Restriction map for
pFN21AE1452
(Circular) (Six-base) MAPSORT of: pFN21AE1452.seq Check: 9496 from: 1 to: 5452
pFN21AE1452 5452 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3262 278
Size: 53 83 186 936 1726 2468
Fragments arranged by size:
2468 1726 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2795 1937
Size: 858 4594
Acc65I G'GTAC_C
Cuts at: 2773 2773
Size: 5452
AclI AA'CG_TT
Cuts at: 1563 3579 3952 1563
Size: 2016 373 3063
Fragments arranged by size:
3063 2016 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2195 2717 3515 4899 5167 1632
Size: 563 522 798 1384 268 1917
Fragments arranged by size:
1917 1384 798 563 522 268
AfeI AGC'GCT
Cuts at: 2351 2351
Size: 5452
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5255
AflIII A'CryG_T
Cuts at: 1203 4351 5328 1203
Size: 3148 977 1327
Fragments arranged by size:
3148 1327 977
AgeI A'CCGG_T
Cuts at: 4283 4283
Size: 5452
AhdI GACnn_n'nnGTC
Cuts at: 4181 4181
Size: 5452
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5452
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5452
AlwNI CAG_nnn'CTG
Cuts at: 2201 3195 4767 2201
Size: 994 1572 2886
Fragments arranged by size:
2886 1572 994
ApaLI G'TGCA_C
Cuts at: 2606 3509 4665 2606
Size: 903 1156 3393
Fragments arranged by size:
3393 1156 903
ApoI r'AATT_y
Cuts at: 1493 2761 2987 3139 5248 1493
Size: 1268 226 152 2109 1697
Fragments arranged by size:
2109 1697 1268 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4006 160
Size: 872 2974 1606
Fragments arranged by size:
2974 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5452
AvaI C'yCGr_G
Cuts at: 1870 1943 2027 2090 2777 1870
Size: 73 84 63 687 4545
Fragments arranged by size:
4545 687 84 73 63
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2053 2086 2053
Size: 33 5419
BamHI G'GATC_C
Cuts at: 2782 3149 2782
Size: 367 5085
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2773 4228 618
Size: 325 217 627 986 1455 1842
Fragments arranged by size:
1842 1455 986 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2771 1391
Size: 1380 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2067 2262 2295 928
Size: 971 168 195 33 4085
Fragments arranged by size:
4085 971 195 168 33
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2405 2439 3643 3677 1903
Size: 34 468 34 1204 34 3678
Fragments arranged by size:
3678 1204 468 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3344 4554 3344
Size: 1210 4242
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5452
BfrBI ATG'CAT
Cuts at: 2129 2129
Size: 5452
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4063 136
Size: 107 122 71 631 605 2391 1525
Fragments arranged by size:
2391 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5447 5447
Size: 5452
BlpI GC'TnA_GC
Cuts at: 2869 2869
Size: 5452
Bme1580I G_kGCm'C
Cuts at: 1790 2610 3513 4669 1790
Size: 820 903 1156 2573
Fragments arranged by size:
2573 1156 903 820
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5452
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2189 2306 3183
Size: 416 53 83 399 24 772 117 877
Cuts at: 3183 4141 442
Size: 958 1753
Fragments arranged by size:
1753 958 877 772 416 399 117 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5452
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4112 1239
Size: 2873 2579
Bpu10I CC'TnA_GC
Cuts at: 2015 2015
Size: 5452
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2185 2699 3577 4442 4740 2185
Size: 514 878 865 298 2897
Fragments arranged by size:
2897 878 865 514 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4115 882
Size: 617 2616 2219
Fragments arranged by size:
2616 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3243 493
Size: 711 2039 2702
Fragments arranged by size:
2702 2039 711
BsaBI GATnn'nnATC
Cuts at: 5446 5446
Size: 5452
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3259 3641 4276 275
Size: 53 83 186 936 1726 382 635 1451
Fragments arranged by size:
1726 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3885 4283 4557 4704 5115 3885
Size: 398 274 147 411 4222
Fragments arranged by size:
4222 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2442 2456 2472 2486 1178
Size: 30 165 30 1039 14 16 14 4144
Fragments arranged by size:
4144 1039 165 30 30 16 14 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2243 1186
Size: 1057 4395
BseYI C'CCAG_C
Cuts at: 4655 5151 4655
Size: 496 4956
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4879
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2902 3663 4691 1540
Size: 275 186 901 761 1028 2301
Fragments arranged by size:
2301 1028 901 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2610 3513 3598 4669 2610
Size: 903 85 1071 3393
Fragments arranged by size:
3393 1071 903 85
BsmI GAATG_Cn'
Cuts at: 2963 3056 2963
Size: 93 5359
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2610 2771 3513 3598 4669 1391
Size: 399 820 161 742 85 1071 2174
Fragments arranged by size:
2174 1071 820 742 399 161 85
BspEI T'CCGG_A
Cuts at: 5115 5115
Size: 5452
BspHI T'CATG_A
Cuts at: 3341 5071 3341
Size: 1730 3722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2094 2807 844
Size: 1250 713 3489
Fragments arranged by size:
3489 1250 713
BsrBI CCG'CTC
Cuts at: 1394 1823 2233 3339 1394
Size: 429 410 1106 3507
Fragments arranged by size:
3507 1106 429 410
BsrDI GCAATG_nn'
Cuts at: 65 1248 2329 2671 3947 4121 65
Size: 1183 1081 342 1276 174 1396
Fragments arranged by size:
1396 1276 1183 1081 342 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4096 4283 1700
Size: 110 2286 187 2869
Fragments arranged by size:
2869 2286 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5452
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5452
BssSI C'ACGA_G
Cuts at: 2609 3512 4524 2609
Size: 903 1012 3537
Fragments arranged by size:
3537 1012 903
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2605 794
Size: 1811 3641
BstBI TT'CG_AA
Cuts at: 4256 4256
Size: 5452
BstEII G'GTnAC_C
Cuts at: 1184 2395 1184
Size: 1211 4241
BstYI r'GATC_y
Cuts at: 1972 2658 2782 3149 3534 3551 5003 5089 5101
Size: 686 124 367 385 17 1452 86 12
Cuts at: 5101 5447 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 686 385 367 346 124 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3961
Fragments arranged by size:
3961 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4713
Fragments arranged by size:
4713 683 56
BtsI GCAGTG_nn'
Cuts at: 2562 2968 3762 3782 2562
Size: 406 794 20 4232
Fragments arranged by size:
4232 794 406 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5452
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5417
DraI TTT'AAA
Cuts at: 2136 2756 2136
Size: 620 4832
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4459 809
Size: 507 3143 1802
Fragments arranged by size:
3143 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2899 3788
Size: 54 1425 50 275 18 177 892 889
Cuts at: 3788 5417 8
Size: 1629 43
Fragments arranged by size:
1629 1425 892 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2899 1537
Size: 275 1087 4090
Fragments arranged by size:
4090 1087 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3382 1353
Size: 2029 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4039 4413 4559 387
Size: 1064 138 2450 374 146 1280
Fragments arranged by size:
2450 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2717 3515 4112 4899 5167 1239
Size: 393 563 522 798 597 787 268 1524
Fragments arranged by size:
1524 798 787 597 563 522 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2179 2297 2179
Size: 118 5334
EcoRI G'AATT_C
Cuts at: 2761 3139 2761
Size: 378 5074
FspI TGC'GCA
Cuts at: 3958 3958
Size: 5452
HaeII r_GCGC'y
Cuts at: 1678 2353 4599 1678
Size: 675 2246 2531
Fragments arranged by size:
2531 2246 675
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2442 2474 4093 4125 4167
Size: 32 135 32 870 32 1619 32 42
Cuts at: 4167 4199 1373
Size: 32 2626
Fragments arranged by size:
2626 1619 870 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2796 3042 669
Size: 988 281 858 246 3079
Fragments arranged by size:
3079 988 858 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5452
HpaI GTT'AAC
Cuts at: 3042 3042
Size: 5452
KpnI G_GTAC'C
Cuts at: 2777 2777
Size: 5452
MfeI C'AATT_G
Cuts at: 3051 3051
Size: 5452
MluI A'CGCG_T
Cuts at: 5328 5328
Size: 5452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4566 4750 1305
Size: 3261 184 2007
Fragments arranged by size:
3261 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3453
Fragments arranged by size:
3453 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2195 2586 3410 3769 3928 518
Size: 648 843 186 391 824 359 159 2042
Fragments arranged by size:
2042 843 824 648 391 359 186 159
MspA1I CmG'CkG
Cuts at: 1606 2413 2602 2868 3545 4693 4938 1606
Size: 807 189 266 677 1148 245 2120
Fragments arranged by size:
2120 1148 807 677 266 245 189
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3961
Fragments arranged by size:
3961 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5452
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5452
NotI GC'GGCC_GC
Cuts at: 2899 2899
Size: 5452
NsiI A_TGCA'T
Cuts at: 2131 2131
Size: 5452
NspI r_CATG'y
Cuts at: 1132 1229 2810 4355 1132
Size: 97 1581 1545 2229
Fragments arranged by size:
2229 1581 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2724 1348
Size: 1376 4076
PciI A'CATG_T
Cuts at: 4351 4351
Size: 5452
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5452
PfoI T'CCnGG_A
Cuts at: 2460 2460
Size: 5452
PmeI GTTT'AAAC
Cuts at: 2756 2756
Size: 5452
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3521 3553 5034 5066 3521
Size: 32 1481 32 3907
Fragments arranged by size:
3907 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2297 2297
Size: 5452
PshAI GACnn'nnGTC
Cuts at: 1613 2295 1613
Size: 682 4770
PsiI TTA'TAA
Cuts at: 3022 3022
Size: 5452
PstI C_TGCA'G
Cuts at: 830 2349 2418 2804 830
Size: 1519 69 386 3478
Fragments arranged by size:
3478 1519 386 69
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5452
PvuII CAG'CTG
Cuts at: 2413 2602 2413
Size: 189 5263
SalI G'TCGA_C
Cuts at: 1936 2794 1936
Size: 858 4594
SbfI CC_TGCA'GG
Cuts at: 2804 2804
Size: 5452
ScaI AGT'ACT
Cuts at: 3700 5412 3700
Size: 1712 3740
SexAI A'CCwGG_T
Cuts at: 2391 2402 2391
Size: 11 5441
SfcI C'TryA_G
Cuts at: 826 1045 2345 2414 2800 3935 4616 4807 826
Size: 219 1300 69 386 1135 681 191 1471
Fragments arranged by size:
1471 1300 1135 681 386 219 191 69
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5452
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5452
SmaI CCC'GGG
Cuts at: 1872 2779 1872
Size: 907 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2164 2714 3556 4457 4719 820
Size: 197 926 221 550 842 901 262 1553
Fragments arranged by size:
1553 926 901 842 550 262 221 197
SnaBI TAC'GTA
Cuts at: 493 3243 493
Size: 2750 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5452
SphI G_CATG'C
Cuts at: 1132 2810 1132
Size: 1678 3774
SspI AAT'ATT
Cuts at: 5 52 3376 5
Size: 47 3324 2081
Fragments arranged by size:
3324 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5452
StyI C'CwwG_G
Cuts at: 513 1065 2004 2040 2891 513
Size: 552 939 36 851 3074
Fragments arranged by size:
3074 939 851 552 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2576 3479 3496 3649 3834 4279 325
Size: 1255 996 903 17 153 185 445 1498
Fragments arranged by size:
1498 1255 996 903 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3698 5410 96
Size: 274 80 33 51 1443 1721 1712 138
Fragments arranged by size:
1721 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5412 5444 5412
Size: 32 5420
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5452
XbaI T'CTAG_A
Cuts at: 2788 2788
Size: 5452
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5452
XmaI C'CCGG_G
Cuts at: 1870 2777 1870
Size: 907 4545
XmnI GAAnn'nnTTC
Cuts at: 2702 3581 2702
Size: 879 4573
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3260 276
Size: 53 83 186 936 1726 2468
Fragments arranged by size:
2468 1726 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI