5849 6208 6367
Size: 648 1464 438 832 721 1228 359 159
Cuts at: 6367 518
Size: 2042
Fragments arranged by size:
2042 1464 1228 832 721 648 438 359
159
MspA1I CmG'CkG
Cuts at: 1606 2742 3184 3200 3331 4226 4941 5307 5984
Size: 1136 442 16 131 895 715 366 677
Cuts at: 5984 7132 7377 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1136 895 715 677 442 366
245 131 16
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6400
Fragments arranged by size:
6400 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7891
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7891
NotI GC'GGCC_GC
Cuts at: 5338 5338
Size: 7891
NspI r_CATG'y
Cuts at: 1132 1229 4057 5028 5249 6794 1132
Size: 97 2828 971 221 1545 2229
Fragments arranged by size:
2828 2229 1545 971 221 97
PasI CC'CwG_GG
Cuts at: 1348 2598 3018 5154 1348
Size: 1250 420 2136 4085
Fragments arranged by size:
4085 2136 1250 420
PciI A'CATG_T
Cuts at: 5024 6790 5024
Size: 1766 6125
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2557 4201 1108
Size: 1449 1644 4798
Fragments arranged by size:
4798 1644 1449
PfoI T'CCnGG_A
Cuts at: 2046 2508 3081 3453 3990 2046
Size: 462 573 372 537 5947
Fragments arranged by size:
5947 573 537 462 372
PmeI GTTT'AAAC
Cuts at: 5195 5195
Size: 7891
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5960 5992 7473 7505 5960
Size: 32 1481 32 6346
Fragments arranged by size:
6346 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3031 3253 4579 3031
Size: 222 1326 6343
Fragments arranged by size:
6343 1326 222
PshAI GACnn'nnGTC
Cuts at: 1613 3444 4185 4971 1613
Size: 1831 741 786 4533
Fragments arranged by size:
4533 1831 786 741
PsiI TTA'TAA
Cuts at: 5461 5461
Size: 7891
PspOMI G'GGCC_C
Cuts at: 2612 2764 3021 2612
Size: 152 257 7482
Fragments arranged by size:
7482 257 152
PstI C_TGCA'G
Cuts at: 830 2040 2650 2747 3237 3308 3813 3957 3972
Size: 1210 610 97 490 71 505 144 15
Cuts at: 3972 4533 4704 5243 830
Size: 561 171 539 3478
Fragments arranged by size:
3478 1210 610 561 539 505 490 171
144 97 71 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7891
PvuII CAG'CTG
Cuts at: 2742 3184 3331 4226 4941 2742
Size: 442 147 895 715 5692
Fragments arranged by size:
5692 895 715 442 147
SacI G_AGCT'C
Cuts at: 2121 2754 2121
Size: 633 7258
SacII CC_GC'GG
Cuts at: 3201 3201
Size: 7891
SalI G'TCGA_C
Cuts at: 1936 5233 1936
Size: 3297 4594
SapI GCTCTTCn'nnn_
Cuts at: 2516 4260 2516
Size: 1744 6147
SbfI CC_TGCA'GG
Cuts at: 2040 2650 3813 5243 2040
Size: 610 1163 1430 4688
Fragments arranged by size:
4688 1430 1163 610
ScaI AGT'ACT
Cuts at: 6139 7851 6139
Size: 1712 6179
SfcI C'TryA_G
Cuts at: 826 1045 2036 2646 2743 3233 3304 3809 3953
Size: 219 991 610 97 490 71 505 144
Cuts at: 3953 3968 4529 4700 5239 6374 7055 7246 826
Size: 15 561 171 539 1135 681 191 1471
Fragments arranged by size:
1471 1135 991 681 610 561 539 505
490 219 191 171 144 97 71 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7891
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7891
SmaI CCC'GGG
Cuts at: 1872 2611 5218 1872
Size: 739 2607 4545
Fragments arranged by size:
4545 2607 739
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4336 4678 4870 5995 6896 7158
Size: 197 926 2393 342 192 1125 901 262
Cuts at: 7158 820
Size: 1553
Fragments arranged by size:
2393 1553 1125 926 901 342 262 197
192
SnaBI TAC'GTA
Cuts at: 493 5682 493
Size: 5189 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7891
SphI G_CATG'C
Cuts at: 1132 5249 1132
Size: 4117 3774
SspI AAT'ATT
Cuts at: 5 52 5815 5
Size: 47 5763 2081
Fragments arranged by size:
5763 2081 47
StuI AGG'CCT
Cuts at: 1517 2652 5167 1517
Size: 1135 2515 4241
Fragments arranged by size:
4241 2515 1135
StyI C'CwwG_G
Cuts at: 513 1065 2004 2653 2988 3702 4416 4707 4983
Size: 552 939 649 335 714 714 291 276
Cuts at: 4983 5330 513
Size: 347 3074
Fragments arranged by size:
3074 939 714 714 649 552 347 335
291 276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2194 2201 5918 5935 6088 6273 6718
Size: 1255 614 7 3717 17 153 185 445
Cuts at: 6718 325
Size: 1498
Fragments arranged by size:
3717 1498 1255 614 445 185 153 17
7
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4497 6137 7849
Size: 274 80 33 51 1443 2520 1640 1712
Cuts at: 7849 96
Size: 138
Fragments arranged by size:
2520 1712 1640 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2103 2135 4147 4179 7851 7883 2103
Size: 32 2012 32 3672 32 2111
Fragments arranged by size:
3672 2111 2012 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3257 3482 1316
Size: 1941 225 5725
Fragments arranged by size:
5725 1941 225
XbaI T'CTAG_A
Cuts at: 2356 5227 2356
Size: 2871 5020
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7891
XmaI C'CCGG_G
Cuts at: 1870 2609 5216 1870
Size: 739 2607 4545
Fragments arranged by size:
4545 2607 739
XmnI GAAnn'nnTTC
Cuts at: 6020 6020
Size: 7891
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3390 5699 276
Size: 53 83 186 936 1856 2309 2468
Fragments arranged by size:
2468 2309 1856 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI